Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 589, Issue -, Pages 35-40Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2021.12.003
Keywords
BEAS-2B; Mfa1 fimbria; Porphyromonasgingivalis; TLR2; TLR4
Categories
Funding
- JSPS KAKENHI [19K10078, 19K10097, 20K09931]
- Uemura Fund, Dental Research Center, Nihon University School of Dentistry
- Nihon University
- Grants-in-Aid for Scientific Research [20K09931, 19K10078, 19K10097] Funding Source: KAKEN
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The study demonstrates that Porphyromonas gingivalis Mfa1 protein can interact functionally with human bronchial epithelial cells, inducing an inflammatory response involving IL-8 and IL-6 gene expression and protein levels. Specifically, Mfa1-TLR2 showed a functional interaction, suggesting a potential role in pulmonary disease development.
Porphyromonas gingivalis (Pg) a major periodontal pathogen involved in periodontal disease development and progression. Moreover, Pg has two fimbriae surface proteins (FimA and Mfa1) that are genetically distinct and make-up the fimbrial shaft which in-turn form crucial attachment to oral bacteria and multiple host cells. However, unlike FimA, Mfa1 attachment to non-periodontal cells has not been fully elucidated. Considering Pg-associated periodontal disease contributes to pulmonary disease development, we investigated whether Mfa1 can functionally interact with human bronchial epithelial cells and, likewise, trigger a functional response. Initially, we simulated molecular docking and performed both luciferase and neutralization assays to confirm Mfa1-related functional interaction. Subsequently, we treated BEAS-2B cells with purified Mfa1 and performed cytokine quantification through real time-PCR and ELISA to establish Mfa1-related functional response. We found that both Mfa1-TLR2 and Mfa1-TLR4 docking is possible, however, only Mfa1-TLR2 showed a functional interaction. Additionally, we observed that both IL-8 and IL-6 gene expression and protein levels were induced confirming Mfa1-related functional response. Taken together, we propose that BEAS-2B human bronchial epithelial cells are able to recognize Pg Mfa1 and induce both IL-8 and IL-6 inflammatory responses. (c) 2021 Elsevier Inc. All rights reserved.
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