Journal
AUTOIMMUNITY
Volume 55, Issue 1, Pages 43-51Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08916934.2021.1992754
Keywords
Myocardial infarction; myocardial remodelling; microRNA-17-5p; STAT3; autophagy; myocardial fibrosis; cardiomyocyte apoptosis
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miR-17-5p regulates myocardial remodeling after myocardial infarction by targeting STAT3, leading to protection of the myocardium. Inhibiting the expression of miR-17-5p can alleviate myocardial fibrosis and cardiomyocyte apoptosis.
MicroRNAs (miRs) are reported to regulate myocardial infarction (MI). This study was performed to investigate the function and mechanism of miR-17-5p in myocardial remodelling after MI. Initially, a mouse model of MI was established and MI mice were infected with lentivirus antago-miR-17-5p vector. High expression of miR-17-5p was found in myocardial tissues after MI. After inhibiting miR-17-5p expression, myocardial fibrosis, scarring, and cardiomyocyte apoptosis were improved, LC3-II/LC3-I ratio and Beclin-1 expression were decreased but p62 expression was increased. The dual-luciferase assay suggested that miR-17-5p targeted STAT3 and negatively regulated its expression. Then, after inhibiting STAT3 expression using STAT3 inhibitor S31-201, the fibrosis, scarring, and cardiomyocyte apoptosis were deteriorated, along with the rise of LC3-II/LC3-I and Beclin-1 expression, the reduction of p62 expression and the reversion of MI attenuation. In conclusion, inhibition of miR-17-5p can inhibit myocardial autophagy through targeting STAT3 and then inhibit myocardial remodelling, thereby protecting the myocardium after MI.
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