Article
Chemistry, Analytical
Sebastian Schmidtsdorff, Jonas Neumann, Alexander H. Schmidt, Maria K. Parr
Summary: The study demonstrates how lifecycle management can develop and improve suitable and universal analytical methods within short time, with a focus on nitrosamine investigation. The resulting SFC-MS/MS method showed successful analysis for various drug substances and drug products, showcasing high adaptability and the ability to meet changing regulatory requirements.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Review
Food Science & Technology
H. Zamani, M. E. J. R. de Joode, I. J. Hossein, N. F. T. Henckens, M. A. Guggeis, J. E. Berends, T. M. C. M. de Kok, S. G. J. van Breda
Summary: Beetroot juice, rich in nitrate that can be converted to nitric oxide, has potential applications in preventing and treating cardiovascular diseases. It also affects muscle oxygen delivery and endurance, positively impacting sports performances. However, excessive nitrate intake may pose health risks.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2021)
Article
Chemistry, Analytical
Jakub Jires, Michal Dousa, Petr Gibala, Tomas Kubelka
Summary: The possibility of N-nitrosation in the absence of nitrosating agents was studied on model solutions and film coated tablets containing metformin. Alternative routes to N-nitrosation were found, where dimethylamine can undergo oxidation to nitrite in the presence of strong oxidants, followed by the nitrite acting as a nitrosating agent. The described reaction took place in both model solutions and tablets, but to a small degree in the tablets. Adjusting the pH of the solid dosage forms remains an effective measure of controlling N-nitrosamines in the product.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Applied
Elena Keuleyan, Aline Bonifacie, Thierry Sayd, Angeline Duval, Laurent Aubry, Sylvie Bourillon, Philippe Gatellier, Aurelie Promeyrat, Gilles Nassy, Valerie Scislowski, Laurent Picgirard, Laetitia Theron, Veronique Sante-Lhoutellier
Summary: In this study, in vitro digestions of dry-cured sausages formulated with different rates of sodium nitrite and sodium nitrate were performed. The results showed that the chemical reactivity of toxic nitroso-compounds was not affected by the addition of nitrite and nitrate. The concentrations of non-volatile N-nitrosamines increased in the gastric compartment, while nitrites and nitrates remained stable. A minimum rate of 80/80 ppm nitrite/nitrate was sufficient to protect against lipid oxidation in the digestive tract.
Article
Multidisciplinary Sciences
Kyung-In Joung, Jung Eun Hwang, In-Sun Oh, Sung-il Cho, Ju-Young Shin
Summary: A cohort study conducted in South Korea found that the use of ranitidine is not associated with an increased risk of overall cancer or major individual cancers. These findings should be interpreted with caution due to the insufficient follow-up period.
SCIENTIFIC REPORTS
(2022)
Article
Food Science & Technology
Elena Keuleyan, Aline Bonifacie, Philippe Gatellier, Claude Ferreira, Sylvie Blinet, Aurelie Promeyrat, Gilles Nassy, Veronique Sante-Lhoutellier, Laetitia Theron
Summary: Processed meats' nutritional quality can be enhanced by bioactive vegetable molecules, which prevent the synthesis of harmful substances. This study designed an in vitro experimental method to predict the effects of polyphenols and vitamins on chemical reactivity during processed meats' digestion.
Article
Chemistry, Medicinal
Ruth Boetzel, Joerg Schlingemann, Sebastian Hickert, Christian Korn, Grace Kocks, Bert Luck, Giorgio Blom, Mark Harrison, Marc Francois, Leonardo Allain, Yongmei Wu, Youssi Bousraf
Summary: The assessment and control of N-nitrosamine risk have become important in pharmaceutical drug development and quality evaluation. Limited validated information on nitrite levels in excipients has hindered scientists from assessing the risk of nitrosamine formation in pharmaceutical products. A database has been created to store and share this information to provide a common framework for estimating the risk posed by nitrites.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Medicinal
Nikola Pluym, Gerhard Scherer, Jeffery S. Edmiston, Xiaohong C. Jin, Mohamadi Sarkar, Max Scherer
Summary: NNN, a human carcinogen found in tobacco products, can be more accurately assessed by measuring it in plasma, which shows a similar time course of concentration changes as nicotine, indicating the specificity of exposure to this carcinogen in tobacco users.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Chemistry, Medicinal
Sebastian Schmidtsdorff, Jonas Neumann, Alexander H. H. Schmidt, Maria K. K. Parr
Summary: A total of 249 drug samples (substances, tablets, capsules, etc.) from the European pharmaceutical market collected since 2019 were analyzed for 16 nitrosamines (NAs). NAs were detected in 2.0% of the cases, including known contaminants such as NDMA and NDSRI in drugs like losartan, valsartan, metformin, and ranitidine. A new finding revealed the contamination of a molsidomine tablet sample with NMor, exceeding the toxicological allowable intake. Although regulatory investigations have focused on nitrosamines recently, the list of affected products continues to grow, emphasizing the importance of nitrosamine screenings.
ARCHIV DER PHARMAZIE
(2023)
Article
Chemistry, Analytical
Jonas Neumann, Sebastian Schmidtsdorff, Alexander H. Schmidt, Maria K. Parr
Summary: Currently, there is limited information on the use of ternary eluent compositions in supercritical fluid chromatography for peptide separation. This study investigates the benefits of adding acetonitrile to methanol as a modifier, increasing the resolution of peptide fingerprints and altering the elution order of analytes. A generic method development protocol for polypeptides is proposed based on the optimized conditions for three cyclic antibiotic peptides.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Food Science & Technology
Tasha Yuliandra, Konstantina Touvleliou, Xavier de la Torre, Francesco Botre, Steffen Loke, Eduard Isenmann, Sarah Valder, Patrick Diel, Maria Kristina Parr
Summary: This study investigates the urinary elimination of ecdysterone and its metabolites in humans after consuming two different culinary preparations of spinach. The results show that ecdysterone and its metabolites can be detected in urine after consuming both sauteed spinach and spinach smoothie. However, only a small proportion of ecdysterone from spinach is excreted into urine, indicating a low excretion rate of ecdysterone from spinach.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Biochemical Research Methods
Patrizia Leogrande, Daniel Jartdines, Dayamin Martinez-Brito, Xavier de la Torre, Maria Kristina Parr, Francesco Botre
Summary: A metabolomic workflow based on high-resolution mass spectrometry and computational tools is proposed as an alternative strategy for metabolite detection and identification. This approach allows for investigation of chemically different compounds, maximizing the information obtainable from the data while minimizing resources required.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Lukas C. Harps, Daniel A. Bizjak, Ulrich Girreser, Martina Zuegel, Juergen M. Steinacker, Patrick Diel, Maria Kristina Parr
Summary: In this study, the quantitation of formoterol, salbutamol, and its phase II metabolite salbutamol-4'-O-sulfate in urine and serum samples using ultra-high performance liquid chromatography-tandem mass spectrometry was successfully demonstrated. The highest concentrations of formoterol, salbutamol, and salbutamol-4'-O-sulfate in serum were found to be 14.2 pg/mL, 10.0 ng/mL, and 21.4 ng/mL, respectively, while in urine (after deglucuronidation), the maximum concentrations were 17.2 ng/mL, 948.5 ng/mL, and 2738.5 ng/mL, respectively. Sex-specific differences were also observed in serum concentrations and urinary excretion. These results highlight the importance of including and elucidating phase II metabolites in quantitation methods for antidoping.
Article
Chemistry, Analytical
Yan Felix Karl Dyck, Daniel Rehm, Karsten Winkler, Volker Sandig, Wolfgang Jabs, Maria Kristina Parr
Summary: The use of forced degradation to study the modifications of monoclonal antibodies was examined. It was found that the middle-up approach yielded similar results to the more expensive bottom-up approach in a forced oxidation biosimilar comparability study. This suggests that middle-up analysis can play a crucial role in biosimilar studies, but confirmation of modifications should be done using bottom-up analysis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Lukas Corbinian Harps, Annika Lisa Jendretzki, Clemens Alexander Wolf, Ulrich Girreser, Gerhard Wolber, Maria Kristina Parr
Summary: The aim of this study was to develop and optimize a chiral HPLC-MS/MS method for quantitative analysis of (R)-/(S)-salbutamol and (R)-/(S)-salbutamol-4'-O-sulfate in human urine to investigate the stereoselectivity in the sulfonation pathway of human phase II metabolism. The method development included systematic screening of columns and mobile phases, optimization of electrospray ionization settings, and comparison of matrix-assisted and predicted values. A longitudinal excretion study in one healthy volunteer revealed a strong preference for sulfonation of (R)-salbutamol in in vivo experiments. In vitro and in silico investigations confirmed these findings.
Article
Chemistry, Analytical
Annika Lisa Jendretzki, Lukas Corbinian Harps, Yanan Sun, Felix Bredendiek, Matthias Bureik, Ulrich Girreser, Xavier de la Torre, Francesco Botre, Maria Kristina Parr
Summary: The aim of the study was to quantitatively determine salbutamol and its sulfoconjugated metabolite in urine samples using achiral UHPLC-MS/MS. A reference material of salbutamol-4'-O-sulfate was biosynthesized and characterized. The study found that the discrimination of racemic salbutamol and levosalbutamol could be achieved by evaluating the proportion of salbutamol-4'-O-sulfate to salbutamol.
Article
Biochemistry & Molecular Biology
Ginevra Giacomello, Carolin Otto, Josef Priller, Klemens Ruprecht, Chotima Boettcher, Maria Kristina Parr
Summary: Neuroinflammation is a common characteristic of neurological diseases, such as multiple sclerosis (MScl) and Alzheimer's disease (AD), involving inflammatory responses in the CNS. The article investigates the metabolic changes in blood monocytes after exposure to CSF of healthy individuals or patients with AD or MScl. The findings suggest significant alterations in monocyte metabolism, indicating a metabolic reprogramming possibly related to the change of compartment and/or disease-related factors.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fan Yang, Maxi Wenzel, Matthias Bureik, Maria Kristina Parr
Summary: Propranolol, a non-selective beta-blocker, has been used in the treatment of cardiovascular diseases. This study identified glucuronic metabolites derived from hydroxypropranolol in human urine and found several UGT enzymes involved in the glucuronidation of hydroxypropranolol. The analysis of metabolites and urine samples indicates that glucuronidation preferentially occurs at the aromatic hydroxy groups under physiological conditions.
Article
Chemistry, Analytical
Katerina Plachka, Felix Bredendiek, Lucie Novakova, Maria Kristina Parr
Summary: This study developed effective methods for metal ion analysis using CO2-based mobile phase. The methods offer a fast and green alternative for metal ion analysis, as they do not require laborious synthesis and isolation of solid metal complexes. Furthermore, the methods were shown to be applicable for the determination of metal-EDTA complexes of various metals.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Fan Yang, Sangeeta Shrestha Sharma, Matthias Bureik, Maria Kristina Parr
Summary: This study investigates the functional interactions between human CYP2D6 and four human UGTs. The co-expression of UGT1A7, UGT1A8, or UGT1A9 increases the activity of CYP2D6, while UGT2A1 co-expression has no effect. Conversely, the co-expression of CYP2D6 suppresses the activities of all four UGTs. These findings suggest that CYP-UGT interactions can affect drug metabolism and contribute to adverse drug reactions and changes in drug efficacy.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)