Article
Reproductive Biology
Marta Neila-Montero, Marta F. Riesco, Rafael Montes-Garrido, Cristina Palacin-Martinez, Cesar Chamorro, Paulino de Paz, Mercedes Alvarez, Luis Anel, Luis Anel-Lopez
Summary: The study aimed to define the optimal conditions for centrifugation of ram semen in order to ensure the unaffected fertilization ability of the sperm. The results showed that centrifugation at 1200xg for 10 minutes using INRA 96 extender, slow refrigeration, and room temperature achieved high recovery rates and good quality sperm without detrimental effects on fertility.
Article
Biochemical Research Methods
Jess E. Mulhall, Natalie A. Trigg, Ilana R. Bernstein, Amanda L. Anderson, Heather C. Murray, Petra Sipila, Tessa Lord, John E. Schjenken, Brett Nixon, David A. Skerrett-Byrne
Summary: The testes and vas deferens are separated by the epididymis, a tubule with a unique microenvironment that is crucial for sperm maturation. The epididymal epithelium creates this microenvironment through the release of extracellular vesicles and small non-coding RNAs. In this study, the authors used mass spectrometry to compare the protein profiles of an immortalized mouse epididymal epithelial cell line to native mouse epididymal epithelial cells. They found that many proteins identified in the cell line were also present in native cells, supporting the use of this cell line as an in vitro model for studying epididymal epithelial cell function.
Article
Biology
Muhammad Tayyab Khan, Muhammad Irfan-ur-Rehman Khan, Ejaz Ahmad, Muhammad Rizwan Yousaf, Muhammad Oneeb
Summary: This study found that adding extracellular ATP and quercetin to ram semen can improve the quality of post-thawed semen and its fertilization potential. The combination of 1 mM ATP and 20 μM QUE or 1 mM ATP and 10 μM QUE showed positive effects on sperm motility and membrane integrity. Additionally, using the combination of 1 mM ATP and 10 μM QUE in the extender significantly improved the in vivo fertility of Lohi rams.
Article
Cell Biology
Abdullah Kaya, Sule Dogan, Peter Vargovic, Naseer Ahmad Kutchy, Pablo Ross, Einko Topper, Richard Oko, Frans van der Hoorn, Peter Sutovsky, Erdogan Memili
Summary: This study identified sperm proteins ODF2 and PAWP as biomarkers for assessing semen quality and predicting sire fertility, providing valuable insights for improving livestock reproductive performance in the cattle industry.
CELL AND TISSUE RESEARCH
(2022)
Article
Veterinary Sciences
Belen Pequeno, Cristina Castano, Manuel Alvarez-Rodriguez, Paula Boveda, Maria Gemma Millan de la Blanca, Adolfo Toledano-Diaz, Diego Andres Galarza, Heriberto Rodriguez-Martinez, Belen Martinez-Madrid, Julian Santiago-Moreno
Summary: The study aimed to investigate the influence of osmotic changes on the location of aquaporins (AQPs) in spermatozoa during freeze-thawing process. Individual variability in terms of freezeability was observed among rams and their ejaculates. AQP3 expression in the mid- and principal piece of spermatozoa was found to be higher in frozen-thawed samples compared to fresh specimens in the good freezability group, but such differences were not detected in the poor freezability group. AQP3 expression is associated with sperm cryotolerance and can be used as a biomarker.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Biology
Madeleine Van de Hoek, Jessica P. Rickard, Simon P. de Graaf
Summary: This review provides an overview of the mechanical and energetic processes involved in mammalian sperm motility and the impact of various factors on the motility of ram sperm. It also explores the current methods used in the sheep livestock production industry for assessing ram semen quality and predicting artificial insemination success. These methods include mass motility assessment, subjective motility evaluation, computer-assisted sperm analysis, as well as newly developed methods such as flagellar tracing and molecular assays. The understanding and assessment of sperm motility are crucial for optimizing ram semen processing and quality control in ovine artificial breeding programs.
Article
Agriculture, Dairy & Animal Science
Jakub Vozaf, Andrea Svoradova, Andrej Balazi, Jaromir Vasicek, Lucia Olexikova, Linda Dujickova, Alexander Makarevich, Rastislav Jurcik, Hana Duranova, Peter Chrenek
Summary: Biodiversity protection is a vital challenge, and cryopreservation of sperm is an effective tool for animal genetic resource conservation. This study compares the quality parameters of cryopreserved sperm among three different Slovak sheep breeds. The study finds that the selected cryopreservation protocol is suitable for all three breeds and can greatly benefit biodiversity protection.
Article
Agriculture, Dairy & Animal Science
Albert Salas-Huetos, Jordi Ribas-Maynou, Yentel Mateo-Otero, Carolina Tamargo, Marc Llavanera, Marc Yeste
Summary: This study investigates the expression and function of miRNA in bovine sperm. The results show that 8 out of 10 candidate miRNAs are consistently detected in bovine sperm, with one miRNA (bta-miR-138) being significantly lower in the subfertility group compared to the high fertility group. Additionally, three other miRNAs (bta-miR-19b, bta-miR-26a, and bta-miR-7) are correlated with sperm function.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Obstetrics & Gynecology
Elisabetta Baldi, Meurig T. Gallagher, Stepan Krasnyak, Jackson Kirkman-Brown
Summary: The sixth edition of the World Health Organization manual explores extended examination methods that offer key diagnostics for investigating male reproductive system function. These methods go beyond basic semen analysis and can specifically guide the clinical characterization of fertile or infertile men. Multiparametric scoring for sperm morphological defects, sperm DNA fragmentation, and computer-assisted analysis of sperm or semen are among the most widely used and debated techniques.
FERTILITY AND STERILITY
(2022)
Article
Veterinary Sciences
Noelia Mendoza, Adriana Casao, Juan Domingo, Francisco Quintin, Adolfo Lavina, Enrique Fantova, Jose Alvaro Cebrian-Perez, Teresa Muino-Blanco, Rosaura Perez-Pe
Summary: This study explored the impact of non-traditional sperm quality markers in seminal doses on fertilizing ability, identifying intact membrane, non-capacitated spermatozoa, and DNA-intact spermatozoa as potential markers to discard males in AI programs. This is crucial for achieving satisfactory fertility rates from an economic point of view.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Biochemical Research Methods
Mika Alexia Miyazaki, Raquel Lozano Guilharducci, Paula Intasqui, Ricardo Pimenta Bertolla
Summary: This review summarizes recent advances in the study of sperm proteomics and its impact on sperm structure, function, and fertility. A literature search was conducted to summarize the latest findings in sperm proteomics. The authors propose that understanding the sperm proteome can help identify crucial pathways affecting fertility and potentially unravel the mechanisms involved in idiopathic infertility.
EXPERT REVIEW OF PROTEOMICS
(2023)
Article
Biology
Patricia Peris-Frau, Irene Sanchez-Ajofrin, Alicia Martin Maestro, Carolina Maside, Daniela Alejandra Medina-Chavez, Olga Garcia-alvarez, Maria del Rocio Fernandez-Santos, Vidal Montoro, Jose Julian Garde, Manuel Ramon, Ana Josefa Soler
Summary: Spermatozoa go through diverse changes to achieve fertilizing potential and specific motility pattern. Cryopreservation alters the distribution of motile sperm subpopulations, affecting sperm quality. Changes in sperm motility and tyrosine phosphorylation key events during capacitation can be modified by the cryopreservation process.
Article
Reproductive Biology
Jones Ofosu, Moses Addo Nartey, Xianhong Mo, Jiangfeng Ye, Yan Zhang, Changjun Zeng, Ming Zhang, Yi Fang, Guangbin Zhou
Summary: Metabolites in ram sperm play important roles in maintaining plasma membrane integrity, energy metabolism, and cryotolerance regulation. This study identified differential metabolites during cryopreservation in ram sperm and found that certain key polyunsaturated fatty acids were down-regulated during cooling and cryopreservation. The differentially regulated metabolites were enriched in several metabolic pathways, providing new knowledge to improve the cryopreservation process.
Article
Cell Biology
Tomer Avidor-Reiss, Luke Achinger, Rustem Uzbekov
Summary: Centrioles are subcellular organelles that are crucial for normal cell function and development. They are found in most animal cell types but are absent in egg cells and exhibit diverse characteristics in sperm cells. The precise role of centrioles in fertility and early embryo development remains unclear.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Anais Vitorino Carvalho, Laura Soler, Aurore Thelie, Isabelle Grasseau, Luiz Cordeiro, Daniel Tomas, Ana-Paula Teixeira-Gomes, Valerie Labas, Elisabeth Blesblois
Summary: Using two complementary proteomic methods, this study identified 57 differentially abundant proteins in fertile and subfertile rooster sperm. These proteins are involved in various molecular pathways such as flagellum integrity and movement, mitochondrial functions, sperm maturation, storage in female tract, and oocyte-sperm interaction. The data provides new insights into the complexity of male fertility in chickens and suggests the use of a combination of markers for predicting male fertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Andrology
Mustafa Numan Bucak, Nazan Keskin, Mustafa Bodu, Bulent Bulbul, Mesut Kirbas, Ali Erdem Ozturk, Fateme Frootan, Pinar Ili, Huseyin Ozkan, Nuri Baspinar, Sukru Dursun
Summary: The study aimed to establish a suitable ram semen extender model by examining different combinations of glycerol concentrations, trehalose, and boron to prevent sperm deterioration and investigate gene expressions. Results showed that supplementation of 0.25 mm boron improved sperm parameters and acrosome integrity, while high boron concentrations had adverse effects on sperm parameters and antioxidant gene expression after thawing. The use of sufficient boron can decrease cryodamages during sperm cryopreservation and tissue engineering.
Article
Food Science & Technology
Ozden Ozgun-Acar, Gurbet Celik-Turgut, Huseyin Guner, Serdar Sezer, Alaattin Sen
Summary: This study evaluated the biochemical, pharmacological, and toxicological aspects of Capparis ovata water extract (COWE). The results showed that COWE is rich in minerals, unsaturated fatty acids, and flavonoids/phenolics, and has high radical-scavenging ability. It significantly inhibits inflammatory responses and modulates immune responses through gene expression regulation.
FOOD SCIENCE & NUTRITION
(2022)
Article
Plant Sciences
Halil Senol, Ozden Ozgun-Acar, Aydan Dag, Ahmet Eken, Huseyin Guner, Zaliha Gamze Aykut, Gulacti Topcu, Alaattin Sen
Summary: In this study, the therapeutic effect of a compound called OPCA on multiple sclerosis (MS) was investigated using a preclinical model. The results demonstrated that OPCA protected the mouse brain, maintained blood-brain barrier integrity, and reduced inflammation. Furthermore, OPCA increased the expression of genes involved in myelin development and structure, and reduced the release of pro-inflammatory cytokines. Therefore, OPCA is a promising and appealing candidate for treating human MS.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Omer Faruk Karasor, Mustafa Numan Bucak, Mihai Cenariu, Mustafa Bodu, Mehmet Taspinar, Filiz Taspinar
Summary: In this study, the effects of ROCK inhibitor Y-27632, antifreeze protein III, and boron on the spermatological parameters of Ankara buck semen after freeze-thawing were investigated. The results showed that the additives improved post-thaw motility and DNA damage of the semen.
Article
Immunology
Mustafa Burak Acar, Serife Ayaz-Guner, Huseyin Guner, Gokcen Dinc, Aysegul Ulu Kilic, Mehmet Doganay, Servet Ozcan
Summary: We aimed to identify immunodominant vaccine candidate proteins against antibiotic-resistant A. baumannii by capturing outer-membrane and secreted proteins using patient IgGs and LC-MS/MS analysis. We identified several notable vaccine candidates that triggered a de facto immune response.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biodiversity Conservation
Hande Mumcu, Emine Tugce Sarac Cebeci, Meryem Menekse Kilic, Anil Cebeci, Yagmur Gunes, Ilker Karacan, Merve Oztug, Nurgul Balci, Nevin Gul Karaguler
Summary: In this study, a novel strain of Pseudomonas mandelii, named KGI_MA19, was identified and characterized from sediment samples of King George Island in Antarctica. This psychrotolerant and halotolerant strain exhibited the ability to use disaccharides as a carbon source, denitrification ability, and multiple antibiotic resistance. Comparative proteomic analyses revealed the adaptive mechanisms of P. mandelii KGI_MA19 in the cold environment.
Article
Agriculture, Dairy & Animal Science
Hasan Alkan, Fatma Satilmis, Mehmet Akif Demirel, Mustafa Bodu, Omer Faruk Yesilkaya, Muhammed Furkan Ciftci, Huseyin Erdem, Mustafa Agah Tekindal, Kubra Karakas Alkan
Summary: This study determined the effect of microfluidic sperm sorting chip on embryo development and quality in the sperm treatment step in in vitro embryo production in cattle. The study found that using the microfluidic sperm sorting chip increased the rate of reaching the blastocyst, embryo development, and quality in the cattle. It also reduced the possibility of apoptosis in developing blastocysts.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Agriculture, Dairy & Animal Science
Mehmet Bozkurt Ataman, Mehmet Akoz, Nurcan Donmez, Mustafa Bodu, Mustafa Kul, Mustafa Numan Bucak
Summary: The study aimed to investigate the effects of vitamin A, vitamin E, and combined beta-carotene plus vitamin E injections on fertility parameters in ewes. The results showed that the combined administration of vitamin A and vitamin E can increase the multiple birth rate and litter size.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Esra Bicici, Fatma Satilmis, Mustafa Bodu, Mehmet Akif Demirel, Kubra Karakas Alkan, Hasan Alkan
Summary: This study investigated the effect of putrescine supplementation in the maturation medium during in vitro embryo production in cattle. The results showed that putrescine supplementation increased the degree of cumulus expansion, the rate of resumption of meiosis, and the rate of reaching the blastocyst stage in oocytes. The addition of putrescine also led to better cell development in embryos.
ANIMAL BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Meltem Asicioglu, Merve Oztug, Nevin Gul Karaguler
Summary: This study aimed to develop a method traceable to the International System of Units for accurately measuring cardiac cTnI levels in human serum. The method involved immobilizing anti-cTnI antibodies onto magnetic nanoparticles, isolating cTnI from serum, and quantifying it using isotope dilution liquid chromatography-tandem mass spectrometry. The developed method showed high analytical performance in accurately quantifying cTnI and has the potential to harmonize cTnI results between clinical laboratories.
CLINICAL PROTEOMICS
(2023)
Article
Immunology
Sinem Gunalp, Derya Goksu Helvaci, Aysenur Oner, Ahmet Bursali, Alessandra Conforte, Hueseyin Guener, Gokhan Karakulah, Eva Szegezdi, Duygu Sag
Summary: This study investigates the role of TNF-related apoptosis-inducing ligand (TRAIL) in macrophage polarization. It shows that TRAIL promotes the polarization of human macrophages towards a proinflammatory M1 phenotype via both DR4 and DR5, suggesting that targeting DRs can enhance the anti-tumorigenic response of macrophages in the tumor microenvironment by increasing M1 polarization.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Veterinary Sciences
Durmus Hatipoglu, Mehmet Burak Ates, Mustafa Bodu
Summary: This study investigated the protective effect of Nigella sativa oil against testicular damage induced by bisphenol A. The results showed that Nigella sativa oil reduced LH levels, improved sperm motility and mitochondrial activity, and alleviated testicular inflammation, demonstrating its protective effect.
MEDYCYNA WETERYNARYJNA-VETERINARY MEDICINE-SCIENCE AND PRACTICE
(2023)
Article
Biochemical Research Methods
Serife Ayaz-Guner, Mustafa Burak Acar, Dudu Boyvat, Huseyin Guner, Habibe Bozalan, Melis Guzel, Selin Kubra Yildir, Nilay Altinsoy, Fatma Findik, Musa Karakukcu, Servet Ozcan
Summary: This article presents a protocol combining MACS and surface biotinylation for isolating target cell subsets from human PBMCs and analyzing their biotinylated proteins. The protocol allows in-column surface biotinylation of specific cell subsets with minimal membrane disruption.
Meeting Abstract
Medical Laboratory Technology
C. Swart, M. Weller, V. Delatour, M. Quaglia, M. Oztug, A. Gallo, H. Schwalbe, C. Cobbaert, A. Reid, A. Kessler, G. Nordin
CLINICA CHIMICA ACTA
(2022)