Article
Microbiology
Alireza Barani, Akram Sadat Tabatabaee Bafroee, Leila Jabalameli
Summary: This study in Ahvaz, Iran found a high prevalence of ESBL genes among intestinal E. coli isolates from drug users, with bla-TEM gene being the most prevalent. The multidrug resistance (MDR) patterns, especially simultaneous resistance to seven antimicrobials, highlight the risk of transferring resistance genes between nonpathogenic and pathogenic bacteria.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Immunology
Tam Tran, Sylvia Checkley, Niamh Caffrey, Chunu Mainali, Sheryl Gow, Agnes Agunos, Karen Liljebjelke
Summary: This study identified potential horizontal gene transfer events of antimicrobial resistance genes between E. coli and Salmonella in broiler chicken production, revealing various resistant genes in the process.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Environmental Sciences
Murugadas Vaiyapuri, Anna SherinPulithara Sebastian, Iris George, Sandhya Soolamkandath Variem, Radhakrishnan Nair Vasudevan, Joshy Chalil George, Madhusudana Rao Badireddy, Visnuvinayagam Sivam, Shaheer Peeralil, Devi Sanjeev, Muthulakshmi Thandapani, Sheela Albert Moses, Ravishankar Chandragiri Nagarajarao, Mukteswar Prasad Mothadaka
Summary: The study assessed the burden of antimicrobial resistance (AMR) in Escherichia coli in Vembanad Lake, Kerala, India. The findings revealed high prevalence of E. coli with resistance to multiple antibiotics, presence of genetically diverse ESBL types, and potential for resistance transmission through plasmids. This highlights the health hazards posed by genetically diverse ESBL E. coli in the lake and the need for pragmatic control measures.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Omar E. Ahmed, Atif Asghar, Majid Bamaga, Fayez Bahwerth, Mutasim Ibrahim
Summary: The study aimed to characterize aminoglycoside resistance genes in multidrug-resistant (MDR) Klebsiella pneumoniae collected during the COVID-19 pandemic. Out of 89 K. pneumoniae isolates, 57.3% exhibited MDR patterns and showed elevated resistance to aminoglycoside agents. PCR assays detected aminoglycoside genes in 82.3% of the MDR strains, with the rmtD gene being the most predominant.
Article
Infectious Diseases
Yue Li, Yiwei Zhu, Wanqing Zhou, Zhongju Chen, Robert A. Moran, Huanhuan Ke, Yu Feng, Willem van Schaik, Han Shen, Jingshu Ji, Zhi Ruan, Xiaoting Hua, Yunsong Yu
Summary: This study characterized AFM-2 and AFM-3 metallo-beta-lactamase from clinical Pseudomonas aeruginosa isolates. The results showed that these enzymes exhibited beta-lactamase activity comparable to NDM-1, and the co-carriage of bla(AFM) and bla(KPC) genes rendered P. aeruginosa resistant to antipseudomonal beta-lactams. Furthermore, the association of bla(AFM) genes with translocatable genetic elements and plasmids highlighted their potential for dissemination.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Environmental Sciences
Adriana Trotta, Margie Cirilli, Mariarosaria Marinaro, Suncica Bosak, Georgia Diakoudi, Stefano Ciccarelli, Serena Paci, Domenico Buonavoglia, Marialaura Corrente
Summary: Sea turtles are useful indicators for monitoring antimicrobial resistance in marine ecosystems. A study in the Mediterranean Sea found a high prevalence of multidrug-resistant Gram negative bacteria in wounded turtles, with some bacteria carrying AmpC beta-lactamase and extended-spectrum beta-lactamase genes.
MARINE POLLUTION BULLETIN
(2021)
Article
Immunology
Eun Jin Kim, Jiwon Lee, Youngbae Yoon, Donghyun Lee, Yeongjun Baek, Chika Takano, Jun Sakai, Takahiro Iijima, Dai Kanamori, Humphrey Gardner, Robert E. McLaughlin, Paul E. Kilgore, Akihiro Nakamura, Takashi Ogihara, Satoshi Hayakawa, Tomonori Hoshino, Dong Wook Kim, Mitsuko Seki
Summary: Rapid evaluation of antimicrobial susceptibility is crucial in treating nosocomial infections caused by Gram-negative bacteria with carbapenemases and metallo-beta-lactamases. We developed loop-mediated isothermal amplification (LAMP)-based assays for four beta-lactamase genes and successfully detected them in various clinical specimens using LAMP. Compared to conventional PCR, LAMP exhibited higher sensitivity in detecting low copy numbers of the target genes in DNA-spiked clinical specimens.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Ji-Hyun Choi, Md. Sekendar Ali, Bo-Youn Moon, Hee-Young Kang, Su-Jeong Kim, Hyun-Ju Song, Abraham Fikru Mechesso, Dong-Chan Moon, Suk-Kyung Lim
Summary: A total of 836 Escherichia coli isolates were recovered from dogs and cats between 2018 and 2019, with 17.1% and 21.2% of isolates resistant to cefovecin and enrofloxacin, respectively. Higher resistance rates were observed in dog isolates compared to cat isolates. The detection of the ST131 clone carrying bla(CTX-M-14/15) in companion animals poses a public health threat.
Article
Infectious Diseases
Hasan Ejaz, Muhammad Usman Qamar, Kashaf Junaid, Sonia Younas, Zeeshan Taj, Syed Nasir Abbas Bukhari, Abualgasim E. Abdalla, Khalid O. A. Abosalif, Naveed Ahmad, Zikria Saleem, Eman H. M. Salem
Summary: The emergence of carbapenem-resistant Acinetobacter calcoaceticus-baumannii complex (CRACB) in clinical environments is a significant global concern. These critical pathogens have shown resistance to a broad spectrum of antibacterial drugs, including carbapenems. CRACB isolates displayed resistance to most antibiotics, except tigecycline and colistin.
Article
Veterinary Sciences
Hayyun Durrotul Faridah, Freshindy Marissa Wibisono, Freshinta Jellia Wibisono, Nabilatun Nisa, Fatimah Fatimah, Mustofa Helmi Effendi, Emmanuel Nnabuike Ugbo, Aswin Rafif Khairullah, Shendy Canadya Kurniawan, Otto Sahat Martua Silaen
Summary: This study aimed to determine the prevalence of ESBL-producing E. coli in broiler chickens in Indonesia. The results showed that 86.1% of the isolated E. coli were resistant to beta-lactam antibiotics, with 94.1% carrying the bla(CTX-M) gene and 38.2% carrying the bla(TEM) gene. The findings suggest that broiler chickens may serve as a potential reservoir of ESBL-producing E. coli, posing a threat to human health. Public health education is recommended to mitigate this potential risk.
JOURNAL OF VETERINARY RESEARCH
(2023)
Article
Pharmacology & Pharmacy
Khanzad Khudhur Jarjees, Rozhhalat Khudhur Jarjees, G. M. Qader
Summary: Pseudomonas aeruginosa is a common opportunistic pathogen with drug resistance potentially related to the production of broad spectrum beta lactamase enzymes. This study aimed to detect drug resistant strains and determine the frequency of extended spectrum beta lactamase enzymes in Pseudomonas aeruginosa. The findings highlight the increasing prevalence of multidrug resistant Pseudomonas aeruginosa strains with potential clinical implications.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Infectious Diseases
Riju Maharjan, Anup Bastola, Nabaraj Adhikari, Komal Raj Rijal, Megha Raj Banjara, Prakash Ghimire, Upendra Thapa Shrestha
Summary: This study identified bacterial co-infections in the lower respiratory tract and detected plasmid-mediated bla(TEM) and bla(CTX-M) genes among Extended-Spectrum beta-Lactamase (ESBL) producing isolates in people living with HIV/AIDS.
BMC INFECTIOUS DISEASES
(2022)
Article
Microbiology
Neda Yousefi Nojookambari, Mehrzad Sadredinamin, Razieh Dehbanipour, Zohreh Ghalavand, Gita Eslami, Maryam Vaezjalali, Bahram Nikmanesh, Sajjad Yazdansetad
Summary: The study examined the antibiotic resistance, prevalence of beta-lactamase-encoding genes, and clonal relationships of Acinetobacter baumannii strains isolated from pediatric patients in Tehran, Iran. The results showed high rates of multi-drug resistant and extensively drug-resistant isolates, with certain beta-lactamase genes being prevalent. The presence of well-established clones poses a potential therapeutic challenge due to genetic elements associated with resistance.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Review
Medicine, General & Internal
Rakhi Issrani, Jagat Reddy, Tarek H. El-Metwally Dabah, Namdeo Prabhu, Mohammed Katib Alruwaili, Manay Srinivas Munisekhar, Sultan Meteb Alshammari, Shmoukh Fahad Alghumaiz
Summary: Oral health and systemic health are interdependent, with periodontal diseases being multifactorial and mainly involving bacterial infections. Studying oral microbial flora is crucial for the early diagnosis and treatment of systemic diseases.
Article
Medicine, Research & Experimental
Mutasim E. Ibrahim, Tarig B. Algak, Mohammed Abbas, Bahaeldin K. Elamin
Summary: The study revealed a diversity of ESBL genes in Gram-negative bacterial isolates, with bla(TEM) being the most prevalent type. The emergence of various ESBL genes with several co-existing genotypes is alarming, rendering extensive surveillance studies necessary to understand the transmission and epidemiology of such resistant gene-carrying isolates.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)