Review
Engineering, Biomedical
X. B. Chen, A. Fazel Anvari-Yazdi, X. Duan, A. Zimmerling, R. Gharraei, N. K. Sharma, S. Sweilem, L. Ning
Summary: Bioinks combine biomaterials and living cells, and are used in extrusion bioprinting to create 3D structures that resemble human tissue or organs. These printed constructs have various applications in tissue engineering and in vitro tissue modeling. The properties of the bioinks, including rheological, mechanical, and biological properties, as well as the printing process, play a crucial role in successful printing and application. This article critically reviews the latest developments in bioinks and biomaterial solutions for extrusion bioprinting, emphasizing bioink synthesis, characterization, and their influence on the printing process.
BIOACTIVE MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Mohamadmahdi Samandari, Jacob Quint, Alejandra Rodriguez-delaRosa, Indranil Sinha, Olivier Pourquie, Ali Tamayol
Summary: Skeletal muscle injuries can lead to mobility limitations and loss of independence, with current treatments showing variable effectiveness. Skeletal muscle tissue engineering and bioprinting are considered promising solutions for reconstructing complex muscle structures and improving functional recovery.
ADVANCED MATERIALS
(2022)
Review
Biochemistry & Molecular Biology
Jianhua Zhang, Esther Wehrle, Marina Rubert, Ralph Mueller
Summary: Three-dimensional (3D) bioprinting is a promising approach for tissue engineering, allowing precise placement of cells and biomaterials to mimic complex microstructures of biological tissues. Bioprinted tissues can benefit from exposure to fluid mechanical cues provided by bioreactor systems, aiding in tissue development and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Martina M. De Santis, Hani N. Alsafadi, Sinem Tas, Deniz A. Bolukbas, Sujeethkumar Prithiviraj, Iran A. N. Da Silva, Margareta Mittendorfer, Chiharu Ota, John Stegmayr, Fatima Daoud, Melanie Koenigshoff, Karl Sward, Jeffery A. Wood, Manlio Tassieri, Paul E. Bourgine, Sandra Lindstedt, Sofie Mohlin, Darcy E. Wagner
Summary: The study introduces a hybrid bioink composed of alginate and rECM for 3D bioprinting, showing promising printing properties and biological inductive capabilities for generating human tissue.
ADVANCED MATERIALS
(2021)
Review
Biotechnology & Applied Microbiology
Haonan Wang, Huaqing Yu, Xia Zhou, Jilong Zhang, Hongrui Zhou, Haitong Hao, Lina Ding, Huiying Li, Yanru Gu, Junchi Ma, Jianfeng Qiu, Depeng Ma
Summary: This article reviews the significance and recent progress of ECM-based biomaterials in 3D bioprinting, as well as their applications in tissue regeneration and cancer research. There is great potential for future development.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Zhiyuan Kong, Xiaohong Wang
Summary: Large diameter artery defects can be substituted by unbiodegradable polymers, while small diameter blood vessels and microvessels pose challenges in constructing complex vascular models. Vascularization strategies can be categorized into self-generated capillaries from implantation, pre-constructed vascular channels, and 3D printed cell-laden hydrogels. This review discusses the latest advances in 3D bioprinting of vascularized tissues/organs, including new printing techniques and bioinks for promoting angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Matthew L. Bedell, Angelica L. Torres, Katie J. Hogan, Ziwen Wang, Bonnie Wang, Anthony J. Melchiorri, K. Jane Grande-Allen, Antonios G. Mikos
Summary: The investigation of novel hydrogel systems in osteochondral tissue engineering is important for understanding the relationship between biomaterials, cells, and other factors. This study explored the use of 3D printing to fabricate hydrogel environments that mimic tissue-specific architectures. The researchers characterized a methacryloylated gelatin composite hydrogel system and investigated the effects of additives on bone and cartilage-like matrix deposition and genetic markers in human mesenchymal stem cells (hMSCs). New assays were developed to study the effects of different bioinks and printing techniques, and viable constructs with encapsulated hMSCs were successfully printed. The results demonstrate the potential of these novel hydrogel formulations for promoting osteochondral phenotypes and highlight the importance of bioink additives in printability.
Article
Engineering, Biomedical
Sara Cristina Pedroza-Gonzalez, Marisela Rodriguez-Salvador, Baruc Emet Perez-Benitez, Mario Moises Alvarez, Grissel Trujillo-de Santiago
Summary: This scientometric analysis examines the development and use of bioinks for 3D bioprinting in the period from January 2000 to June 2019. The main considerations for bioink applications include cost, availability, practicality, and basic biological factors. Extrusion bioprinting is currently the most popular technique, with bioinks commonly used for generic characterization and cartilage bioprinting applications. Despite advancements in functional bioinks, the field is still in early stages with alginate and gelatin methacryloyl being the most commonly used hydrogels.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2021)
Review
Materials Science, Multidisciplinary
Haonan Wang, Shihao Bi, Bingbing Shi, Junchi Ma, Xiangwei Lv, Jianfeng Qiu, Yunyun Wei
Summary: 3D bioprinting enables the controlled deposition of bioink containing cells to fabricate complex bionic tissue structures in a fast and scalable manner, revolutionizing clinical organ transplantation. Bioprinting has broad applications in tissue engineering, life sciences, and clinical medicine. This review explores the concept, development, and composition of bioink, with a focus on the advantages and disadvantages of various biomaterials, as well as the use of common cells and biomolecules. Additionally, it discusses the properties and applications of stimuli-responsive smart materials for 4D bioprinting and summarizes the challenges and development trends of bioink.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Zaman Ataie, Sina Kheirabadi, Jenna Wanjing Zhang, Alexander Kedzierski, Carter Petrosky, Rhea Jiang, Christian Vollberg, Amir Sheikhi
Summary: This study overcomes the limitations of traditional granular hydrogel bioinks in 3D bioprinting by using reversible interfacial nanoparticle self-assembly in the microgels, enabling the fabrication of bioinks with microporous structure. The microporous architecture promotes cell penetration and oxygen transfer, improves cell viability, and facilitates 3D cell seeding.
Review
Biochemistry & Molecular Biology
Fouad Al-Hakim Khalak, Fatima Garcia-Villen, Sandra Ruiz-Alonso, Jose Luis Pedraz, Laura Saenz-del-Burgo
Summary: In recent years, there has been a growing demand for improving the regeneration of damaged tendons. Current treatments using grafts have limitations as they cannot provide sufficient support for tissue regeneration. A recent study has highlighted the potential benefits of combining 3D bioprinting and dECM as a novel bioink biomaterial. However, there are still challenges to overcome, such as the poor structural integrity and slow gelation properties of dECM bioinks.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Qian Wang, Yang Liu, Shuqing Zhang, Fan He, Tong Shi, Jizong Li, Zhimin Wang, Jia Jia
Summary: Bioprinting is a promising technology for tissue engineering and regenerative medicine, but one of its limitations is the reproduction of the extracellular matrix (ECM) with sufficient bioactivities for bioprinted cells. Exosomes, natural biological particles, show great potential in supporting cells for bioprinting. This review summarizes the recent advances of exosome-based bioprinting in tissue engineering and regenerative medicine, including applications in bone tissue engineering, immunological regulations, angiogenesis, and neural regenerations.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Biochemistry & Molecular Biology
Xavier Barcelo, Kian F. Eichholz, Orquidea Garcia, Daniel J. Kelly
Summary: The study investigated the effect of alginate oxidation on the degradation rate of bioinks, resulting in a range of degradable bioinks with the degradation rate highly dependent on the content of partially oxidized alginate. Despite high mass loss, all bioink blends except the 0:100 group maintained the initially printed geometry throughout a 4 week in vitro culture period and supported robust chondrogenic differentiation of mesenchymal stem/stromal cells.
Article
Chemistry, Multidisciplinary
Hyeonji Kim, Byeongmin Kang, Xiaolin Cui, Se-Hwan Lee, Kwangseok Lee, Dong-Woo Cho, Woonbong Hwang, Tim B. F. Woodfield, Khoon S. Lim, Jinah Jang
Summary: The new light-activated dECM bioinks with ruthenium/sodium persulfate (dERS) introduced in this study have improved printing performance and mechanical properties, enabling the fabrication of complex constructs with increased shape fidelity, expanding the biofabrication possibilities.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Wonbin Park, Ge Gao, Dong-Woo Cho
Summary: This review discusses the advances in musculoskeletal tissue engineering using 3D bioprinting technology and decellularized extracellular matrix bioink. It focuses on in vivo regeneration and in vitro modeling of musculoskeletal tissue, while also addressing current breakthroughs, limitations, and future prospects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Editorial Material
Surgery
Dominik Promny, Jasmin Gruener, Theresa Hauck, Raymund E. Horch
HANDCHIRURGIE MIKROCHIRURGIE PLASTISCHE CHIRURGIE
(2023)
Article
Cell Biology
Dominik Steiner, Lea Reinhardt, Laura Fischer, Vanessa Popp, Carolin Koerner, Carol Geppert, Tobias Baeuerle, Raymund E. Horch, Andreas Arkudas
Summary: The microvascular endothelial network is crucial for osteogenesis and bone tissue engineering. Endothelial progenitor cells (EPCs) have a strong angiogenic potential and can enhance the vascularization and tissue formation of scaffolds. This study investigated the effects of EPCs on the vascularization and bone formation of a hydroxyapatite and beta-tricalcium phosphate-fibrin scaffold. The results showed successful scaffold vascularization in all groups, and the EPC/MSC coimplantation group demonstrated bone formation. The arteriovenous loop was found to be an effective method for vascularization independent of scaffold cellularization with EPCs.
Article
Cell Biology
Ravikumar Vaghela, Andreas Arkudas, Daniel Gage, Carolin Koerner, Stephan von Hoersten, Sahar Salehi, Raymund E. Horch, Maximilian Hessenauer
Summary: Due to limitations in current in vivo experimental designs, our understanding of vascular development and its implications for large-scale tissue engineering is limited. Thus, this study aimed to develop unique in vivo imaging chambers for live visualization of cellular processes in the arteriovenous (AV) loop model in rats. Two different types of chambers were developed and filled with modified gelatin hydrogel. Intravital microscopy (IVM) using fluorescein isothiocyanate (FITC)-labeled dextran and rhodamine 6G dye allowed visualization of leukocyte trafficking and examination of microvascular development in the AV loop for up to 21 days. The combination of these IVM chambers provides a comprehensive understanding of vascular development in the AV loop tissue engineering model.
Article
Health Care Sciences & Services
Justus Osterloh, Ingo Ludolph, Robert Gruetzmann, Alexander Meyer, Werner Lang, Raymund E. Horch, Katja Fechner, Andreas Arkudas
Summary: Soft-tissue sarcomas (STS) are rare malignancies occurring in various parts of the body, with the limbs being the most common site. Referral to a specialized sarcoma center is crucial for prompt and appropriate treatment. Interdisciplinary tumor boards should discuss STS treatment strategies, involving expertise from reconstructive surgeons for optimal outcomes. Evaluation of plastic reconstruction is necessary to avoid complications after extensive resection, and primary flap reconstruction may be preferable to secondary flap reconstruction for reducing complications.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Correction
Surgery
Markus Mulica, Raymund E. Horch, Andreas Arkudas, Aijia Cai, Wibke Mueller-Seubert, Theresa Hauck, Ingo Ludolph
FRONTIERS IN SURGERY
(2023)
Article
Surgery
N. Moellhoff, L. Prantl, B. Behr, J. P. Beier, A. Daigeler, A. Dragu, S. U. Eisenhardt, P. C. Fuchs, G. Germann, T. Hirsch, R. E. Horch, M. Infanger, R. Jakubietz, U. Kneser, S. Langer, M. Lehnhardt, H. G. Machens, P. Mailaender, B. Reichert, C. Radtke, D. J. Schaefer, F. Siemers, P. M. Vogt, H. Menke, R. E. Giunta
Summary: This research report presents the research performance of plastic surgeons in Germany for the period 2021/2022 and compares it with previous periods. The report shows that there has been an increase in the number of research applications and approvals in plastic surgery, and most participating centers have their own research infrastructure such as experimental laboratories and clinical trial centers.
HANDCHIRURGIE MIKROCHIRURGIE PLASTISCHE CHIRURGIE
(2023)
Article
Biochemistry & Molecular Biology
Maike Hermann, Ajay Peddi, Alexander Gerhards, Rafael Schmid, Deborah Schmitz, Andreas Arkudas, Volker Weisbach, Raymund E. Horch, Annika Kengelbach-Weigand
Summary: Chronic wounds are a global epidemic and regenerative medicine is exploring the use of adipose-derived stem cells (ADSC) for new therapies. This study investigated the use of platelet lysate (PL) as a substitute for foetal bovine serum (FBS) to create an ADSC secretome containing cytokines for optimal wound healing conditions. The ADSC secretome was tested on keratinocytes for migrational behavior and viability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Oncology
R. E. Horch, M. R. Kesting, S. Kersting, S. Fichtner-Feigl, A. Arkudas
FRONTIERS IN ONCOLOGY
(2023)
Editorial Material
Health Care Sciences & Services
Raymund E. Horch, Andreas Arkudas
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Celena A. Soergel, Aijia Cai, Rafael Schmid, Raymund E. Horch
Summary: Human skin is vulnerable to damaging influences, and severe skin defects often require surgical interventions. Bioprinting techniques offer an alternative approach by using biofabricated skin grafts to restore skin continuity and functions. Various bioprinting methods and strategies for graft vascularization can be employed to accelerate wound healing and reconstitute the physiological functions of the skin.
Article
Health Care Sciences & Services
Koblandy Khamitov, Wojciech Dudek, Andreas Arkudas, Mohamed Haj Khalaf, Chirag Parjiea, Mostafa Higaze, Raymund E. Horch, Horia Sirbu
Summary: In patients who underwent surgical resection for primary and secondary MCWTs, chest wall resections were found to be feasible with good perioperative outcomes. The overall survival rate was significantly higher in patients with primary MCWTs compared to those with secondary MCWTs.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Health Care Sciences & Services
Wibke Mueller-Seubert, Patrick Ostermaier, Raymund E. Horch, Luitpold Distel, Benjamin Frey, Ramona Erber, Andreas Arkudas
Summary: Different irradiation regimens have different effects on random-pattern flaps. A higher total dose of postoperative irradiation may increase flap necrosis, and irradiation can increase the expression of VEGF and HIF-1α.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Jana Schulik, Sahar Salehi, Aldo R. Boccaccini, Stefan Schruefer, Dirk W. Schubert, Andreas Arkudas, Annika Kengelbach-Weigand, Raymund E. Horch, Rafael Schmid
Summary: This study compared the mechanical properties and the behavior of human umbilical vein endothelial cells (HUVECs) in three hydrogels (Alg/HA/Gel, ADA-GEL, and GelMA) for vascular tissue engineering. ADA-GEL and GelMA showed promising results for cell survival, migration, and sprouting, while Alg/HA/Gel did not provide a good environment for long-term survival of HUVECs.
BIOENGINEERING-BASEL
(2023)
Article
Surgery
Ingo Ludolph, Andreas Arkudas, Wibke Muller-Seubert, Aijia Cai, Raymund E. Horch
Summary: Lymphadenectomy is an essential part of surgical tumor therapy despite potential complications, and conservative or surgical treatments are available for secondary lymphedema.
Meeting Abstract
Oncology
Chiara-Sophia Kutz, Tina Jost, Luitpold Distel, Annika Kengelbach-Weigand, Theresa Hauck, Raymund E. Horch
ONCOLOGY RESEARCH AND TREATMENT
(2022)