Article
Chemistry, Multidisciplinary
Qiao Jiang, Yingxu Shang, Yiming Xie, Baoquan Ding
Summary: DNA molecules can be repurposed to construct artificial architectures, including nanoscale structures, using techniques such as DNA origami. These precisely fabricated DNA nanomaterials have a wide range of applications and are particularly promising for drug delivery platforms. This article presents the latest advances and challenges in DNA-origami-based platforms for regulating biological functions and delivering versatile drugs.
ADVANCED MATERIALS
(2023)
Article
Polymer Science
Ahmed Shaker Ali, Mohsen Geza Alrashedi, Osama Abdelhakim Aly Ahmed, Ibrahim M. Ibrahim
Summary: This study investigated the use of a pulmonary delivery system of hydroxychloroquine (HCQ) in nanostructured lipid carriers (HCQ-NLCs) to improve its effectiveness and reduce adverse reactions in the treatment of COVID-19. The results showed that HCQ-NLCs significantly attenuated acute lung injury and improved inflammatory markers and lung tissue alterations in vivo.
Article
Cardiac & Cardiovascular Systems
Nans Florens, Rajesh K. Kasam, Valeria Rudman-Melnick, Suh-Chin Lin, Vikram Prasad, Jeffery D. Molkentin
Summary: This study investigated the mechanism of cardiac remodeling induced by acute kidney injury (AKI) using mouse models. The results showed that IL-33 released from the kidney during AKI directly affected cardiac myocytes, leading to cardiac hypertrophy and cardiomyopathy. These findings suggest that antagonizing the IL-33/ST2 axis may have cardioprotective effects in patients with kidney disease.
Article
Biochemistry & Molecular Biology
Erik De Luca, Yang Wang, Igor Baars, Federica De Castro, Marco Lolaico, Danilo Migoni, Cosimo Ducani, Michele Benedetti, Bjorn Hogberg, Francesco Paolo Fanizzi
Summary: The DNA origami method has revolutionized the field of DNA nanotechnology, offering potential as a promising vehicle for therapeutic delivery. This study explores the use of wireframe DNA origami as carriers of platinum compounds in cancer therapy, focusing on cisplatin and phenanthriplatin as model compounds. The findings demonstrate the effectiveness of the ball-like origami nanostructure loaded with phenanthriplatin in reducing cell viability in a specific cancer cell line. These results provide valuable insights into the potential of wireframe DNA origami as a highly stable carrier for long time-release purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Christopher R. Lucas, Patrick D. Halley, Amjad A. Chowdury, Bonnie K. Harrington, Larry Beaver, Rosa Lapalombella, Amy J. Johnson, Erin K. Hertlein, Mitch A. Phelps, John C. Byrd, Carlos E. Castro
Summary: The study demonstrates that DNA origami (DO) nanotechnology can be used to construct precise nanostructures capable of functionalization and has a wide range of biomedical applications. DO structures distribute well systemically, eliciting a gradually diminishing mild pro-inflammatory response, while being non-toxic.
Article
Nanoscience & Nanotechnology
Yun Zeng, Peng Chang, Jingwen Ma, Ke Li, Chunhong Zhang, Yingying Guo, Hanrui Li, Qingxia Zhu, Huifang Liu, Wenjing Wang, Yuwei Chen, Dan Chen, Xu Cao, Yonghua Zhan
Summary: In this study, we developed a DNA origami-based theranostic nanoplatform to explain the variation of tumor hypoxia during chemotherapy. Through in vitro and in vivo experiments, we demonstrated that the nanoplatform can release drugs and alleviate tumor hypoxia. Photoacoustic and fluorescence imaging were used to evaluate these effects.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Multidisciplinary
Wantao Tang, Ting Tong, Hong Wang, Xuehe Lu, Changping Yang, Yushuai Wu, Yuang Wang, Jianbing Liu, Baoquan Ding
Summary: DNA nanostructures have been widely used in drug delivery systems. This study presents a DNA origami-based CRISPR/Cas9 gene editing system for efficient gene therapy in vivo. The designed DNA origami structure can recruit and load the sgRNA/Cas9 complex for targeted gene editing, and achieve effective drug delivery.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Wu Yushuai, Shang Yingxu, Jiang Qiao, Ding Baoquan
Summary: This minireview summarizes the research progress of drug delivery systems based on the DNA origami technique and discusses the future development prospects and challenges in this field.
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
(2022)
Article
Chemistry, Multidisciplinary
Tiantian Wu, Hong Wang, Run Tian, Shuang Guo, Yuhui Liao, Jianbing Liu, Baoquan Ding
Summary: In this study, a DNA origami-based bactericide was developed for efficient treatment of infected wounds by organizing DNAzymes and broad-spectrum antibiotics, promoting healing process.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Polymer Science
Souvik Ghosal, Sagar Bag, Sudipta Bhowmik
Summary: Rapid breakthroughs in nucleic acid nanotechnology have driven the creation of nano-assemblies with programmable design, biocompatibility, and biosafety. DNA origami enables the precise organization of nanostructures and serves as the foundation for arranging functional materials in various applications. DNA origami facilitates the development of next-generation drug vectors and other biomedical strategies. This paper summarizes the synthesis of DNA origami and highlights its drug encapsulation ability and potential in biomedical sciences.
Review
Chemistry, Physical
Jorieke Weiden, Maartje M. C. Bastings
Summary: DNA nanostructures have emerged as a versatile platform for controlled drug delivery, offering unparalleled control over structural and functional parameters. Recent applications in therapeutic drug delivery show promising alternatives to conventional nanomaterials, but further research is needed to understand biodistribution, final fate, and controlled drug release aspects.
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Zhimei He, Wenjie Xiang, Qin Fan, Lianhui Wang, Jie Chao
Summary: A rectangle DNA origami nanostructure with doxorubicin-derived prodrugs targeting a tumor cell-specific enzyme (NQO1) is created. By combining the high drug payload of DNA origami and NQO1-activated chemotherapy, this nanosystem exhibits therapeutic selectivity for NQO1-overexpressing MCF-7 cells compared to healthy L02 cells, presenting a promising approach for precision cancer therapy.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yuan Liu, Jiaxin Wang, Lijun Sun, Bin Wang, Qiang Zhang, Xiaokang Zhang, Ben Cao
Summary: With the advancement of nanotechnology, DNA molecules have evolved from being solely carriers of genetic information to versatile materials with great potential in drug delivery and disease diagnosis. In this study, ladder-shaped drug carriers based on DNA nanoladders were developed. By incorporating nicks into each rung, the nanoladder structure was designed to counteract stability issues and provide drug loading sites. Experimental results showed that the designed drug-carrying function was feasible, demonstrating the potential application of this novel design approach in various fields such as bioimaging, biosensing, and targeted therapy.
Review
Chemistry, Multidisciplinary
Daniel Fu, John Reif
Summary: Structural DNA nanotechnology allows for the design and assembly of diverse DNA nanostructures with both static and dynamic properties, opening up possibilities for applications in medicine, biosensing, nanofabrication, and other interdisciplinary fields. This pioneering biotechnology has the potential to define and drive specific directions of progress in nanoscience applications.
APPLIED SCIENCES-BASEL
(2021)
Review
Engineering, Biomedical
Siyuan Zhang, Xin-Yue Lou, Linlin Liu, Ying-Wei Yang
Summary: DNA origami technology, a unique type of DNA nanotechnology, has attracted much attention from researchers and is applied in various fields. DNA origami nanostructures are endowed with excellent programmability and addressability and show outstanding biocompatibility in bio-related applications, especially in cancer treatment.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Nanoscience & Nanotechnology
Wei Li, Chengshi Wang, Zhenghao Wang, Liping Gou, Ye Zhou, Ge Peng, Min Zhu, Jiayi Zhang, Ruoqing Li, Hengfan Ni, Lei Wu, Wanli Zhang, Jiaye Liu, Yali Tian, Zhong Chen, Yuan-Ping Han, Nanwei Tong, Xianghui Fu, Xiaofeng Zheng, Per-Olof Berggren
Summary: A novel bioscaffold ILGel was developed to accelerate diabetic alveolar bone injury healing, promoting M2 macrophage polarization and osteogenesis through sustained release of IL-10 cytokine. The findings suggest the potential application of DNA hydrogel bioscaffold for cytokine-based immunotherapy in diabetic alveolar bone injury and other periodontal diseases.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Gastroenterology & Hepatology
Tianci Zhang, Ruofei Zhang, Guangfu Zhao, Wei Liu, Liwei Pan, Ying Tong, Mingshan Jiang, Hu Zhang, Zhixiong Xiao, Stephen J. Pandol, Xiansheng Fu, Yuan-Ping Han, Xiaofeng Zheng
Summary: Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, and its etiology is still poorly understood. This study found that autophagy in the intestinal tissue of IBD mice was persistently activated. Chlorophyllin (CHL), a water-soluble derivative of chlorophyll, can attenuate colitis by regulating autophagy and inflammation. Therefore, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Xiaofeng Zheng, Qing Wei Calvin Ho, Minni Chua, Olga Stelmashenko, Xin Yi Yeo, Sneha Muralidharan, Federico Torta, Elaine Guo Yan Chew, Michelle Mulan Lian, Jia Nee Foo, Sangyong Jung, Sunny Hei Wong, Nguan Soon Tan, Nanwei Tong, Guy A. Rutter, Markus R. Wenk, David L. Silver, Per-Olof Berggren, Yusuf Ali
Summary: Western-type diets, high in saturated fatty acids (SFAs), are associated with obesity and diabetes. This study reveals that SFAs, but not unsaturated fatty acids (USFAs), reduce lipid droplets (LDs) in pancreatic beta cells. SFAs induce the degradation of fat storage-inducing transmembrane protein 2 (FIT2), inhibiting LD formation. Reduction of FIT2 in beta cells impairs cellular functions and exacerbates diet-induced diabetes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Qing Wei Calvin Ho, Xiaofeng Zheng, Yusuf Ali
Summary: Ceramides, a class of sphingolipids, play a role in various skin disorders and metabolic diseases. Recent research has focused on identifying specific ceramide species and their effects on different diseases, and has found ceramides linked to metabolic dysfunction and cardiac decline.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Longhui Yuan, Jingchao Yang, Fei Liu, Lan Li, Jingping Liu, Younan Chen, Jingqiu Cheng, Yanrong Lu, Yujia Yuan
Summary: Macrophage infiltration is a common characteristic of acute kidney injury (AKI). Exosomes-mediated cell communication between tubular epithelial cells (TECs) and macrophages (M phi) have been suggested to be involved in AKI. In this study, it was found that M phi-derived exosomes from AKI mice caused mitochondrial damage and induced TECs injury. The upregulation of miR-195a-5p in M phi(AKI) was identified, and it was revealed that miR-195a-5p may be produced in infiltrated M phi and shuttled into TECs via exosomes. Inhibition of miR-195a-5p alleviated the mitochondrial dysfunction and cell injury induced by exosomes from AKI mice.
Article
Genetics & Heredity
Min Zhu, Guangneng Liao, Xiaohong Liu, Xu Luo, Gen Chen, Lingyun Wang, Chengshi Wang, Wen Liu, Xin Zeng, Yanrong Lu, Guang Yang, Jingqiu Cheng, Younan Chen
Summary: The loss of beta cell mass and function in aged population is closely related to the prevalence of Type 2 diabetes. This study focuses on the causal relations between aging and age-related pancreatic islets degeneration using rhesus monkeys as an animal model. The findings indicate that the decreased islet size, compromised insulin secretion, and loss of beta cell identity markers are key molecular changes in islet senescence.
Article
Evolutionary Biology
Chuang Zhou, Xiaofeng Zheng, Kaize Feng, Kexin Peng, Yanni Zhang, Guangqing Zhao, Yang Meng, Li Zhang, Bisong Yue, Yongjie Wu
Summary: In this study, the whole genome of the Tibetan partridge was sequenced to explore its genetic adaptation mechanisms to high-altitude environments and determine its phylogenetic relationship with typical pheasants. Additionally, genes related to hypoxia and changes in gene families associated with energy metabolism and immune system were identified. These findings provide important genomic data for understanding the evolutionary history and adaptation strategies of the Tibetan partridge.
JOURNAL OF HEREDITY
(2023)
Article
Respiratory System
Charlie Strange, N. Gerard McElvaney, Claus F. Vogelmeier, Marcos Marin-Galiano, Michaela Buch-Haensel, Xiang Zhang, Younan Chen, Oliver Vit, Marion Wencker, Kenneth R. Chapman
Summary: Acute exacerbations of COPD have an effect on PD15 lung density in alpha(1)-antitrypsin deficiency. AECOPD within 2 weeks has a significant impact on PD15, lasting less than 6 weeks. Patient characteristics have minimal correlation with PD15, and an exacerbation-free period before CT scan is recommended to reduce noise.
Correction
Genetics & Heredity
Min Zhu, Guangneng Liao, Xiaohong Liu, Xu Luo, Gen Chen, Lingyun Wang, Chengshi Wang, Wen Liu, Xin Zeng, Yanrong Lu, Guang Yang, Jingqiu Cheng, Younan Chen
Article
Chemistry, Multidisciplinary
Zilu Ge, Wei Li, Renliang Zhao, Wei Xiong, Dong Wang, Yunfeng Tang, Qian Fang, Xiangtian Deng, Zhen Zhang, Yaojia Zhou, Xiaoting Chen, Yue Li, Yanrong Lu, Chengshi Wang, Guanglin Wang
Summary: Tendon stem/progenitor cells (TSPCs) therapy using a DNA hydrogel delivery system shows promising results in enhancing tendon injury repair. Encapsulating TSPCs in a DNA hydrogel creates an artificial extracellular matrix (ECM) microenvironment that provides nutrition and protection for the cells. TSPCs-Gel injections promote tendon healing and improve the mechanical properties of injured tendons. RNA-sequencing analysis also suggests potential gene and signaling pathway targets for further treatment strategies.
Article
Orthopedics
Zilu Ge, Mingyu Yang, Danfeng Wei, Dong Wang, Renliang Zhao, Xiangtian Deng, Yunfeng Tang, Qian Fang, Zhencheng Xiong, Chengshi Wang, Guanglin Wang, Wei Li, Kanglai Tang
Summary: This study investigates the therapeutic effects of inhibiting IKK beta using a DNA supramolecular hydrogel incorporating ACHP in a rat model of Achilles tendinopathy. The results show that ACHP-Gel significantly enhances the solubility and release-retention time of ACHP, reduces inflammation, and promotes tendon healing. The findings suggest the potential of DNA supramolecular hydrogel as a delivery system for ACHP in the treatment of tendinopathy.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2023)
Article
Medicine, Research & Experimental
Qing Zheng, Min Zhu, Xin Zeng, Wen Liu, Fudong Fu, Xiaoyu Li, Guangneng Liao, Yanrong Lu, Younan Chen
Summary: Nonalcoholic fatty liver disease (NAFLD) is a common chronic liver disease, and there is no effective treatment for its advanced stage, nonalcoholic steatohepatitis (NASH). This study compared and evaluated five different NAFLD mouse models, analyzing their characteristics and suitability for research and drug development. The high-fat diet (HFD) model showed early insulin resistance and mild liver steatosis, while the high-fat, high-fructose, and high-cholesterol diet (FFC) model exacerbated glucose and lipid metabolism disorders. The FFC combined with streptozotocin (STZ) model accelerated the pathologic process of NASH, and the STAM model showed the fastest formation of fibrosis nodules.
LABORATORY INVESTIGATION
(2023)