Article
Chemistry, Multidisciplinary
Hongxia Zhao, Chao Liu, Wenlong Ding, Ling Tang, Yu Fang, Yulin Chen, Linzhen Hu, Ying Yuan, Dong Fang, Shixian Lin
Summary: This study reports an approach to greatly increase the binding energy of cation-pi interactions by replacing Trp in the aromatic box with an electron-rich Trp derivative. By using this approach, the binding affinity between histone H3K4me3 and its reader can be significantly enhanced, and a high-affinity H3K4me3 Super-Reader can be constructed.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Multidisciplinary Sciences
Aleksandra Arsic, Cathleen Hagemann, Nevena Stajkovic, Timm Schubert, Ivana Nikic-Spiegel
Summary: This study establishes click labeling in living neurons and demonstrates its application in microscopy research of neurofilament light chain (NFL). The combination of click labeling with CRISPR/Cas9 genome engineering allows for tagging endogenous NFL. Due to its versatility and compatibility with advanced microscopy techniques, click labeling is expected to contribute to novel discoveries in the field of neurobiology.
NATURE COMMUNICATIONS
(2022)
Review
Biotechnology & Applied Microbiology
Jian Zhang, Qingxiao Pang, Qi Wang, Qingsheng Qi, Qian Wang
Summary: Genetically encoded biosensors have a wide range of detectable signals and potential applications, with continuous improvement in performance and functions. Strategies for tuning biosensors and expanding input signals have been summarized. Analysis of different output signals and their practical requirements in biotechnology, biomedical applications, and environmental safety provides insight for improving design and optimization of biosensors in various application scenarios.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Andrew L. Markhard, Jason G. McCoy, Tsz-Leung To, Vamsi K. Mootha
Summary: Oxygen is crucial for supporting life, but methods for manipulating its supply with high spatiotemporal resolution in cells and organisms are lacking. Researchers have introduced a genetic system called SNORCL, which allows for on-demand generation of molecular oxygen inside living cells. This technology has immediate research applications and has the potential for widespread use in research, biotechnology, and medicine.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Yasmin Aydin, Irene Coin
Summary: Noncanonical amino acids (ncAAs) are powerful tools for studying protein interactions. This article provides an overview of ncAAs for photo- and chemical crosslinking, focusing on recent developments and their applications in capturing protein-protein interactions, investigating protein function, stabilizing protein complexes for structural studies, and developing covalent drugs. The technology has matured beyond proof-of-principle demonstrations and is now being used in modern integrative approaches.
Article
Chemistry, Analytical
Kun Zhang, Ying Lan, Fanfan Wang, Zhiming Gou, Mei Yan, Yujing Zuo
Summary: In this study, a switchable targeting polysiloxane platform was constructed, and fluorescent probes targeting lysosomes and mitochondria were successfully prepared. Real-time detection of peroxynitrite (ONOO-) in lysosomes and mitochondria during ferroptosis was achieved. The occurrence of autophagy during late ferroptosis and the interaction between mitochondria and lysosomes were observed. This switchable targeting polysiloxane functional platform is expected to broaden the application of polymeric materials in bioimaging and provide a powerful tool for further understanding of the ferroptosis process.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Multidisciplinary
Toon Van Thillo, Vincent Van Deuren, Peter Dedecker
Summary: Genetically-encoded biosensors enable the visualization of dynamic biochemical events within living systems, providing new insights. Over the past 25 years, biosensor development has improved their performance and expanded the repertoire. This feature article presents recent advances and future directions in biosensor development.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xingxiu Pan, Mina L. Heacock, Evana N. Abdulaziz, Sara Violante, Austin L. Zuckerman, Nirajan Shrestha, Canglin Yao, Russell P. Goodman, Justin R. Cross, Valentin Cracan
Summary: Impaired redox metabolism plays a critical role in the development of various diseases. This study introduces a novel genetically encoded tool to promote reductive stress in living cells. By using this tool, metabolic and transcriptomic signatures of NADH reductive stress are found to be cellular background specific.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Cell Biology
Carolina dos Santos Passos, Yun-Seok Choi, Christopher D. Snow, Tingting Yao, Robert E. Cohen
Summary: The study developed sensors for specifically ubiquitinated nucleosomes to monitor signaling from endogenous DNA damage, distinguishing the roles of 53BP1 and BARD1 in this histone posttranslational modification.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Elena L. Sokolinskaya, Lidia Putlyaeva, Vasilisa S. Polinovskaya, Konstantin A. Lukyanov
Summary: The researchers designed genetically encoded fluorescent sensors for real-time detection of PLpro activity in live cells of SARS-CoV-2. These sensors can be used to detect early stages of virus propagation and screen enzyme inhibitors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Pengkai Sun, Zhenxing Zhang, Bin Wang, Caiyun Liu, Chao Chen, Ping Liu, Xinjian Li
Summary: The authors developed a fluorescent biosensor named BioITA for detecting the dynamics of itaconate in subcellular compartments of living macrophages. They found that STING activation induces itaconate production and successfully reported the elevation of itaconate level in activated macrophages derived from mice.
NATURE COMMUNICATIONS
(2022)
Article
Biophysics
Guoliang Zheng, Liang Zhao, Deyu Yuan, Jia Li, Gang Yang, Danxia Song, Hui Miao, Linjuan Shu, Xianming Mo, Xiaoding Xu, Ling Li, Xu Song, Yongyun Zhao
Summary: A novel label-free fluorescent turn-on biosensor for monitoring ATP in living bacterias and mammalian cells was developed, utilizing heterobifunctional aptamers with high sensitivity. A tandem method combining Broc-ATPs with 3 x F30 three-way junction scaffold was established to construct an intracellular biosensor for sensitive and specific imaging of ATP dynamics in mammalian cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Ze Fan, Cai-Xia Dou, Li-Juan Tang, Fenglin Wang, Jian-Hui Jiang
Summary: Genetically encoded sensors with a modular design have been developed for ratiometric and multiplexed imaging of small molecules in live cells. The sensors use aptazyme as a recognition module and light-up RNA aptamer as a signal reporter. The design allows for quantitative imaging and detection of small molecules, and the versatility of the approach is demonstrated by replacing the aptazymes for imaging different molecules.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Rahila Nazir, Mohd Mohsin, Tariq Omar Siddiqi
Summary: A genetically encoded FRET-based sensor has been developed for studying the efflux of gold in living cells. This highly selective and sensitive probe can withstand changes in variable pH ranges and is capable of non-invasive real-time measurement of gold in living cells.
Article
Multidisciplinary Sciences
Yasmin Aydin, Thore Bottke, Jordy Homing Lam, Stefan Ernicke, Anna Fortmann, Maik Tretbar, Barbara Zarzycka, Vsevolod V. Gurevich, Vsevolod Katritch, Irene Coin
Summary: Understanding the molecular basis of arrestin-mediated regulation of GPCRs is crucial for deciphering signaling mechanisms. However, structural studies are challenging due to the dynamic nature of GPCR-arrestin complexes. In this study, the interaction between arrestin-2 and PTH1R was dissected using crosslinking amino acids in live cells. The integrative approach revealed the structure, dynamics, and flexible regions of the PTH1R-arrestin2 complex.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Bing-bing Hao, Xiao-jing Li, Xing-long Jia, Yu-xing Wang, Lin-hui Zhai, Duan-zhuo Li, Jie Liu, Die Zhang, Yu-lu Chen, Yong-hu Xu, Sang-kyu Lee, Guo-feng Xu, Xiao-hua Chen, Yong-jun Dang, Bin Liu, Min-jia Tan
ACTA PHARMACOLOGICA SINICA
(2020)
Article
Biochemistry & Molecular Biology
Huijie Guo, Mei Pu, Yusi Tai, Yuxiang Chen, Henglei Lu, Junwen Qiao, Guanghui Wang, Jing Chen, Xinming Qi, Ruimin Huang, Zhouteng Tao, Jin Ren
Summary: The study revealed that miR-30b-5p can inhibit TFEB-dependent gene transcription by binding to CLEAR elements in the nucleus, thus affecting lysosomal biogenesis and autophagy. Knocking out miR-30b-5p increases TFEB transactivation, leading to increased expression of autophagy and lysosomal biogenesis-related genes.
CELL DEATH AND DIFFERENTIATION
(2021)
Article
Oncology
Lin Yang, Jingya Sun, Meiqian Li, Yiming Long, Dianzheng Zhang, Hongqian Guo, Ruimin Huang, Jun Yan
Summary: The study highlights the association between hypercholesterolemia-induced oxidized ldL and urinary bladder cancer, with CD36/JAK2/STAT3 signaling axis being potential therapeutic targets. ox-LDL could serve as a biomarker for aggressive UBC.
Article
Chemistry, Medicinal
Dan Wei, Hanlin Wang, Qinghe Zeng, Wenjing Wang, Bingbing Hao, Xule Feng, Peipei Wang, Ning Song, Weijuan Kan, Guifang Huang, Xiaoyu Zhou, Minjia Tan, Yubo Zhou, Ruimin Huang, Jia Li, Xiao-Hua Chen
Summary: TNBC is highly aggressive and clinically challenging due to limited treatment options. Targeting transcription-associated CDK9 with a novel degrader compound shows great promise in inhibiting TNBC cell growth and inducing apoptosis by downregulating transcriptional targets like MYC. This study establishes compound 45 as a potent and efficacious CDK9 degrader for potential targeted therapy in TNBC.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Cell & Tissue Engineering
Chen Ma, Ludi Zhang, Ting He, Huiying Cao, Xiongzhao Ren, Chenhui Ma, Jiale Yang, Ruimin Huang, Guoyu Pan
Summary: Raman spectroscopy was successfully employed to identify different stages of ProliHHs during dedifferentiation process, providing a new approach to simultaneously trace multiple changes of cellular components from somatic cells to progenitor cells.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Xinnan Zhang, Luting Wang, Ruimin Huang, Jingbo Wang, Qifan Yan
Summary: Capmatinib is an FDA-approved drug for metastatic non-small cell lung cancer with MET-exon 14 skipping. By introducing a perfluoro-tert-butyl group with nine chemically identical fluorine atoms on Capmatinib, a targeted F-19 magnetic resonance imaging (MRI) probe called perfluoro-tert-butyl group-derived Capmatinib (9F-CAP) was developed. The study demonstrated that 9F-CAP specifically targeted cMET protein through molecular docking simulation, surface plasmon resonance (K-d of 40.7 μM), IC50 (168 nM) assays, Annexin V, and cytotoxicity assays. Therefore, the targeted imaging capability of 9F-CAP is of great significance for the preoperative diagnosis of specific cancers.
Article
Chemistry, Multidisciplinary
An-Di Guo, Ke-Nian Yan, Hao Hu, Linhui Zhai, Teng-Fei Hu, Haixia Su, Yijia Chi, Jinyin Zha, Yechun Xu, Dongxin Zhao, Xiaojie Lu, Yong-Jiang Xu, Jian Zhang, Minjia Tan, Xiao-Hua Chen
Summary: The study developed a light-induced lysine (K) enabled crosslinking (LIKE-XL) strategy for spatiotemporal and global profiling of DNA-protein interactions. By capturing weak and transient DNA-protein interactions, LIKE-XL can discover low-affinity transcription-factor/DNA interactions using specific DNA baits and identify previously unknown binding sites. Additionally, it can decipher the dynamics of the transcription factor subproteome in response to drug treatment and identify downstream target transcription factors, providing insight into their dynamic transcriptional networks. LIKE-XL offers a complementary method to expand the DNA-protein profiling toolbox and map accurate DNA-protein interactomes for better understanding of protein function in health and disease.
Article
Multidisciplinary Sciences
Yunfu Lu, Feifei Chen, Qingmin Zhao, Qiao Cao, Rongrong Chen, Huiwen Pan, Yanhui Wang, Haixin Huang, Ruimin Huang, Qian Liu, Min Li, Taeok Bae, Haihua Liang, Lefu Lan
Summary: Inhibition of TarO, the first enzyme in the wall teichoic acid biosynthetic pathway, decreases the expression of key virulence determinants in community-associated MRSA, and this effect is linked to the activation of the VraRS two-component system. The findings suggest that targeting TarO could be a potential strategy for anti-virulence therapy in MRSA infections.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Organic
Yandan Bao, Minyan Xing, Naylor Matthew, Xiaohua Chen, Xuan Wang, Xiaojie Lu
Summary: This paper presents a set of on-DNA methodologies for constructing DNA-linked libraries with high cyclization and DNA integrity. These methodologies include OPA-mediated three-component cyclization and photoredox chemistries, which can generate novel isoindole, isoindoline, indazolone, and bicyclic scaffolds in good to excellent conversions.
Article
Chemistry, Multidisciplinary
Yan Xu, Dingqiang Fu, Yi Yuan, Yan Peng, Juan Dong, Feng Du, Xin Huang, Guangxun Li, Xiaohua Chen, Qiwei Wang, Zhuo Tang
Summary: The ability to regulate protein abundance provides opportunities for disease treatments. PROTAC, as a promising technology, utilizes RNA aptamers to target and degrade multiple target proteins. Aptamer-based PROTAC technology offers a general strategy for targeted protein degradation in drug discovery and biological research.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
An-Di Guo, Ke-Huan Wu, Xiao-Hua Chen
Summary: Chemical modification of proteins using a light-induced lysine-selective approach has been shown to have reliable chemoselectivity, excellent efficiency, temporal control, and biocompatibility under simple and mild conditions, both in vitro and in living systems. This protocol demonstrates the generality and accessibility for direct and rapid functionalization of diverse native proteins, suggesting a new avenue for bioconjugation, medicinal chemistry, and chemical biology.
Article
Multidisciplinary Sciences
An-Di Guo, Dan Wei, Hui-Jun Nie, Hao Hu, Chengyuan Peng, Shao-Tong Li, Ke-Nian Yan, Bin-Shan Zhou, Lei Feng, Chao Fang, Minjia Tan, Ruimin Huang, Xiao-Hua Chen
NATURE COMMUNICATIONS
(2020)
Article
Integrative & Complementary Medicine
Yu Dong, Jiakuan Liu, Zesheng Xue, Jingya Sun, Zhengnan Huang, Yifeng Jing, Bangmin Han, Bing Shen, Jun Yan, Ruimin Huang
BMC COMPLEMENTARY MEDICINE AND THERAPIES
(2020)