Journal
BRITISH JOURNAL OF NUTRITION
Volume 114, Issue 8, Pages 1123-1131Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114515002962
Keywords
Black elderberry extract; Obesity; Insulin resistance; Diet-induced obese mice; Inflammation; Molecular nutrition
Categories
Funding
- USDA AFRI [CONS2014-06619]
- USDA Hatch [CONS00934]
Ask authors/readers for more resources
Dietary anthocyanins have been shown to reduce inflammation in animal models and may ameliorate obesity-related complications. Black elderberry is one of the richest sources of anthocyanins. We investigated the metabolic effects of anthocyanin-rich black elderberry extract (BEE) in a diet-induced obese C57BL/6J mouse model. Mice were fed either a low-fat diet (n 8), high-fat lard-based diet (HFD; n 16), HFD +0.25 % (w/w) BEE (0.25 %-BEE; n 16) or HFD +1.25 % BEE (1.25 %-BEE; n 16) for 16 weeks. The 0.25 % BEE (0.034 % anthocyanin, w/w) and 1.25 % BEE (0.17 % anthocyanin, w/w) diets corresponded to estimated anthocyanin doses of 20-40 mg and 100-200 mg per kg of body weight, respectively. After 16 weeks, both BEE groups had significantly lower liver weights, serum TAG, homoeostasis model assessment and serum monocyte chemoattractant protein-1 compared with HFD. The 0.25 %-BEE also had lower serum insulin and TNF alpha compared with HFD. Hepatic fatty acid synthase mRNA was lower in both BEE groups, whereas PPAR gamma 2 mRNA and liver cholesterol were lower in 1.25 %-BEE, suggesting decreased hepatic lipid synthesis. Higher adipose PPAR. mRNA, transforming growth factor beta mRNA and adipose tissue histology suggested a pro-fibrogenic phenotype that was less inflammatory in 1.25 %-BEE. Skeletal muscle mRNA expression of the myokine IL-6 was higher in 0.25 %-BEE relative to HFD. These results suggest that BEE may have improved some metabolic disturbances present in this mouse model of obesity by lowering serum TAG, inflammatory markers and insulin resistance.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available