Article
Agriculture, Multidisciplinary
Tamer Ali Sweellum, Deyala M. Naguib
Summary: This study evaluates the role of tomato potato onion intercropping in inducing tomato resistance against Fusarium oxysporum. The intercropping was found to enhance tomato resistance by improving soil enzymes and antifungal enzyme activity, releasing compounds that initiate defense response in tomato plants. This defense response is characterized by elevated antioxidant capacity, antagonistic enzymes against oxidative stress, and improved secondary metabolism.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Agronomy
Nawal E. Al-Hazmi, Deyala M. Naguib
Summary: This study aims to find a simple agricultural waste processing method to improve soil properties and enhance plant growth and defense against pathogen infection by extracting polysaccharides and applying them to the soil.
Article
Agronomy
Nicolas Oliveira de Araujo, Mirelle Nayana de Sousa Santos, Fernanda Ferreira de Araujo, Mario Leno Martins Veras, Jean Paulo de Jesus Tello, Rafaela da Silva Arruda, Karen Klotz Fugate, Fernando Luiz Finger
Summary: The sensitivity of different sweet potato cultivars to chilling injury is associated with the activity of their antioxidant systems, with cold-sensitive cultivars showing membrane integrity loss and lipid peroxidation, while cold-tolerant cultivars are able to detoxify hydrogen peroxide through a balanced antioxidant system to alleviate chilling injury symptoms.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Renu Bhardwaj, Sunil Pareek, J. Abraham Dominguez-Avila, Gustavo A. Gonzalez-Aguilar, Daniel Valero, Maria Serrano
Summary: Exogenous melatonin treatment improves cold resistance of mangoes, with 'Langra' mangoes showing the greatest benefit and 'Gulab Jamun' mangoes showing the least. The positive effect is associated with an increase in endogenous melatonin concentration, which prevents reactive oxygen accumulation and stimulates non-enzymatic antioxidants. 'Langra' mangoes also exhibit enhanced antioxidant activity and cold resistance enzyme performance.
Article
Plant Sciences
Mostafa Abdollahi Bakhtiari, Pooran Golkar
Summary: The study showed that using chitosan (CHT) as an elicitor in callus cultures of Lepidium sativum can significantly enhance total phenolics content, PAL activity, antioxidant capacity, and callus fresh weight. Additionally, the treatment with CHT also led to a substantial increase in lepidine accumulation.
JOURNAL OF PLANT GROWTH REGULATION
(2022)
Article
Multidisciplinary Sciences
Yunhao Sun, Dianxian Cai, Di Qin, Jialiang Chen, Yutong Su, Xiaoying Zheng, Zhen Meng, Jie Zhang, Lina Xiong, Zhangyong Dong, Ping Cheng, Xiaoming Peng, Guohui Yu
Summary: GZM, a plant protection preparation containing lauryl alcohol, enhances the performance of peanut plants in both laboratory and field conditions by increasing the production of specific lysophospholipids, phenylpropanoids, flavonoids, and wax. It also improves crop immunity, yield, and quality, and inhibits the growth of some pathogenic fungi.
Article
Biochemistry & Molecular Biology
Yu Qin, Qiue Li, Qiuju An, Dexin Li, Sipei Huang, Yongyang Zhao, Weijia Chen, Jiayu Zhou, Hai Liao
Summary: Drought is a key threat to plants and agriculture, and the role of the enzyme phenylalanine ammonia lyase (PAL) in drought tolerance has been explored in this study. A PAL member called FuPAL1 was isolated from the alpine pharmaceutical plant Fritillaria unibracteata. FuPAL1 was found to play a role in the ammonization process and was involved in substrate binding and catalysis. Transgenic Arabidopsis plants with FuPAL1 showed increased levels of lignin and salicylic acid and enhanced drought tolerance. These findings suggest that FuPAL1 could be a valuable target for improving drought resistance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Reda E. Abdelhameed, Rabab A. Metwally, Shereen A. Soliman
Summary: The study found that Bacillus amyloliquefaciens RaSh1 can enhance the growth of pepper plants and suppress the pathogenic effect of Alternaria alternata by modulating antioxidant defense and phytohormone levels.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agricultural Engineering
Ai-Lian Liu, Yu-Hao Wang, Tian-Yu Wang, Yue Zhu, Peng Wu, Liang-Jun Li
Summary: Secondary active substances have been widely used in various industrial fields due to their unique biological activities. Euryale ferox, also known as prickly waterlily, is an important commercial crop with numerous health functions, but information on its secondary metabolites is limited. In this study, 589 secondary active substances were identified in different tissues of E. ferox using UHPLC-MS/MS technique, with polyphenols being the predominant compound. Flavonoids were found to vary the most among the five tissues. The antioxidant evaluation revealed that the seed shell possessed excellent antioxidant properties. The study also demonstrated the remarkable activity of EfPAL4 in the biosynthesis of polyphenols and flavonoids through enzyme activity assays and overexpression in Arabidopsis. These findings provide important insights for the future industrial application of E. ferox.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Feiyan Xue, Zihui Liu, Yue Yu, Yangjie Wu, Yuxin Jin, Mingfeng Yang, Lanqing Ma
Summary: Researchers cloned the cDNA of Rhodotorula glutinis PAL (RgPAL) and introduced codon optimization to enhance its protein expression and enzyme activity in Escherichia coli. This improvement allowed for the successful expression and purification of the recombinant enzyme PAL-opt, which exhibited high specific activity for substrate L-phenylalanine and L-tyrosine. This bifunctional enzyme provides a promising foundation for future applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Hassan S. Al-Zahrani, Hesham E. Alharby, Khalid Rehman Hakeem, Reiaz Ul Rehman
Summary: The study found that the application of zinc can alleviate the negative effects of salt stress on plant growth to a certain extent by improving physiological and biochemical changes, including increased shoot and root length, enhanced chlorophyll and carotenoid contents, and increased enzymatic antioxidant activities.
Article
Agronomy
Emanuele La Bella, Andrea Baglieri, Ermes Ivan Rovetto, Piergiorgio Stevanato, Ivana Puglisi
Summary: The study investigated the application of Chlorella vulgaris extract on lettuce seedlings, which positively influenced the growth of seedlings, increased chlorophylls, carotenoids, protein content, and ashes in shoots. Additionally, the extract also had a positive impact on the nitrogen metabolism in shoots and increased dry matter, proteins, and ash content in roots.
Article
Biology
Niharika, Narsingh Bahadur Singh, Shubhra Khare, Ajey Singh, Vijaya Yadav, Ravi Kumar Yadav
Summary: Cytokinins play a crucial role in influencing plant metabolic and adaptive mechanisms under adversity. The study showed that kinetin (KN) can ameliorate the damaging effects of allelochemical 2-benzoxazolinone (BOA) on Vigna radiata seedlings. KN supplementation helped in growth restoration by reducing oxidative stress and improving membrane stability, enhancing antioxidant enzyme activity and total phenolic content in the seedlings.
Article
Plant Sciences
Arashdeep Singh, Hanuman Bobade, Savita Sharma, Baljit Singh, Antima Gupta
Summary: By extending the germination time and increasing the temperature, the digestibility and bioactive potential of wheat flour can be enhanced. This leads to an increase in total phenolic and flavonoid content, while also resulting in a decrease in flour viscosity and lightness values.
PLANT FOODS FOR HUMAN NUTRITION
(2021)
Article
Chemistry, Physical
Chun-Yen Hsieh, Yi-Hao Huang, Hui-Hsuan Yeh, Pei-Yu Hong, Che-Jen Hsiao, Lu-Sheng Hsieh
Summary: Bambusa oldhamii BoPAL4 exhibits broad substrate specificity, with optimal reaction pH and temperature varying for different substrates. A major substrate specificity site mutant, BoPAL4-H123F, shows improved affinity for L-phenylalanine. BoPAL4 is confirmed to utilize L-phenylalanine, L-tyrosine, and L-DOPA as substrates.
Article
Oncology
Daklallah Ahmed Almalki, Deyala M. Naguib
Summary: The study found that germinated fenugreek seed extract can effectively increase the survival rate of pancreatic cancer mice and protect pancreatic tissue, making it a potential treatment option for pancreatic cancer.
JOURNAL OF GASTROINTESTINAL CANCER
(2022)
Article
Oncology
Abdulrahman Ali Alzandi, Essam Ahmed Taher, Mohamed Azizi, Nageeb A. Al-Sagheer, Abdul Wali Al-Khulaidi, Deyala M. Naguib
Summary: This study evaluates the antibacterial activity of six wild medicinal plants from the Al Bahah region in Saudi Arabia and finds that they have a high inhibitory effect on carcinogenic bacteria associated with gastrointestinal cancers. These plant extracts could be a promising option for the development of new antibacterial products against these carcinogenic bacteria.
JOURNAL OF GASTROINTESTINAL CANCER
(2023)
Article
Chemistry, Inorganic & Nuclear
Ayat-Allah Mamdouh, Ahmed B. M. Ibrahim, Nour El-Houda A. Reyad, Tarek R. Elsayed, Isabel Cordeiro Santos, Antonio Paulo, Refaat M. Mahfouz
Summary: This paper presents the synthesis and characterization of two cluster compounds and investigates their antibacterial activity and thermal decomposition mechanisms. The nickel compound exhibits higher inhibitory activity against gram-positive bacteria and phytopathogenic bacteria, while the cobalt compound shows higher antibacterial activity against Pseudomonas aeruginosa.
JOURNAL OF CLUSTER SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Israa M. Shamkh, Mohammed Al-Majidi, Ahmed Hassen Shntaif, Peter Tan Deng Kai, Ngoc Nh-Pham, Ishrat Rahman, Dalia Hamza, Mohammad Shahbaz Khan, Maii S. Elsharayidi, Eman T. Salah, Abdullah Haikal, Modupe Akintomiwa Omoniyi, Mahmoud A. Abdalrahman, Tomasz M. Karpinski
Summary: Novel strategies using protease inhibitors (PIs) have shown great potential in controlling insects, and molecular docking and dynamics simulations have been used to investigate their effectiveness. This study focused on in silico analysis of potential inhibitory compounds against various insect proteins, demonstrating their ability to form stable complexes and exhibit inhibitory activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Mahmoud Elkazzaz, Amr Ahmed, Yousry Esam-Eldin Abo-Amer, Tamer Hydara, Abdullah Haikal, Dina N. Abd El Razek, Wafa Ali Eltayb, Xiling Wang, Tomasz M. Karpinski, Dalia Hamza, Basit Jabbar, Israa M. Shamkh
Summary: Despite ongoing research on the pathogenesis of COVID-19, much remains unknown. However, a recent study using molecular docking and molecular dynamics simulations found a high affinity between the SARS-CoV-2 spike protein and GnRHR and GPCRs. This discovery sheds light on the complex mechanisms involving neuroendocrine and peripheral organ involvement in COVID-19, and may lead to the identification of new drug targets.
Article
Agriculture, Multidisciplinary
Tamer Ali Sweellum, Deyala M. Naguib
Summary: This study evaluates the role of tomato potato onion intercropping in inducing tomato resistance against Fusarium oxysporum. The intercropping was found to enhance tomato resistance by improving soil enzymes and antifungal enzyme activity, releasing compounds that initiate defense response in tomato plants. This defense response is characterized by elevated antioxidant capacity, antagonistic enzymes against oxidative stress, and improved secondary metabolism.
JOURNAL OF PLANT DISEASES AND PROTECTION
(2023)
Article
Agronomy
Ahmed Mohamed Nabil Abdelraouf, Akram Abdelmonem Hussain, Deyala M. Naguib
Summary: The synergistic effect of using nanochitosan to deliver Pseudomonas fluorescens on its stability in the soil and induction resistance against fusarium wilt in tomato plants was investigated. The results showed that the nano-Pf treatment resulted in the highest growth and disease reduction percentage. Additionally, the treatment enhanced the defense enzyme activity in tomato plants and effectively mitigated Fusarium wilt infection.
Article
Immunology
Nawal E. Al-Hazmi, Deyala M. Naguib
Summary: Agricultural waste polysaccharides show effective antibacterial activity and antibiofilm activity against antibiotic-resistant carcinogenic bacteria. This study investigates the antibacterial mechanism of the polysaccharides.
MICROBIAL PATHOGENESIS
(2023)
Article
Agronomy
Nawal E. Al-Hazmi, Deyala M. Naguib
Summary: This study aims to find a simple agricultural waste processing method to improve soil properties and enhance plant growth and defense against pathogen infection by extracting polysaccharides and applying them to the soil.
Article
Biochemistry & Molecular Biology
Abdelmoujoud Faris, Ibrahim M. Ibrahim, Radwan Alnajjar, Hanine Hadni, Mashooq Ahmad Bhat, Muhammad Yaseen, Souvik Chakraborty, Nada Alsakhen, Israa M. Shamkh, Fazal Mabood, Ahmed M. Naglah, Ihsan Ullah, Noha Ziedan, Menana Elhallaoui
Summary: This study presents an integrated methodology that utilizes various computational techniques to design and predict new inhibitors targeting the JAK3/STAT pathway. The approach includes QSAR analysis, pharmacophore modeling, ADMET prediction, covalent docking, molecular dynamics simulations, and binding free energy calculations. A robust QSAR model was developed through the use of MLR and ANN methods, which exhibited good performance and high accuracy. The pharmacophore model effectively identified potential JAK3 inhibitors. The newly designed inhibitors showed high inhibitory potency and promising ADMET properties. The stability of the inhibitors within the JAK3 binding site was confirmed through MD simulations, and their binding affinity was supported by MM/GBSA calculations.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biotechnology & Applied Microbiology
Nawal E. Al-Hazmi, Deyala M. Naguib
Summary: Antibiotic-resistant pathogenic bacteria and oxidative stress related to their infections pose dangerous health problems. Finding safe and effective antibacterial and antioxidant agents is a global priority. Probiotics are potential candidates as antibacterial and antioxidant agents. Encapsulating probiotics on nano-chitosan enhances their antibacterial and antioxidant activities, showing promise in controlling pathogenic bacteria.
INDIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Oncology
Nawal E. Al-Hazmi, Deyala M. Naguib
Summary: This study introduces a cheap nano-peroxidase as an efficient and safe antibacterial and anti-inflammatory agent against bacteria and inflammation related to colorectal cancer. The prepared nano-peroxidase exhibited high antibacterial activity against key bacteria in CRC at very low concentrations of H2O2, and showed significantly higher anti-inflammatory activity compared to the standard drug Aspirin.
JOURNAL OF GASTROINTESTINAL CANCER
(2022)
Correction
Oncology
Abdulrahman Ali Alzandi, Deyala M. Naguib, Al-Shimaa M. Abas
JOURNAL OF GASTROINTESTINAL CANCER
(2022)
Article
Oncology
Abdulrahmaan Ali Alzandi, Deyala M. Naguib, Al-Shimaa M. Abas
Summary: Encapsulation of onion extract on nano chitosan effectively enhances its anticancer activity, reducing IC50 in different cell lines and inducing apoptosis by decreasing BCL-2 level and increasing caspase-3 and caspase-9 activity. This approach may serve as protective agents against cancer or act synergistically with cancer chemotherapy, promoting the use of natural products in cancer therapy.
JOURNAL OF GASTROINTESTINAL CANCER
(2022)