Review
Medicine, Research & Experimental
Nazmul Haque, Darius Widera, Vijayendran Govindasamy, Pratiwi Soesilawati, Noor Hayaty Abu Kasim
Summary: Cell-based regenerative therapies have the potential to treat non-communicable and degenerative diseases. Extracellular vesicles (EVs), which contain miRNAs, mRNAs, and proteins, play a role in intercellular communication. However, the regenerative potential of these molecules is still debated.
CURRENT MOLECULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Naina Soni, Suchi Gupta, Surender Rawat, Vishnu Krishnakumar, Sujata Mohanty, Arup Banerjee
Summary: This study investigates the effects of exosomes derived from different sources on immune cells. The expression levels of miRNAs varied within different MSCs and exosomes, and most miRNAs had expression profiles different from their respective sources. Exosomes from different sources had different abilities to induce neurogenesis and angiogenesis, as well as varying effects on PBMC proliferation, neutrophil survival, and NET formation.
Article
Multidisciplinary Sciences
Ricardo Malvicini, Diego Santa-Cruz, Anna Maria Tolomeo, Maurizio Muraca, Gustavo Yannarelli, Natalia Pacienza
Summary: In this study, we developed a chromatographic, scalable, and clinically compatible method to isolate and concentrate extracellular vesicles (EVs) from conditioned media, while preserving their biological activity. The method is simple, fast, and suitable for clinical production.
Article
Medicine, Research & Experimental
Allaura S. Cone, Xuegang Yuan, Li Sun, Leanne C. Duke, Michael P. Vreones, Allison N. Carrier, Stephanie M. Kenyon, Spencer R. Carver, Sarah D. Benthem, Alina C. Stimmell, Shawn C. Moseley, David Hike, Samuel C. Grant, Aaron A. Wilber, James M. Olcese, David G. Meckes
Summary: Research has shown that extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) have potential therapeutic effects for Alzheimer's disease, slowing down disease progression and improving cognitive function when administered intranasally in mice.
Review
Medicine, Research & Experimental
Huan Zhang, Xueqi Wan, Jinfan Tian, Ziyu An, Libo Liu, Xin Zhao, Yuquan Zhou, Lijun Zhang, Changjiang Ge, Xiantao Song
Summary: This review presents the cardioprotective effects of mesenchymal stem cell-derived exosomes and explores the underlying molecular mechanisms. It also discusses several efficient approaches for delivering exosomes to the heart in clinical applications and comments on the advantages and disadvantages of each method.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Pharmacology & Pharmacy
Tarek Khamis, Amira Ebrahim Alsemeh, Asma Alanazi, Asmaa Monir Eltaweel, Heba M. Abdel-Ghany, Doaa M. Hendawy, Adel Abdelkhalek, Mahmoud A. Said, Heba H. Awad, Basma Hamed Ibrahim, Dina Mohamed Mekawy, Corina Pascu, Crista Florin, Ahmed Hamed Arisha
Summary: This study investigated the therapeutic potential of breast milk mesenchymal stem cells (Br-MSCs) and their derived exosomes in chronic kidney disease (CKD). The results showed that Br-MSCs and their exosomes reduce adenine-induced renal damage by secreting antifibrotic microRNAs and enhance renal autophagy by modulating SNHG-7 expression.
Review
Engineering, Biomedical
Lorella Giovannelli, Elia Bari, Claudio Jommi, Fulvio Tartara, Daniele Armocida, Diego Garbossa, Fabio Cofano, Maria Luisa Torre, Lorena Segale
Summary: Neurodegenerative diseases are a growing healthcare burden globally. Mesenchymal stem cells (MSCs) have potential as a therapy due to their neuroregenerative, neuroprotective, and immunomodulatory properties through their secretome. This paper provides an overview of recent research on the safety and efficacy of MSC-derived secretome and extracellular vesicles (EVs) in various neurodegenerative diseases, and discusses the challenges and potential future directions for translating them into clinical use.
BIOACTIVE MATERIALS
(2023)
Review
Nutrition & Dietetics
Herminia Mendivil-Alvarado, Leopoldo Alberto Sosa-Leon, Elizabeth Carvajal-Millan, Humberto Astiazaran-Garcia
Summary: Extracellular vesicles (EVs) are active components in cellular communication that are sensitive to changes in the cellular microenvironment and are associated with disease conditions. Changes in EVs have been observed in obese patients and may contribute to the development of metabolic and cardiovascular complications, although the mechanisms are not fully understood. EVs also play a role in maintaining homeostasis and muscle repair under conditions of undernutrition.
Review
Biotechnology & Applied Microbiology
Yu Luo, Zhihua Li, Xinxin Wang, Juan Wang, Xingxiang Duan, Ruohan Li, Youjian Peng, Qingsong Ye, Yan He
Summary: Recently, there has been a growing interest in studying the use of extracellular vesicles (EVs) secreted by stem cells to repair damaged tissues or lost cells. EVs play an important role in cell-to-cell communication and are involved in various physiological and pathological processes. Among the different types of EVs, exosomes are the most important subset and carry a mass of signal molecules. The research on exosomes has generated substantial interest in the scientific community.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Giulia Brunello, Federica Zanotti, Martina Trentini, Ilaria Zanolla, Elham Pishavar, Vittorio Favero, Riccardo Favero, Lorenzo Favero, Eriberto Bressan, Massimo Bonora, Stefano Sivolella, Barbara Zavan
Summary: This study found that EXOs derived from dental pulp stem cells of different age donors have varying effects on MSCs. The younger donor group showed a more significant influence on proliferation and commitment ability.
Review
Biochemistry & Molecular Biology
Yasunari Matsuzaka, Ryu Yashiro
Summary: This review summarizes the advances in the purification, modification, and application of extracellular vesicles as a novel strategy for treating certain diseases.
Article
Immunology
Huldani Huldani, Saade Abdalkareem Jasim, Dmitry Olegovich Bokov, Walid Kamal Abdelbasset, Mohammed Nader Shalaby, Lakshmi Thangavelu, Ria Margiana, Maytham T. Qasim
Summary: Mesenchymal stem cells (MSCs) have great potential as therapeutic candidates, but their clinical application is limited due to their tumorigenic properties. Extracellular vesicles (EVs), as paracrine factors of MSCs, have gained attention as potential cell-free replacement therapy due to their low immunogenicity and tumorigenicity, as well as easy management.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Clinical Neurology
Shu-fen Liu, Lin-yi Li, Jian-long Zhuang, Mi-mi Li, Li-chao Ye, Xiang-rong Chen, Shu Lin, Chun-nuan Chen
Summary: Parkinson's disease (PD) is the second largest neurodegenerative disease and stem cell therapy, as well as exosome treatment, show potential as treatment options. However, the exact mechanisms are still unclear. This review discusses the mechanisms of exosomes in PD and explores future research directions.
FRONTIERS IN NEUROLOGY
(2022)
Review
Clinical Neurology
Maryam Abrishamdar, Maryam Sadat Jalali, Neda Yazdanfar
Summary: Parkinson's disease (PD) is a chronic neurodegenerative disease characterized by dopaminergic cell loss in the substantia nigra. Cell transplantation using mesenchymal stem cells (MSCs) is a potential therapeutic strategy for PD. MSC-derived exosomes have shown to have protective and neurodifferentiation effects, making them a promising tool in the treatment of PD. This review summarizes the current understanding of MSC-derived exosomes and their potential role in the cellular and molecular basis of PD, focusing on the involvement of miRNAs.
NEUROLOGICAL SCIENCES
(2023)
Article
Orthopedics
Madhan Jeyaraman, Sathish Muthu, Arun Gulati, Naveen Jeyaraman, G. S. Prajwal, Rashmi Jain
Summary: Researchers are continually developing new technologies for the treatment of knee osteoarthritis, including cell-based therapies and exosomal therapy. These new technologies can improve the quality of cartilage and alleviate symptomatic pain, potentially becoming the future direction for the management of knee osteoarthritis.
Article
Cardiac & Cardiovascular Systems
Rahele A. Farahani, Shasha Yu, Christopher M. Ferguson, Xiang-Yang Zhu, Hui Tang, Kyra L. Jordan, Ishran M. Saadiq, Sandra M. Herrmann, Alejandro R. Chade, Amir Lerman, Lilach O. Lerman, Alfonso Eirin
Summary: The study showed that PTRA treatment improved myocardial mitochondrial damage, enhanced microvascular maturity, reduced oxidative stress and fibrosis, and mitigated left ventricular remodeling and diastolic dysfunction in pigs with MetS and RVH, indicating the therapeutic potential of PTRA in these conditions.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH
(2022)
Review
Medicine, Research & Experimental
Samer H. Barsom, Logan M. Glasstetter, Sarosh Siddiqi, Kamalnath Sankaran Rajagopalan, Alfonso Eirin, Lilach O. Lerman
Summary: This review summarizes the evolving understanding of key pathophysiological mechanisms underlying chronic ischemic kidney injury and the development of new diagnostic and therapeutic approaches.
Article
Cell & Tissue Engineering
W. H. Trousdale, A. K. Limberg, N. Reina, C. G. Salib, R. Thaler, A. Dudakovic, D. J. Berry, M. E. Morrey, J. Sanchez-Sotelo, A. van Wijnen, M. P. Abdel
Summary: This study evaluated the intra-articular effects of celecoxib in treating arthrofibrosis, and found that intra-articular injections of celecoxib significantly improved passive extension angles and reduced capsular stiffness. Celecoxib was superior to surgical release, but the combination of celecoxib and surgical release did not provide additional benefits.
BONE & JOINT RESEARCH
(2022)
Review
Medicine, Research & Experimental
Maria Irazabal, Alejandro R. Chade, Alfonso Eirin
Summary: This review summarizes the involvement of renal mitochondrial injury and dysfunction in frequent forms of CKD, provides an overview of non-invasive biomarkers, and discusses potential mechanisms of renal mitochondrial damage.
Article
Genetics & Heredity
Ying Zhang, Ling Ling, Arya Ajay D. O. Ajayakumar, Yating Michelle Eio, Andre J. van Wijnen, Victor Nurcombe, Simon M. Cool
Summary: This study reveals a positive correlation between FGFR2 expression and osteogenic potential in human adult bone marrow-derived MSCs. Knockdown of FGFR2 leads to downregulation of pro-osteogenic genes and upregulation of pro-adipogenic genes, suppressing osteogenesis. Furthermore, FGFR2 knockdown results in upregulation of EZH2, an enzyme that regulates MSC lineage commitment and suppresses osteogenesis under osteogenic induction.
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2022)
Article
Multidisciplinary Sciences
Coralee E. Tye, Prachi N. Ghule, Jonathan A. R. Gordon, Fleur S. Kabala, Natalie A. Page, Michelle M. Falcone, Kirsten M. Tracy, Andre J. van Wijnen, Janet L. Stein, Jane B. Lian, Gary S. Stein
Summary: This study reveals a long non-coding RNA (MIR181A1HG) that is highly expressed in multipotent mesenchymal stromal cells (MSCs) and down-regulated during osteogenic differentiation. Knockdown of MIR181A1HG impedes cell cycle progression in MSCs and enhances their differentiation into osteo-chondroprogenitors. This study suggests that MIR181A1HG may serve as a regulator of new bone formation in skeletal disorders.
SCIENTIFIC REPORTS
(2022)
Article
Cardiac & Cardiovascular Systems
Alfonso Eirin, Alejandro R. Chade
Summary: Chronic kidney disease (CKD) is common in patients with heart failure and often results in left ventricular diastolic dysfunction (LVDD). Epigenetic alterations may play a role in cardiac abnormalities in CKD-LVDD, but their exact role is unknown. This study investigates whether changes in cardiac site-specific DNA methylation profiles are implicated in cardiac abnormalities in CKD-LVDD.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Genetics & Heredity
Parisa Dashti, Jeroen van de Peppel, Roman Thaler, Christopher R. Paradise, Gary S. Stein, Martin A. Montecino, Johannes P. T. M. van Leeuwen, Bram J. van der Eerden, Amel Dudakovic, Andre J. van Wijnen
Summary: This study found that Smyd2 inhibits proliferation and mineral deposition by osteoblasts, and operates parallel to the suppressive effects of Ezh2 and H3K27 trimethylation on osteoblast differentiation.
Editorial Material
Orthopedics
Andre J. van Wijnen, Eric A. Lewallen
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME
(2023)
Review
Biochemistry & Molecular Biology
Praneet Dara, Zeba Farooqui, Fackson Mwale, Chungyoul Choe, Andre J. van Wijnen, Hee-Jeong Im
Summary: Chronic pain conditions caused by arthritis have devastating effects on both individuals and society. The main objective of pharmacological treatments is to reduce pain, and several types of drugs have been considered. Naltrexone, an oral-activated opioid antagonist, has been shown to have beneficial effects on chronic pain conditions such as fibromyalgia and multiple sclerosis.
Article
Orthopedics
Hayman Lui, Cedryck Vaquette, Janet M. Denbeigh, Randy Bindra, Andre J. van Wijnen, Sanjeev Kakar
Summary: This study aims to assess the use of growth factors (BMP2 and GDF5) for bone and ligament regeneration in a multiphasic scaffold in a rabbit knee model. The addition of BMP2 and GDF5 in the BLB scaffold resulted in heterotopic bone formation and ligament failure.
JOURNAL OF WRIST SURGERY
(2023)
Article
Medicine, Research & Experimental
Sara Kazeminia, Xiang Y. Zhu, Hui Tang, Kyra L. Jordan, Ishran M. Saadiq, Sandra M. Herrmann, Alejandro R. Chade, Maria V. Irazabal, Lilach O. Lerman, Alfonso Eirin
Summary: This study found that renal artery stenosis (RAS) alters the expression of inflammatory genes and affects the DNA methylation and hydroxymethylation levels in scattered tubular-like cells (STCs) in pig kidneys. Some of the gene and protein expression normalized after epigenetic modulation. These findings provide insights into the regulatory mechanisms of the renal endogenous repair system and support the development of epigenetic or anti-inflammatory therapies for individuals with RAS.
Article
Endocrinology & Metabolism
Stephane Blouin, Farzaneh Khani, Phaedra Messmer, Paul Roschger, Markus A. Hartmann, Andre J. van Wijnen, Roman Thaler, Barbara M. Misof
Summary: Vitamin C is crucial for bone health and low levels of serum Vitamin C increase the risk of skeletal fractures. This study examined the effects of Vitamin C on femoral structure, bone formation, and bone mineralization density distribution in mice. The results showed that Vitamin C depletion significantly reduced cortical thickness and bone volume, as well as diminished the amount of newly formed bone tissue. Additionally, Vitamin C supplementation led to higher calcium concentrations and suggested a longer bone tissue age. Female mice were found to be more sensitive to Vitamin C deficiency. These findings highlight the key role of Vitamin C in bone formation rate and its potential in preventing bone-degenerative diseases, especially in females.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Siting Hong, Xiang-Yang Zhu, Yamei Jiang, Lei Zhang, Hui Tang, Kyra L. Jordan, Ishran M. Saadiq, Weijun Huang, Amir Lerman, Alfonso Eirin, Lilach O. Lerman
Summary: This study found that intrarenal delivery of autologous extracellular vesicles (EVs) can attenuate cardiac injury in experimental RVD more effectively than their parent mesenchymal stem cells (MSCs), including improving diastolic dysfunction and reducing myocardial fibrosis.
STEM CELL REVIEWS AND REPORTS
(2023)
