4.6 Article

Effect of Solvent Extraction and Blanching Pre-Treatment on Phytochemical, Antioxidant Properties, Enzyme Inactivation and Antibacterial Activities of 'Wonderful' Pomegranate Peel Extracts

Journal

PROCESSES
Volume 9, Issue 6, Pages -

Publisher

MDPI
DOI: 10.3390/pr9061012

Keywords

agriculture waste; antibacterial activity; antioxidant activity; blanching; peroxidase; polyphenol oxidase; polyphenols; pomegranate fruit peel; value-addition

Funding

  1. National Research Foundation of South Africa [64813]
  2. NRF [95442, 76555]

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The study found that ethanol at 70% concentration yielded the highest amount of extract with the best antioxidant activity, while blanching at 80 degrees Celsius for 3 minutes resulted in the extract with the highest antioxidant components. Ethanol extract showed better antioxidant activity than the other solvents tested, and blanching at 80 degrees Celsius for 3 minutes was the most effective in preserving the antioxidant properties of the pomegranate peel extract.
'Wonderful' pomegranate (Punica granatum L.) peel is rich in phytochemicals which are responsible for its strong antioxidant and antimicrobial activities, but it has low economic value as it is mainly discarded, causing an environmental waste management problem. To examine the best processing regime for pomegranate peel wastes, different solvents (ethanol, methanol and acetone) at various concentrations (50%, 70% and 100%) and blanching at 60, 80 and 100 degrees C for 1, 3 and 5 min, for each temperature, were tested. Ethanol at 70% (v/v) provided the highest extract yield, total phenolic and total tannin content at 29.46%, 10.61 +/- 0.15, and 0.76 +/- 0.02 mg GAE/g DM, respectively. Antioxidant activity using the 2,2 diphenyl-1-picryl hydrazyl assay (DPPH), ferric-reducing antioxidant power assay (FRAP) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid assay (ABTS) were reported at 243.97 +/- 2.43, 478.04 +/- 73.98 and 718.79 +/- 2.42 mu mol Trolox/g DM, respectively. A blanching temperature of 80 degrees C for 3 min led to the highest extract that had a total phenolic content of 12.22 +/- 0.08 mg GAE/g DM and total tannin content of 1.06 +/- 0.06 mg GAE/g DM. This extract also exhibited the best antioxidant activity for the DPPH, FRAP and ABTS assays. Two blanching temperatures, 80 or 100 degrees C, significantly reduced polyphenol oxidase and peroxidase activities (p < 0.05). Although blanched peel extracts showed a broad-spectrum activity against test bacteria, blanching at 80 degrees C for 3 or 5 min was most effective. Hot water blanching is thus a suitable environmentally friendly post-harvesting processing method for pomegranate peels that are intended for use as extracts in value-added products with good antioxidant and antibacterial effects.

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