Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

Three-dimensional bioprinting can fabricate precisely controlled 3D tissue constructs. This process uses bioinks-specially tailored materials that support the survival of incorporated cells-to produce tissue constructs. The properties of bioinks, such as stiffness and porosity, should mimic those found in desired tissues to support specialized cell types. Previous studies by our group validated soft substrates for neuronal cultures using neural cells derived from human-induced pluripotent stem cells (hiPSCs). It is important to confirm that these bioprinted tissues possess mechanical properties similar to native neural tissues. Here, we assessed the physical and mechanical properties of bioprinted constructs generated from our novel microsphere containing bioink. We measured the elastic moduli of bioprinted constructs with and without microspheres using a modified Hertz model. The storage and loss modulus, viscosity, and shear rates were also measured. Physical properties such as microstructure, porosity, swelling, and biodegradability were also analyzed. Our results showed that the elastic modulus of constructs with microspheres was 1032 +/- 59.7 Pascal (Pa), and without microspheres was 728 +/- 47.6 Pa. Mechanical strength and printability were significantly enhanced with the addition of microspheres. Thus, incorporating microspheres provides mechanical reinforcement, which indicates their suitability for future applications in neural tissue engineering.

Automated summary

Three-dimensional bioprinting allows the fabrication of precisely controlled 3D tissue constructs using specially tailored bioinks. Incorporating microspheres significantly enhances the mechanical strength and printability of bioprinted constructs, showing potential for future applications in neural tissue engineering.