Journal
ACTA NEUROPATHOLOGICA COMMUNICATIONS
Volume 9, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s40478-021-01228-0
Keywords
Nucleoporins; POM121; Nuclear pore complex; ALS; FTD; CHMP7; ESCRT-III; VPS4; CHMP2B; CHMP4B
Categories
Funding
- ALSA Milton Safenowitz Postdoctoral Fellowship
- NIH [K99NS123242]
- NIH-NINDS [NS099114, NS094239, AG057623]
- Department of Defense
- Robert Packard Center for ALS Research Answer ALS Program
- ALS Finding a Cure
- ALS Association
- Muscular Dystrophy Association
- F Prime
- Chan Zuckerberg Initiative
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Nuclear pore complex injury plays a significant role in ALS pathogenesis, with aberrant nuclear accumulation of the ESCRT-III protein CHMP7 being a key pathological event. While VPS4 expression is increased in ALS neuronal nuclei, CHMP4B and CHMP2B levels do not show a similar increase. Therapeutic efforts to mitigate this pathogenic cascade should focus on upstream events such as the nuclear accumulation of CHMP7.
Nuclear pore complex injury has recently emerged as an early and significant contributor to familial and sporadic ALS disease pathogenesis. However, the molecular events leading to this pathological phenomenon characterized by the reduction of specific nucleoporins from neuronal nuclear pore complexes remain largely unknown. This is due in part to a lack of knowledge regarding the biological pathways and proteins underlying nuclear pore complex homeostasis specifically in human neurons. We have recently uncovered that aberrant nuclear accumulation of the ESCRT-III protein CHMP7 initiates nuclear pore complex in familial and sporadic ALS neurons. In yeast and non-neuronal mammalian cells, nuclear relocalization of CHMP7 has been shown to recruit the ESCRT-III proteins CHMP4B, CHMP2B, and VPS4 to facilitate nuclear pore complex and nuclear envelope repair and homeostasis. Here, using super resolution structured illumination microscopy, we find that neither CHMP4B nor CHMP2B are increased in ALS neuronal nuclei. In contrast, VPS4 expression is significantly increased in ALS neuronal nuclei prior to the emergence of nuclear pore injury in a CHMP7 dependent manner. However, unlike our prior CHMP7 knockdown studies, impaired VPS4 function does not mitigate alterations to the NPC and the integral transmembrane nucleoporin POM121. Collectively our data suggest that while alterations in VPS4 subcellular localization appear to be coincident with nuclear pore complex injury, therapeutic efforts to mitigate this pathogenic cascade should be targeted towards upstream events such as the nuclear accumulation of CHMP7 as we have previously described.
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