Article
Biophysics
Faezeh Ghorbanizamani, Hichem Moulahoum, Figen Zihnioglu, Serap Evran, Candan Cicek, Ruchan Sertoz, Bilgin Arda, Tuncay Goksel, Kutsal Turhan, Suna Timur
Summary: The novel protein detection platform utilizes advanced technology with higher sensitivity, faster turnaround, and better stability compared to traditional nanoparticle methods. Smartphone-assisted sensing technology enables the tool to be used at home, reducing the burden on healthcare facilities.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Hichem Moulahoum, Faezeh Ghorbanizamani, Suna Timur
Summary: Synthetic cannabinoids, widely abused in modern society, have prompted the need for portable, efficient, and reliable on-site detection tools. This study introduces a colorimetric lateral flow assay combined with dye-loaded polymersome for efficient detection of the synthetic cannabinoid JWH-073, showing high sensitivity and selectivity. The method demonstrates promising applications in non-invasive substance abuse detection.
Article
Chemistry, Analytical
Kihyun Kim, Leila Kashefi-Kheyrabadi, Younju Joung, Kyeongnyeon Kim, Hajun Dang, Sachin Ganpat Chavan, Min-Ho Lee, Jaebum Choo
Summary: This review discusses the recent advances in SERS-based LFA platforms for the diagnosis of infectious diseases, emphasizing their high sensitivity and multiplex detection capability, as well as the advantages of combining them with LFAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Medicine, Research & Experimental
Tao Wang, Lanmei Chen, Arpitha Chikkanna, Suxiang Chen, Isabell Brusius, Nabayet Sbuh, Rakesh N. Veedu
Summary: This study analyzes key issues in the design of aptamer-based LFAs, including immobilization strategies, signaling methods, and target capturing approaches, providing a comprehensive overview of aptamer-based LFA design strategies to facilitate the development of optimized aptamer-based LFAs by researchers.
Article
Chemistry, Multidisciplinary
Haydn A. Little, Aysha Ali, Jake G. Carter, Matthew R. Hicks, Timothy R. Dafforn, James H. R. Tucker
Summary: A plug-and-play sandwich assay platform utilizing linear dichroism spectroscopy as a read-out method was developed for aptamer-based detection of molecular targets. The platform involved conjugating a 21-mer DNA strand onto the backbone of filamentous bacteriophage M13, generating a strong LD signal. Extended DNA strands containing thrombin-binding aptamer sequences were then bound to the linked DNA strand, creating aptamer-functionalized M13 bacteriophages. The sandwich sensor design showed effective detection of thrombin at pM levels, demonstrating the potential of this label-free assay system.
FRONTIERS IN CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Nicolas Moser, Ling-Shan Yu, Jesus Rodriguez Manzano, Kenny Malpartida-Cardenas, Anselm Au, Paul Arkell, Chiara Cicatiello, Ahmad Moniri, Luca Miglietta, Wen-Hung Wang, Sheng Fan Wang, Alison Holmes, Yen-Hsu Chen, Pantelis Georgiou
Summary: In this study, a smartphone-connected handheld Lab-on-Chip platform was translated for quantitative detection of dengue virus. The platform utilizes microchip technology and a reverse-transcriptase loop mediated isothermal amplification assay to achieve rapid and accurate detection. The platform communicates with a smartphone app and cloud server for real-time synchronization, enabling epidemiological surveillance. This technology shows promise for clinical diagnosis in low-resource settings.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biology
Elias Kabbas-Pinango, Moses Arinaitwe, Govert J. J. van Dam, Adriko Moses, Annet Namukuta, Andrina Barungi Nankasi, Nicholas Khayinja Mwima, Fred Besigye, Joaquin M. Prada, Poppy H. L. Lamberton
Summary: Schistosomiasis affects over 240 million people worldwide. Detecting the parasite eggs using Kato-Katz method is specific but not sensitive, while the urine-based POC-CCA test is more sensitive but has specificity issues and interpretation challenges. This study explores the variation in test results and suggests different thresholds for accurate diagnosis and prevalence monitoring.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Chemistry, Analytical
Milica Jovi, Denis Prim, Edis Saini, Marc Emil Pfeifer
Summary: Globally, 70 million people are affected by traumatic brain injury (TBI) annually. Diagnosis of mild TBI (mTBI) is challenging due to the absence of apparent symptoms. Current diagnostic methods rely on patient history, clinical aspects, and neuroimaging observations, which are costly and time-consuming. As an alternative, mTBI diagnostics can be done by detecting biomarkers in patients' blood. This study proposed two strategies for detecting mTBI biomarkers using electrochemiluminescence (ECL) immunoassay. Multiplex assays on a single disposable screen-printed carbon electrode (SPCE) were also developed. The methodology presented demonstrates the feasibility of a rapid point-of-care (POC) multiplex diagnostic system for mTBI biomarkers.
Article
Multidisciplinary Sciences
Seungmin Lee, Sunmok Kim, Dae Sung Yoon, Jeong Soo Park, Hyowon Woo, Dongho Lee, Sung-Yeon Cho, Chulmin Park, Yong Kyoung Yoo, Ki- Baek Lee, Jeong Hoon Lee
Summary: The authors present bioengineered enrichment tools for LFAs with enhanced sensitivity and specificity, which can improve the accuracy of LFA diagnosis. They also introduce a smartphone-based LFA diagnostics using deep learning-assisted algorithms, achieving high accuracy and reliability.
NATURE COMMUNICATIONS
(2023)
Article
Virology
Arlen-Celina Luecke, Anja vom Hemdt, Janett Wieseler, Carlo Fischer, Marie Feldmann, Simon Rothenfusser, Jan Felix Drexler, Beate Mareike Kuemmerer
Summary: A neutralization test based on wild-type single-round infectious virus replicon particles (VRPs) was established for high-throughput analysis of neutralizing flavivirus antibodies.
Article
Biophysics
Islam Seder, Rodrigo Coronel-Tellez, Seyed Hossein Helalat, Yi Sun
Summary: A chip has been developed for automated and sensitive purification, digitalization, and detection of SARS-CoV-2 viral RNA. The chip uses the surface charge concept of magnet bead-RNA binding to purify RNA and digitalizes the amplification mixture. It also offers a cost-effective method for fluorescent detection. This chip platform can be used for accurate and automated diagnosis of infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Daniel W. Bradbury, Jasmine T. Trinh, Milo J. Ryan, Kyle J. Chen, Adel A. Battikha, Benjamin M. Wu, Daniel T. Kamei
Summary: This study is the first to combine lateral-flow immunoassay with gold nanorod etching, achieving a multicolor readout that is correlated with digoxin concentrations in human serum, within the range of 0.5-3.0 ng mL(-1), for therapeutic drug monitoring.
Review
Food Science & Technology
Nadin Younes, Hadi M. Yassine, Katerina Kourentzi, Patrick Tang, Dmitri Litvinov, Richard C. Willson, Laith J. Abu-Raddad, Gheyath K. Nasrallah
Summary: The detrimental impact of foodborne pathogens on human health makes food safety a major concern. Conventional methods for detecting foodborne pathogens are tedious, time-consuming, laborious, and expensive. Lateral flow assays (LFAs) have gained attention for their rapid, cheap, and on-site pathogen detection capability. This article reviews the latest developments in LFAs for detecting foodborne pathogens and discusses improvements in performance and challenges for further development.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Chemistry, Analytical
Hyun Tae Kim, Enjian Jin, Min-Ho Lee
Summary: In this study, a portable chemiluminescence-based lateral flow assay platform was developed for the detection of cortisol in human serum. The platform showed high sensitivity and accuracy for measuring cortisol levels and could be a cost-effective alternative to optical devices.
Article
Chemistry, Analytical
Cecilie Knudsen, Jonas A. Jurgensen, Pelle D. Knudsen, Irina Oganesyan, Julian A. Harrison, Soren H. Dam, Aleksander M. Haack, Rasmus U. W. Friis, Lars Vitved, Selma B. Belfakir, Georgina M. S. Ross, Renato Zenobi, Andreas H. Laustsen
Summary: Brazil is home to venomous snakes, with Bothrops genus being medically relevant. Bothrops spp. account for 70% of all snakebites in Brazil, and their envenomings can be treated with three types of antivenom. This article introduces a prototype of a lateral flow assay (LFA) capable of detecting venom from Brazilian Bothrops spp. The LFA shows potential in diagnosing Bothrops envenomings and could improve snakebite management in the highly neglected disease.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Tugba Celiker, Faezeh Ghorbanizamani, Hichem Moulahoum, Emine Guler Celik, Kerem Tok, Figen Zihnioglu, Candan Cicek, Ruchan Sertoz, Bilgin Arda, Tuncay Goksel, Kutsal Turhan, Suna Timur, Yusuf Yagci
Summary: A rapid and easy immunofluorescence bioassay for SARS-CoV-2 detection was developed using a one-pot synthetic approach for the production of a graft fluorescent copolymer PPy-g-PCL. The assay showed a detection range of 6.03-8.7 LOG viral copy mL(-1) and a high correlation with RT-PCR data. This study provides new insights for simplified synthesis procedures of reporter molecules and their potential applications in sensing and diagnosis.
Article
Cell Biology
Hichem Moulahoum, Faezeh Ghorbanizamani, Zineb Khiari, Mohamed Toumi, Yasmina Benazzoug, Kerem Tok, Suna Timur, Figen Zihnioglu
Summary: This study investigated the mechanism of action of Artemisia herba-alba (AHA) and its effects on diabetes-related liver damages. The results showed that AHA inhibited protein modification and AGE formation, reduced inflammation, oxidative stress, and apoptosis, and regulated the signaling pathways involved in liver damages caused by AGEs. The findings suggest that AHA has potential as a multitarget option in the therapy of diabetes and AGEs-related diseases.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Zinar Pinar Gumus, Hichem Moulahoum, Kerem Tok, Ebru Kocadag Kocazorbaz, Figen Zihnioglu
Summary: This study aimed to purify and identify potential endogenous peptides of barley sprouts for potential type 2 diabetes (T2D) treatment. The findings showed that peptide fractions with molecular weights lower than 3 kDa were the only ones with antiglycation activities. Among them, F11 demonstrated the highest potential as a T2D treatment.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Review
Chemistry, Inorganic & Nuclear
Simge Balaban Hanoglu, Duygu Harmanci, Nursima Ucar, Serap Evran, Suna Timur
Summary: In recent years, magnetic nanoparticles (MNPs) have been widely used in various fields, including cancer detection, due to their beneficial properties. Detecting microRNAs (miRNAs) in biological samples is challenging, but MNPs provide an effective solution with their large surface areas and magnetic moments. This article discusses the synthesis and characterization methods of MNPs and their application in miRNA detection using biosensing systems, considering the challenges and prospects in terms of development stages, sensitivity, and selectivity.
Article
Chemistry, Analytical
Huseyin Furkan Kiyikci, Okan Avci, Yudum Tepeli Buyuksunetci, Suna Timur, Ulku Anik
Summary: A facile amperometric biosensor was developed using a Co/2Fe metal-organic framework (MOF) with oxidase mimicking properties for the detection of sialic acid (SA). The biosensor was constructed by immobilizing Co/2Fe MOF and N-acetylneuraminic Acid Aldolase (NANA-Aldolase) enzyme on a gold screen-printed electrode. The sensor showed a linear range of 0.02 mM-1.00 mM for SA concentration and a limit of detection of 0.026 mM. The developed biosensor was successfully applied for the detection of SA in GD3 ganglioside and HeLa cancer cell lines, providing a more practical and accurate system.
Review
Chemistry, Multidisciplinary
Faezeh Ghorbanizamani, Hichem Moulahoum, Emine Guler Celik, Suna Timur
Summary: The growing demand for personalized treatments and constant vital signs observation has led to the development of implantable biosensors that can temporarily or permanently be introduced into the human body. However, ensuring reliable functionality in the complex environment of the human body remains a critical challenge.
APPLIED SCIENCES-BASEL
(2023)
Review
Chemistry, Analytical
Bilge Asci Erkocyigit, Ozge Ozufuklar, Aysenur Yardim, Emine Guler Celik, Suna Timur
Summary: Microfluidics plays a crucial role in lab-on-a-chip systems, allowing for manipulation of fluid behavior and control on a small platform. Paper-based microfluidic analytical devices (muPADs) have numerous advantages, especially in cancer biomarker detection, and have the potential to improve diagnosis and treatment outcomes.
Review
Chemistry, Analytical
Faezeh Ghorbanizamani, Hichem Moulahoum, Emine Guler Celik, Figen Zihnioglu, Tutku Beduk, Tuncay Goksel, Kutsal Turhan, Suna Timur
Summary: Minimally invasive approaches in cancer diagnosis, such as liquid biopsies using blood samples, provide valuable information about the tumor and its state. However, isolating and detecting markers in complex matrices is challenging. Advances in polymer synthesis have led to enhanced platforms for liquid biopsies analysis, improving biosensor performance through increased sensitivity, improved binding, and avoidance of non-specific interactions. Additionally, polymer-based materials aid in signal amplification for low-concentration targets. This technology plays an essential role in oncology and clinical biomedicine, as shown by its critical application in liquid biopsies diagnosis.
Review
Nanoscience & Nanotechnology
Mohd. Rahil Hasan, Pradakshina Sharma, Shariq Suleman, Shouvik Mukherjee, Emine Guler Celik, Suna Timur, Roberto Pilloton, Jagriti Narang
Summary: Integrating electronic applications with paper can expand the uses of paper substrates, but putting electronics on paper is not easy. However, methods like building electronic devices on a rough, absorbent surface of the paper have made significant progress in paper electronics.
ACS APPLIED BIO MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Hichem Moulahoum, Faezeh Ghorbanizamani, Kerem Tok, Figen Zihnioglu
Summary: Peptides are important biomolecules with various applications. However, they have limitations such as flexible conformations and susceptibility to degradation. The discovery of cyclic peptides in plants has provided new insights for overcoming these issues.
Review
Nanoscience & Nanotechnology
Mohd Rahil Hasan, Pradakshina Sharma, Shariq Suleman, Shouvik Mukherjee, Emine Guler Celik, Suna Timur, Roberto Pilloton, Jagriti Narang
Summary: Integrating electronic applications with paper substrates can expand the potential uses of paper. While paper has advantages such as being disposable, inexpensive, and environmentally friendly, its rough and porous surface poses a challenge for putting electronics on paper. However, there are methods to make electronic devices compatible with the absorbent surface of paper.
ACS APPLIED BIO MATERIALS
(2023)
