Article
Plant Sciences
Carol Kalin, Agnese Kolodinska Brantestam, Anna-Kerstin Arvidsson, Mukesh Dubey, Malin Elfstrand, Magnus Karlsson
Summary: This study evaluated the resistance of six new back-crossed pea breeding lines derived from the cross between the susceptible commercial cultivar Linnea and the landrace PI180693 against Aphanomyces euteiches and Phytophthora pisi. The resistance of these lines was assessed in growth chamber, green house, and field trials. The results showed that the resistance from PI180693 interacted with the virulence levels of A. euteiches and was less effective against root rot caused by P. pisi. However, the breeding lines performed well in commercial production traits, indicating their potential use in commercial breeding programs.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Longfei Wu, Rudolph Fredua-Agyeman, Stephen E. Strelkov, Kan-Fa Chang, Sheau-Fang Hwang
Summary: In this study, novel genes associated with partial resistance to Aphanomyces root rot in field pea were identified using BSR-seq technology, providing a theoretical basis for the management of this disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Syama Chatterton, Timothy D. Schwinghamer, Antoine Page, Robyne Bowness Davidson, Michael W. Harding, Sabine Banniza
Summary: The presence of Aphanomyces euteiches causing root rot in pea and lentil crops in Saskatchewan and Alberta was first discovered in 2012 and 2013. Subsequent surveys from 2014 to 2017 showed that Aphanomyces root rot (ARR) was widespread across Canadian prairies. This study aimed to establish the relationship between oospore levels in different soil types and the severity of ARR, and to determine the correlation between measured DNA quantity of A. euteiches and the initial oospore dose in soils using droplet digital PCR or quantitative PCR. The findings of this research are important for developing a root rot risk assessment system for pulse crop production in Canadian prairies.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Laura Martin-Torrijos, Maria Martinez-Rios, Gloria Casabella-Herrero, Susan B. Adams, Colin R. Jackson, Javier Dieguez-Uribeondo
Summary: The study shows that the oomycete Aphanomyces astaci is widely distributed and genetically diverse in the southeastern US, supporting the hypothesis that the pathogen originated in this region. However, the pathogen exhibited no clear species-specificity or geographical patterns, in contrast to previous assumptions.
SCIENTIFIC REPORTS
(2021)
Article
Zoology
Maria Martinez-Rios, Laura Martin-Torrijos, Javier Dieguez-Uribeondo
Summary: The crayfish plague caused by Aphanomyces astaci poses a significant threat to freshwater crayfish populations worldwide. Despite advancements in molecular methods for identifying the pathogen, there is still a need to understand the resistance and tolerance immune mechanisms of the crayfish. This study aims to reproduce key developmental biology stages of A. astaci and identify crayfish populations with higher resistance and tolerance to the pathogen.
JOURNAL OF INVERTEBRATE PATHOLOGY
(2023)
Article
Microbiology
Xiao Lai, Dhirendra Niroula, Mary Burrows, Xiaogang Wu, Qing Yan
Summary: This study found that 2,4-diacetylphloroglucinol, an antibiotic produced by certain Pseudomonas species, plays an important role in the biocontrol of pea Aphanomyces root rot.
Article
Biochemistry & Molecular Biology
Yohana Laloum, Christophe Gangneux, Bruno Gugi, Arnaud Lanoue, Thibaut Munsch, Adrien Blum, Adrien Gauthier, Isabelle Trinsoutrot-Gattin, Isabelle Boulogne, Maite Vicre, Azeddine Driouich, Karine Laval, Marie-Laure Follet-Gueye
Summary: The study revealed that pea is more susceptible to Aphanomyces euteiches infection compared to faba bean. Pea root exudates showed attraction towards the pathogen, while faba bean exudates exhibited a repellent effect. The research suggests that early belowground defense plays a critical role in host susceptibility to root rot, opening new perspectives for disease management.
Article
Agronomy
Carol Kalin, Anna Berlin, Agnese Kolodinska Brantestam, Mukesh Dubey, Anna-Kerstin Arvidsson, Paul Riesinger, Malin Elfstrand, Magnus Karlsson
Summary: This study investigated the genetic diversity of the oomycete pathogen Aphanomyces euteiches, which causes root rot in pea plants, in different countries in Europe. The results showed that strains from Italy were genetically distinct from strains in other countries, and strains from Finland and the eastern parts of Sweden were significantly different from other populations. However, although there were genetic differences between strains, there was no clear correlation between genetic relatedness and virulence levels.
Article
Plant Sciences
Andrei Kiselev, Laurent Camborde, Laura Ossorio Carballo, Farnusch Kaschani, Markus Kaiser, Renier A. L. van der Hoorn, Elodie Gaulin
Summary: In order to successfully infect the host, phytopathogens have evolved various proteases to counteract plant defense mechanisms and survive in harsh environments. This study focused on identifying active secreted proteases from the legume root rott pathogen Aphanomyces euteiches. Through genome mining and expression analysis, an abundance of tandemly repeated proteases, which are upregulated during host infection, were discovered. Activity-based protein profiling and mass spectrometry on infected pea roots led to the identification of 35 active extracellular microbial proteases, accounting for about 30% of the expressed genes encoding serine and cysteine proteases during infection. Notably, eight of the identified active secreted proteases contain an additional C-terminal domain. This research elucidates the presence of novel active modular extracellular eukaryotic proteases that may contribute to the pathogenicity of Aphanomyces genus.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Ecology
Laura Martin-Torrijos, Angel Jose Correa-Villalona, Juan Carlos Azofeifa-Solano, Fresia Villalobos-Rojas, Ingo S. Wehrtmann, Javier Dieguez-Uribeondo
Summary: The crayfish plague pathogen Aphanomyces astaci is a main factor leading to the decline of European and Asian native crayfish species, spread through natural carriers introduced from North America. The red swamp crayfish Procambarus clarkii poses a significant threat to crayfish biodiversity and freshwater ecosystems globally. Detection of A. astaci in introduced populations of P. clarkii in Central America serves as a warning about the potential dangerous impacts of invasive crayfish species and the need for proper management to prevent further ecological damage.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2021)
Article
Biodiversity Conservation
Michaela Mojzisova, Jitka Svobodova, Eva Kozubikova-Balcarova, Eva Struncova, Robin Stift, Michal Bily, Antonin Kouba, Adam Petrusek
Summary: The presence of North American alien crayfish in Europe has led to declines in native crayfish populations due to their role as carriers of Aphanomyces astaci, the pathogen responsible for crayfish plague. In Czechia, screening for A. astaci revealed new hotspots of infected crayfish and the potential for interspecific transmission between different crayfish species. Despite fluctuations, A. astaci prevalence in North American host populations generally does not vary significantly over time, with some populations still posing a threat to native crayfish.
Review
Biochemistry & Molecular Biology
Ashebir T. Godebo, Naomi Marie J. Wee, Christopher K. Yost, Fran L. Walley, James J. Germida
Summary: The effectiveness of biocontrol agents in suppressing aphanomyces root rot was analyzed using a meta-analysis approach, which revealed a high overall effectiveness. Various factors such as application method, biocontrol agent type, and study type were found to have significant impacts on the effectiveness. Soil amendments, seed coating and suspension applications, bacterial and fungal biocontrol agents were associated with positive outcomes for aphanomyces root rot suppression.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Fisheries
Pavel Kozak, Kamile Gonca Erol, Oguz Yasar Uzunmehmetoglu, Michiel Tangerman, Michaela Mojzisova, Remziye Ozkok, Antonin Kouba, Sakir Cinar, Adam Petrusek
Summary: Crayfish plague, caused by Aphanomyces astaci, poses a threat to populations of endangered crayfish and affects fisheries and aquaculture. In Turkey, the narrow-clawed crayfish industry suffered from stock collapses due to accidental introduction of crayfish plague. The establishment of closed culturing systems faces challenges from crayfish plague symptoms development and substantial mortality.
Article
Chemistry, Analytical
L. G. Divyanth, Afef Marzougui, Maria Jose Gonzalez-Bernal, Rebecca J. McGee, Diego Rubiales, Sindhuja Sankaran
Summary: This study utilized deep learning algorithms to evaluate the resistance of pea roots to Aphanomyces root rot (ARR). By implementing three effective data-balancing techniques, the classification accuracy of the model was improved, showing the potential for application in other image-based phenotyping datasets.
Article
Agronomy
Zakir Hossain, Michelle Hubbard, Yantai Gan, Luke D. Bainard
Summary: The study revealed that root rot generally increased the diversity of the pea root-associated microbiome and altered the community structure and relative abundance of key taxa. Healthy samples were associated with a higher relative abundance of Rhizobium, Olpidium and Mortierella, while diseased samples had more indicator genera and taxa exclusively detected in the diseased core microbiome.
Article
Biodiversity Conservation
Clementine Preau, Romain Bertrand, Yann Sellier, Frederic Grandjean, Francis Isselin-Nondedeu
Summary: The study found that climate change is the primary driver of the future distribution range reduction of Triturus marmoratus in France, with land use/land cover change playing a secondary role. The species may not be able to track the northward shift of its suitable habitat rapidly enough due to its insufficient dispersal ability and habitat fragmentation caused by human activities.
ANIMAL CONSERVATION
(2022)
Article
Environmental Sciences
Thomas Baudry, Eric Gismondi, Jean-Pierre Gout, Alexandre Arque, Juliette Smith-Ravin, Frederic Grandjean
Summary: The redclaw crayfish was introduced to Martinique Island for aquaculture purposes in the 21st century, but due to chlordecone pollution in the rivers, there is a risk of contamination in the crayfish muscle which may pose a serious risk to consumers. The study highlighted the rapid and continuous bioconcentration of chlordecone in the crayfish muscle, even after 20 days of depuration in chlordecone-free water.
Article
Plant Sciences
Laurent Camborde, Andrei Kiselev, Michiel J. C. Pel, Aurelie Le Ru, Alain Jauneau, Cecile Pouzet, Bernard Dumas, Elodie Gaulin
Summary: This study reveals that the oomycete effector AeSSP1256 targets a plant RNA helicase MtRH10, impairs root development and defense, and promotes pathogen infection by affecting nucleolar function.
Article
Zoology
Michel Breuil, David Schikorski, Barbara Vuillaume, Ulrike Krauss, Jennifer C. Daltry, Glenroy Gaymes, Joanne Gaymes, Olivier Lepais, Nicolas Bech, Misel Jelic, Thomas Becking, Frederic Grandjean
Summary: The newly described horned iguana Iguana insularis from the southern Lesser Antilles is separated into two subspecies: I. insularis sanctaluciae from St. Lucia and I. insularis insularis from the Grenadines. The study used genetic and morphological analysis to complete the description and found high levels of introgression and hybridization with other Iguana species in some populations. This research is important for the conservation of native iguanas, as they face threats from invasive iguanas and require urgent conservation action.
Article
Genetics & Heredity
Christopher M. Austin, Laurence J. Croft, Frederic Grandjean, Han Ming Gan
Summary: The yabby genome was assembled using Nanopore and Illumina sequencing methods, resulting in the most contiguous freshwater crayfish genome assembly. An unusually large number of cellulase genes were found in the genome, which is relevant to understanding the nutrition and ecology of this species.
FRONTIERS IN GENETICS
(2022)
Article
Microbiology
Andrei Kiselev, Helene San Clemente, Laurent Camborde, Bernard Dumas, Elodie Gaulin
Summary: Aphanomyces euteiches, a soil-borne oomycete pathogen, causes root rot diseases in legumes. Different pathotypes of A. euteiches show varying virulence, with secreted proteases and CAZymes being key factors. The specific secretome of A. euteiches includes small, secreted proteins without predicted functional domains, potentially influencing its preference for legumes. This study lays the foundation for further research on the interactions between A. euteiches and legumes.
Article
Biodiversity Conservation
Elie Morin, Pierre-Alexis Herrault, Yvonnick Guinard, Frederic Grandjean, Nicolas Bech
Summary: Urban landscapes are rapidly changing ecosystems. Designing and modeling ecological networks is crucial for land management. Remote sensing can produce reliable land cover maps, improving connectivity indices and providing new insights into conservation planning when combined with fine-scale landscape connectivity modeling.
ECOLOGICAL INDICATORS
(2022)
Article
Biodiversity Conservation
Misel Jelic, Damjan Franjevic, Domagoj Dikic, Petra Korlevic, Matej Vucic, Dusan Jelic, Thomas Becking, Frederic Grandjean, Goran Klobucar
Summary: This study found that the Crna Mlaka fishponds in Central Croatia are at a high risk of being invaded by non-native water frog species, leading to introgressive hybridization and genetic admixture. The presence of hybrid forms and introgressed mtDNA indicates the existence of a unique hybrid swarm in this location. The loss of the hybridogenetic reproductive system of Pelophylax kl. esculentus is also observed.
BIOLOGICAL INVASIONS
(2022)
Article
Fisheries
Matej Vucic, Misel Jelic, Goran Klobucar, Dusan Jelic, Han Ming Gan, Chris Austin, Daniel Guyonnet, Isabelle Giraud, Thomas Becking, Frederic Grandjean
Summary: Minnows of the genus Phoxinus are a common fish species in Palearctic freshwater habitats. The taxonomic status and phylogenetic relationships of Phoxinus species are not well understood. The authors developed microsatellite markers to study genetic diversity and interbreeding in Phoxinus species, and found that these markers are suitable for detecting introgression and hybridization among populations.
JOURNAL OF FISH BIOLOGY
(2022)
Article
Biodiversity Conservation
Leona Lovrencic, Martina Temunovic, Lena Bonassin, Frederic Grandjean, Christopher M. Austin, Ivana Maguire
Summary: The stone crayfish Austropotamobius torrentium is globally endangered due to anthropogenic pressure, climate change, and the invasive signal crayfish Pacifastacus leniusculus. The study shows that climate change is likely to result in significant loss of suitable habitats for A. torrentium, putting highly diverse populations at risk of extinction. Furthermore, the threat from climate change appears to be greater for A. torrentium than the invasive crayfish species.
GLOBAL ECOLOGY AND CONSERVATION
(2022)
Article
Environmental Sciences
Thomas Baudry, Quentin Mauvisseau, Alexandre Arque, Jean-Pierre Gout, Carine Delaunay, Hugo J. de Boer, Frederic Grandjean
Summary: In this study, an eDNA detection protocol was developed to investigate the presence of Anablepsoides cryptocallus, the only endemic freshwater fish of Martinique. The presence of the species was confirmed in 27 sites using eDNA detection. eDNA-based detection was found to be more effective and less time-consuming than conventional fishing, making it a relevant tool for future studies.
AQUATIC CONSERVATION-MARINE AND FRESHWATER ECOSYSTEMS
(2023)
Article
Ecology
Ella Z. Daly, Olivier Chabrerie, Francois Massol, Benoit Facon, Manon C. M. Hess, Aurelie Tasiemski, Frederic Grandjean, Matthieu Chauvat, Frederique Viard, Estelle Forey, Laurent Folcher, Elise Buisson, Thomas Boivin, Sylvie Baltora-Rosset, Romain Ulmer, Patricia Gibert, Gabrielle Thiebaut, Jelena H. Pantel, Tina Heger, David M. Richardson, David Renault
Summary: With the increasing level of biological invasions in the Anthropocene, there is still significant debate and lack of clarity on the determinants of success, impact, and sustaining mechanisms of introduced species. Empirical studies show divergent impacts and effects of biotic and abiotic factors, hindering the creation of a unified theory. The proposed synthesis categorizes invasion hypotheses along a timeline, providing a framework to navigate the theories and select appropriate concepts based on the stage of invasion.
Review
Mycology
Edward Dearberg, Nathan Smith
Summary: This article discusses the application of photogrammetry in the field of mycology, highlighting the initial tests using dried fungal material and the improvements in methodology.
FUNGAL BIOLOGY REVIEWS
(2024)
