Article
Biotechnology & Applied Microbiology
Feiyan Zhao, Xiaoye Bai, Juntao Zhang, Lai-Yu Kwok, Lingling Shen, Hao Jin, Tiansong Sun, Zhihong Sun, Heping Zhang
Summary: Bifidobacteria are beneficial gut inhabitants and their responses to probiotic intervention vary depending on subjects' geographic origins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Applied
Mehdi Cherkaoui, Dominique Tessier, Virginie Lollier, Colette Larre, Chantal Brossard, Wieneke Dijk, Helene Rogniaux
Summary: This study aimed to explore the effect of heat treatment on the IgE binding capacity of ovalbumin (OVA). The results showed that both aggregation and glycation strongly impacted the IgE binding capacity. High-resolution mass spectrometry analysis revealed extensive cross-linking through disulfide and dehydroprotein bridges, as well as moderate but significant glycation. Furthermore, key amino acid residues implicated in the changes in IgE binding were identified.
Article
Biology
Feliznando Isidro Cardenas-Torres, Francisco Cabrera-Chavez, Aldo Alejandro Arvizu-Flores, Lilian Karem Flores-Mendoza, Veronica Lopez-Teros, Humberto Astiazaran-Garcia, Martina Hilda Gracia-Valenzuela, Oscar Gerardo Figueroa-Salcido, Jesus Gilberto Aramburo-Galvez, Noe Ontiveros
Summary: The mouse model is valuable for studying IgE responses to food proteins. Intraperitoneal administration of protein triggers more robust and consistent IgE responses compared to intragastric administration. This study provides scientific evidence for the development of a reproducible mouse model for evaluating food allergies.
Article
Biotechnology & Applied Microbiology
Vivian C. Miranda, Ramon O. Souza, Monica F. Quintanilha, Bruno Gallotti, Helder C. Assis, Ana Maria C. Faria, Jacques R. Nicoli, Denise C. Cara, Flaviano S. Martins
Summary: This study aimed to evaluate the probiotic and postbiotic potential of Akkermansia muciniphila BAA-835 in an ovalbumin food allergy model. The results showed that treatment with viable A. muciniphila could alleviate weight loss, decrease serum levels of allergy-related antibodies, reduce intestinal injury and inflammation, and modify the gut microbiota composition. Additionally, the administration of inactivated bacteria also had a postbiotic effect by reducing allergy-related immune responses. These findings suggest that A. muciniphila BAA-835 has both probiotic and postbiotic properties in the prevention and treatment of food allergies.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Biotechnology & Applied Microbiology
L. M. Baker, T. S. Webberley, G. Masetti, T. R. Hughes, J. R. Marchesi, A. A. Jack, T. S. C. Joyce, M. D. Allen, S. F. Plummer, D. R. Michael, G. Ramanathan, R. Del Sol, P. D. Facey
Summary: Genomic sequences of the Lab4 probiotic consortium were deposited in Genbank, confirming functionality and safety. Probiogenomic analyses showed good tolerance to bile and acid, with no significant risk of antibiotic resistance or virulence genes. Tolerance studies in rats further supported safety, indicating Lab4's survival through the gastrointestinal tract.
Article
Food Science & Technology
Lana S. Rosa, Mariana L. Santos, Joel P. Abreu, Ramon S. Rocha, Erick A. Esmerino, Monica Q. Freitas, Eliane T. Marsico, Pedro H. Campelo, Tatiana C. Pimentel, Marcia Cristina Silva, Adrieli A. Souza, Fabio C. S. Nogueira, Adriano G. Cruz, Anderson J. Teodoro
Summary: The effect of probiotic strains on fermented whey-milk beverages was evaluated during storage. The addition of probiotics increased α-amylase and α-glucosidase inhibition and antioxidant activities, with the best effect observed after 15 days of storage. L. casei-01 showed higher metabolic activity and the presence of anti-hypertensive peptides, while La-5 and Bb-12 showed improvements in alpha-glucosidase inhibition and the presence of peptides with various activities.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Food Science & Technology
Dalia G. Kamel, Ahmed R. A. Hammam, Khalid A. Alsaleem, Dina M. Osman
Summary: The study found that the addition of inulin and storage time significantly affected the titratable acidity of probiotic yogurt, with inulin enhancing the viability of Bifidobacterium bifidum and acting as an antimicrobial against molds.
FOOD SCIENCE & NUTRITION
(2021)
Article
Multidisciplinary Sciences
Maha Fahad Alenazy, Fatemeh Saheb Sharif-Askari, Mohammed S. El-Wetidy, Narjes Saheb Sharif-Askari, Ibrahim Yaseen Hachim, Mohammad-Hani Temsah, Basema Saddik, Roua Al-Kufaidy, Maha A. Omair, Yasser A. Alshawakir, Amany Adulgadel Fathaddin, Suad Hannawi, Qutayba Hamid, Mohammed A. Omair, Saleh Al-Muhsen, Rabih Halwani
Summary: Treating asthmatic mice model with abatacept, either intranasally or intraperitoneally, reduced lung inflammation. Intranasal treatment with abatacept increased the frequency of IL-35 and IL-10 producing Bregs in the lung tissues to a higher level compared to intraperitoneal treatment. Moreover, the frequency of lungs LAG3(+) Tregs was significantly increased following treatment, which was also associated with a reduction in lung tissue and serum IL-17 levels of treated mice.
Article
Food Science & Technology
Porntipha Vitheejongjaroen, Praphaiphan Kanthawang, Fabien Loison, Yamaratee Jaisin, Ulisa Pachekrepapol, Malai Taweechotipatr
Summary: This study confirmed the antioxidative properties of Bifidobacterium animalis MSMC83 derived from healthy human infants and successfully developed probiotic yoghurt with antioxidative effects. The probiotic yoghurt had a shorter fermentation time, firmer texture, and lower syneresis compared to yoghurt fermented with starter cultures, making it suitable for the development of antioxidant-rich dairy products.
Review
Microbiology
Jiayu Li, Junyu Wang, Meiyu Wang, Li Zheng, Qiuyu Cen, Fangfang Wang, Li Zhu, Rizhao Pang, Anren Zhang
Summary: Depression is a prevalent psychological disease that significantly impacts individuals, families, and society. With the increase in COVID-19 cases, depression rates have further risen globally. Probiotics, especially Bifidobacterium, have been shown to have positive effects in preventing and treating depression, potentially through anti-inflammatory processes and regulation of neurotransmitter synthesis and hormonal balance. This mini-review summarizes the relationship between Bifidobacterium and depression, highlighting the potential future role of Bifidobacterium-based preparations in depression prevention and treatment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Mari Enokida, Ken-ichiro Minato, Susumu Yoshino, Nobuaki Ohto, Hiroshige Kuwahara, Masashi Mizuno
Summary: Research on food components with anti-allergic effects has gained attention in recent years. LP22A3 has been found to significantly improve allergic responses by reducing IgE production and restoring colon temperature. Furthermore, LP22A3 suppresses Th2 differentiation and IL-4 production by downregulating OX40L, thereby suppressing IgE production. These findings suggest that LP22A3 could be a safe and effective treatment for allergic diseases.
Article
Food Science & Technology
Maritiele Naissinger da Silva, Bruna Lago Tagliapietra, Neila Silvia Pereira dos Santos Richards
Summary: The study aimed to develop new products by encapsulating Lactobacillus acidophilus and Bifidobacterium bifidum in butter, assessing the effects of different capsule concentrations on product acceptance. Results indicated that capsule concentrations of 5% and 10% yielded optimal product performance, with good acceptance and purchase intention.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Veterinary Sciences
Susan Ciaravolo, Lina Maria Martinez-Lopez, Richard J. N. Allcock, Andrew P. Woodward, Caroline Mansfield
Summary: This longitudinal microbiome study found that a commercially available veterinary synbiotic product did not significantly impact the microbial diversity of healthy dogs' fecal microbiome, but did result in transient increases in certain probiotic species, with the microbiota partially reverting to its baseline state after discontinuation of the product.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Review
Chemistry, Physical
Yue Jiang, Xueting Pan, Tao Yu, Hai Wang
Summary: The existence of the blood-brain barrier (BBB) poses challenges for drug delivery to the central nervous system (CNS). Intranasal administration has emerged as an alternative method for delivering drugs to the brain by bypassing the BBB. This review focuses on the mechanisms, properties, and strategies of intranasal nanosystems for brain-targeted drug delivery. Despite promising results in animal models and clinical applications, significant challenges need to be overcome for the translation of these nanosystems into clinics. The future prospects of intranasal drug delivery nanosystems are discussed, aiming to provide insights and guidance for effective neurological disease treatment.
Article
Food Science & Technology
Mitra Molaee Parvarei, Mohammad Reza Fazeli, Amir M. Mortazavian, Solmaz Sarem Nezhad, Seyed Ali Mortazavi, Ali Akbar Golabchifar, Nasim Khorshidian
Summary: The study found that incorporating paraprobiotics into yogurt can increase the lactic acid content and water-holding capacity, reduce syneresis, without affecting the color parameters of the yogurt.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Microbiology
Agnieszka Razim, Sabina Gorska, Andrzej Gamian
Summary: CD is a Gram-positive, anaerobic bacterium that primarily infects hospitalized and elderly individuals who have undergone long-term antibiotic therapy. The increasing elderly population and antibiotic usage suggest that CD infections will continue to rise. Currently, there is no vaccine available for CD. Clinical trials using toxin-based antigens have shown limited efficacy in preventing colonization and transmission between patients. Therefore, a CD vaccine should include antigens from the bacterium or its spores to effectively combat toxins and inhibit patient colonization.
Article
Immunology
Dagmar Srutkova, Hana Kozakova, Tereza Novotna, Sabina Gorska, Petra Petr Hermanova, Tomas Hudcovic, Tereza Svabova, Marek Sinkora, Martin Schwarzer
Summary: The high prevalence of allergic diseases in industrialized countries is believed to be related to changes in microbial exposure. The administration of exopolysaccharide (EPS) from Lacticaseibacillus (L.) rhamnosus LOCK900 induces immune responses in the lungs of mice, leading to a reduction in allergic airway inflammation and Th2 cytokine response in sensitized individuals. Furthermore, EPS treatment increases the production of IgA in the lungs of sensitized mice. These findings suggest that EPS derived from L. rhamnosus LOCK900 could be a safe candidate for preventing the development of allergic symptoms in sensitized individuals.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Marcelina Pyclik, Justyna Durslewicz, Joanna A. Papinska, Umesh S. Deshmukh, Harini Bagavant
Summary: Activation of the STING protein has paradoxical effects in skin disease, exacerbating psoriatic skin disease and delaying wound healing in diabetic mice, while facilitating wound healing in normal mice. Injection of a STING agonist induced severe inflammation in the skin, but the lesions were self-limiting and resolved within 6 weeks. Pre-treating mice with poly (I:C) increased systemic inflammatory responses and worsened inflammation with delayed wound resolution. Prior systemic inflammation amplifies STING-mediated inflammatory responses and skin disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Marek Sinkora, Miroslav Toman, Katerina Stepanova, Hana Stepanova, Lenka Leva, Jana Sinkorova, Romana Moutelikova, Jiri Salat, Dagmar Srutkova, Martin Schwarzer, Simon Sinkora, Helena Kupcova Skalnikova, Katerina Nechvatalova, Tomas Hudcovic, Petra Hermanova, Sarka Pfeiferova, Mirka Kratochvilova, Lenka Kavanova, Blanka Dusankova, Marek Sinkora
Summary: PRRSV invades the thymus and alters the immune response, resulting in chronic infection. Infected pigs produce antibodies that are unable to neutralize the virus, leading to the inability of the immune system to eliminate the virus.
MICROBES AND INFECTION
(2023)
Article
Multidisciplinary Sciences
Martin Schwarzer, Umesh Kumar Gautam, Kassem Makki, Anne Lambert, Tomas Brabec, Amelie Joly, Dagmar Srutkova, Pierre Poinsot, Tereza Novotna, Stephanie Geoffroy, Pascal Courtin, Petra Petr Hermanova, Renata C. Matos, Jonathan J. M. Landry, Celine Gerard, Anne-Laure Bulteau, Tomas Hudcovic, Hana Kozakova, Dominik Filipp, Marie -Pierre Chapot-Chartier, Marek Sinkora, Noel Peretti, Ivo Gomperts Boneca, Mathias Chamaillard, Hubert Vidal, Filipe De Vadder, Francois Leulier
Summary: The intestinal microbiota plays a role in postnatal growth, and Lactiplantibacillus plantarum strain LpWJL has been found to support growth in undernourished animals. This strain stimulates insulin-like growth factor-1 (IGF-1) and insulin production and activity. Cell walls from LpWJL, as well as specific molecules, can promote growth despite undernutrition. NOD2 in intestinal epithelial cells is necessary for LpWJL-mediated IGF-1 production and growth promotion in malnourished animals.
Article
Chemistry, Applied
Katarzyna Pacyga-Prus, Dominika Jakubczyk, Corine Sandstrom, Dagmar Srutkova, Marcelina Joanna Pyclik, Katarzyna Leszczynska, Jaroskaw Ciekot, Agnieszka Razim, Martin Schwarzer, Sabina Gorska
Summary: Bifidobacteria are commonly used probiotics with well-described effects on the immune system. Recently, there is growing interest in biologically active molecules derived from bacteria. We characterized surface antigens of Bifidobacterium adolescentis CCDM 368 and found that Bad368.1 polysaccharide modulates cytokine production, can be transferred between cells, and may have potential in allergic disease modulation.
CARBOHYDRATE POLYMERS
(2023)
Article
Immunology
Tomas Brabec, Martin Schwarzer, Katarina Kovacova, Martina Dobesova, Dagmar Schierova, Jiri Brezina, Iva Pacakova, Dagmar Srutkova, Osher Ben-Nun, Yael Goldfarb, Iva Splichalova, Michal Kolar, Jakub Abramson, Dominik Filipp, Jan Dobes
Summary: This study identifies the cellular network that regulates MHCII expression on intestinal epithelial cells in response to SFB and demonstrates that SFB can drive the conversion of specific CD4(+) T cells to CD8 alpha(+) intraepithelial lymphocytes, leading to their accumulation in the small intestinal intraepithelial space. This mechanism is crucial for the SFB-induced increase in epithelial cell turnover.
JOURNAL OF EXPERIMENTAL MEDICINE
(2023)