Journal
FRONTIERS IN PHYSIOLOGY
Volume 12, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fphys.2021.676265
Keywords
skeletal muscle; mitochondrial dysfunction; high-fat diet; obesity; microRNA
Categories
Funding
- Sao Paulo Research Foundation (FAPESP) [20/08049-2, 18/07087-8, 15/24789-8, 11/05876-6]
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES) [001]
- FAPESP [2018/07241-7, 2017/19513-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [15/24789-8, 20/08049-2, 11/05876-6, 18/07087-8] Funding Source: FAPESP
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The study demonstrated that miR-1 precursor replacement therapy in muscle can improve insulin sensitivity in high-fat diet-fed mice, but cannot restore insulin sensitivity in cells in vitro. Overexpression of miR-1 may play a role in regulating mitochondrial respiration and the expression of genes related to fatty acid oxidation.
The role of microRNAs in metabolic diseases has been recognized and modulation of them could be a promising strategy to treat obesity and obesity-related diseases. The major purpose of this study was to test the hypothesis that intramuscular miR-1 precursor replacement therapy could improve metabolic parameters of mice fed a high-fat diet. To this end, we first injected miR-1 precursor intramuscularly in high-fat diet-fed mice and evaluated glucose tolerance, insulin sensitivity, and adiposity. miR-1-treated mice did not lose weight but had improved insulin sensitivity measured by insulin tolerance test. Next, using an in vitro model of insulin resistance by treating C2C12 cells with palmitic acid (PA), we overexpressed miR-1 and measured p-Akt content and the transcription levels of a protein related to fatty acid oxidation. We found that miR-1 could not restore insulin sensitivity in C2C12 cells, as indicated by p-Akt levels and that miR-1 increased expression of Pgc1a and Cpt1b in PA-treated cells, suggesting a possible role of miR-1 in mitochondrial respiration. Finally, we analyzed mitochondrial oxygen consumption in primary skeletal muscle cells treated with PA and transfected with or without miR-1 mimic. PA-treated cells showed reduced basal respiration, oxygen consumption rate-linked ATP production, maximal and spare capacity, and miR-1 overexpression could prevent impairments in mitochondrial respiration. Our data suggest a role of miR-1 in systemic insulin sensitivity and a new function of miR-1 in regulating mitochondrial respiration in skeletal muscle.
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