Integrated Utilization of Dairy Whey in Probiotic β-Galactosidase Production and Enzymatic Synthesis of Galacto-Oligosaccharides

This work established an integrated utilization of dairy whey in beta-galactosidase production from Lactobacillus bulgaricus and prebiotics synthesis by the probiotic enzyme. A cost-effective whey-based medium was newly developed for culturing Lactobacillus bulgaricus to produce beta-galactosidase. The medium was optimized through response surface methodology (RSM) involving a series of statistical designs, such as the Plackett-Burman design, steepest ascent experiment, and central composite design. Under the optimized medium, the beta-galactosidase activity of L. bulgaricus reached 2034 U/L, which was twice that produced from the traditional MRS medium. The cells of L. bulgaricus harvested from the whey-based medium were subsequently treated with lysozyme. The resulting crude enzyme was used as an efficient catalyst, which catalyzed the synthesis of the prebiotic galacto-oligosaccharides (GOS) in a high yield of 44.7% by using whey (200 g/L) as the substrate. The sugar mixture was further purified by activated charcoal adsorption, thereby yielding a high-purity level of 77.6% GOS.

Automated summary

The study established an integrated system for utilizing dairy whey in beta-galactosidase production and prebiotics synthesis, optimizing the medium for high yield and purity of GOS synthesis.