Article
Biochemical Research Methods
Chisato Takahashi, Mari Sato, Chikara Sato
Summary: Staphylococcus epidermidis, a gram-positive bacterium, plays an important role in biofilm formation and microvesicle secretion. This study successfully imaged the microvesicles secreted by Staphylococcus epidermidis and their integration into biofilms using atmospheric scanning electron microscopy (ASEM). The information obtained is expected to help elucidate the biological mechanism of biofilm formation and develop medicines against biofilms and associated infections.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Denis Swolana, Malgorzata Kepa, Celina Kruszniewska-Rajs, Robert D. Wojtyczka
Summary: Nanosilver has an impact on biofilm formation, gene expression, and viability of Staphylococcus epidermidis. Low concentrations of nanosilver may increase biofilm production, while high concentrations do not have this effect.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Bingjie Wang, Qing Zhan, Yanghua Xiao, Yanlei Xu, Huilin Zhao, Lulin Rao, Xinyi Wang, Jiao Zhang, Li Shen, Ying Zhou, Yinjuan Guo, Xiaocui Wu, Jingyi Yu, Fangyou Yu
Summary: Mupirocin stimulates the biofilm formation of Staphylococcus epidermidis by accelerating autolysis and eDNA release, depending on the specific gene atlE.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Chemistry, Physical
Qiuping Zhang, Jingni Wang, Fu Lan, Honglan Zhai, Fengmin Li, Tuo Ma, Danrong Li, Huaxin Hou
Summary: This study aimed to enhance the membrane permeability of cancer cells by introducing alpha-amino phosphate on the anthraquinone scaffold. 13 aloe emodin alpha-amino phosphate derivatives were synthesized, and compounds 2a and 2b showed better anti-tumor activity compared to aloe emodin. The interaction between compounds 2a, 2b, and DNA was studied, revealing that they interacted with DNA through intercalation. Furthermore, confocal microscopy and comet assay confirmed the effective penetration of 2a and 2b into the nucleus and increased DNA fragmentation in cancer cells. The alpha-amino phosphate-anthraquinone scaffold emerges as a promising structure for anticancer activity.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Biotechnology & Applied Microbiology
Stefana Dukanovi, Tea Ganic, Branka Loncarevic, Stefana Cvetkovic, Biljana Nikolic, Dina Tenji, Danijela Randjelovic, Dragana Mitic-Culafic
Summary: This study demonstrated the novel antibiofilm activity of emodin against Staphylococcus aureus, with strong antibacterial effects and inhibition of biofilm formation. Emodin was also found to affect biofilm structure, respiration, and gene expression, indicating multiple pathways of action against the pathogen.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andrej Minich, Zdenko Levarski, Maria Mikulasova, Marek Straka, Adriana Liptakova, Stanislav Stuchlik
Summary: The study showed that natural phenolic compounds vanillin and syringic acid have similar biofilm and extracellular polymer substance (EPS) inhibition effects on different genotypes of methicillin-resistant S. epidermidis strains. They effectively reduce biofilm formation and EPS content, making them potential inhibitors of biofilms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Xueqing Tang, Yue Zhang, Xin Liu, Xiaohan Li, Hongrui Zhao, Hao Cui, Yang Shi, Yongchao Chen, Honglin Xu, Ziyu Meng, Limin Zhao, Hui Chen, Zhixia Wang, Mengying Zhu, Yuan Lin, Baofeng Yang, Yong Zhang
Summary: Atherosclerosis is a highly lethal inflammatory disease associated with endothelial dysfunction. A study found that aloe-emodin derivative (AED) can reduce the development and progression of atherosclerosis by enhancing endothelial cell autophagy.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Martyna Nowak-Perlak, Mariusz A. Bromke, Piotr Ziolkowski, Marta Wozniak
Summary: This study investigated the potential of emodin and aloe-emodin as photosensitizers in photodynamic therapy for skin cancer. The results showed that these two substances can affect skin cancer cells and normal skin cells in different ways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Yixin Jin, Qichen Wang, Haomin Zhang, Na Zhao, Ziyu Yang, Hua Wang, Min Li, Qian Liu
Summary: Staphylococcus epidermidis, a human commensal, can form biofilm and cause invasive infections. Our study found that S. epidermidis isolates from different infection sites exhibited different biofilm forming capacity. Isolates from sterile body fluid showed thicker biofilm and expressed higher levels of accumulation-associated protein. The increased expression of certain proteins may contribute to the dispersal of bacteria from biofilm and subsequent spread.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Shihua Lan, Xiaofeng Chen, Chuanping Yin, Shengjun Xie, Shuaishuai Wang, Rongrong Deng, Zhibin Shen
Summary: Research found that Disaspidin BB obtained from plant extracts and purifications has inhibitory effect on drug-resistant Staphylococcus epidermidis biofilms, and its mechanism of anti-biofilm activity was preliminarily investigated.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Cell Biology
Kidon Sung, Miseon Park, Jungwhan Chon, Ohgew Kweon, Saeed A. Khan, Andrew Shen, Angel Paredes
Summary: This study investigates the effect of subinhibitory concentrations of tigecycline on biofilms formed by Staphylococcus epidermidis strain RP62A. The results show that different concentrations of tigecycline can cause changes in biofilm production and significantly affect protein expression. The study suggests that analyzing the global protein expression profile of biofilm cells under antibiotic pressure can improve our understanding of the mechanisms of antibiotic resistance.
Article
Microbiology
Yongqi Mu, Hong Zeng, Wei Chen
Summary: Quercetin exhibits anti-biofilm activity against Staphylococcus epidermidis by reducing cell surface hydrophobicity and inhibiting polysaccharide intercellular adhesin production, indicating its potential as an anti-biofilm agent for biofilm infections caused by S. epidermidis and potentially other bacteria.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Jingqi Zhao, Yantong Sun, Li Ren, Shuqing Huang, Jie Zhang
Summary: Aloe-emodin, a novel androgen receptor (AR) antagonist, has been identified to inhibit AR signaling by promoting apoptosis, modulating AR protein levels, inhibiting AR downstream target genes, and preventing the nuclear translocation of AR. These findings suggest that aloe-emodin may play a crucial role in prostate cancer prevention and treatment.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Debora de Souza Collares Maia Castelo-Branco, Gessica dos Santos Araujo, Xhaulla Maria Quariguasi Cunha Fonseca, Glaucia Morgana de Melo Guedes, Maria Gleiciane da Rocha, Raimunda Samia Nogueira Brilhante, Rossana de Aguiar Cordeiro, Jose Julio Costa Sidrim, Waldemiro Aquino Pereira-Neto, Marcos Fabio Gadelha Rocha
Summary: Aloe emodin and barbaloin were effective against both growing and mature biofilms, significantly reducing metabolic activity and biomass. Chrysophanol, on the other hand, only inhibited mature biofilms to a lesser extent.
Article
Microbiology
Ranjith Konduri, Chinthala Reddy Saiabhilash, Sisinthy Shivaji
Summary: The study revealed that Staphylococcus aureus and Staphylococcus epidermidis isolated from patients with Endophthalmitis have the potential to form biofilms, with faster formation on corneas and dispersal phase occurring within 48 hours. Biofilm formation renders the bacteria several-fold more resistant to antibiotics compared to planktonic cells.
Article
Microbiology
Zhimin Cheng, Zichen Li, Yi Yin, Zhengmin Lian, Hosny Ahmed Abdelgawad, Hai Hu, Xiang Guan, Dong Zuo, Yumei Cai, Chan Ding, Shaohui Wang, Tao Li, Jingjing Qi, Mingxing Tian, Shengqing Yu
Summary: The A19 strain of Brucella vaccine shows effective attenuation of virulence and reduced invasiveness in host cells, providing a potential strategy for controlling brucellosis.
VETERINARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Yanan Zhao, Lingli Liu, Shaohui Wang, Mingxing Tian, Jingjing Qi, Tao Li, Shengqing Yu
Summary: This study reported the draft genome sequence of a multidrug-resistant Klebsiella quasipneumoniae subsp. similipneumoniae strain isolated from a pig farm in China, revealing the presence of 12 important antibiotic resistance genes in its genome. The findings will be valuable for understanding antibiotic resistance and pathogenic mechanisms in Klebsiella quasipneumoniae.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Immunology
Ruiting Shen, Peng Yin, Hua Yao, Lu Chen, Xinyu Chang, Huanrong Li, Xiaolin Hou
Summary: The study revealed that Punicalin can inhibit LPS/ATP-induced pyroptosis by downregulating the ROS/NLRP3 inflammasome signaling pathway.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Chemistry, Organic
Tuanli Yao, Jiazhe Hui, Tao Liu, Tao Li
Summary: A palladium-catalyzed multicomponent reaction has been developed for the synthesis of o-alkylated arylacrylates and stilbenes through tandem intramolecular Heck cyclization/aryne dicarbofunctionalization, providing a straightforward method for accessing these compounds.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Veterinary Sciences
Chenglin Tao, Zhengfei Yi, Yaodong Zhang, Yao Wang, Hong Zhu, Dosseh Jean Apotre Afayibo, Tao Li, Mingxing Tian, Jingjing Qi, Chan Ding, Song Gao, Shaohui Wang, Shengqing Yu
Summary: Improper use of antibiotics has led to the rise of multidrug-resistant bacteria, posing a major health threat. Bacterial biofilms also contribute to antibiotic resistance. Phage therapy with SHWT1, a lytic phage against multidrug-resistant Salmonella, showed promising results in vitro and in vivo, suggesting its potential as a therapeutic agent against salmonellosis caused by resistant strains.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Applied
Jinjin Tong, Xiaolin Hou, Defeng Cui, Wu Chen, Hua Yao, Benhai Xiong, Lirong Cai, Hua Zhang, Linshu Jiang
Summary: This study prepared a novel berberine hydrochloride-carboxymethyl chitosan hydrogel (BH-CMCH) with excellent properties and demonstrated its antibacterial activity against Staphylococcus aureus in a rat mastitis model. Proteomics analysis indicated that BH-CMCH enhanced autolysosome degradation and activated the lysosomal pathway to promote the innate immune response.
CARBOHYDRATE POLYMERS
(2022)
Article
Immunology
Xingyun Liu, Xinyu Chang, Qin Wu, Jun Xu, Lu Chen, Ruiting Shen, Xiaolin Hou
Summary: Lithium chloride has been found to have antiviral activity against avian infectious bronchitis virus and can regulate inflammation response and cell apoptosis by affecting the expression of related genes. Clinical trials have shown that lithium chloride can reduce mortality and deformity rate in chicken embryos.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Jingjing Qi, Yu Wang, Haoran Li, Yuanbing Shang, Song Gao, Chan Ding, Xiaohan Liu, Shaohui Wang, Tao Li, Mingxing Tian, Shengqing Yu
Summary: Mycoplasma synoviae infection causes significant economic losses to the poultry industry. The enzyme MSPdhD is not only involved in energy metabolism and redox balance, but also plays a role in cytoadherence and pathogenesis of M. synoviae. Rabbit anti-rMSPdhD serum has a strong bactericidal activity and can inhibit the adhesion of M. synoviae to host cells.
VETERINARY MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Kangwen Xu, Limin Wang, Dan Xiong, Hongjun Chen, Xinru Tong, Xiaolong Shao, Tao Li, Guoliang Qian
Summary: The Wsp and DSF systems are two important signaling systems that mediate bacterial communications, and they control biofilm formation through regulating c-di-GMP. The Wsp system regulates biofilm formation via WspR-mediated c-di-GMP signaling, while the DSF system does not depend on RpfG's enzymatic activity. The WspR-RpfG complex acts as a molecular linker connecting the Wsp and DSF systems, enabling the Wsp system to dynamically integrate the DSF system and regulate biofilm formation.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Chemistry, Multidisciplinary
Tuanli Yao, Shuaijing Zhao, Tao Liu, Yuting Wu, Yanhui Ma, Tao Li, Xiangyang Qin
Summary: The first transition-metal-free regioselective synthesis of 2,3-diarylindenones via tandem annulation of 2-alkynylbenzaldehydes with phenols is reported. Two different reaction modes controlled by electronic effects and temperature can selectively produce either non-rearranged or rearranged indenones.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Jinxing Liao, Danyu Shen, Long Lin, Hongjun Chen, Yajie Jin, Shan-Ho Chou, Xiao-Quan Yu, Tao Li, Guoliang Qian
Summary: This study presents a new quorum-quenching strategy to inhibit bacterial infections by directly acquiring bacterial genes/proteins to block AHL signaling. By artificially expressing a typical AHL synthase gene in an antifungal bacterium, effective proteins that inhibit AHL synthesis were identified, leading to the discovery of a novel quorum-quenching protein, LqqP.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Veterinary Sciences
Yaodong Zhang, Yao Wang, Hong Zhu, Zhengfei Yi, Dosseh Jean Apotre Afayibo, Chenglin Tao, Tao Li, Mingxing Tian, Jingjing Qi, Chan Ding, Shengqing Yu, Shaohui Wang
Summary: The inactivation of the DctR gene in APEC resulted in decreased biofilm formation, reduced adherence capacity, and other negative effects, which were restored through genetic complementation. Additionally, DctR positively regulates the expression of ETT2 in APEC, influencing the virulence of the pathogen.
VETERINARY RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Tuanli Yao, Bo Wang, Beige Ren, Xiangyang Qin, Tao Li
Summary: The first palladium-catalyzed Ugi-type multicomponent reaction for the synthesis of N-acyl anthranilamides has been developed, providing efficient access to structurally diverse compounds from readily available starting materials. The utility of this method has been demonstrated by late stage functionalization of two commercial drugs: Flurbiprofen and Loxoprofen.
CHEMICAL COMMUNICATIONS
(2021)