Article
Immunology
Francesca Falconi-Agapito, Karen Kerkhof, Xiomara Merino, Diana Bakokimi, Fiorella Torres, Marjan Van Esbroeck, Michael Talledo, Kevin K. Arien
Summary: This study evaluated the discriminatory potential of 20 peptides identified using a flavivirus proteome-wide microarray and established a multiplex peptide immunoassay for detecting dengue virus infection. The findings have significant implications for the management of cases in a world with increasing exposure to arthropod-borne flaviviruses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Virology
Nurfatihah Zulkifli, Jasmine-Elanie Khairat, Adzzie-Shazleen Azman, Nur-Faralyza Mohd Baharudin, Nurul-Adila Malek, Syafiq-Asnawi Zainal Abidin, Sazaly Abubakar, Pouya Hassandarvish
Summary: This study investigated the antiviral properties of Streptomyces sp. KSF 103 methanolic extract (Streptomyces KSF 103 ME) against dengue virus serotype 2 (DENV-2). The extract showed maximum inhibitory properties during the virus entry stage. Minimal antiviral activities were observed at other virus replication stages. Further studies are needed to understand the antiviral mechanisms of Streptomyces KSF 103 ME against DENV.
Article
Virology
Caleb J. Stica, Roberto A. Barrero, Rachael Z. Murray, Gregor J. Devine, Matthew J. Phillips, Francesca D. Frentiu
Summary: Dengue is a global arboviral disease with high mortality rates. This study investigated trends in dengue virus (DENV) evolution using phylogenetic and selection pressure analyses. The results revealed important amino acid sites undergoing positive selection and genotypes diversifying at different rates. These findings enhance our understanding of DENV evolution and have implications for disease control and vaccine design.
Article
Infectious Diseases
Jaime E. Castellanos, Nayeli Jaimes, Carolina Coronel-Ruiza, Juan Pablo Rojas, Luis F. Mejia, Victor H. Villarreal, Luz E. Maya, Luz M. Claros, Cielo Orjuela, Eliana Calvo, Maria Victoria Munoz, Myriam L. Velandia-Romero
Summary: A study conducted in a pediatric clinic in Cali, Colombia, identified CHIKV as the arbovirus causing febrile cases in children, potentially leading to double infections. The results emphasize the importance of clinical tests to determine the arbovirus involved in infectious diseases for better treatment and surveillance.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Pablo Cantalice Santos Farias, Andre Filipe Pastor, Juliana Prado Goncales, Iasmyn Dayanne Santos do Nascimento, Ellida Suellen de Souza Ferraz, Thaisa Regina Rocha Lopes, Rodrigo Feliciano do Carmo, Maria Rosangela Cunha Duarte Coelho, Jose Valter Joaquim Silva Junior
Summary: This study was conducted in Recife, Pernambuco state, Brazil to investigate the influence of chikungunya (CHIKV) and Zika (ZIKV) viruses on the epidemiological profile of dengue cases. The results showed that although females were the main population diagnosed with dengue, chikungunya, and Zika infections, there were important differences in the age distribution of these viruses, which should be considered in public health policies and future studies of virus-host interaction.
BMC INFECTIOUS DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Katsuma Hayashi, Marie Fujimoto, Hiroshi Nishiura
Summary: This study quantitatively assessed the future risk of dengue in Japan using climate change scenarios and found that the risk of transmission may extend to late spring and autumn due to increasing temperatures. The study emphasizes the importance of developing adaptation policies to prevent dengue transmission, such as eliminating mosquito breeding sites and distributing insecticides.
FRONTIERS IN PUBLIC HEALTH
(2022)
Review
Virology
Kathryn M. Edenborough, Heather A. Flores, Cameron P. Simmons, Johanna E. Fraser
Summary: The introduction of Wolbachia strains into Aedes aegypti mosquito populations has been shown to significantly reduce dengue incidence, although RNA viruses are known to adapt to antiviral pressures. Long-term surveillance for resistant viruses should be an essential component of Wolbachia-introgression biocontrol programs.
JOURNAL OF VIROLOGY
(2021)
Article
Entomology
Marina E. O. Rangel, Luana P. R. Oliveira, Aline D. Cabral, Katharyna C. Gois, Marcos V. M. Lima, Beatriz C. A. A. Reis, Fernando L. A. Fonseca, Marcia A. Speranca, Flavia S. Gehrke, Gabriel Z. Laporta
Summary: The study screened Aedes mosquitoes in an urban area in southeastern Brazil and detected DENV-2 RNA in Aedes albopictus and Guadeloupe mosquito virus RNA in Aedes aegypti. The findings suggest that DENV-2 may have high infection rates and be endemic in the urban areas of Santo Andre, Brazil.
Article
Biotechnology & Applied Microbiology
Fabrizio Balestrino, Jeremy Bouyer, Marc J. B. Vreysen, Eva Veronesi
Summary: Effective control strategies, such as the sterile insect technique, play a crucial role in preventing the spread of vector-borne diseases. However, current sex separation techniques for mosquito vectors are not completely effective, resulting in increased risk of disease transmission. This study examined the transmission of dengue and chikungunya viruses in two Aedes species exposed to irradiation, and found that irradiation did not impact transmission efficiency.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Ecology
Nicole Nova, Ethan R. Deyle, Marta S. Shocket, Andrew J. MacDonald, Marissa L. Childs, Martin Rypdal, George Sugihara, Erin A. Mordecai
Summary: Experiments and models show that climate affects mosquito-borne disease transmission, with temperature and rainfall having net positive and negative effects respectively when susceptible availability is high. This study uses empirical dynamic modeling to effectively capture nonlinear and context-dependent effects of population susceptibility, temperature, and rainfall on dengue dynamics. By doing so, the model improves forecast skill over recent, state-of-the-art models for dengue incidence, providing empirical evidence that host population susceptibility and climate drive dengue dynamics in a nonlinear and complex, yet predictable way.
Review
Microbiology
Alexis Carpenter, Rollie J. Clem
Summary: Arboviral diseases transmitted by mosquitoes are a major global health concern. Current treatment and prevention methods are limited, so controlling the disease at the vector level is ideal. The midgut of the mosquito plays a crucial role in preventing the spread of arboviruses. Understanding the midgut escape barrier may aid in developing effective control strategies.
Article
Biochemistry & Molecular Biology
Adeline E. Williams, Gaurav Shrivastava, Apostolos G. Gittis, Sundar Ganesan, Ines Martin-Martin, Paola Carolina Valenzuela Leon, Ken E. Olson, Eric Calvo
Summary: The study investigates the RNA-binding dynamics and subcellular localization of AePiwi4, a Piwi protein in Aedes aegypti involved in antiviral immunity and embryonic development. It was found that the PAZ domain of AePiwi4 can bind to both mature and unmethylated RNAs in a sequence-independent manner. Furthermore, site-directed mutagenesis studies revealed the significant impact of subtle amino acid changes on RNA binding behaviors across PAZ proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Souheyla Benfrid, Kyu-Ho Park, Mariano Dellarole, James E. Voss, Carole Tamietti, Gerard Pehau-Arnaudet, Bertrand Raynal, Sebastien Brule, Patrick England, Xiaokang Zhang, Anastassia Mikhailova, Milena Hasan, Marie-Noelle Ungeheuer, Stephane Petres, Scott B. Biering, Eva Harris, Anavaj Sakuntabhai, Philippe Buchy, Veasna Duong, Philippe Dussart, Fasseli Coulibaly, Francois Bontems, Felix A. Rey, Marie Flamand
Summary: The NS1 protein of the dengue virus forms a high-affinity complex with human high-density lipoproteins (HDL), leading to inflammation and accumulation in plasma of dengue patients.
Article
Microbiology
Marcus Vinicius de Franca Cirilo, Shahab Zaki Pour, Viviane de Fatima Benedetti, Jessica Pires Farias, Mayanna Moreira Costa Fogaca, Rafael da Conceicao Simoes, Paloma Oliveira Vidal, Alexander Birbrair, Paolo Marinho de Andrade Zanotto, Wilson Barros Luiz, Jaime Henrique Amorim
Summary: This study conducted research in two main cities of Western Bahia, Brazil, and used molecular biology assays to detect various mosquito-borne viruses, including CHIKV, DENV, and ZIKV. The results showed the co-circulation of these viruses in the region, highlighting the importance of laboratory diagnosis in monitoring disease transmission.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Infectious Diseases
Barbara Batista Salgado, Fabio Carmona de Jesus Maues, Maele Jordao, Renato Lemos Pereira, Daniel A. Toledo-Teixeira, Pierina L. Parise, Fabiana Granja, Higo Fernando Santos Souza, Marcio Massao Yamamoto, Jannifer Oliveira Chiang, Livia Caricio Martins, Silvia Beatriz Boscardin, Jaila Dias Borges Lalwani, Pedro Fernando C. Vasconcelos, Jose Luiz Proenca-Modena, Pritesh Lalwani
Summary: This study investigated the serological response of residents in Manaus, Brazil, and found that 74.52% of participants had IgG positivity, 93.7% were seropositive for at least one DENV serotype, and there was a high degree of cross-reactivity with yellow fever and other flaviviruses. The study suggests that the population in the Manaus-Amazon region may be susceptible to emerging and endemic arboviruses.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Microbiology
Fernando Tobias Silveira, Edivaldo Costa Sousa, Rodrigo Vellasco Duarte Silvestre, Thiago Vasconcelos dos Santos, Wilfredo Sosa-Ochoa, Concepcion Zuniga Valeriano, Patricia Karla Santos Ramos, Samir Mansour Moraes Casseb, Luciana Vieira do Rego Lima, Marliane Batista Campos, Vania Lucia da Matta, Claudia Maria Gomes, Gabriela V. Araujo Flores, Carmen M. Sandoval M. Pacheco, Carlos Eduardo Corbett, Marcia Dalastra Laurenti
Summary: Visceral leishmaniasis (VL), also known as kala-azar, is caused by the Leishmania (L.) infantum chagasi parasite, which has been found to have a high genomic similarity (99%) with L. (L.) infantum. Understanding the characteristics of this disease and its transmission in the New World can aid in the development of targeted prevention and treatment strategies.
Article
Microbiology
Emilyn Costa Conceicao, Marilia Lima da Conceicao, Davi Josue Marcon, Johannes Loubser, Gabrielly Leite Andrade, Sandro Patroca da Silva, Ana Cecilia Ribeiro Cruz, Abhinav Sharma, Philip Suffys, Karla Valeria Batista Lima
Summary: This study characterized and analyzed the genetic diversity of Mycobacterium tuberculosis Central Asian Strain (CAS) Lineage 3 (L3) genotype in northern regions of Brazil. The results confirmed the presence of a novel genotype (CAS/SIT2545) belonging to L3. Further analysis showed genetic differences between the Brazilian strains and global strains. This highlights the importance of studying the genetic diversity of Mycobacterium tuberculosis in northern regions of Brazil.
Article
Parasitology
Fabio Silva da Silva, Bruna Lais Sena do Nascimento, Ana Cecilia Ribeiro Cruz, Sandro Patroca da Silva, Andressa de Oliveira Aragao, Daniel Damous Dias, Lucas Henrique da Silva e Silva, Lucia Aline Moura Reis, Jose Wilson Rosa, Durval Bertram Rodrigues Vieira, Daniele Barbosa de Almeida Medeiros, Joaquim Pinto Nunes Neto
Summary: This study reports the first complete sequencing of the mitochondrial genome of Limatus durhamii Theobald, 1901. The phylogeny reconstruction based on the concatenation of all 13 protein-coding genes further validates the taxonomic classification of this genus. These findings are important for the taxonomic and evolutionary studies of this genus and the Culicidae family as a whole.
Article
Virology
Milene S. S. Ferreira, Jorge R. R. Sousa, Pedro S. Bezerra Junior, Valiria D. Cerqueira, Carlos A. A. Oliveira Junior, Gabriela R. C. Rivero, Paulo H. G. Castro, Gilmara A. A. Silva, Jose Augusto P. C. Muniz, Eliana V. P. da Silva, Samir M. M. Casseb, Carla Pagliari, Livia C. Martins, Robert B. B. Tesh, Juarez A. S. Quaresma, Pedro F. C. Vasconcelos
Summary: This study investigates the virological, histopathological, and immunohistochemical findings in squirrel monkeys infected with yellow fever virus. The virus shows a preference for the middle zone of the liver and leads to jaundice, subcapsular hemorrhagic multifocal petechiae, apoptosis, necrosis, steatosis, and cellular edema. The immune response involves the expression of various markers, cytokines, and Treg cells.
Article
Virology
Ana Lucia Monteiro Wanzeller, Fabio Silva da Silva, Leonardo Henrique Almeida Hernandez, Landerson Junior Leopoldino Barros, Maria Nazare Oliveira Freitas, Maissa Maia Santos, Ercilia de Jesus Goncalves, Jamilla Augusta Sousa Pantoja, Creuza de Sousa Lima, Maxwell Furtado Lima, Luiz Roberto Oliveira Costa, Liliane Leal das Chagas, Iveraldo Ferreira Silva, Tania Cristina Alves da Silveira da Cunha, Bruna Lais Sena do Nascimento, Helena Baldez Vasconcelos, Elizabeth Salbe Travassos da Rosa, Sueli Guerreiro Rodrigues, Raimunda do Socorro da Silva Azevedo, Livia Caricio Martins, Livia Medeiros Neves Casseb, Jannifer Oliveira Chiang, Joaquim Pinto Nunes Neto, Ana Cecilia Ribeiro Cruz, Valeria Lima Carvalho, Pedro Fernando da Costa Vasconcelos, Eliana Vieira Pinto da Silva
Summary: Viruses such as Saint Louis encephalitis virus, Venezuelan equine encephalitis virus, Eastern equine encephalitis virus, Western equine encephalitis virus, Dengue virus, Zika virus, Chikungunya virus, Mayaro virus, and West Nile virus can cause neurological conditions of clinical and epidemiological importance. This study aimed to determine the number of arboviruses with neuroinvasive potential isolated in Brazil from 1954 to 2022. A total of 1,347 arbovirus samples were isolated from mice, 5,065 human samples were isolated exclusively by cell culture, and 676 viruses were isolated from mosquitoes. The detection of circulating arboviruses with neuroinvasive potential justifies the need for continued active epidemiological surveillance in Brazil.
Article
Microbiology
Yasmin Pacheco Ribeiro, Luiz Fabio Magno Falcao, Vanessa Cavaleiro Smith, Jorge Rodrigues de Sousa, Carla Pagliari, Edna Cristina Santos Franco, Ana Cecilia Ribeiro Cruz, Janniffer Oliveira Chiang, Livia Caricio Martins, Juliana Abreu Lima Nunes, Fellipe Souza da Silva Vilacoert, Lais Carneiro dos Santos, Matheus Perini Furlaneto, Hellen Thais Fuzii, Marcos Virgilio Bertonsin Filho, Luccas Delgado da Costa, Maria Irma Seixas Duarte, Ismari Perini Furlaneto, Arnaldo Jorge Martins Filho, Tinara Leila de Souza Aarao, Pedro Fernando da Costa Vasconcelos, Juarez Antonio Simoes Quaresma
Summary: In this study, histopathological analysis of liver samples from 70 patients who died due to infections and complications of yellow fever, dengue fever, and chikungunya fever was conducted. The results showed significant differences in histopathological alterations between the control and infection groups, with a predominance of changes in the midzonal area in all three cases analyzed. Hepatic involvement in yellow fever cases showed more severe histopathological changes.
Article
Infectious Diseases
Ana Cecilia Ribeiro Cruz, Leonardo Henrique Almeida Hernandez, Carine Fortes Aragao, Thito Yan Bezerra da Paz, Sandro Patroca da Silva, Fabio Silva da Silva, Ana Alice de Aquino, Glennda Juscely Galvao Pereira Cereja, Bruna Lais Sena do Nascimento, Jose Wilson Rosa Junior, Carmeci Natalina Elias, Cristiano Gomes Nogueira, Daniel Garkauskas Ramos, Vagner Fonseca, Marta Giovanetti, Luiz Carlos Junior Alcantara, Bruno Tardelli Diniz Nunes, Pedro F. da Costa Vasconcelos, Livia Caricio Martins, Joaquim Pinto Nunes-Neto
Summary: The largest outbreak of sylvatic yellow fever virus (YFV) in Brazil between 2016-2018 was reported, and entomo-virological surveillance was considered important for monitoring YFV. Mosquitoes were collected from six Brazilian states and tested for YFV using RT-qPCR. YFV infection was detected in various mosquito species, including Ae. albopictus, indicating the potential for urban YFV re-emergence. The study highlights the need to strengthen YFV surveillance, vaccination coverage, and vector control measures in Brazil.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Murilo Tavares Amorim, Leonardo H. Almeida Hernandez, Felipe Gomes Naveca, Ivy Tsuya Essashika Prazeres, Ana Lucia Monteiro Wanzeller, Eliana Vieira Pinto da Silva, Livia M. Neves Casseb, Fabio Silva da Silva, Sandro Patroca da Silva, Bruno Tardelli Diniz Nunes, Ana Cecilia Ribeiro Cruz
Summary: In this study, 163 human serum samples from the DENV outbreak in Acre, Northern Brazil between 2020 and 2021 were tested for DENV genotypes by RT-qPCR. Of these samples, 139 were positive for DENV-2, and 5 were positive for DENV-1. Sequencing of 5 DENV-2-positive samples from early 2021 revealed clustering with previously recorded DENV-2 cosmopolitan genotype sequences in South America. These findings suggest a possible route of introduction of the DENV-2 cosmopolitan genotype into Brazil from Peru, followed by dispersal to the Midwest region.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Genetics & Heredity
Marcos Jesse Abrahao Silva, Caroliny Soares Silva, Rebecca Lobato Marinho, Jeanne Goncalves Cabral, Ellen Polyana da Costa Gurrao, Pabllo Antonny Silva dos Santos, Samir Mansour Moraes Casseb, Karla Valeria Batista Lima, Luana Nepomuceno Gondim Costa Lima
Summary: The objective of this study was to verify the associations between epidemiological factors and SNP in the TLR2 gene, and to identify risk factors and their impact on the stability of the TLR2 protein in COVID-19. Associations were found between comorbidities and poor prognosis, as well as family members' health and relatedness to the disease.
Article
Genetics & Heredity
Carine Fortes Aragao, Sandro Patroca da Silva, Bruna Lais Sena do Nascimento, Fabio Silva da Silva, Joaquim Pinto Nunes Neto, Valeria Cristina Soares Pinheiro, Ana Cecilia Ribeiro Cruz
Summary: This study investigated the viral diversity in mosquitoes from a transition area in Brazil and found a large number of previously unknown viruses, but no viruses associated with human and animal diseases were detected.
Article
Virology
Tiago Graf, Caroline Do Nascimento Ferreira, Gustavo Barbosa de Lima, Raul Emidio de Lima, Lais Ceschini Machado, Tulio de Lima Campos, Michelle Orane Schemberger, Helisson Faoro, Marcelo Henrique Santos Paiva, Matheus Filgueira Bezerra, Valdinete Nascimento, Victor Souza, Fernanda Nascimento, Matilde Mejia, Dejanane Silva, Yasmin Silva de Oliveira, Luciana Goncalves, Tatyana Costa Amorim Ramos, Daniel Barros de Castro, Ana Ruth Arcanjo, Herton Augusto Pinheiro Dantas, Mayra Marinho Presibella, Sandra Bianchini Fernandes, Tatiana Schaffer Gregianini, Keilla Maria Paz E Silva, Claudio Tavares Sacchi, Ana Cecilia Ribeiro Cruz, Claudia Nunes Duarte dos Santos, Ana Maria Bispo de Filippis, Gonzalo Bello, Gabriel Luz Wallau, Richard Steiner Salvato, Felipe Naveca
Summary: Dengue virus serotype 2, genotype Cosmopolitan (DENV-2-GII), is circulating widely in Brazil and has been introduced multiple times, leading to localized outbreaks. However, previous circulation of genotype Asian-American (DENV-2-GIII) may have created an immune barrier against DENV-2-GII in certain regions of Brazil.
Article
Veterinary Sciences
Tania Cristina Alves da Silveira da Cunha, Fabio Silva da Silva, Sandro Patroca da Silva, Ana Cecilia Ribeiro Cruz, Francisco Amilton dos Santos Paiva, Livia Medeiros Neves Casseb, Ana de Nazare Silva do do Nascimento, Iza Alencar Sampaio de Oliveira, Marlon de Araujo Castelo Branco, Rodrigo Adolpho Brasil de Oliveira, Darlene de Brito Simith Durans, Thito Yan Bezerra da Paz, Taciana Fernandes Souza Barbosa Coelho
Summary: This study identified six antigenic variants in rabies surveillance in northern and northeastern Brazil through phylogenetic analysis. The discovery of the AgV6 variant in northern Brazil for the first time provides valuable information for rabies surveillance in the country. Further genomic surveillance studies are needed to understand the transmission dynamics and geographic distribution changes of each variant.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Chemistry, Medicinal
Francisco Caninde Ferreira de Luna, Wallax Augusto Silva Ferreira, Samir Mansour Moraes Casseb, Edivaldo Herculano Correa de Oliveira
Summary: Flavonoids, natural compounds with pharmacological activity, have gained increasing attention in scientific research focused on therapeutic purposes. Tangeretin, a flavonoid found in citrus fruits, exhibits various anti-cancer activities in different types of cancerous cell lines, making it a promising candidate for optimizing cancer treatments and developing new anti-cancer drugs.
Article
Biochemistry & Molecular Biology
Samir Mansour Moraes Casseb, Karla Fabiane Lopes de Melo, Carlos Alberto Marques de Carvalho, Carolina Ramos dos Santos, Edna Cristina Santos Franco, Pedro Fernando da Costa Vasconcelos
Summary: The World Health Organization estimates that there are approximately 392 million cases of dengue virus (DENV) infections annually in more than 100 endemic countries, posing a serious threat to humanity. Dengue is the most widespread mosquito-borne disease in the world. This study investigates the relationship between NS1 protein, miRNAs-15/16, and apoptosis during DENV-4 infection in human liver cell lines.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2023)
Article
Medicine, General & Internal
Adriano da Paixao Souto, Samir Mansour Moraes Casseb, Karla Fabiane Lopes de Melo, Arnaldo Jorge Martins Filho, Edna Cristina Santos Franco
Summary: This study demonstrated the efficient replication of the Rocio virus in primary cultured neural cells from mice. The infection was confirmed through immunocytochemistry and viral replication was quantified. The results suggest that mouse neural cell primary cultures could be an alternative system for studying Flavivirus infection in the central nervous system.
EINSTEIN-SAO PAULO
(2023)
