Article
Forestry
Gessica Ferreira da Costa, Pablo Diego Silva Cabral, Fabiano Guimaraes Silva, Aurelio Rubio Neto, Maria Andreia Correa Mendonca
Summary: This study evaluated the genetic stability of H. speciosa plantlets and calluses using molecular markers, and found that the tissue culture techniques for H. speciosa micropropagation did not influence somaclonal variation, and the donor plants showed relatively low genetic variability.
Article
Agricultural Engineering
Kuldeep Kaur, Pritika Singh, Kulwinder Kaur, Abhishek Bhandawat, Panchsheela Nogia, Pratap Kumar Pati
Summary: Withania somnifera is a medicinal plant with immense pharmaceutical potential. In vitro propagation system is used to produce genetically and phytochemically uniform plants, with a multi-step protocol developed to minimize the adverse effects of cytokinin. The protocol also includes modulation of ammonium nitrate strength for in vitro rooting initiation.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Abhijit Dey, Potsangbam Nongdam, Samapika Nandy, Sabyasachi Mukherjee, Anuradha Mukherjee, Leimapokpam Tikendra, Alok Kumar Hazra, Devendra Kumar Pandey
Summary: The study investigated the effects of different polyamines and plant growth regulators on multiplication, rooting, callusing, and regeneration in Aristolochia indica L. in vitro. The results showed that the growth and regeneration process of A. indica plants in SH media were significantly influenced by polyamines and plant growth regulators, especially in terms of callusing and rooting.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Agronomy
Argelys Kessel-Domini, Daisy Perez-Brito, Adolfo Guzman-Antonio, Felipe A. Barredo-Pool, Javier O. Mijangos-Cortes, Lourdes Georgina Iglesias-Andreu, Alberto Cortes-Velazquez, Adriana Canto-Flick, Susana A. Aviles-Vinas, Yaritza Rodriguez-Llanes, Nancy Santana-Buzzy
Summary: The objective of this study was to establish an efficient regeneration system for pineapple plants, with a high multiplication rate while preserving the genetic identity of the donor genotype. Different hormone treatments were used to induce morphogenesis, and various regeneration routes were identified. It was demonstrated that the regenerated pineapple plants in vitro maintained the genetic characteristics of the donor genotype, validating the use of indirect somatic embryogenesis for clonal propagation of A. comusus.
Article
Plant Sciences
Adel Zarei, Biruk A. A. Feyissa, Benjamin Davis, Elham Tavakouli Dinani
Summary: This study developed a reliable protocol for cannabis synthetic seed production using encapsulation of nodal segments derived from in vitro or in vivo sources. Synthetic seeds stored for up to 150 days showed regrowth rates of 70% and 90% from in vitro and in vivo sources, respectively, and addition of acetylsalicylic acid (ASA) improved regrowth rate to 100%. Exposure to light during storage significantly increased shoot length of regrown synseeds, and all regenerated plantlets were rooted and acclimatized without morphological changes.
Article
Horticulture
Naseem Ahmad, Mohammad Faisal, Anees Ahmad, Abdulrahman A. Alatar, Ahmed A. Qahtan, Anshu Alok
Summary: A high-yield stable virus expression system has been developed, which is suitable for large-scale production of target proteins and preparation of antibodies.
Article
Plant Sciences
Mohammed Alsafran, Kokila Wickramanayake, Kamal Usman, Talaat Ahmed
Summary: In vitro plant cell and tissue cultures are powerful tools in conserving and managing plant genetic resources. A reliable micropropagation protocol was developed for efficient callus proliferation and shoot regeneration of Meseika plants. This protocol allows for rapid in-vitro multiplication of Meseika shoots and callus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
C. P. Krishna Vrundha, T. Dennis Thomas
Summary: An efficient shoot regeneration protocol was developed for Thottea siliquosa via indirect shoot regeneration from root explants. The best callus induction and shoot induction responses were obtained with specific combinations of plant growth regulators. The field established in vitro propagated plants showed higher phytochemical content and antioxidant activity compared to the mother plants and root derived callus.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Gargi Prasad, Tapan Seal, Ashiho A. Mao, Deepu Vijayan, Adani Lokho
Summary: A genetically stable in vitro regeneration protocol was established for mass multiplication of Bulbophyllum odoratissimum using a synergistic effect of BA and IBA. IBA was found to be more effective for root proliferation. The well-acclimatized plants exhibited normal development and both mother plants and in vitro raised plants contained relevant phytochemical compounds.
SOUTH AFRICAN JOURNAL OF BOTANY
(2021)
Article
Chemistry, Medicinal
Leimapokpam Tikendra, Oinam Sushma, Thoungamba Amom, NandeibamApana Devi, Sonia Paonam, Nongthombam Bidyananda, AngambaMeetei Potshangbam, Abhijit Dey, RajkumariSanayaima Devi, Potshangbam Nongdam
Summary: In this study, a reliable in vitro regeneration protocol was developed for the rapid propagation of Acorus calamus plants, and the clonal fidelity of the regenerants was assessed using molecular markers. The results indicated that ISSR markers were more suitable for clonal fidelity assessment compared to RAPD markers.
PHARMACOGNOSY MAGAZINE
(2022)
Article
Biotechnology & Applied Microbiology
Fangmei Zhou, Weipeng Huang, Wen Cheng, Jingjing Lu, Bingqi Zhu, Jie Yu, Zhishan Ding, Qianxing Jin
Summary: This study optimized the medium for induction and differentiation of PLBs from Pleione bulbocodioides, maintaining the DNA hereditary stability and ultrastructural integrity. The optimal medium formulations were identified for inducing protocorms and PLBs, leading to no DNA polymorphisms during the process. This finding is beneficial for efficient micropropagation of Pleione bulbocodioides.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Kostas Ioannidis, Ioanna Tomprou, Vangelis Mitsis, Polyxeni Koropouli
Summary: SSR markers were used to evaluate the genetic stability of micropropagated and regenerated plants in Cannabis sativa, showing the reliability of in vitro propagation and its potential for clonal mass propagation.
Article
Plant Sciences
Thirupathi Koppula, Dulam Sandhya, Gulab Khan Rohela, Saritha Kommidi, Mustafa Mohammed
Summary: Solena amplexicaulis is a creeping cucurbit with high medicinal values. In this study, in vitro micropropagation was successfully used as an alternative method for its propagation and conservation. By optimizing the plant growth regulators and culture conditions, a large number of shoots were induced and subsequently rooted. The resulting plantlets showed high survival rates when transferred to natural field conditions. The genetic fidelity of tissue-cultured plants was also confirmed.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2023)
Article
Environmental Sciences
N. S. Bhandari, C. R. Aswath, D. C. L. Reddy, C. Nagaraju
Summary: This study aimed to develop a protocol for mass multiplication of Lilium (Lilium longiflorum) bulbs through indirect organogenesis and somatic embryogenesis. The best callus mediated plant regeneration method was found to be BAP (0.5 mg l(-1)) + 2,4-D (3.0 mg l(-1)) combination, while the best medium for embryogenic callus initiation and proliferation was MS medium containing 0.50 mg l(-1) picloram and 0.20 mg l(-1) NAA. The clonal fidelity of the regenerated plantlets was confirmed using ISSR markers. The findings of this study have potential applications in mass multiplication and genetic transformation studies of Lilium species.
JOURNAL OF ENVIRONMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Wei Quan Pang, Chern Shun Lai, Mohamad Fadhli Mad Atari, Bothi Raja Pandian, Mohamad Nasir Mohamad Ibrahim, Swee Tiam Tan, Ivan Chew Ken Yoong, Sreeramanan Subramaniam
Summary: Graphene oxide (GO) is a novel nanomaterial with distinct physical properties and significant biological applications. This study focused on the effects of GO on the regeneration process and genetic stability of white strawberry plants, finding that lower concentrations of GO can improve plant development and stability, making it suitable for strawberry tissue culture.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Green & Sustainable Science & Technology
Stefanos Kostas, Aikaterini Kaplani, Efthymia Koulaouzidou, Aikaterini-Angeliki Kotoula, Evangelos Gklavakis, Parthena Tsoulpha, Stefanos Hatzilazarou, Irini Nianiou-Obeidat, Angelos K. Kanellis, Athanasios Economou
Summary: This study investigated the morphological traits of seven wild-grown rosemary populations in Greece and focused on the propagation of two selected populations with desirable ornamental characteristics. Shoot cuttings and in vitro cultures were used for propagation, and different conditions and mediums were tested for rooting and shoot formation. The research successfully achieved rosemary plantlets with roots formed in vitro and acclimatized them in a greenhouse environment.
Article
Biology
Stefanos Kostas, Stefanos Hatzilazarou, Elias Pipinis, Soumaya Bourgou, Imtinen Ben Haj Jilani, Wafa Ben Othman, Wided Megdiche-Ksouri, Zeineb Ghrabi-Gammar, Mohamed Libiad, Abdelmajid Khabbach, Mohamed El Haissoufi, Fatima Lamchouri, Emmanouil Koundourakis, Vasileios Greveniotis, Evgenia Papaioannou, Michalia A. Sakellariou, Ioannis Anestis, Georgios Tsoktouridis, Nikos Krigas
Summary: This study focused on the Tunisian local endemic Teucrium luteum subsp. gabesianum, using Geographical Information Systems and online databases to create a detailed ecological profile for the taxon. The study also investigated the effect of temperature on seed germination and explored the growth response of seedlings to different types of fertilizers. The use of DNA barcoding provided insights into the taxonomic circumscription of Teucrium taxa, enabling future traceability and product design. Overall, this multidisciplinary approach bridged research gaps and highlighted the potential value of the plant for medicinal, cosmetic, agro-alimentary, and ornamental purposes.
Article
Green & Sustainable Science & Technology
Styliani Alexandri, Maria Tsaktsira, Stefanos Hatzilazarou, Stefanos Kostas, Irini Nianiou-Obeidat, Athanasios Economou, Apostolos Scaltsoyiannes, Parthena Tsoulpha
Summary: Pyrus spinosa Forssk. (almond-leaved pear) is a wild-growing native tree in Greece that has attracted attention for its valuable endogenous substances. This study determined the total phenol content (TPC) and antioxidant capacity (AC) of leaves from 32 genotypes of mature wild-growing trees at different locations in Central Macedonia District, Greece. The results showed a strong positive correlation between TPC and AC, and two genotypes from Agia Anastasia exhibited the highest TPC and AC. An efficient micropropagation protocol was developed for these genotypes to preserve and multiply the valuable germplasm.
Article
Plant Sciences
Elias Pipinis, Stefanos Hatzilazarou, Stefanos Kostas, Rafaela Stagiopoulou, Konstantina Gitsa, Eleftherios Dariotis, Ioulietta Samartza, Ioannis Plastiras, Eleni Kriemadi, Pepy Bareka, Christos Lykas, Georgios Tsoktouridis, Nikos Krigas
Summary: In order to understand the effect of temperature on seed germination, we evaluated the germination responses of two endangered local endemic tulip species of Greece (Tulipa hageri Heldr., T. orphanidea Heldr.) at five constant temperatures (5, 10, 15, 20, and 25 degrees C). The results indicated a range-specific temperature dependence in seed germination for both species, with germination observed only at very low temperatures (5-10 degrees C). The presence of complex morphophysiological seed dormancy was confirmed in both species.
Article
Plant Sciences
Stefanos Hatzilazarou, Elias Pipinis, Stefanos Kostas, Rafaela Stagiopoulou, Konstantina Gitsa, Eleftherios Dariotis, Manolis Avramakis, Ioulietta Samartza, Ioannis Plastiras, Eleni Kriemadi, Pepy Bareka, Christos Lykas, Georgios Tsoktouridis, Nikos Krigas
Summary: This study investigated the effect of temperature on seed germination of Greek tulip species. The results showed that the seed germination of the studied species had a range-specific temperature dependence. The seeds germinated only at low temperatures (5-15 degrees C).
Article
Horticulture
Stefanos Hatzilazarou, Stefanos Kostas, Elias Pipinis, Ioannis Anestis, Evgenia Papaioannou, Vasiliki Aslanidou, Parthena Tsoulpha, Manolis Avramakis, Nikos Krigas, George Tsoktouridis
Summary: This study focuses on the conservation and sustainable utilization of two local endemic plants on Crete (Greece), Lomelosia minoana and Eryngium ternatum. By examining the ecological profiles and the effects of temperature and fertilization on their growth and nutrient content, this study provides insights into their optimal growing conditions and recommendations for their sustainable exploitation.
Article
Horticulture
Christos Nanos, Parthena Tsoulpha, Stefanos Kostas, Stefanos Hatzilazarou, Ioanna Michail, Vasiliki Anastasiadi, Elias Pipinis, Evangelos Gklavakis, Angelos K. Kanellis, Irini Nianiou-Obeidat
Summary: In this study, 12 native sage populations in north-west Greece were evaluated for their ornamental traits, and populations from Aristi, Kefalovryso, and Igoumenitsa were selected for their best performance and investigated for their asexual propagation through shoot cutting and in vitro techniques. The results showed that Aristi had the highest rooting performance in spring by applying 0.5 g/L IBA and had the highest number of newly formed shoots and shoot length in the in vitro propagation. Aristi also showed potential as a competitive ornamental variety.
Article
Agronomy
Elias Pipinis, Athanasios Stampoulidis, Aikaterini Angeliki Kotoula, Elias Milios, Stefanos Kostas, Stefanos Hatzilazarou, Evgenia Papaioannou, Alexandros Papaeirinaios, Kyriaki Kitikidou, Kalliopi Radoglou
Summary: Arbutus andrachne, a valuable phytogenetic resource, is an evergreen shrub or small tree native to the Mediterranean basin. This study provided a detailed germination protocol, molecular analysis, and cultivation practices for four Greek natural populations of A. andrachne. Cold stratification or gibberellic acid treatment resulted in similar germination patterns for all populations. Morphological traits showed differences among populations, and plastic bag cultivation showed superior growth rates. Fertilizer supplementation enhanced foliage mass for 2-year-old seedlings. Genetic variability was found within populations rather than among them.
Article
Biology
Stefanos Hatzilazarou, Ioannis Anestis, Elias Pipinis, Stefanos Kostas, Manolis Avramakis, Vasileios Greveniotis, Eleftherios Dariotis, Georgios Tsoktrouridis, Nikos Krigas
Summary: This study aimed to determine the ecological conditions for the growth of six Cretan endemic plants and their potential for sustainable exploitation in various economic sectors. The germination requirements of these plants were analyzed and species-specific propagation protocols were developed.
JOURNAL OF BIOLOGICAL RESEARCH-THESSALONIKI
(2023)
Article
Ecology
Elias Pipinis, Stefanos Kostas, Stefanos Hatzilazarou, Sofoklis Petropoulos, Dimitra Mitsi, Athanasios Stampoulidis, Elias Milios, Pavlos Smiris
Summary: The study investigated the effects of donor tree age, collection time, and K-IBA application on rooting of T. baccata stem cuttings. The results showed that the application of K-IBA significantly improved the rooting percentage in adult individuals, while in juvenile individuals, the collection time and K-IBA application had a significant effect. In November collection, the highest rooting percentage was observed in cuttings treated with 12 g L-1 K-IBA, while in March collection, cuttings treated with 3 g L-1 K-IBA showed higher rooting percentages compared to the control.