Intrinsic activation of β-catenin signaling by CRISPR/Cas9-mediated exon skipping contributes to immune evasion in hepatocellular carcinoma

Comprehensive analysis of clinical samples has recently identified molecular and immunological classification of hepatocellular carcinoma (HCC), and the CTNNB1 (beta-catenin)-mutated subtype exhibits distinctive characteristics of immunosuppressive tumor microenvironment. For clarifying the molecular mechanisms, we first established human and mouse HCC cells with exon 3 skipping of beta-catenin, which promoted nuclear translocation and activated the Wnt/beta-catenin signaling pathway, by using newly developed multiplex CRISPR/Cas9-based genome engineering system. Gene set enrichment analysis indicated downregulation of immune-associated gene sets in the HCC cells with activated beta-catenin signaling. Comparative analysis of gene expression profiles between HCC cells harboring wild-type and exon 3 skipping beta-catenin elucidated that the expression levels of four cytokines were commonly decreased in human and mouse beta-catenin-mutated HCC cells. Public exome and transcriptome data of 373 human HCC samples showed significant downregulation of two candidate cytokine genes, CCL20 and CXCL2, in HCC tumors with beta-catenin hotspot mutations. T cell killing assays and immunohistochemical analysis of grafted tumor tissues demonstrated that the mouse Ctnnb1(Delta ex3) HCC cells evaded immunosurveillance. Taken together, this study discovered that cytokine controlled by beta-catenin signaling activation could contribute to immune evasion, and provided novel insights into cancer immunotherapy for the beta-catenin-mutated HCC subtype.

Automated summary

Comprehensive analysis of clinical samples has identified molecular and immunological classification of hepatocellular carcinoma, with the CTNNB1-mutated subtype showing characteristics of immunosuppressive tumor microenvironment. Activation of beta-catenin signaling in HCC cells contributes to immune evasion through downregulation of cytokines, providing insights into cancer immunotherapy for this subtype.