Article
Biochemistry & Molecular Biology
Filippo Mori, Letizia Natali, Romano Danesi, Silvia Nannizzi, Claudio Farina
Summary: The study examined seven commercially available HSA preparations for clinical use and found differences in PTMs among different commercial albumins. Additionally, an increase in HSA-DA isoform and new oxidative modifications were observed in these samples at the end of the stability time.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Neurosciences
Faris Almasabi, Faisal Alosaimi, Minerva Corrales-Terron, Anouk Wolters, Dario Strikwerda, Jasper Smit, Yasin Temel, Marcus L. F. Janssen, Ali Jahanshahi
Summary: This study conducted post-mortem histopathological assessment to reveal structural changes in auditory and non-auditory regions of tinnitus patients. The findings suggest that neurodegenerative and inflammatory processes may underlie the neuropathology of tinnitus, and the raphe serotonergic system may play a role in tinnitus.
Article
Biochemistry & Molecular Biology
Jimin Park, Mi-Sun Kim, Taeseong Park, Young Hwan Kim, Dong Hae Shin
Summary: Pharmaceutical-grade HSA has altered oxidative and ligand-binding properties compared to native HSA, which provides important insights for the production of p-HSA solutions and HSA-based biomaterials with higher biorelevance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Toxicology
Yoshikazu Yamagishi, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra
Summary: DMTP, a widely used organophosphorus pesticide, has been detected in clinical cases of accidental exposure and suicide. This study found that DMTP forms adducts with specific residues of human serum albumin, which can serve as biomarkers for DMTP poisoning.
JOURNAL OF TOXICOLOGICAL SCIENCES
(2022)
Article
Ecology
Nengi Ogbanga, Andrew Nelson, Sarah Gino, Daniel J. Wescott, Hayley L. Mickleburgh, Timothy P. Gocha, Noemi Procopio
Summary: Human donations are used in forensic research to provide unique insights into post-mortem research. Freezing does not significantly alter the human microbiome, thus frozen donors are suitable for forensic studies.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Biochemistry & Molecular Biology
Atala B. Jena, Rashmi R. Samal, Jagneshwar Dandapat, Umakanta Subudhi
Summary: Biological macromolecules, such as proteins, nucleic acids, carbohydrates and lipids, are essential for biochemical and molecular processes. This study investigates the interaction between the benzene metabolite para-benzoquinone (BQ) and nucleic acids (B-DNA) and human serum albumin (HSA). The results show that BQ affects the binding ability and structure of HSA, potentially impacting plasma osmotic pressure and the binding of molecules. BQ also interacts with the GC region of B-DNA, potentially inducing mutagenicity and oxidative damage.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Nanoscience & Nanotechnology
Qiongwei Wu, Qianqian Hou, Peipei Wang, Caifeng Ding, Shaoping Lv
Summary: This study reports a novel redox-responsive sol-gel transition spinning method for the continuous spinning of polybenzimidazole/cobalt ion (Co) aerogel fibers. The coordination property changes of imidazole ligands and Co with different redox states are utilized to construct a robust crosslinked network in the spinning process. The obtained aerogel fibers demonstrate homogeneous microporous structure, high mechanical strength, low thermal conductivity, excellent thermal stability, and flame resistance, showing promise for protection applications under extreme environments.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Medicinal
Yoshikazu Yamagishi, Hirotaro Iwase, Yasumitsu Ogra
Summary: The study found that methomyl may be decomposed by human serum albumin or bound to specific blood proteins, resulting in lower methomyl concentrations in blood than expected. However, methomyl specifically binds to certain residues in hemoglobin.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Chemistry, Analytical
Deepak Kumar, Rajasri Bhattacharyya, Dibyajyoti Banerjee
Summary: In this study, Fast Blue BB was identified to specifically stain the pseudoesterase activity of HSA in gel, with 2-NA as the ester substrate. The addition of neostigmine did not inhibit this activity, providing specificity to the method. A simple and novel method for HSA detection on gel was devised, allowing for accurate detection without interference from other esterases.
Article
Medicine, Legal
Fabio De-Giorgio, Gabriele Ciasca, Gennaro Fecondo, Alberto Mazzini, Riccardo Di Santo, Marco De Spirito, Vincenzo L. Pascali
Summary: In this study, CT imaging was used to evaluate anatomical changes in the brain of corpses within 0-4 days after death. Fractal analysis of CT brain slices provided quantitative descriptors that can map post-mortem changes over time in the entire brain. While these descriptors cannot directly estimate the post-mortem interval, they can be combined with other methods to improve the accuracy and reliability of estimating the time since death.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Arianna De Rosa, Anna Di Maio, Silvia Torretta, Martina Garofalo, Valentina Giorgelli, Rita Masellis, Tommaso Nuzzo, Francesco Errico, Alessandro Bertolino, Srinivasa Subramaniam, Antonio Rampino, Alessandro Usiello
Summary: This study investigates the role of RasGRP1 in schizophrenia (SCZ) and its potential as a biomarker and therapeutic target. The results suggest that RasGRP1 may mediate the risk for SCZ by involving the dorsolateral-prefrontal cortex (DLPFC) and peripheral blood. Further studies are encouraged to explore its mechanisms and clinical applications.
Review
Biochemistry & Molecular Biology
Surya Kannan, Serhiy Souchelnytskyi
Summary: This study reviews novel post-translational modifications (PTMs) of human serum albumin, including phosphorylation, glycation, methylation, carbonylation, and acetylation. The potential impact of these modifications on albumin functions is discussed, highlighting their importance in medical applications.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Akankcha Gupta, Manal Khursheed, Zarina Arif, Asim Badar, Khursheed Alam
Summary: In healthy individuals, a small fraction of methylglyoxal reacts with macromolecules to form AGEs. However, in diabetics, the formation of methylglyoxal is increased, leading to its retention in cells and plasma. The resulting MG-HSA AGEs can cause damage to human embryonic kidney cells and trigger powerful inflammatory responses, potentially contributing to nephrotoxicity in diabetics.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Agriculture, Dairy & Animal Science
Charles-Antoine Assenmacher, Matthew Lanza, James C. Tarrant, Kristin L. Gardiner, Eric Blankemeyer, Enrico Radaelli
Summary: Routine manipulation and handling of laboratory mice can cause physical harm and confounding effects on research outcomes. Our study provides a comprehensive overview of the main pathological changes associated with these procedures, with an emphasis on traumatic osteoarticular lesions. Training research personnel on appropriate mouse handling techniques could help reduce the frequency of these lesions and their impact on animal wellbeing and study reproducibility.
Article
Biochemistry & Molecular Biology
Alexey Chubarov, Anna Spitsyna, Olesya Krumkacheva, Dmitry Mitin, Daniil Suvorov, Victor Tormyshev, Matvey Fedin, Michael K. Bowman, Elena Bagryanskaya
Summary: The Pulsed Dipolar Spectroscopy (PDS) methods of Electron Paramagnetic Resonance (EPR) were used to study reversible non-covalent dimers of Human Serum Albumin (HSA), showing that chemical modifications of Cys-34 can affect dimerization. The weak non-covalent dimers formed by HSA at physiological concentrations have a well-defined structure and can easily revert back to monomers, playing an important role in transport and binding processes.
Article
Toxicology
Sayaka Nagasawa, Rutsuko Yamaguchi, Kanju Saka, Suguru Torimitsu, Fumiko Chiba, Daisuke Yajima, Go Inokuchi, Ayumi Motomura, Kei Kira, Yoshikazu Yamagishi, Yasumitsu Ogra, Hirotaro Iwase
Summary: Ropinirole is an effective antiparkinsonian drug that has been suggested to be used in amyotrophic lateral sclerosis. This study reports a fatal case of ropinirole intoxication and compares the concentration levels with other non-poisoning cases. The results indicate a significantly higher concentration level in the fatal case, suggesting death due to shock and fatal arrhythmia as a result of ropinirole poisoning.
FORENSIC TOXICOLOGY
(2022)
Article
Chemistry, Analytical
Yu-ki Tanaka, Shunsuke Shimazaki, Yasunori Fukumoto, Yasumitsu Ogra
Summary: A rapid and precise quantification method for a histidine-tagged recombinant protein produced in Escherichia coli (E. coli) has been developed using single-cell inductively coupled plasma-mass spectrometry (SC-ICP-MS). This method allows for the determination of protein quantity based on the signal of nickel or cobalt ions bound to the His-tag, without the need for extraction and purification processes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Medicinal
Yoshikazu Yamagishi, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra
Summary: Oxime-type carbamate pesticides can be decomposed by Hb in post-mortem cases, leading to changes in their concentration and affecting the accurate determination of cause of death.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Article
Environmental Sciences
Yoshinari Suzuki, Midori Kondo, Hiroshi Akiyama, Yasumitsu Ogra
Summary: This study investigated the formation of nano-sized particles of mercury (NP-Hg) in fish and shellfish, which are the main sources of human exposure to mercury. The results showed that NP-Hg was present in almost all fish samples, with higher concentrations observed in tuna and swordfish. The study also estimated the dietary exposure to NP-Hg in Japanese people and found it to be relatively low, indicating a small health risk from redissolved inorganic mercury.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Yasunori Fukumoto, Masayoshi Ikeuchi, Yuji Nakayama, Yasumitsu Ogra
Summary: This study reveals that Rad17 protein has nucleolar localization signal and its accumulation in nucleolus is promoted by UV irradiation, suggesting a possible role of Rad17 in nucleolus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yoshikazu Yamagishi, Go Inokuchi, Yumi Hoshioka, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra
Summary: Paliperidone, a widely used antipsychotic agent, was found to be decomposed by hemoglobin through the Fenton reaction, resulting in the formation of the metabolite PM1. The detection of PM1 in the blood can serve as a biomarker to correct for the concentration of paliperidone at the time of death in forensic cases.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2023)
Article
Chemistry, Organic
Yasumasa Hara, Teruhisa Manome, Wataru Suehiro, Shinji Harada, Yoshikazu Yamagishi, Akiko Takaya, Yasumitsu Ogra, Masami Ishibashi
Summary: Two new octadeca-peptibols, trichorzin PAX and XI (1 and 2), were isolated from the fungus Trichoderma harzianum IFM 66736, obtained from the soil in Aobanomori Park, Chiba City, Japan. The structures of the compounds were determined through spectral studies, X-ray crystallographic analysis, and MS/MS analysis. Compound 1 and 2 exhibited cytotoxicity against human colon cancer cell lines HCT116.
TETRAHEDRON LETTERS
(2023)
Article
Food Science & Technology
Kazuaki Takahashi, Sakie Horiai, Yoshikazu Yamagishi, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra
Summary: This study reveals the biotransformation of a common vegetable selenium compound by gut microbe Morganella morganii, which provides efficient metabolic support for animals.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Chemistry, Analytical
Ryu Mochizuki, Akane Kobayashi, Hiromitsu Takayama, Toshihiko Toida, Yasumitsu Ogra
Summary: The Konig reaction is a useful method for detecting cyanide and its derivatives. In this study, we found that it can also be used to quantify glutathione and applied it to determine the levels of reduced and oxidized glutathiones. The method showed good sensitivity and was successfully applied to measure GSH and GSSG levels in cells exposed to oxidative stress.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Analytical
Yoshinari Suzuki, Midori Kondo, Masae Harimoto, Yusuke Okamoto, Yu-ki Tanaka, Yasumitsu Ogra, Hiroshi Akiyama
Summary: Single-particle ICP-MS is a method for determining the mass distribution and particle number concentration of inorganic nanoparticles. Researchers developed a Bayesian estimation method to analyze the data obtained from spICP-MS analysis of silver and silica nanoparticles. The Bayesian method provided better estimation results, especially for samples with high particle number concentration, compared to conventional criteria-based methods.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Article
Physics, Fluids & Plasmas
Yu-ki Tanaka, Taiki Osawa, Yoshikazu Yamagishi, Akitoshi Okino, Yasumitsu Ogra
Summary: This study demonstrates that cold atmospheric pressure plasma jet irradiation can effectively degrade methicillin and vancomycin, providing a powerful and environmentally friendly method for antibiotic treatment.
Article
Biochemistry & Molecular Biology
Shohei Takada, Yu-ki Tanaka, Kazuhiro Kumagai, Keita Kobayashi, Akiko Hokura, Yasumitsu Ogra
Summary: This study investigates the mechanism of tellurium (Te) nanorod formation in Chlamydomonas reinhardtii. It is found that the alga initially metabolizes tellurate to form soluble Te compounds, and excess tellurate is then transformed into low-toxicity Te nanorods.
Review
Nutrition & Dietetics
Yasumitsu Ogra, Yu-ki Tanaka, Noriyuki Suzuki
Summary: This review highlights recent advances in analytical techniques such as speciation, imaging, and single-cell analysis to understand copper metabolism.
JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION
(2022)
Article
Toxicology
Yoshikazu Yamagishi, Sayaka Nagasawa, Hirotaro Iwase, Yasumitsu Ogra
Summary: DMTP, a widely used organophosphorus pesticide, has been detected in clinical cases of accidental exposure and suicide. This study found that DMTP forms adducts with specific residues of human serum albumin, which can serve as biomarkers for DMTP poisoning.
JOURNAL OF TOXICOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yasunori Fukumoto, Kemmu Matsuhashi, Yu-ki Tanaka, Noriyuki Suzuki, Yasumitsu Ogra
Summary: Selenium is an essential element in animals but can be highly toxic when consumed above the recommended levels. This study investigates the mechanisms that determine cellular susceptibility to selenite exposure. The findings suggest that the expression level of SLC4A1 is the key determinant of cellular sensitivity to selenite.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
