Article
Biochemistry & Molecular Biology
Nasir Javaid, Thuong L. H. Pham, Sangdun Choi
Summary: Reversing the transcriptional status of genes using CRISPR/dCas9-based activators has proven to be useful for various purposes. Our study demonstrates that dCas9-VP192 is more effective than VP64-dCas9-VP64 in activating reference genes, resulting in higher mRNA and protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xuelian Cui, Chao Zhang, Zhifang Xu, Shuaibin Wang, Xin Li, Erica Stringer-Reasor, Sejong Bae, Leiping Zeng, Dehua Zhao, Runhua Liu, Lei S. Qi, Lizhong Wang
Summary: This study proposes a potential therapy for female breast cancer by reactivating non-mutated allele and silencing XIST gene from X-chromosome inactivation. The simultaneous activation and repression of FOXP3 and XIST genes showed promising results in inhibiting tumor growth in vitro and in vivo.
Article
Medicine, Research & Experimental
Chao Liu, Ning Wang, Rui Luo, Lu Li, Wen Yang, Xiye Wang, Meiling Shen, Qinjie Wu, Changyang Gong
Summary: The study presented a novel nanoCRISPR, named PICASSO, that efficiently activates tumor endogenous genes for effective anti-tumor therapy. By targeting endogenous genes in tumor cells, the therapy can inhibit tumor growth effectively, overcoming the limitations of gene size and type.
Article
Biochemical Research Methods
Zhijie Qin, Yutong Yang, Shiqin Yu, Li Liu, Yue Chen, Jian Chen, Jingwen Zhou
Summary: A CRISPRi system based on the endogenous CRISPR/Cas system in Gluconobacter oxydans was developed, showing effective multiplex gene repression. The study further investigated the role of central carbon metabolism pathways and demonstrated potential metabolic engineering applications, enriching gene regulation tools and promoting performance improvements in G. oxydans.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Mei-Hwa Lee, James L. Thomas, Chien-Yu Lin, Yi-Chen Ethan Li, Hung-Yin Lin
Summary: A CRISPRa transcription activation system was used to upregulate insulin expression in HEK293T cells. Magnetic chitosan nanoparticles, imprinted with a peptide from the Cas9 protein, were developed to increase the delivery of the targeted CRISPR/dCas9a and bind to dCas9a complexed with a guide RNA (gRNA). The adsorption of dCas9 proteins conjugated with activators (SunTag, VPR, and p300) to the nanoparticles was monitored, and the nanoparticles were used to deliver dCas9a complexed with a synthetic gRNA into HEK293T cells to activate their insulin gene expression.
Article
Cell Biology
Phoebe C. R. Parrish, James D. Thomas, Austin M. Gabel, Shriya Kamlapurkar, Robert K. Bradley, Alice H. Berger
Summary: The use of pgPEN method has led to the discovery of important cancer vulnerabilities and synthetic lethal paralog pairs, which can potentially guide the selection of drug targets and provide insights into genetic interactions in cancer.
Article
Biochemical Research Methods
Fabian Machens, Guangyao Ran, Ciaran Ruehmkorff, Julie Meyer auf der Heyde, Bernd Mueller-Roeber, Lena Hochrein
Summary: Metabolic engineering approaches often require modulation of host gene expression in addition to fine-tuning heterologous genes. In this study, a programmable red light switch called PhiReX 2.0 is introduced, which can rewire metabolic fluxes by targeting endogenous promoter sequences using single-guide RNAs (sgRNAs) and activating gene expression in Saccharomyces cerevisiae upon red light stimulation. This design offers the advantage of efficient and straightforward sgRNA exchange using Golden Gate-based cloning, allowing for rational or randomized combination of up to four sgRNAs in a single expression array. Additionally, target gene expression can be rapidly upregulated by short red light pulses in a light dose-dependent manner and returned to the native expression level by applying far-red light without interfering with the cell culture.
ACS SYNTHETIC BIOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Angela Lek, Kaiyue Ma, Keryn G. Woodman, Monkol Lek
Summary: CRISPR-based technology has been widely used for genome modifications and transcription regulation, with a focus on targeted gene activation. Studies have utilized CRISPR-based activation for understanding biological mechanisms, disease correction, and high-throughput genotype-phenotype mapping in genetic screens. Key considerations for potential clinical translation of CRISPR-based activation technology have been explored.
HUMAN GENE THERAPY
(2021)
Article
Multidisciplinary Sciences
Yu Fu, Zhenglin Zhu, Geng Meng, Rijun Zhang, Yueping Zhang
Summary: Post-translational modifications of histone proteins play a crucial role in gene expression and cell fate decisions. Developing efficient methods for introducing mutations to histones is vital for studying these modifications. By utilizing a CRISPR-Cas9 based system in yeast cells, researchers successfully generated multiple histone mutants, presenting a valuable tool for the field of epigenetics.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Sara M. Klose, Nadeeka Wawegama, Fiona M. Sansom, Marc S. Marenda, Glenn F. Browning
Summary: In this study, the researchers used the Mycoplasma gallisepticum CRISPR/Cas system to targeted mutate a nuclease gene, providing insights into the role of this gene and revealing the effectiveness of the system in interfering with gene functions.
VETERINARY MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Matthew L. Bendall, Jasmine H. Francis, Alexander N. Shoushtari, Douglas F. Nixon
Summary: The study identified four HERV-based subgroups in uveal melanoma, providing clear delineation of risk outcome and supporting other molecular subtypes. Intergenic HERVs with coding potential may represent potential therapeutic targets in tumor classification.
Article
Biotechnology & Applied Microbiology
Ling Liu, Danlu Yang, Zhiyu Zhang, Tao Liu, Guoquan Hu, Mingxiong He, Shumiao Zhao, Nan Peng
Summary: This study developed a high-efficiency endogenous CRISPR-Cas system-based genome editing tool for Pediococcus acidilactici, allowing for various genetic manipulations. The engineered strain edited by this tool showed significant advantages in cell growth and lactic acid fermentation, making it a sophisticated chassis species for synthetic biology and bioindustry.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yu-Fan Chuang, Andrew J. Phipps, Fan-Li Lin, Valerie Hecht, Alex W. Hewitt, Peng-Yuan Wang, Guei-Sheung Liu
Summary: The CRISPR/Cas system is a groundbreaking genetic technology that allows gene modification by targeting specific genomic sites. Despite its versatility, the translation of CRISPR/Cas system still faces hurdles that need to be overcome, especially in improving delivery efficiency for potential applications in various fields.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Shujie Gu, Jie Zhang, Lili Li, Jin Zhong
Summary: This study established the first endogenous CRISPR-Cas9 genome-editing system in lactobacilli, achieving efficient gene editing, deletion of large genomic fragments, and simultaneous deletion of multiple genes. A controllable plasmid-targeting sgRNA expression module was designed for high-efficiency gene deletion and plasmid curing.
ACS SYNTHETIC BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Elvir Becirovic
Summary: The CRISPR-Cas technology has been extended beyond its original function as genetic scissors, and the gene-activating CRISPR-Cas modules (CRISPRa) offer advantages such as low off-target effects and independence of target gene size, making them attractive for therapeutic applications.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Reproductive Biology
C. Herrera, M. I. Morikawa, C. Baca Castex, M. R. Pinto, N. Ortega, T. Fanti, R. Garaguso, M. J. Franco, M. Castanares, C. Castaneira, L. Losinno, M. H. Miragaya, A. A. Mutto
Review
Veterinary Sciences
Marc Maserati, Adrian Mutto
JOURNAL OF EQUINE VETERINARY SCIENCE
(2016)
Article
Agriculture, Dairy & Animal Science
German G. Kaiser, Nicolas C. Mucci, Vega Gonzalez, Lourdes Sanchez, Jose A. Parron, Maria D. Perez, Miguel Calvo, Juan F. Alter, Federico A. Hozbor, Adrian A. Mutto
JOURNAL OF DAIRY SCIENCE
(2017)
Article
Veterinary Sciences
Ana Ines Marinone, Nicolas Mucci, German Kaiser, Luis Losinno, Joaquin Armendano, Edgardo Rodriguez, Adrian Mutto, Cecilia Redolatti, Sofia Cantatore, Marcela Fernanda Herrera, Juan Manuel Herrera, Elida Fumuso
JOURNAL OF EQUINE VETERINARY SCIENCE
(2017)
Article
Developmental Biology
Carlos Agustin I. Alonso, Claudia E. Osycka-Salut, Luciana Castellano, Andreina Cesari, Nicolas Di Siervi, Adrian Mutto, Anders Johannisson, Jane M. Morrell, Carlos Davio, Silvina Perez-Martinez
MOLECULAR HUMAN REPRODUCTION
(2017)
Article
Multidisciplinary Sciences
Maria Jesus Canepa, Nicolas Matias Ortega, Melisa Carolina Monteleone, Nicolas Mucci, German Gustavo Kaiser, Marcela Brocco, Adrian Mutto
Review
Multidisciplinary Sciences
Gustavo Adolfo Palma, Martin Eduardo Arganaraz, Antonio Daniel Barrera, Daniela Rodler, Adrian Angel Mutto, Fred Sinowatz
SCIENTIFIC WORLD JOURNAL
(2012)
Article
Reproductive Biology
Janet F. Roser, Maria V. Etcharren, Marcelo H. Miragaya, Adrian Mutto, Mark Colgin, Luis Losinno, Pablo J. Ross
Article
Reproductive Biology
A. Cesari, G. G. Kaiser, N. Mucci, A. Mutto, A. Vincenti, M. W. Fornes, R. H. Alberio
Article
Parasitology
AA Mutto, S Giambiaggi, SO Angel
VETERINARY PARASITOLOGY
(2006)