Journal
NUTRIENTS
Volume 13, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/nu13072332
Keywords
garlic; multimethodological evaluation; CIEL*a*b*; HS-SPME/GC-MS; oxidative stress; inflammation; bioinformatics
Categories
Funding
- il Grappolo S.r.l. 2020 (Soliera, Modena, Italy)
Ask authors/readers for more resources
The study aimed to investigate the protective effects of a garlic hydroalcoholic extract on mouse heart specimens exposed to E. coli lipopolysaccharide-induced oxidative stress and inflammation. The extract was found to inhibit various inflammatory and oxidative stress pathways, demonstrating cardioprotective effects.
The aim of the present study was to investigate the possible protective effects of a garlic hydroalcoholic extract on the burden of oxidative stress and inflammation occurring on mouse heart specimens exposed to E. coli lipopolysaccharide (LPS), which is a well-established inflammatory stimulus. Headspace solid-phase microextraction combined with the gas chromatography-mass spectrometry (HS-SPME/GC-MS) technique was applied to determine the volatile fraction of the garlic powder, and the HS-SPME conditions were optimized for each of the most representative classes of compounds. CIEL*a*b* colorimetric analyses were performed on the powder sample at the time of delivery, after four and after eight months of storage at room temperature in the dark, to evaluate the color changing. Freshly prepared hydroalcoholic extract was also evaluated in its color character. Furthermore, the hydroalcoholic extract was analyzed through GC-MS. The extract was found to be able to significantly inhibit LPS-induced prostaglandin (PG) E-2 and 8-iso-PGF(2 alpha) levels, as well as mRNA levels of cyclooxygenase (COX)-2, interleukin (IL)-6, and nuclear factor-kB (NF-kB), in heart specimens. Concluding, our findings showed that the garlic hydroalcoholic extract exhibited cardioprotective effects on multiple inflammatory and oxidative stress pathways.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available