Article
Biotechnology & Applied Microbiology
Jiawei Zhou, Jiayan Wang, Yuanlu Zhou, Kaiqiang Liu, Yuele Lu, Linjiang Zhu, Xiaolong Chen
Summary: Microbial interactions are crucial for the formation, stabilization, and performance of natural microbial communities. However, little is known about the specific interactions that lead to the establishment of stable microbial communities. This study focused on the solid-state fermentation of Jiangqu, a naturally-formed thick broad-bean sauce, to investigate its microbial diversity and batch stability. The dominant microbial strains, including Aspergillus oryzae, Bacillus, Staphylococcus, and Weissella, were identified, and their interactions during fermentation were characterized. The findings highlight the importance of direct adsorption and competition as key interactions in solid-state fermentation, contrasting with liquid cocultivation methods.
Article
Geriatrics & Gerontology
Yuwei Liu, Li Peng, Jing Chen, Ling Chen, Ying Wu, Mengxin Cheng, Min Chen, Xujun Ye, Yalei Jin
Summary: This study found that EIF5A2 can alleviate cell senescence by regulating UPR-related genes via specific transcription factors, expanding the current understanding of the mechanisms of EIF5A2-mediated gene regulation.
Article
Biotechnology & Applied Microbiology
Marcos Francisco Perez, Peter Sarkies
Summary: The study reveals weak and inconsistent correlations between mitochondrial and nuclear-encoded mitochondrial genes in most healthy human tissues, with a subset of tissues showing significant coordination in high NF-κB expression. In contrast, most cancer types exhibit robust coordination of nuclear and mitochondrial OXPHOS gene expression, suggesting this as a feature of gene regulation in cancer.
Article
Cell Biology
Sara Correia Santos, Thorsten Bischler, Alexander J. Westermann, Joerg Vogel
Summary: The study reveals the important role of the Hfq-associated sRNA PinT in Salmonella virulence, showing its translational inhibition of the secreted effector kinase SteC and delay of actin rearrangements in infected host cells. The findings highlight the need for complementary methods to uncover the full target suites of sRNAs.
Review
Microbiology
Mizuki Tanaka, Katsuya Gomi
Summary: The filamentous fungus Aspergillus oryzae, also known as yellow koji mold, produces high levels of hydrolases such as amylolytic and proteolytic enzymes. The expression of hydrolase genes in A. oryzae is induced by certain substrates and regulated by carbon catabolite repression.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Temoor Ahmed, Luqiong Lv, Muhammad Noman, Hafiza Ayesha Masood, Muhammad Rizwan, Munazza Ijaz, Ashraf Atef Hatamleh, Munirah Abdullah Al-Dosary, Hayssam M. Ali, Jianping Chen, Bin Li
Summary: In this study, the toxicity and molecular action mechanisms of bioengineered chitosan-iron nanocomposites (BNCs) against the bacterial leaf blight pathogen were investigated using transcriptomic and proteomic approaches. The results showed that BNCs inhibited various processes related to cell membrane biosynthesis, antioxidant stress, DNA damage, flagellar biosynthesis, and transcriptional regulation. The study suggests that BNCs have the potential to serve as an eco-friendly and sustainable alternative for controlling bacterial leaf blight in rice.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Plant Sciences
Bin Qian, Xiaotong Su, Ziyuan Ye, Xinyu Liu, Muxing Liu, Danyu Shen, Han Chen, Haifeng Zhang, Ping Wang, Zhengguang Zhang
Summary: During interactions between plant-pathogenic fungi and host plants, the COPII cargo receptor MoErv29 serves as a target of the transcription factor MoHac1, crucial for efficient delivery of secreted proteins. Deletion of MoERV29 in Magnaporthe oryzae significantly impairs vegetative growth, conidiation, and biotrophic invasion in susceptible rice hosts. By recognizing and binding amino-terminal tripeptide motifs, MoErv29 is essential for the secretion of various proteins, including laccases and effectors important for host recognition and disease resistance.
Article
Biology
Mizuki Tanaka, Silai Zhang, Shun Sato, Jun-ichi Yokota, Yuko Sugiyama, Yasuaki Kawarasaki, Youhei Yamagata, Katsuya Gomi, Takahiro Shintani
Summary: This study demonstrates the occurrence of Regulated Ire1-dependent decay (RIDD) in eukaryotic microorganisms under physiological endoplasmic reticulum (ER) stress. RIDD was observed in filamentous fungus using Aspergillus oryzae as a model, and it was triggered by inducing amylase production without any artificial ER stress inducer.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yanyan Wang, Xiaowei Li, Xin Chen, Jens Nielsen, Dina Petranovic, Verena Siewers
Summary: Monoclonal antibodies, antibody fragments, and fusion proteins have revolutionized medicine, but challenges in the biopharmaceutical industry include high production costs and low productivity in mammalian cell lines. Saccharomyces cerevisiae has been used as a secretion host for various products, but due to incompatible surface glycosylation, non-glycosylated antibody fragments need to be chosen. The secretion level of antibody fragments in yeast strains is correlated with their secretory capacity and unique physiological needs.
MICROBIAL CELL FACTORIES
(2021)
Article
Biotechnology & Applied Microbiology
Gobong Choi, Jongbum Jeon, Hyunjun Lee, Shenxian Zhou, Yong-Hwan Lee
Summary: This study identified novel lncRNAs during host infection of the model pathogenic fungus Magnaporthe oryzae and revealed their potential roles in pathogenesis-related gene regulation.
Editorial Material
Cell Biology
Giulia Cerrato, Oliver Kepp, Allan Sauvat, Guido Kroemer
Summary: Oleate blocks conventional protein secretion and induces the local enrichment of LC3 at the trans-Golgi network, independent of the recruitment of LC3 to the Golgi apparatus.
Article
Endocrinology & Metabolism
Paul G. Weightman Potter, Sam J. Washer, Aaron R. Jeffries, Janet E. Holley, Nick J. Gutowski, Emma L. Dempster, Craig Beall
Summary: The study found that exposure of human astrocytes to transient low glucose triggers activation of genes related to endoplasmic reticulum stress, while after recurrent low glucose exposure, the activation of stress-related genes decreases, suggesting attenuated endoplasmic reticulum stress. This may be a result of successful metabolic adaptation, better preserving intracellular energy levels.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Microbiology
Pui Shan Wong, Koichi Tamano, Sachiyo Aburatani
Summary: The study identified vital pathways and genes for FFA secretory productivity in A. oryzae, and through gene modification, key genes were found to significantly increase FFA secretory productivity. Modifying these genes can lead to a significant increase in FFA secretion productivity, providing important guidance for further research and application of metabolic engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Venura Herath, Jeanmarie Verchot
Summary: In this study, potato plantlets were treated with TM and gene expression studies were performed to identify genome-wide changes associated with ER stress and the UPR. An extensive network of responses was identified, including chromatin remodeling, transcriptional reprogramming, as well as changes in the structural components of the endomembrane network system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Vishesh Kumar, Priyanka Jain, Sureshkumar Venkadesan, Suhas Gorakh Karkute, Jyotika Bhati, Malik Zainul Abdin, Amitha Mithra Sevanthi, Dwijesh Chandra Mishra, Krishna Kumar Chaturvedi, Anil Rai, Tilak Raj Sharma, Amolkumar U. Solanke
Summary: This study analyzed the transcriptome of rice plants in response to panicle blast infection, identifying key genes involved in defense response, cell wall strengthening pathways, and potential mechanisms of panicle blast resistance. Understanding these mechanisms could aid in breeding resistant rice varieties.
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.