Smartphone-based technology for nanomolecular detection of aflatoxin B1 by aptamer-conjugated magnetic nanoparticles

The need for a healthy market in the rapid and accurate screening of a variety of pathogenic agents and toxins in the environment and food has led to an increase in the development of new biosensors, which have ideal characteristics, such as high sensitivity and specificity with rapid detection and simple preparation of the sample. Among the food contaminants, mycotoxins have been identified as a major challenge for the food industry, and rapid and accurate detection has attracted the attention of food inspection and monitoring organisations. In this study, a nanomolecular detection method is described using aflatoxin B-1(AFB(1))-specific aptamers attached to streptavidin-coated magnetic nanoparticles. A prominent feature of the AFB(1)-specific aptamers is a guanine-rich (G-rich) sequence with a G-quadruplex structure after capturing AFB(1) molecules and mimicking peroxidase activity. The enzymatic reaction evaluated in the presence of chromogenic substrate and measurement is done by a smartphone-specific application for colorimetric measurement. The results indicated that the assay could measure AFB(1) in rice, flour, seed, maize, and pistachio. In addition, the application of hybrid nanomaterial technology resulting from the binding of biotin-labelled aptamers to the surface of streptavidin-coated magnetic nanoparticles minimises preparation and treatment of samples, improves results, and consequently reduces false negative and positive responses in the detection field. This study may eventually lead to the design and development of a fast, sensitive, specific, and on-site AFB(1)-based nanomolecular colorimetric detection system via a smartphone-based application that can be readily accessible to all applicants, from professionals to manufacturers of foodstuffs.

Automated summary

This study presents a nanomolecular detection method for rapid and accurate detection of aflatoxin B-1 (AFB(1)) in food using specific aptamers attached to magnetic nanoparticles. The method utilizes a smartphone application for colorimetric measurement. The results show that the method is effective for detecting AFB(1) in rice, flour, seed, maize, and pistachio, with minimal sample preparation.