Article
Biochemistry & Molecular Biology
Hitomi Ohinata, Takashi Obama, Tomohiko Makiyama, Yuichi Watanabe, Hiroyuki Itabe
Summary: This study found that high-density lipoproteins (HDL) partially decreased neutrophil extracellular trap (NET) formation induced by copper-oxidized low-density lipoprotein (oxLDL). It was also discovered that oxidized phosphatidylcholines and lysophosphatidylcholine in oxLDL promoted NET formation, which could be completely blocked by native HDL. Furthermore, an electronegative subfraction of LDL, LDL(-), was found to promote NET formation. These findings suggest that plasma lipoproteins and their oxidative modifications play multiple roles in promoting NET formation, and HDL acts as a suppressor of this response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Hyun-Ki Kim, Jinyoung Hong, Sunyoung Ahn, Woochang Lee, Sail Chun, Won-Ki Min
Summary: The study found that in the general population, high levels of sdLDL-C are associated with high levels of oxLDL. However, the applicability of the equation for estimating sdLDL-C developed from the general population is limited in patients receiving DM treatment.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Chemistry, Analytical
Soemwit Khongwichit, Piyawut Swangphon, Natthaphon Nanakorn, Aekkaraj Nualla-ong, Kiattawee Choowongkomon, Peter A. Lieberzeit, Suticha Chunta
Summary: In this study, a simple colorimetric assay was developed for sensitive and specific determination of oxidized low-density lipoprotein (oxLDL) using a selective aptamer coupled with salt-induced gold nanoparticle aggregation. The assay showed a linear response to oxLDL in the range of 0.002 to 0.5 μmol/L, with high selectivity and accurate recovery. When applied to artificial serum, the assay exhibited excellent correlation with conventional thiobarbituric acid reactive substances (TBARS), providing a rapid and convenient alternative for oxLDL detection.
Review
Cardiac & Cardiovascular Systems
Alexander Akhmedov, Tatsuya Sawamura, Chu-Huang Chen, Simon Kraler, Daria Vdovenko, Thomas F. Luscher
Summary: LOX-1, as a scavenger receptor, plays a crucial role in the development of atherosclerosis, with implications in various cell types, making it a promising diagnostic and therapeutic target for certain cardiovascular diseases.
EUROPEAN HEART JOURNAL
(2021)
Review
Cardiac & Cardiovascular Systems
Alexander Akhmedov, Tatsuya Sawamura, Chu-Huang Chen, Simon Kraler, Daria Vdovenko, Thomas F. Luescher
Summary: LOX-1, a scavenger receptor that promotes atherosclerotic plaque formation, plays a critical role in the development of cardiovascular diseases. Its expression in various cell types underscores its significance in disease progression, highlighting the potential for LOX-1 as a novel therapeutic target in CVDs prevention and treatment.
EUROPEAN HEART JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Jonathan S. Rink, Adam Yuh Lin, Kaylin M. McMahon, Andrea E. Calvert, Shuo Yang, Tim Taxter, Jonathan Moreira, Amy Chadburn, Amir Behdad, Reem Karmali, C. Shad Thaxton, Leo Gordon
Summary: Recent research suggests that malignant cells dependent on cholesterol uptake from lipoproteins are susceptible to ferroptosis, a type of cell death triggered by accumulation of oxidized lipids in cell membranes. Treatment of these cells with HDL NPs activates a compensatory metabolic response, leading to increased de novo cholesterol synthesis and downregulation of GPX4 expression, resulting in ferroptosis-mediated cell death. In vivo studies in mouse lymphoma xenografts and patient samples further support the potential of targeting SCARB1 with HDL NPs for cholesterol uptake-addicted lymphoma cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Xu Zhi, Yufen Sun, Fangjian Cai, Sisi Wang, Hu Gao, Fan Wu, Lei Zhang, Zhen Shen
Summary: A highly specific Ox-LDL-triggered fluorogenic-colorimetric probe Pro-P1 has been developed to visualize the oxidation and aggregation progress of lipoproteins and plaque. This probe is able to directly visualize ferroptosis and Cu2+-mediated LDL oxidative aggregation in living cells. The plaque area and size can be delineated by colorimetric fluorescence imaging and fluorescence lifetime imaging.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Hanlin Peng, Mingzhu Zhu, Wei Kong, Chaoshu Tang, Junbao Du, Yaqian Huang, Hongfang Jin
Summary: The results demonstrate that L-Cth exerts inhibitory effects on oxidative stress by reducing superoxide anion, H2O2, and NO levels, as well as iNOS protein expression induced by oxLDL. Additionally, L-Cth treatment significantly enhances the activity of antioxidants GSH-Px, SOD, and CAT, and upregulates the expressions of SOD1 and SOD2 proteins in oxLDL-treated macrophages.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Hui-Lin Hu, Hui-Xiu Zheng, Na Yuan, Chang-Lin Zhai, Hao Chen, Hai-Hua Pan, Gang Qian
Summary: circUSP9X promotes the proliferation and migration of oxidized low-density lipoprotein-treated vascular smooth muscle cells by regulating the expression of miR-599 and STIM1.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2023)
Article
Surgery
Halit Eren Taskin, Ahmet Kocael, Pinar Kocael, Kagan Zengin, Muzaffer Al, Volkan Sozer, J. N. Buchwald, T. W. McGlennon, Hafize Uzun
Summary: The study found that weight loss induced by sleeve gastrectomy (SG) can improve the cardiovascular conditions of patients with morbid obesity. After 12 months of SG, metabolic and inflammatory biomarkers significantly decreased and approached the levels of the control group. Baseline albumin level as well as the reduction in oxLDL and cholesterol retention fraction (CRF) were correlated with the reduction in sLOX-1.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Biochemistry & Molecular Biology
Yamei Li, Xueya Zhao, Biwei He, Weibin Wu, Huijuan Zhang, Xingyu Yang, Weiwei Cheng
Summary: Autophagy plays a crucial role in maintaining the function of trophoblast cells, with its activation under hypoxia enhancing angiogenesis and protecting against apoptosis, potentially offering a preventive approach for early-onset preeclampsia.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lina Wirestam, Frida Jonsson, Helena Enocsson, Christina Svensson, Maria Weiner, Jonas Wettero, Helene Zachrisson, Per Eriksson, Christopher Sjowall
Summary: A study examined the association between anti-oxidized low-density lipoprotein (anti-oxLDL) antibodies and subclinical atherosclerosis in patients with different systemic lupus erythematosus (SLE) phenotypes. Anti-oxLDL levels were measured in SLE patients, healthy controls, and subjects with anti-neutrophil cytoplasmic antibody-associated vasculitis (AAV). The results showed no significant difference in anti-oxLDL levels between SLE patients and healthy controls, but levels were significantly higher in AAV subjects. There was also a significant decrease in anti-oxLDL levels in the SLE group over a 3-year period. The study did not find strong associations between anti-oxLDL antibodies and vascular affection in SLE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Kun Wang, Chaoye Gan, Huaying Wang, Meiying Ao, Youlong Fan, Yong Chen
Summary: Research shows that acidification causes changes in size and biomechanical properties of LDL/oxLDL particles, with significant decreases in hardness, potentially explaining their role in atherosclerosis.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Gastroenterology & Hepatology
Shi-Min Zheng, Hao Chen, Wei-Hong Sha, Xiao-Fen Chen, Jian-Bin Yin, Xiao-Bo Zhu, Zhong-Wen Zheng, Juan Ma
Summary: ox-LDL stimulates CD206+ macrophages to upregulate CD44 and CD133 expression in HFD related CRC, suggesting a potential role of ox-LDL in the development of HFD associated CRC.
WORLD JOURNAL OF GASTROENTEROLOGY
(2022)
Article
Oncology
Jesus S. Aguilar Diaz De Leon, Honor L. Glenn, Mark Knappenberger, Chad R. Borges
Summary: The oxidation and desialylation of LDL in atherosclerotic processes significantly affect the cytotoxicity of LAK cells against K562 cells.