Journal
VIRUSES-BASEL
Volume 13, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/v13091848
Keywords
bacteriophage; genome sequencing; endolysin; antimicrobial activity; Acinetobacter baumannii
Categories
Funding
- Korea Government National Research Foundation [2016R1D1A1B01008960]
- National Research Foundation of Korea [2016R1D1A1B01008960] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Acinetobacter baumannii is a nosocomial pathogen worldwide, posing a challenge due to the emergence of multidrug-resistant strains. A novel bacteriophage, phi Ab1656-2, was isolated from clinical A. baumannii 1656-2, showing potential as a therapeutic agent against MDRAB. Its endolysin, AbEndolysin, demonstrated significant antibacterial activity against MDRAB clinical strains, suggesting it as a potential antimicrobial agent.
Acinetobacter baumannii is a nosocomial pathogen, which is a problem worldwide due to the emergence of a difficult-to-treat multidrug-resistant A. baumannii (MDRAB). Endolysins are hydrolytic enzymes produced by a bacteriophage that can be used as a potential therapeutic agent for multidrug-resistant bacterial infection in replacing antibiotics. Here, we isolated a novel bacteriophage through prophage induction using mitomycin C from clinical A. baumannii 1656-2. Morphologically, phi Ab1656-2 was identified as a Siphoviridae family bacteriophage, which can infect MDRAB. The whole genome of phi Ab1656-2 was sequenced, and it showed that it is 50.9 kb with a G + C content of 38.6% and 68 putative open reading frames (ORFs). A novel endolysin named AbEndolysin with an N-acetylmuramidase-containing catalytic domain was identified, expressed, and purified from phi Ab1656-2. Recombinant AbEndolysin showed significant antibacterial activity against MDRAB clinical strains without any outer membrane permeabilizer. These results suggest that AbEndolysin could represent a potential antimicrobial agent for treating MDRAB clinical isolates.
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