Engineering insects from the endosymbiont out

Genetic Modification of Sodalis Species by DNA Transduction

(2021) Chelsea M. Keller et al.   mSphere

Vertical Transmission at the Pathogen-Symbiont Interface: Serratia symbiotica and Aphids

(2021) Julie Perreau et al.   mBio

Microorganisms in the reproductive tissues of arthropods

(2020) Jessamyn I. Perlmutter et al.   NATURE REVIEWS MICROBIOLOGY

Engineered symbionts activate honey bee immunity and limit pathogens

(2020) Sean P. Leonard et al.   SCIENCE

Transformation of the Drosophila sex-manipulative endosymbiont Spiroplasma poulsonii and persisting hurdles for functional genetics studies

(2020) Florent Masson et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Burkholderia insecticola triggers midgut closure in the bean bug Riptortus pedestris to prevent secondary bacterial infections of midgut crypts

(2020) Yoshitomo Kikuchi et al.   ISME Journal

Developing a new class of engineered live bacterial therapeutics to treat human diseases

(2020) Mark R. Charbonneau et al.   Nature Communications

Innovations to culturing the uncultured microbial majority

(2020) William H. Lewis et al.   NATURE REVIEWS MICROBIOLOGY

Metagenomic engineering of the mammalian gut microbiome in situ

(2019) Carlotta Ronda et al.   NATURE METHODS

Green, Yellow, and Red Fluorescent Proteins as Markers for Bacterial Isolates from Mosquito Midguts

(2019) Ephantus Muturi et al.   Insects

Genetic manipulation allows in vivo tracking of the life cycle of the son‐killer symbiont, Arsenophonus nasoniae , and reveals patterns of host invasion, tropism and pathology

(2019) Pol Nadal‐Jimenez et al.   ENVIRONMENTAL MICROBIOLOGY

Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta

(2019) Flavia MM Bento et al.   PEST MANAGEMENT SCIENCE

Versatile and Dynamic Symbioses Between Insects and Burkholderia Bacteria

(2019) Martin Kaltenpoth et al.   Annual Review of Entomology

Host–symbiont specificity determined by microbe–microbe competition in an insect gut

(2019) Hideomi Itoh et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification and validation of potential reference gene for effective dsRNA knockdown analysis in Chilo partellus

(2019) Olawale Samuel Adeyinka et al.   Scientific Reports

Mapping and editing animal mitochondrial genomes: can we overcome the challenges?

(2019) Anna Klucnika et al.   PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

CRISPR/Cas9-mediated gene deletion of the ompA gene in symbiotic Cedecea neteri impairs biofilm formation and reduces gut colonization of Aedes aegypti mosquitoes

(2019) Shivanand Hegde et al.   PLoS Neglected Tropical Diseases

How Many Species of Insects and Other Terrestrial Arthropods Are There on Earth?

(2018) Nigel E. Stork   Annual Review of Entomology

Using Wolbachia for Dengue Control: Insights from Modelling

(2018) Ilaria Dorigatti et al.   TRENDS IN PARASITOLOGY

Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids

(2018) Sean P. Leonard et al.   ACS Synthetic Biology

A paratransgenic strategy to block transmission of Xylella fastidiosa from the glassy-winged sharpshooter Homalodisca vitripennis

(2018) Arinder K. Arora et al.   BMC BIOTECHNOLOGY

Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

(2018) Duverney Chaverra-Rodriguez et al.   Nature Communications

Towards improving tsetse fly paratransgenesis: stable colonization of Glossina morsitans morsitans with genetically modified Sodalis

(2018) Linda De Vooght et al.   BMC MICROBIOLOGY

Evidence for Gut-Associated Serratia symbiotica in Wild Aphids and Ants Provides New Perspectives on the Evolution of Bacterial Mutualism in Insects

(2018) François Renoz et al.   MICROBIAL ECOLOGY

Delivery of a Genetically Marked Serratia AS1 to Medically Important Arthropods for Use in RNAi and Paratransgenic Control Strategies

(2018) Mona Koosha et al.   MICROBIAL ECOLOGY

The lipopolysaccharide core oligosaccharide ofBurkholderiaplays a critical role in maintaining a proper gut symbiosis with the bean bugRiptortus pedestris

(2017) Jiyeun Kate Kim et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns

(2017) Clauvis Nji Tizi Taning et al.   JOURNAL OF INSECT PHYSIOLOGY

Optimization of recombinant bacteria expressing dsRNA to enhance insecticidal activity against a lepidopteran insect, Spodoptera exigua

(2017) Mohammad Vatanparast et al.   PLoS One

Driving mosquito refractoriness toPlasmodium falciparumwith engineered symbiotic bacteria

(2017) Sibao Wang et al.   SCIENCE

Antibiotic-producing symbionts dynamically transition between plant pathogenicity and insect-defensive mutualism

(2017) Laura V. Flórez et al.   Nature Communications

Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

(2016) Katarzyna Michalik et al.   ARTHROPOD STRUCTURE & DEVELOPMENT

Effects of co-occurring Wolbachia and Spiroplasma endosymbionts on the Drosophila immune response against insect pathogenic and non-pathogenic bacteria

(2016) Upasana Shokal et al.   BMC MICROBIOLOGY

Assessment of genetically engineered Trabulsiella odontotermitis as a ‘Trojan Horse’ for paratransgenesis in termites

(2016) Chinmay Vijay Tikhe et al.   BMC MICROBIOLOGY

Isolation and assessment of gut bacteria from the Formosan subterranean termite,Coptotermes formosanus(Isoptera: Rhinotermitidae), for paratransgenesis research and application

(2016) Chinmay V. Tikhe et al.   Insect Science

Genome-based phylogeny and taxonomy of the ‘Enterobacteriales’: proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov.

(2016)   INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

Morganella morganii bacteria produces phenol as the sex pheromone of the New Zealand grass grub from tyrosine in the colleterial gland

(2016) D. G. Marshall et al.   NATURWISSENSCHAFTEN

D-Alanine-Controlled Transient Intestinal Mono-Colonization with Non-Laboratory-Adapted Commensal E. coli Strain HS

(2016) Miguelangel Cuenca et al.   PLoS One

Genome-wide screen identifies host colonization determinants in a bacterial gut symbiont

(2016) J. Elijah Powell et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploiting Intimate Relationships: Controlling Mosquito-Transmitted Disease with Wolbachia

(2016) Eric P. Caragata et al.   TRENDS IN PARASITOLOGY

Colonization of the Intestinal Tract of the Polyphagous Pest Spodoptera littoralis with the GFP-Tagged Indigenous Gut Bacterium Enterococcus mundtii

(2016) Beng-Soon Teh et al.   Frontiers in Microbiology

Morganella morganii bacteria produces phenol as the sex pheromone of the New Zealand grass grub from tyrosine in the colleterial gland

(2016) D. G. Marshall et al.   Science of Nature

A delivery system for field application of paratransgenic control

(2015) Arinder K. Arora et al.   BMC BIOTECHNOLOGY

Paternal Transmission of a Secondary Symbiont during Mating in the Viviparous Tsetse Fly

(2015) Linda De Vooght et al.   MOLECULAR BIOLOGY AND EVOLUTION

Inhibition of Plasmodium berghei Development in Mosquitoes by Effector Proteins Secreted from Asaia sp. Bacteria Using a Novel Native Secretion Signal

(2015) Nicholas J. Bongio et al.   PLoS One

Heritable symbiosis: The advantages and perils of an evolutionary rabbit hole

(2015) Gordon M. Bennett et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Insect’s intestinal organ for symbiont sorting

(2015) Tsubasa Ohbayashi et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reproductive Parasitism: Maternally Inherited Symbionts in a Biparental World

(2015) Gregory D.D. Hurst et al.   Cold Spring Harbor Perspectives in Biology

The paratransgenic potential ofLactobacillus kunkeeiin the honey beeApis mellifera

(2015) A. Rangberg et al.   Beneficial Microbes

Genetically Modifying the Insect Gut Microbiota to Control Chagas Disease Vectors through Systemic RNAi

(2015) Mabel L. Taracena et al.   PLoS Neglected Tropical Diseases

Routes of Acquisition of the Gut Microbiota of the Honey Bee Apis mellifera

(2014) J. Elijah Powell et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Insects Recycle Endosymbionts when the Benefit Is Over

(2014) Aurélien Vigneron et al.   CURRENT BIOLOGY

Acquisition and Structuring of Midgut Bacterial Communities in Gypsy Moth (Lepidoptera: Erebidae) Larvae

(2014) Charles J. Mason et al.   ENVIRONMENTAL ENTOMOLOGY

Delivery of a functional anti-trypanosome Nanobody in different tsetse fly tissues via a bacterial symbiont, Sodalis glossinidius

(2014) Linda De Vooght et al.   Microbial Cell Factories

Engineering reduced evolutionary potential for synthetic biology

(2014) Brian A. Renda et al.   Molecular BioSystems

Mosquitoes rely on their gut microbiota for development

(2014) Kerri L. Coon et al.   MOLECULAR ECOLOGY

Intrasperm vertical symbiont transmission

(2014) K. Watanabe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Genomics and host specialization of honey bee and bumble bee gut symbionts

(2014) W. K. Kwong et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Bacterial Cell Wall Synthesis Gene uppP Is Required for Burkholderia Colonization of the Stinkbug Gut

(2013) J. K. Kim et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Live imaging of symbiosis: spatiotemporal infection dynamics of a GFP-labelledBurkholderiasymbiont in the bean bugRiptortus pedestris

(2013) Yoshitomo Kikuchi et al.   MOLECULAR ECOLOGY

Laboratory adaptedEscherichia coliK-12 becomes a pathogen ofCaenorhabditis elegansupon restoration of O antigen biosynthesis

(2013) Douglas F. Browning et al.   MOLECULAR MICROBIOLOGY

Recombinant Arthrobacter β-1, 3-glucanase as a potential effector molecule for paratransgenic control of Chagas disease

(2013) Christo Jose et al.   Parasites & Vectors

Polyester synthesis genes associated with stress resistance are involved in an insect-bacterium symbiosis

(2013) J. K. Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Comparing constitutive and induced costs of symbiont-conferred resistance to parasitoids in aphids

(2013) Christoph Vorburger et al.   Ecology and Evolution

Fighting malaria with engineered symbiotic bacteria from vector mosquitoes

(2012) S. Wang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cellular mechanism for selective vertical transmission of an obligate insect symbiont at the bacteriocyte-embryo interface

(2012) R. Koga et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lambda Red-Mediated Genetic Modification of the Insect EndosymbiontSodalis glossinidius

(2011) Mauricio H. Pontes et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Green fluorescent protein (GFP)-labeling of enterobacteria associated with fruit flies (Diptera: Tephritidae) and persistence in their natural host Rhagoletis completa Cresson.

(2011) Isabel Martinez-Sañudo et al.   CANADIAN JOURNAL OF MICROBIOLOGY

Lessons from Studying Insect Symbioses

(2011) Angela E. Douglas   Cell Host & Microbe

Extreme genome reduction in symbiotic bacteria

(2011) John P. McCutcheon et al.   NATURE REVIEWS MICROBIOLOGY

Drosophila Microbiome Modulates Host Developmental and Metabolic Homeostasis via Insulin Signaling

(2011) S. C. Shin et al.   SCIENCE

Genetic Transformation of Midgut Bacteria from the Red Imported Fire Ant (Solenopsis invicta)

(2009) Freder Medina et al.   CURRENT MICROBIOLOGY

Asaia, a versatile acetic acid bacterial symbiont, capable of cross-colonizing insects of phylogenetically distant genera and orders

(2009) Elena Crotti et al.   ENVIRONMENTAL MICROBIOLOGY

A single genetic locus in the phytopathogenPantoea stewartiienables gut colonization and pathogenicity in an insect host

(2009) John Stavrinides et al.   ENVIRONMENTAL MICROBIOLOGY

Endosymbiotic Bacteria in Insects: Their Diversity and Culturability

(2009) Yoshitomo Kikuchi   MICROBES AND ENVIRONMENTS

Developmental Control of a Lepidopteran Pest Spodoptera exigua by Ingestion of Bacteria Expressing dsRNA of a Non-Midgut Gene

(2009) Honggang Tian et al.   PLoS One

Hamiltonella defensa, genome evolution of protective bacterial endosymbiont from pathogenic ancestors

(2009) P. H. Degnan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Symbiotic bacteria enable insect to use a nutritionally inadequate diet

(2009) E Akman Gunduz et al.   PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES

Assessment of Gut Bacteria for a Paratransgenic Approach To Control Dermolepida albohirtum Larvae

(2008) G. W. Pittman et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cloning and Heterologous Expression of Insecticidal-Protein-Encoding Genes from Photorhabdus luminescens TT01 in Enterobacter cloacae for Termite Control

(2008) R. Zhao et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Paternal transmission of symbiotic bacteria in malaria vectors

(2008) Claudia Damiani et al.   CURRENT BIOLOGY