Journal
TALANTA
Volume 228, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2021.122174
Keywords
Carbaryl; Copper nanoparticles; Antibodies; Electrochemical immunosensor; Linear sweep anodic stripping voltammetry; Gold-graphite electrode
Categories
Funding
- Tomsk Polytechnic University (TPU)
- Russian Foundation for Basic Research [19-5326001\19]
- Czech Science Foundation (GA CR) [20-01417J]
- State Program RF <> [FSWW2020-0022]
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This study synthesized a hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) for the determination of carbaryl, and successfully developed a novel method for monitoring carbaryl residues in flour samples using an electrochemical immunosensor. The detection range was 0.8-32.3 μg/kg with a limit of detection of 0.08 μg/kg.
A hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive determination of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of the GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the Hap-Car-BSA@CuNPs conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8-32.3 mu g.kg(-1), with a limit of detection 0.08 mu g.kg(-1). The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks, and soil samples.
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