Deciphering CAD : Structure and function of a mega‐enzymatic pyrimidine factory in health and disease

Mechanisms of feedback inhibition and sequential firing of active sites in plant aspartate transcarbamoylase

(2021) Leo Bellin et al.   Nature Communications

Structural basis for the interaction modes of dihydroorotase with the anticancer drugs 5-fluorouracil and 5-aminouracil

(2021) Hong-Hsiang Guan et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

The multienzymatic protein CAD leading the de novo biosynthesis of pyrimidines localizes exclusively in the cytoplasm and does not translocate to the nucleus

(2020) Francisco del Caño-Ochoa et al.   NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS

A Patient With CAD Deficiency Responsive to Uridine and Literature Review

(2020) Ling Zhou et al.   Frontiers in Neurology

Cell-based analysis of CAD variants identifies individuals likely to benefit from uridine therapy

(2020) Francisco del Caño-Ochoa et al.   GENETICS IN MEDICINE

The Cellular Protein CAD is Recruited into Ebola Virus Inclusion Bodies by the Nucleoprotein NP to Facilitate Genome Replication and Transcription

(2020) Janine Brandt et al.   Cells

Uridine treatment restores the CDA II ‐like hematological phenotype in a patient with homozygous mutation in the CAD gene

(2020) Roberta Russo et al.   AMERICAN JOURNAL OF HEMATOLOGY

Expanding the clinical and genetic spectrum of CAD deficiency: an epileptic encephalopathy treatable with uridine supplementation

(2020) Daisy Rymen et al.   GENETICS IN MEDICINE

Triacetyluridine treats epileptic encephalopathy from CAD mutations: a case report and review

(2020) Aliya Frederick et al.   Annals of Clinical and Translational Neurology

Pressure-induced activation of latent Dihydroorotase from Aquifex aeolicus as revealed by high pressure protein crystallography

(2019) Thierry Prangé et al.   FEBS Journal

Crystal structures of monometallic dihydropyrimidinase and the human dihydroorotase domain K1556A mutant reveal no lysine carbamylation within the active site

(2018) Jen-Hao Cheng et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Characterization of the catalytic flexible loop in the dihydroorotase domain of the human multi-enzymatic protein CAD

(2018) Francisco del Caño-Ochoa et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structural Insight into the Core of CAD, the Multifunctional Protein Leading De Novo Pyrimidine Biosynthesis

(2017) María Moreno-Morcillo et al.   STRUCTURE

A New View into the Regulation of Purine Metabolism: The Purinosome

(2017) Anthony M. Pedley et al.   TRENDS IN BIOCHEMICAL SCIENCES

CADmutations and uridine-responsive epileptic encephalopathy

(2016) Johannes Koch et al.   BRAIN

Structure and Functional Characterization of Human Aspartate Transcarbamoylase, the Target of the Anti-tumoral Drug PALA

(2016) Alba Ruiz-Ramos et al.   STRUCTURE

Biallelic mutations in CAD, impair de novo pyrimidine biosynthesis and decrease glycosylation precursors

(2015) B. G. Ng et al.   HUMAN MOLECULAR GENETICS

Creation of a putative third metal binding site in type II dihydroorotases significantly enhances enzyme activity

(2015) Yen-Hua Huang et al.   PROTEIN AND PEPTIDE LETTERS

Structure of human carbamoyl phosphate synthetase: deciphering the on/off switch of human ureagenesis

(2015) Sergio de Cima et al.   Scientific Reports

Rheb Protein Binds CAD (Carbamoyl-phosphate Synthetase 2, Aspartate Transcarbamoylase, and Dihydroorotase) Protein in a GTP- and Effector Domain-dependent Manner and Influences Its Cellular Localization and Carbamoyl-phosphate Synthetase (CPSase) Activity

(2014) Tatsuhiro Sato et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the aspartate transcarbamoylase domain of human CAD

(2013) Alba Ruiz-Ramos et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Intersubunit communication in the dihydroorotase-aspartate transcarbamoylase complex ofAquifex aeolicus

(2013) Hedeel Guy Evans et al.   PROTEIN SCIENCE

Stimulation of de Novo Pyrimidine Synthesis by Growth Signaling Through mTOR and S6K1

(2013) I. Ben-Sahra et al.   SCIENCE

Quantitative Phosphoproteomics Reveal mTORC1 Activates de Novo Pyrimidine Synthesis

(2013) A. M. Robitaille et al.   SCIENCE

Structure, Functional Characterization, and Evolution of the Dihydroorotase Domain of Human CAD

(2013) Araceli Grande-García et al.   STRUCTURE

Expression, purification, crystallization and preliminary X-ray diffraction analysis of the dihydroorotase domain of human CAD

(2012) Nada Lallous et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Structure and Mechanisms of Escherichia coli Aspartate Transcarbamoylase

(2011) William N. Lipscomb et al.   ACCOUNTS OF CHEMICAL RESEARCH

TheLeishmania donovaniUMP Synthase Is Essential for Promastigote Viability and Has an Unusual Tetrameric Structure That Exhibits Substrate-controlled Oligomerization

(2011) Jarrod B. French et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Dihydroorotase from the HyperthermophileAquifiex aeolicusIs Activated by Stoichiometric Association with Aspartate Transcarbamoylase and Forms a One-Pot Reactor for Pyrimidine Biosynthesis†‡

(2009) Pengfei Zhang et al.   BIOCHEMISTRY