Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 118, Issue 36, Pages -Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2106943118
Keywords
photoacoustic imaging; copper; Wilson's disease
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Funding
- Alfred P. Sloan Foundation
- Pines Graduate Fellowship
- Roy J. Carver Charitable Trust [15-4521]
- NSF, Division of Biological Infrastructure [DBI-0100085]
- NSF [1752879]
- Division Of Chemistry
- Direct For Mathematical & Physical Scien [1752879] Funding Source: National Science Foundation
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The development of the high-performance photoacoustic probe PACu-1 shows promising potential for non-invasive assessment of hepatic Cu levels, showcasing successful detection of elevated Cu levels in disease models and successful differentiation of disease animals in blind studies.
The development of high-performance photoacoustic (PA) probes that can monitor disease biomarkers in deep tissue has the potential to replace invasive medical procedures such as a biopsy. However, such probes must be optimized for in vivo performance and exhibit an exceptional safety profile. In this study, we have developed PACu-1, a PA probe designed for biopsy-free assessment (BFA) of hepatic Cu via photoacoustic imaging. PACu-1 features a Cu(I)responsive trigger appended to an aza-BODIPY dye platform that has been optimized for ratiometric sensing. Owing to its excellent performance, we were able to detect basal levels of Cu in healthy wild-type mice as well as elevated Cu in a Wilson's disease model and in a liver metastasis model. To showcase the potential impact of PACu-1 for BFA, we conducted two blind studies in which we were able to successfully identify Wilson's disease animals from healthy control mice in each instance.
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