Article
Multidisciplinary Sciences
Dong Han, Guangming Wu, Rui Chen, Hannes C. A. Drexler, Caitlin M. MacCarthy, Kee-Pyo Kim, Kenjiro Adachi, Daniela Gerovska, Lampros Mavrommatis, Ivan Bedzhov, Marcos J. Arauzo-Bravo, Hans R. Schoeler
Summary: This study reveals the importance of Oct4 linker interface-mediated interactions in maintaining pluripotency. Mutations in the linker disrupt the balanced Oct4 interactome, leading to impaired epigenetic state and self-renewal ability of embryonic stem cells. Restoring the expression levels of related factors can rescue the pluripotency of linker mutant ESCs.
Article
Critical Care Medicine
Nathan T. P. Patel, Michaela Gaffley, Mathias J. R. Leblanc, Magan R. Lane, Lauren E. Kratky, Guillaume L. Hoareau, M. Austin Johnson, James E. Jordan, Lucas P. Neff, Timothy K. Williams
Summary: EPACC with low threshold and high threshold reduced the requirement for norepinephrine and decreased the frequency of proximal hypotension in the treatment of ischemia-reperfusion injury. EPACC-Low paradoxically increased renal perfusion despite having a mechanical resistor in the aorta proximal to the renal arteries.
Article
Biochemistry & Molecular Biology
Mathias Clarysse, Alison Accarie, Ricard Farre, Emilio Canovai, Diethard Monbaliu, Jan Gunst, Gert De Hertogh, Tim Vanuytsel, Jacques Pirenne, Laurens J. J. Ceulemans
Summary: Animal research on intestinal ischemia-reperfusion injury (IRI) is mainly done using rodent models. Previously, intraperitoneal injections with ketamine-xylazine mixtures were common, but now volatile anesthetics like isoflurane are more commonly used. However, the impact of the anesthetic method on intestinal IRI has not been studied. This study aims to analyze the different anesthetic methods and their influence on the extent of intestinal IRI in a rat model.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Liu Liu, Diptadip Dattaroy, Katherine F. Simpson, Luiz F. Barella, Yinghong Cui, Yan Xiong, Jian Jin, Gabriele M. Konig, Evi Kostenis, Jefferey C. Roman, Klaus H. Kaestner, Nicolai M. Doliba, Jurgen Wess
Summary: This study demonstrates that by activating G(q) signaling pathway, glucagon can be released to improve insulin secretion and glucose tolerance. Chronic activation of this pathway also benefits glucose metabolism in obese mice. Additionally, the activation of V1bR receptor stimulates glucagon release, highlighting its role in regulating glucagon secretion under hypoglycemic conditions.
Article
Reproductive Biology
Yao Wu, Jia-Peng He, Juan Xie, Ke-zhi Wang, Jin-Wen Kang, Asgerally T. Fazleabas, Ren-Wei Su
Summary: The study revealed that Notch1 is essential for normal decidualization in the mouse uterus only in the first pregnancy, with no impact on subsequent pregnancies. The initial pregnancy losses in Notch1-deficient mice were found to be due to impaired decidualization, with a group of genes identified that contribute to Notch1-regulated decidualization in natural pregnancy involving cell-cell and cell-matrix interactions.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Martine de Boer, Maaike te Lintel Hekkert, Jiang Chang, Bibi S. van Thiel, Leonie Martens, Maxime M. Bos, Marion G. J. de Kleijnen, Yanto Ridwan, Yanti Octavia, Elza D. van Deel, Lau A. Blonden, Renata M. C. Brandt, Sander Barnhoorn, Paula K. Bautista-Nino, Ilona Krabbendam-Peters, Rianne Wolswinkel, Banafsheh Arshi, Mohsen Ghanbari, Christian Kupatt, Leon J. de Windt, A. H. Jan Danser, Ingrid van der Pluijm, Carol Ann Remme, Monika Stoll, Joris Pothof, Anton J. M. Roks, Maryam Kavousi, Jeroen Essers, Jolanda van der Velden, Jan H. J. Hoeijmakers, Dirk J. Duncker
Summary: Heart failure has become an epidemic in an aging population, and DNA damage is believed to play a role in its development. This study found that impaired DNA repair in cardiomyocytes leads to early onset of heart failure, with increased oxidative stress, fibrosis, and apoptosis. The findings suggest that DNA damage could be a potential therapeutic target for heart failure.
Article
Gastroenterology & Hepatology
Yu Zhang, Chaohui Wang, Lexi Wu, Chenhao Bai, Kaituo Huang, Lingya Yao, Zhou Zhang, Lingna Ye, Rongbei Liu, Xiaolong Ge, Mengque Xu, Yuan Zhao, Qian Cao
Summary: The downregulation of CRL4(DCAF2) in IECs may lead to intestinal barrier dysfunction in IBD, and it can increase susceptibility to inflammation by promoting cell proliferation, inhibiting cell cycle progression, and apoptosis.
DIGESTIVE DISEASES AND SCIENCES
(2023)
Article
Cell Biology
Jun Cao, Sunil K. Verma, Elizabeth Jaworski, Stephanie Mohan, Chloe K. Nagasawa, Kempaiah Rayavara, Amanda Sooter, Sierra N. Miller, Richard J. Holcomb, Mason J. Powell, Ping Ji, Nathan D. Elrod, Eda Yildirim, Eric J. Wagner, Vsevolod Popov, Nisha J. Garg, Andrew L. Routh, Muge N. Kuyumcu-Martinez
Summary: The study uncovered a role for RBFOX2 in maintaining alternative polyadenylation signatures in myoblasts, influencing the mRNA levels and isoform expression of various genes, including those related to mitochondria and muscle contraction. Depletion of RBFOX2 negatively impacted mitochondrial health in myoblasts, particularly through disruption of APA in the Slc25a4 gene.
Review
Medicine, General & Internal
Jonathan H. Pelletier, Sriram Ramgopal, Christopher M. Horvat
Summary: Multiple studies have shown a robust parabolic relationship between arterial partial pressure of oxygen (PaO2) and risk-adjusted pediatric ICU mortality, but the exact threshold at which hyperoxemia becomes deleterious remains unclear. These findings suggest that clinicians should use supplemental oxygen therapy judiciously in critically ill children.
FRONTIERS IN MEDICINE
(2021)
Article
Critical Care Medicine
Mitra Sadeghi, Anna Stene Hurtsen, Josephine Tegenfalk, Per Skoog, Kjell Jansson, Tal M. Horer, Kristofer F. Nilsson
Summary: In a porcine model of normovolemia and hemorrhagic shock, aortic occlusion gradually lowered distal blood flow and pressure, while ETCO2, VO2, and carbon dioxide production decreased at 66% and 100% aortic occlusion. ETCO2 correlated significantly to distal aortic blood flow and systemic VO2 during both normovolemia and hemorrhage.
Article
Biochemistry & Molecular Biology
Claudia Penna, Saveria Femmino, Fabrizio Caldera, Alberto Rubin Pedrazzo, Claudio Cecone, Edoardo Alfi, Stefano Comita, Takanobu Higashiyama, Francesco Trotta, Pasquale Pagliaro, Roberta Cavalli
Summary: This study examined the efficacy of novel CNN-based oxygen nanocarriers in controlled oxygenation, showing a significant reduction in cell mortality during hypoxia/reoxygenation when compared to nitrogen-based nanocarriers. O-2-CNN demonstrated promising potential for future medical applications in myocardial infarction treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Zhao Chen, Zhecheng Wang, Deshun Liu, Xuzi Zhao, Shili Ning, Xingming Liu, Guangzhi Wang, Feng Zhang, Fuwen Luo, Jihong Yao, Xiaofeng Tian
Summary: This study found that caveolin-1 (Cav1) plays a critical role in protecting against intestinal ischemia reperfusion (I/R) injury by regulating protein kinase C beta II (PKC beta II) inactivation. Inhibition of Cav1 resulted in excessive PKC beta II activation and worsened intestinal injury. This finding offers a new therapeutic strategy for the prevention and treatment of intestinal I/R injury.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Gastroenterology & Hepatology
Janine Hafliger, Marlene Schwarzfischer, Kirstin Atrott, Claudia Stanzel, Yasser Morsy, Marcin Wawrzyniak, Silvia Lang, Tomas Valenta, Konrad Basler, Gerhard Rogler, Michael Scharl, Marianne R. Spalinger
Summary: The loss of GP96 in the adult mice intestine leads to the degeneration of the stem cell niche, the eradication of the epithelial layer, and ultimately death. This is due to defects in Wnt and Notch signaling, as well as massive induction of endoplasmic reticulum stress. GP96 also plays a critical role in regulating the renewal and differentiation of intestinal stem cells.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2023)
Article
Urology & Nephrology
Nir Polak, Gregory Fishelev, Erez Lang, Zemer Wang, Amos Neheman, Yacov Ben Haim, Amir Hadanny, Shai Efrati
Summary: Ischemic complications of glans penis following circumcision are rare but can result in irreversible necrosis with severe long-term consequences. Hyperbaric oxygen treatment (HBOT) can be utilized as salvage treatment for circumcision-induced glans ischemia as early as possible to prevent full necrosis.
Article
Behavioral Sciences
Caitlin A. Madison, Lauren Hillbrick, Jacob Kuempel, Georgia Lee Albrecht, Kerstin K. Landrock, Stephen Safe, Robert S. Chapkin, Shoshana Eitan
Summary: This study suggests that the aryl hydrocarbon receptor (AhR) in the intestine plays a crucial role in depression, anxiety, and spatial learning. AhR-active microbial metabolites can modulate neuronal responses. The antidepressant effects of DIM and DHNA are not dependent on the expression of intestinal AhR.
BEHAVIOURAL BRAIN RESEARCH
(2023)
Article
Medicine, Research & Experimental
Holger A. Lindner, Shigehiko Schamoni, Thomas Kirschning, Corinna Worm, Bianka Hahn, Franz-Simon Centner, Jochen J. Schoettler, Michael Hagmann, Joerg Krebs, Dennis Mangold, Stephanie Nitsch, Stefan Riezler, Manfred Thiel, Verena Schneider-Lindner
Summary: Background: Sepsis is a major cause of death in the ICU. Improving the speed of diagnosis can help improve survival rates. This study collected ground truth labels for sepsis to address the issue of defining sepsis. The findings showed that SIRS ranked higher than sepsis in assessing disease severity, and the accuracy of Sepsis-1/2 and Sepsis-3 in detecting sepsis onset was questioned.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Stany Sandrio, Joerg Krebs, Eva Leonardy, Manfred Thiel, Jochen J. Schoettler
Summary: This study found that the pre-ECMO VIS is an independent predictor of survival in patients with severe cardiopulmonary failure supported with ECMO, and can assist in selecting the appropriate ECMO cannulation strategy.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Franz-Simon Centner, Mariella Eliana Oster, Franz-Joseph Dally, Johannes Sauter-Servaes, Tanja Pelzer, Jochen Johannes Schoettler, Bianka Hahn, Anna-Meagan Fairley, Amr Abdulazim, Katharina Antonia Margarete Hackenberg, Christoph Groden, Nima Etminan, Joerg Krebs, Manfred Thiel, Holger Wenz, Mate Elod Maros
Summary: Data on sepsis in patients with a subarachnoid hemorrhage are limited. This study evaluated the impact of different sepsis criteria on the outcome in a cohort of SAH patients. The findings suggest that the modified Sepsis-3 criteria and alternative sepsis criteria may provide more accurate diagnosis of sepsis in SAH patients and are associated with prognosis.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Anesthesiology
Alice Marguerite Conrad, Gregor Loosen, Christoph Boesing, Manfred Thiel, Thomas Luecke, Patricia R. M. Rocco, Paolo Pelosi, Joerg Krebs
Summary: In patients with severe ARDS managed with V-V ECMO, increasing extracorporeal blood flow alters the calculation of lung water index (EVLWI) and intrathoracic blood volume index (ITBVI), resulting in unreliable measurements.
JOURNAL OF CLINICAL MONITORING AND COMPUTING
(2023)
Article
Anesthesiology
Christoph Boesing, Laura Schaefer, Marvin Hammel, Mirko Otto, Susanne Blank, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: This study compared the end-expiratory transpulmonary pressure between fixed and individualized positive end-expiratory pressure (PEEP) strategies in superobese patients and analyzed their effects on respiratory mechanics, end-expiratory lung volume, gas exchange, and hemodynamic parameters. The study found that individualized PEEP strategy resulted in lower end-expiratory transpulmonary pressure while improving respiratory mechanics, lung volume, and oxygenation. Therefore, individualized PEEP strategy may be a feasible approach for respiratory support in superobese patients undergoing laparoscopic surgery.
Article
Health Care Sciences & Services
Julia Michels, Jan Meis, Noemi Sturm, Florian Bornitz., Selina von Schumann., Aline. Weis, Benjamin. Neetz, Martina Bentner, Johanna Forstner, Nicola Litke, Michel Wensing, Stella Erdmann, Thomas Grobe, Timm Frerk, Axel Kempa, Claus Neurohr, Armin Schneider, Michael Mueller, Felix J. F. Herth, Joachim C. Szecsenyi, Franziska Trudzinski, Franziska Christina Trudzinski
Summary: This study aims to evaluate strategies for preventing long-term invasive mechanical ventilation (IMV) and improving weaning expertise and cooperation with adjacent intensive care units. Patient recruitment will be conducted in 40 intensive care units, and the primary outcome of successful weaning from IMV will be evaluated using mixed regression models.
BMC HEALTH SERVICES RESEARCH
(2023)
Article
Anesthesiology
Christoph Boesing, Laura Schaefer, Jochen Johannes Schoettler, Alena Quentin, Grietje Beck, Manfred Thiel, Patrick Honeck, Karl-Friedrich Kowalewski, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: This study compares the effects of different individualized PEEP titration strategies during Trendelenburg position with pneumoperitoneum. The results show that PEEP Delta P strategy can reduce lung stress and strain, and improve gas exchange. However, PEEP Delta P may result in decreased cardiac output.
EUROPEAN JOURNAL OF ANAESTHESIOLOGY
(2023)
Article
Medicine, General & Internal
Stany Sandrio, Manfred Thiel, Joerg Krebs
Summary: We investigated the association between pre-ECMO liver impairment, V-VA ECMO support, and mortality in patients undergoing ECMO treatment. We found that a higher pre-ECMO MELD score independently predicts mortality, and V-VA ECMO is associated with a higher mortality risk in patients with a pre-ECMO MELD score >16. These findings suggest the importance of assessing liver function and considering the type of ECMO support in predicting and managing mortality risk.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Microbiology
Jochen Johannes Schoettler, Stany Sandrio, Christoph Boesing, Lena Bauer, Thomas Miethke, Manfred Thiel, Joerg Krebs
Summary: Viral pneumonia frequently leads to bacterial co- or superinfection, which has negative effects on patients' outcomes. However, the incidence and impact of co- or superinfection might vary depending on the type of viral pneumonia. In this retrospective study, we found that the incidence of bacterial co- and superinfection did not differ significantly between COVID-19 pneumonia and influenza pneumonia patients. Furthermore, co- or superinfection was not an independent predictor of mortality in patients with high disease severity. However, there was a significantly higher probability of superinfection in COVID-19 pneumonia patients compared to influenza pneumonia patients.
Article
Critical Care Medicine
Christoph Boesing, Laura Schaefer, Peter T. Graf, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: In ARDS patients treated with VV ECMO, adjusting PEEP to 10 cmH(2)O can reduce MP, improve hemodynamic parameters, and increase oxygen delivery.
JOURNAL OF CRITICAL CARE
(2024)
Article
Medicine, Research & Experimental
Sixten Koerper, Beate Gruener, Daniel Zickler, Thomas Wiesmann, Patrick Wuchter, Rainer Blasczyk, Kai Zacharowski, Peter Spieth, Torsten Tonn, Peter Rosenberger, Gregor Paul, Jan Pilch, Joachim Schwaeble, Tamam Bakchoul, Thomas Thiele, Julian Knoerlein, Matthias M. Dollinger, Joerg Krebs, Martin Bentz, Victor M. Corman, Dzenan Kilalic, Gerlinde Schmidtke-Schrezenmeier, Philipp M. Lepper, Lucas Ernst, Hinnerk Wulf, Alexandra Ulrich, Manfred Weiss, Jan Matthias Kruse, Thomas Burkhardt, Rebecca Mueller, Harald Klueter, Michael Schmidt, Bernd Jahrsdoerfer, Ramin Lotfi, Markus Rojewski, Thomas Appl, Benjamin Mayer, Philipp Schnecko, Erhard Seifried, Hubert Schrezenmeier
Summary: Results from an extended observation of a randomized trial on COVID-19 convalescent plasma treatment showed a trend towards better outcomes in the CCP group compared to the control group, with significantly improved long-term survival seen in patients receiving higher levels of neutralizing antibodies. The study also found no significant difference in the increase of neutralizing antibodies after vaccination between the two groups.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Clinical Neurology
Amr Abdulazim, Carla Kueppers, Katharina A. M. Hackenberg, Eva Neumaier-Probst, Mohamad Mansour Alzghloul, Joerg Krebs, Manfred Thiel, Hester Lingsma, Gabriel J. E. Rinkel, Christoph Groden, Nima Etminan
Summary: The study demonstrates that implementing a multidisciplinary approach with standardized neurological and radiological assessments, as well as personalized medical treatment strategies, can significantly reduce cerebral infarction and unfavorable functional outcomes after aneurysmal subarachnoid hemorrhage.
ACTA NEUROCHIRURGICA
(2022)
Article
Critical Care Medicine
Christoph Boesing, Peter T. Graf, Fabian Schmitt, Manfred Thiel, Paolo Pelosi, Patricia R. M. Rocco, Thomas Luecke, Joerg Krebs
Summary: The study demonstrates that prone positioning combined with appropriate PEEP levels can increase transpulmonary pressures, improve oxygenation, and stabilize hemodynamics in patients with moderate to severe ARDS. Using a lower PEEP strategy (PEEPARDSNetwork) in conjunction with prone positioning may be an effective lung protective ventilation strategy for these patients.