Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 163, Issue -, Pages 317-326Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2021.04.005
Keywords
Aleurone layers; Hydrogen-rich water; Programmed cell death; Reactive oxygen species; Triticum aestivum
Categories
Funding
- National Natural Science Foundation of China, China [31701347]
- Tobacco Genome Projects of CNTC, China [110201901020 (JY-07)]
- Science and Technology Development Fund of President of Zhengzhou Tobacco Research Institute, China [902018CA0260]
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Hydrogen gas is considered a novel gaseous signaling molecule in plants, while hydrogen rich water has been shown to delay wheat aleurone layer programmed cell death induced by gibberellic acid by reducing reactive oxygen species levels.
Hydrogen gas (H-2) has been recently regarded as a novel gaseous signaling molecule that performs multiple functional roles in plant. Here, we demonstrate that hydrogen rich water (HRW)-an experimentally tractable reagent to assess the effects of the H-2 significantly delays wheat aleurone layer programmed cell death (PCD) induced by gibberellic acid (GA). Endogenous H-2 production exhibited lower level in aleurone layers under GA treatment, whereas the H-2 production was apparently increased under abscisic acid (ABA) treatment. HRW not only increased H-2 production but also delayed GA-induced PCD. We further observed that application of HRW substantially prevented the increases of hydrogen peroxide (H2O2) and superoxide anion radical (O-2(center dot-)) triggered by GA. HRW also directly react with hydroxyl radical (center dot OH) to delay GA-induced PCD. Quantitative real-time PCR (qRT-PCR) and biochemical assays showed that HRW induced the transcripts and enzymatic activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) that metabolize reactive oxygen species (ROS); these increases coincided with the observed changes in O-2(center dot-), H2O2 and center dot OH accumulation upon GA treatment. Our study therefore suggests that HRW-triggered alleviation of wheat aleurone layer PCD induced by GA results from a combination of H-2-mediated decreases of ROS levels, including O-2(center dot-), H2O2, and center dot OH.
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