Article
Plant Sciences
Edgar Perez-Matas, Pascual Garcia-Perez, Begona Miras-Moreno, Luigi Lucini, Mercedes Bonfill, Javier Palazon, Diego Hidalgo-Martinez
Summary: This study investigates the effects of coronatine (COR) and salicylic acid (SA) elicitors on taxane-associated metabolism in Taxus baccata cell cultures. Untargeted metabolomics analysis reveals pleiotropic effects induced by COR and SA, leading to changes in cell growth, taxane content, and secondary metabolism. Metabolite annotation shows significant effects on N-containing compounds, phenylpropanoids, and terpenoids. The highest level of paclitaxel content is observed on day 8 under SA elicitation, negatively correlating with the biomarkers kauralexin A2 and taxusin. This study provides valuable insights into the intricate metabolic changes associated with paclitaxel production.
Article
Plant Sciences
Edgar Perez-Matas, Abdulsamie Hanano, Elisabeth Moyano, Mercedes Bonfill, Rosa M. Cusido, Javier Palazon
Summary: This study found that using COR and beta-CDs can enhance the production and release of paclitaxel in Taxus baccata cell suspensions. Furthermore, the role of lipid droplets in the trafficking and accumulation of paclitaxel in Taxus spp. cell cultures was investigated, and it was found that lipid droplets can store and distribute paclitaxel.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ainoa Escrich, Diego Hidalgo, Mercedes Bonfill, Javier Palazon, Raul Sanchez-Munoz, Elisabeth Moyano
Summary: Novel approaches to optimize the production of plant specialized metabolites are crucial to reach maximum productivity of plant biofactories. Plant polyploidization frequently enhances protein synthesis and thereby increases the biosynthesis of specialized metabolites. By treating immature embryos of Taxus baccata with colchicine, polyploid cell lines were successfully induced, which showed higher taxane pathway gene expression and 6.2-fold higher taxane production compared to diploid cells. This finding is a step forward towards developing a sustainable production system for the anticancer drug paclitaxel.
Article
Cell Biology
Katarzyna Syklowska-Baranek, Grazyna Sygitowicz, Agata Maciejak-Jastrzebska, Agnieszka Pietrosiuk, Anna Szakiel
Summary: By priming with BABA and eliciting with MIX, the paclitaxel yield and expression levels of related genes can be significantly increased.
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biochemistry & Molecular Biology
Edgar Perez-Matas, Pascual Garcia-Perez, Mercedes Bonfill, Luigi Lucini, Diego Hidalgo-Martinez, Javier Palazon
Summary: Elicited cell cultures of Taxus spp. are sustainable biotechnological production systems of the anticancer drug paclitaxel. This study investigates the impact of elicitation on the synthesis of other bioactive compounds and reveals the differential effects of elicitors on cell growth and phenolic biosynthesis in Taxus baccata cell suspensions. Untargeted metabolomics analysis identifies numerous phenolic compounds and their changes over time, indicating the potential of Taxus ssp. biofactories to supply taxanes and valuable phenolic antioxidants.
Article
Plant Sciences
Xingyao Xiong, Junbo Gou, Qinggang Liao, Yanlin Li, Qian Zhou, Guiqi Bi, Chong Li, Ran Du, Xiaotong Wang, Tianshu Sun, Lvjun Guo, Haifei Liang, Pengjun Lu, Yaoyao Wu, Zhonghua Zhang, Dae-Kyun Ro, Yi Shang, Sanwen Huang, Jianbin Yan
Summary: This study completed the chromosome-level genome sequencing of Taxus, revealing its genome evolution and the biosynthesis pathways of paclitaxel and taxadiene. A unique physical and functional grouping of CYP725As for paclitaxel biosynthesis was discovered in the Taxus genome, along with a gene cluster mainly formed by gene duplications for taxadiene biosynthesis.
Article
Biochemistry & Molecular Biology
Michelle C. McKee, Sarah A. Wilson, Susan C. Roberts
Summary: Research has shown that methyl jasmonate induction can increase paclitaxel accumulation, but inhibits cell growth and increases phenylpropanoid biosynthesis. The study revealed that the impact of methyl jasmonate varies across generations of cells, and that paclitaxel accumulation is indirectly correlated with aggregate size.
Article
Cell & Tissue Engineering
Carlos A. Pinzon-Arteaga, Yinjuan Wang, Yulei Wei, Ana E. Ribeiro Orsi, Leijie Li, Giovanna Scatolin, Lizhong Liu, Masahiro Sakurai, Jianfeng Ye, Hao Ming, Leqian Yu, Bo Li, Zongliang Jiang, Jun Wu
Summary: We developed an efficient method to generate bovine blastocyst-like structures (blastoids) by assembling bovine trophoblast stem cells and expanded potential stem cells. Bovine blastoids resemble blastocysts in morphology, cell composition, single-cell transcriptomes, in vitro growth, and the ability to elicit maternal recognition of pregnancy following transfer to recipient cows. Bovine blastoids serve as an accessible in vitro model for studying embryogenesis and improving reproductive efficiency in livestock species.
Article
Plant Sciences
Ana Belen Sabater-Jara, Maria Jesus Marin-Marin, Lorena Almagro, Maria Angeles Pedreno
Summary: In this study, the effect of different elicitors and their combined treatments on triterpene compound production and the expression of key biosynthetic genes in Solanum lycopersicum cv. Micro-Tom suspension-cultured cells were analyzed. The results showed that β-cyclodextrins (CD) act as true elicitors of triterpene biosynthesis and can promote the release of bioactive compounds from tomato cells into the extracellular medium.
Article
Biochemistry & Molecular Biology
Michela Alfieri, Iride Mascheretti, Romeo A. Dougue Kentsop, Roberto Consonni, Franca Locatelli, Monica Mattana, Gianluca Ottolina
Summary: In this study, adventitious root cultures of three Linum species were developed to produce aryltetralin lignans, with L. dolomiticum showing the highest productivity for various compounds such as Podophyllotoxin. Elicitors methyl jasmonate and coronatine had different effects on the production of specific lignans, indicating potential applications in antiviral and antineoplastic research.
Article
Agricultural Engineering
Setareh Golinejad, Mohammad Hossein Mirjalili, Hassan Rezadoost, Mansour Ghorbanpour
Summary: This study investigated the effects of gold nanoparticles on cell culture of Taxus baccata and found that gold nanoparticles can change the morphology and biochemical traits of the cells and increase the production of taxanes, especially taxol.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biotechnology & Applied Microbiology
Bekir Ahmet Ilgar, Sibel Ozden, Fatma Elif Cepni Yuzbasioglu, Neslihan Turgut Kara
Summary: Yeast extract was found to increase diosgenin levels in fenugreek cell suspension cultures. Additionally, it led to increased expression of the CYP86A2 gene and diosgenin content. The study also explored changes in the expression of key enzyme genes involved in diosgenin formation.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biochemistry & Molecular Biology
Laura Ravazzolo, Benedetto Ruperti, Marco Frigo, Oriana Bertaiola, Giovanna Pressi, Mario Malagoli, Silvia Quaggiotti
Summary: Purple coneflower is an economically important medicinal plant with various industrial applications. By optimizing the production of chicoric acid in cell culture, methyl jasmonate was found to be an effective elicitor to enhance the content of chicoric acid. Potential molecular markers were identified for the early prediction of chicoric acid content.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hak-Gyun Kim, Jin-Hyun Kim
Summary: This study analyzed the kinetics and mechanism of ultrasonic extraction of paclitaxel from Taxus chinensis using ultrasonic cavitation bubbles and gas bubbles. The results showed higher yields, extraction rate constants, effective diffusion coefficients, and mass transfer coefficients in ultrasonic extraction compared to extraction using gas bubbles. The investigation highlighted the key role of cavitation bubbles in promoting cell disruption and improving the recovery efficiency of paclitaxel.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Lorena Almagro, Antonio A. Calderon, Maria A. Pedreno, Maria A. Ferrer
Summary: Salt treatments significantly alter the production of bioactive phenolic compounds in grapevine suspension cultured cells. Cellular redox homeostasis is maintained by increasing the accumulation of phenolic compounds. The antioxidative network shows a differential response to the presence of elicitors under saline conditions.
Review
Biotechnology & Applied Microbiology
Sarah Otun, Ainoa Escrich, Ikechukwu Achilonu, Molemi Rauwane, Jordy Alexis, Lerma-Escalera, Jose Ruben Morones-Ramirez, Leonardo Rios-Solis
Summary: Biotechnological approaches have significantly improved the potential of cassava crops by addressing key constraints, but the development and acceptance of improved cultivars remain challenging and require holistic solutions.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Edgar Perez-Matas, Abdulsamie Hanano, Elisabeth Moyano, Mercedes Bonfill, Rosa M. Cusido, Javier Palazon
Summary: This study found that using COR and beta-CDs can enhance the production and release of paclitaxel in Taxus baccata cell suspensions. Furthermore, the role of lipid droplets in the trafficking and accumulation of paclitaxel in Taxus spp. cell cultures was investigated, and it was found that lipid droplets can store and distribute paclitaxel.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Ainoa Escrich, Rosa M. Cusido, Mercedes Bonfill, Javier Palazon, Raul Sanchez-Munoz, Elisabeth Moyano
Summary: Environmental conditions play a key role in regulating gene expression in plants through epigenetic mechanisms, including DNA methylation. This study focuses on the methylation patterns of genes involved in taxane biosynthesis in Taxus x media cell cultures and reveals the existence of specific epigenetic regulatory mechanisms affecting taxane production. The findings suggest that the methylation levels of different gene promoters vary, and the regulatory function of transcription factors may be compromised in old cell cultures, leading to reduced taxane production.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Dalia Abdel-Fattah H. Selim, Muhammad Zayed, Maha M. E. Ali, Heba S. Eldesouky, Mercedes Bonfill, Amira M. El-Tahan, Omar M. Ibrahim, Mohamed T. El-Saadony, Khaled A. El-Tarabily, Synan F. AbuQamar, Samira Elokkiah
Summary: This study assessed the impact of irrigation with magnetically treated seawater on wheat. The results showed that magnetic treatment of seawater had beneficial effects on plant growth, water relations, biochemical characteristics, and yield components. The germination rate and yield of wheat increased significantly when irrigated with magnetic seawater.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Miguel Angel Alcalde, Edgar Perez-Matas, Ainoa Escrich, Rosa M. Cusido, Javier Palazon, Mercedes Bonfill
Summary: The aim of plant in vitro culture is to produce secondary plant metabolites, and root culture is an important biotechnological system for this purpose. Elicitors have been used to increase production by activating biosynthetic pathways. This review analyzes the application of biotic elicitors in adventitious and hairy root cultures from 2010 to 2022.
Article
Plant Sciences
Miguel Angel Alcalde, Maren Muller, Sergi Munne-Bosch, Mariana Landin, Pedro Pablo Gallego, Mercedes Bonfill, Javier Palazon, Diego Hidalgo-Martinez
Summary: Through the study of C. asiatica hairy root cultures, we established the links between the expressions of rol and aux genes and morphological traits, hormone profile, and centelloside production. We found that abscisic acid (ABA) and cytokinin isopentenyl adenosine (IPA) play critical roles in defining the morphological traits and centelloside content.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Edgar Perez-Matas, Diego Hidalgo-Martinez, Ainoa Escrich, Miguel Angel Alcalde, Elisabeth Moyano, Mercedes Bonfill, Javier Palazon
Summary: Paclitaxel and its derivatives are widely used chemotherapeutic agents for cancer treatment, but their production remains challenging due to scarcity. This review discusses the progress in improving taxane production through genetic engineering techniques, including the overexpression of limiting genes and transcription factors in Taxus spp. cells and transformed roots, as well as attempts to produce taxanes in heterologous organisms. However, the transfer of the complete PTX metabolic route has not been achieved yet, and taxane biosynthesis is still restricted to Taxus cells and some endophytic fungi.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Ainoa Escrich, Diego Hidalgo, Mercedes Bonfill, Javier Palazon, Raul Sanchez-Munoz, Elisabeth Moyano
Summary: Novel approaches to optimize the production of plant specialized metabolites are crucial to reach maximum productivity of plant biofactories. Plant polyploidization frequently enhances protein synthesis and thereby increases the biosynthesis of specialized metabolites. By treating immature embryos of Taxus baccata with colchicine, polyploid cell lines were successfully induced, which showed higher taxane pathway gene expression and 6.2-fold higher taxane production compared to diploid cells. This finding is a step forward towards developing a sustainable production system for the anticancer drug paclitaxel.
Article
Biochemistry & Molecular Biology
Edgar Perez-Matas, Pascual Garcia-Perez, Mercedes Bonfill, Luigi Lucini, Diego Hidalgo-Martinez, Javier Palazon
Summary: Elicited cell cultures of Taxus spp. are sustainable biotechnological production systems of the anticancer drug paclitaxel. This study investigates the impact of elicitation on the synthesis of other bioactive compounds and reveals the differential effects of elicitors on cell growth and phenolic biosynthesis in Taxus baccata cell suspensions. Untargeted metabolomics analysis identifies numerous phenolic compounds and their changes over time, indicating the potential of Taxus ssp. biofactories to supply taxanes and valuable phenolic antioxidants.
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Plant Sciences
Edgar Perez-Matas, Pascual Garcia-Perez, Begona Miras-Moreno, Luigi Lucini, Mercedes Bonfill, Javier Palazon, Diego Hidalgo-Martinez
Summary: This study investigates the effects of coronatine (COR) and salicylic acid (SA) elicitors on taxane-associated metabolism in Taxus baccata cell cultures. Untargeted metabolomics analysis reveals pleiotropic effects induced by COR and SA, leading to changes in cell growth, taxane content, and secondary metabolism. Metabolite annotation shows significant effects on N-containing compounds, phenylpropanoids, and terpenoids. The highest level of paclitaxel content is observed on day 8 under SA elicitation, negatively correlating with the biomarkers kauralexin A2 and taxusin. This study provides valuable insights into the intricate metabolic changes associated with paclitaxel production.
Article
Plant Sciences
Miguel Angel Alcalde, Javier Palazon, Mercedes Bonfill, Diego Hidalgo-Martinez
Summary: This study explored the effects of overexpressing the squalene synthase (SQS) gene and the transcription factor TSAR2 on various aspects of C. asiatica hairy root lines. The transformed lines showed noticeable alterations in morphological traits and content of centelloside, phytosterol, and squalene. The expression of T-DNA genes modulated the centelloside biosynthesis genes. These findings provide valuable information for the biotechnological application of C. asiatica hairy roots.
Article
Plant Sciences
Wanli You, Jinglin Zhang, Xueyin Ru, Feng Xu, Zhengguo Wu, Peng Jin, Yonghua Zheng, Shifeng Cao
Summary: This study investigated the effect of calcium chloride (CaCl2) treatment on GABA accumulation in fresh-cut cantaloupe and the underlying mechanisms. The results showed that CaCl2 treatment increased GABA content and the activities of GAD and SSADH enzymes, while reducing glutamate content and GABA-T activity. Additionally, CaCl2 treatment upregulated the expressions of CmCML11 and CmCAMTA5, as well as several GABA shunt genes, through the transcriptional activation by CmCAMTA5. Furthermore, the interaction between CmCML11 and CmCAMTA5 enhanced the transcriptional activation of GABA shunt genes. Overall, this study reveals that CaCl2 treatment promotes GABA accumulation in fresh-cut cantaloupe through the combined effect of CmCML11 and CmCAMTA5 in regulating the expressions of GABA shunt genes.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Min Wang, Yupeng Wang, Xiaohui Wang, Guangwei Wei, Huiyi Yang, Xi Yang, Tinghai Shen, Huijie Qu, Sheng Fang, Ziming Wu
Summary: This study identified the high nitrogen use efficiency (NUE) black sesame variety 17-156 and analyzed its underlying physiological and molecular mechanisms. The results showed that 17-156 possesses a sophisticated nitrogen metabolizing machinery to uptake and assimilate higher quantities of inorganic nitrogen, simultaneously improving carbon metabolism and growth. Many important genes were up-regulated in 17-156 under high nitrogen condition. Additionally, 38 potential candidate genes were identified for future studies to improve sesame's NUE. These findings provide valuable resources for understanding the regulatory network of nitrogen metabolism and developing sesame cultivars with improved NUE.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
He Dong, Chongmei Xu, Chengtao Zhang, Li Zhang, Yaqin Yao, Suiqi Zhang
Summary: The study found that short cells in maize leaves not only improve leaf mechanical support and photosynthetic performance, enhance drought resistance, but also participate in stomatal regulation.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Na Liu, Wenyan Shang, Mengxin Guan, Jibin Xiao, Guangxiang Tian, Baozhan Ma, Wenjing Shang, Xu Li, Shijia Zhao, Chuang Li, Kun Cheng, Wenming Zheng
Summary: This study cloned the full-length cDNA sequence of TaSPX3 gene in wheat and found that TaSPX3 responds to low phosphorus stress in multiple wheat genotypes. Overexpressing TaSPX3 can alleviate phosphorus deficiency symptoms and promote plant growth in Arabidopsis. The study also revealed the interaction of TaSPX3 with other genes related to the phosphorus starvation signaling pathway.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Kongyuan Wu, Lizhen Wang, Zihan Wu, Ziqing Liu, Zengfei Li, Jun Shen, Shengjie Shi, Hong Liu, Christopher Rensing, Renwei Feng
Summary: Selenium (Se) can reduce uptake and translocation of cadmium (Cd) in plants by regulating root morphology. This study investigated the effects of Se(IV) on root exudates, root morphology, root endogenous hormones, and Cd uptake efficiency in rice under Cd stress. The results showed that Se(IV) significantly reduced Cd concentrations in shoots and roots, and decreased Cd uptake efficiency via root hairs. Se(IV) also affected root morphology, root exudates, and the synthesis of hormones like IAA and JA. However, transcriptome analysis revealed no upregulated differentially expressed genes (DEGs) in IAA synthesis.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)
Article
Plant Sciences
Di Wang, Huaifang Zhang, Xuefei Hu, Haizhen Zhang, Shuang Feng, Aimin Zhou
Summary: This study identified a cell number regulator gene called SlCNR8 in willow, which enhances resistance to trace metals in transgenic poplar seedlings. SlCNR8 reduces Cd uptake and accumulation, and can be used as a candidate gene for genetic improvement of phytostabilisation of trace metals.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2024)