Article
Biotechnology & Applied Microbiology
Yuanyuan Shi, Yingxin Wang, Zhaochen Shan, Zhenhua Gao
Summary: Decellularized submandibular gland extracellular matrix (DSMG) was found to have similar structure and composition to dental pulp. DSMG could serve as an alternative scaffold for dental pulp regeneration, supporting adhesion and proliferation of dental pulp stem cells. Implanted cell-seeded DSMG formed a vascularized dental pulp-like tissue expressing markers involved in dentinogenesis and angiogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Chemistry, Physical
Necdet Adanir, Zohaib Khurshid, Jithendra Ratnayake
Summary: This systematic review evaluates the effectiveness of decellularized dental pulp extracellular matrix (DP-ECM) in pulpal regeneration. Although the results of the studies suggest that DP-ECM may stimulate pulpal regeneration, the presence of bias and methodological deficiencies limit the ability to draw definitive conclusions. Further research, both clinical and pre-clinical, is needed to provide more conclusive evidence.
Article
Biochemistry & Molecular Biology
Francesca Diomede, Luigia Fonticoli, Guya Diletta Marconi, Ylenia Della Rocca, Thangavelu Soundara Rajan, Oriana Trubiani, Giovanna Murmura, Jacopo Pizzicannella
Summary: The study aimed to develop an in vitro culture system using decellularized dental pulp (DDP) matrix, 5-Aza-2'-deoxycytidine (5-Aza), and extracellular vesicles (EVs) derived from human dental pulp stem cells (hDPSCs) to facilitate dental pulp regeneration.
Article
Biotechnology & Applied Microbiology
Jiahui Fu, Jianfeng Chen, Wenjun Li, Xiaomin Yang, Jingyan Yang, Huixin Quan, Haitao Huang, Gang Chen
Summary: The supplementation of laminins to DPEM enhances odontogenic differentiation of cells and contributes to the formation of the odontoblast layer in dental pulp regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Liwen Zheng, Yaxian Liu, Lin Jiang, Xiaoping Wang, Yuqin Chen, Lan Li, Mingyu Song, Hongmei Zhang, Yu Shrike Zhang, Ximu Zhang
Summary: This study proposes a new strategy for pulp regeneration by using hydrogel microspheres incorporated with decellularized dental pulp matrix-derived bioactive factors to simulate a pulp-specific three-dimensional microenvironment. The constructed microspheres exhibit favorable plasticity, biocompatibility, and biological performances, and hDPSCs cultured on the microspheres demonstrate enhanced pulp-formation ability. In vivo experiments show that the microspheres facilitate the regeneration of pulp-like tissue and new dentin. The decellularized pulp matrix-derived bioactive factors mediate the multi-directional differentiation of hDPSCs to regenerate the pulp tissue.
ACTA BIOMATERIALIA
(2023)
Article
Dentistry, Oral Surgery & Medicine
H. Alghutaimel, X. Yang, B. Drummond, H. Nazzal, M. Duggal, E. Raif
Summary: The study focused on investigating the vascularization capacity of a decellularized dental pulp matrix seeded with human dental pulp stem cells both in vitro and in vivo. Findings showed that recellularization with hDPSCs led to enhanced vascularization and expression of angiogenesis markers, with significant improvement in vascularization compared to unseeded groups. This suggests a promising strategy for dental pulp regeneration with the combination of DDP and hDPSCs.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Chao Liang, Li Liao, Weidong Tian
Summary: Decellularized extracellular matrix (dECM) promotes stem cell proliferation, migration, adhesion, and differentiation, making it a promising biomaterial for periodontal tissue engineering. It effectively preserves ECM components as they are in native tissue, providing ideal cues for regeneration and repair. Different sources of dECM have different advantages in promoting periodontal tissue regeneration. Various methods have been developed to improve the mechanical strength of dECM, such as functionalized scaffolds and crosslinked soluble dECM. dECM has been successful in periodontal regeneration therapies and holds potential for future applications.
Article
Engineering, Biomedical
Akihiro Nishiguchi, Tetsushi Taguchi
Summary: In this study, dECM-based tissue adhesives using a pH-driven genipin-gelator were developed. The hydrogels demonstrated high biocompatibility and tissue adhesive properties, making them useful for sealing wounds and preventing postoperative complications.
ACTA BIOMATERIALIA
(2021)
Review
Medicine, Research & Experimental
Huihui Zhang, Yilin Wang, Zijun Zheng, Xuerong Wei, Lianglong Chen, Yaobin Wu, Wenhua Huang, Lei Yang
Summary: 3D bioprinting technology can replicate native tissue and organ microenvironments by precisely placing cells in bioinks. However, finding the ideal bioink is a challenge. Organ-derived decellularized ECM (dECM) bioink has optimal biomimetic properties but is non-printable due to poor mechanical properties. Recent studies have focused on improving the printability of dECM bioinks. This review highlights the methods used to produce these bioinks, strategies to improve printability, and advancements in tissue regeneration. Challenges and potential applications are also discussed.
Article
Pathology
Harriet Ravenscroft, Ikhlas El Karim, Anna D. Krasnodembskaya, Brendan Gilmore, Imad About, Fionnuala T. Lundy
Summary: The study revealed that the secretome of dental pulp multipotent mesenchymal stromal cells (MSCs) possesses direct antibacterial activity against various bacteria, including oral pathogens. Cytokines and growth factors present in the secretome were found to contribute to this novel antibacterial activity. This suggests that human dental pulp MSCs play a role in the antibacterial properties of the dental pulp, providing new evidence for the ability of the dental pulp to fight infection and restore functional competence.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Review
Neurosciences
Maryam Mahdian, Tayebeh Sadat Tabatabai, Zahra Abpeikar, Leila Rezakhani, Mozafar Khazaei
Summary: Decellularization of organs and tissues serves a critical role in tissue engineering for the repair of neurodegenerative diseases. Decellularized scaffolds provide a suitable three-dimensional substrate for the growth of repaired nerves towards target organs, due to their lack of immunogenicity, preservation of essential factors, and high angiogenic ability.
FRONTIERS IN NEUROSCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Rong Luo, Ruonan Hu, Jiawei Xu, Peiyun Yu, Xinyu Wu, Man Zhe, Ming Liu, Fei Xing, Zhou Xiang, Changchun Zhou, Yujiang Fan, Xingdong Zhang
Summary: Decellularized extracellular matrix (dECM), as an ideal scaffold material, has been extensively studied for repairing musculoskeletal tissues due to its good biocompatibility and ability to mimic extracellular matrix microenvironment. The physicochemical properties of dECM can be enhanced by changing decellularization and modification techniques.
NANOTECHNOLOGY REVIEWS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Renan Dal-Fabbro, W. Benton Swanson, Leticia C. Capalbo, Hajime Sasaki, Marco C. Bottino
Summary: The current standard for treating irreversibly damaged dental pulp is root canal therapy, but a regenerative approach using tissue engineering and immunomodulatory biomaterials shows promise for better outcomes. This paper provides an overview of the inflammatory process in dental pulp, periapical and periodontal tissue, and discusses recent advances in treating oral diseases using biocompatible materials with immunomodulatory properties. The development of biomaterials that can modulate cells in the dental pulp complex holds significant clinical promise for improving standards of care compared to root canal therapy.
Article
Chemistry, Multidisciplinary
Xueting Yang, Yue Ma, Xiuting Wang, Shengmeng Yuan, Fangjun Huo, Genzheng Yi, Jingyi Zhang, Bo Yang, Weidong Tian
Summary: This study successfully designed a 3D bioprinted biomimetic periodontal module using a specific cell-laden bioink, which showed favorable mechanical properties and orientation guidance. The module also provided a conducive biochemical environment for regulating cell behavior. The experiments conducted on animals demonstrated that the bioprinted module significantly enhanced the regeneration of periodontal tissues and opened up new possibilities for future clinical practice.
Review
Neurosciences
Wenwei Jiang, Xuanxuan Zhang, Shumin Yu, Fangsu Yan, Jiaxi Chen, Jinyi Liu, Chuanming Dong
Summary: Functional limitation caused by spinal cord injury (SCI) imposes significant clinical and economic burden. Regenerative biomaterials can create an optimal microenvironment at the site of SCI, promoting neurogenesis, axonal development, and angiogenesis. Decellularized extracellular matrix (dECM) serves as a suitable scaffold for tissue engineering due to its good biocompatibility. However, there are still challenges to be addressed, such as obtaining materials for large-scale use, standardized production mode, the impact of decellularization on the properties of dECM, and the synergistic effect of dECM and cells.
EXPERIMENTAL NEUROLOGY
(2023)
