Journal
NEUROLOGICAL RESEARCH
Volume 44, Issue 1, Pages 24-37Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/01616412.2021.1952512
Keywords
TRPM7 channels; TRPM8 channels; TRPV1 channels; Parkinson's disease; SH-SY5Y neuronal cells
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Funding
- Scientific Research Projects Coordination Unit [SDU-BAP] [TDK-20197449]
- Coordination Unit of Academic Stuff Development Program [SDU-OYP] Suleyman Demirel University, Isparta, Turkey [OYP06164-DR-16]
- Scientific and Technological Research Council of Turkey (TUBITAK) 2211-A National Scholarship Program
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This study investigated the expression of TRPM7, TRPM8 and TRPV1 channels in SH-SY5Y cells and their role in differentiation and Parkinson's disease model induced by MPP. The findings suggest that these channels play crucial roles in cell differentiation and in the cellular response to MPP-induced Parkinson's disease model in SH-SY5Y cells.
Transient Receptor Potential Melastatin-like 7 (TRPM7), Transient Receptor Potential Melastatin-like 8 (TRPM8) and Transient Receptor Potential Vanilloid-like 1 (TRPV1) channels are expressed in neurological tissues such as brain cortex, dorsal root ganglion and hippocampal neurons and involved in several neurological diseases. The SH-SY5Y neuronal cell line is frequently used as a cellular model of neurodegenerative diseases including Parkinson's disease. The differentiated SH-SY5Y cells have much neuronal structure, function and exaggerated neuronal marker expression. However, we have less data about how differentiation induces TRP channel expression and how TRP channels have a role in cellular functions in Parkinson's disease model in SH-SY5Y cells. Hence, we aimed to investigate the effects of differentiation phenomena on TRPM7, TRPM8 and TRPV1 cation channel expression and related Ca2+ signaling. We also made some other analysis to elucidate TRP channels' function in MPP induced apoptosis, mitochondrial membrane potential levels, intracellular reactive oxygen species production, caspase 3 and caspase 9 enzyme activities in differentiated or undifferentiated SH-SY5Y neuronal cells. Herein we concluded that TRPM7, TRPM8 and TRPV1 cation channels have pivotal effects on differentiation and MPP induced Parkinson's disease model in SH-SY5Y cells.
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