Best practices and tools for reporting reproducible fluorescence microscopy methods

Comprehensive guidelines and resources to enable accurate reporting for the most common fluorescence light microscopy modalities are reported with the goal of improving microscopy reporting, rigor and reproducibility. Although fluorescence microscopy is ubiquitous in biomedical research, microscopy methods reporting is inconsistent and perhaps undervalued. We emphasize the importance of appropriate microscopy methods reporting and seek to educate researchers about how microscopy metadata impact data interpretation. We provide comprehensive guidelines and resources to enable accurate reporting for the most common fluorescence light microscopy modalities. We aim to improve microscopy reporting, thus improving the quality, rigor and reproducibility of image-based science.

Automated summary

This article provides comprehensive guidelines and resources to improve accurate reporting for the most common fluorescence light microscopy modalities, with the aim of enhancing the reporting, rigor, and reproducibility of microscopy techniques. The importance of appropriate microscopy methods reporting is emphasized, and efforts are made to educate researchers on how microscopy metadata influence data interpretation. The ultimate goal is to enhance the quality, rigor, and reproducibility of image-based science through improved microscopy reporting.