Article
Biochemistry & Molecular Biology
Yuhua Duan, Mengjun Li, Sishang Zhang, Yidan Wang, Jieya Deng, Qin Wang, Tian Yi, Xingxing Dong, Shuiyuan Cheng, Yi He, Chao Gao, Zhangqian Wang
Summary: This study discovered a strain of Bacillus subtilis T5 that can produce high yields of selenium polysaccharides and selenium nanoparticles. The strain showed tolerance to acidic pH and bile salts, high aggregation, negative hemolytic, and superior antioxidant activity, making it a potential candidate for the selenium biopharmaceuticals industry. The biosynthesized selenium nanoparticles exhibited high stability with small particle sizes. Furthermore, the strain showed enhanced alpha-amylase activity with selenite treatment, demonstrating its potential in the biopharmaceutical industry.
Article
Biology
Abdel-Moneim Eid Abdel-Moneim, Mohamed T. El-Saadony, Abdelrazeq M. Shehata, Ahmed M. Saad, Sami Ali Aldhumri, Sahar M. Ouda, Noura M. Mesalam
Summary: This study investigated the antimicrobial and antioxidant activity of Spirulina extracts and biological selenium nanoparticles. The results showed that Spirulina extracts exhibited antimicrobial and antioxidant activity, with the methanolic extract showing the highest activity. The selenium nanoparticles synthesized by Bacillus subtilis AL43 also showed antimicrobial and antioxidant effects. Spirulina and selenium nanoparticles have potential for safe and reliable medical applications.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xinchi Shang, Peng Yu, Yuwei Yin, Yue Zhang, Yuting Lu, Qiaohong Mao, Yuehong Li
Summary: The study investigated the impact of Se-rich B. subtilis on Hg-induced toxicity in fish. Results demonstrated that Se-rich B. subtilis protected the intestines from Hg-induced damage and exhibited significant antioxidant effects.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2021)
Article
Food Science & Technology
Wenqiang Shen, Yaojun Liu, Xinyue Zhang, Xiong Zhang, Xiaoping Rong, Lihong Zhao, Cheng Ji, Yuanpei Lei, Fengjuan Li, Jing Chen, Qiugang Ma
Summary: In this study, the potential ameliorative effects of probiotic Bacillus subtilis and biodegradable Bacillus subtilis on ZEN toxicosis in gilts were compared. The results showed that the biodegradable Bacillus subtilis had significant protective effects on growth performance, immune function, hormone secretion, and ZEN elimination in gilts compared to the probiotic Bacillus subtilis.
Article
Materials Science, Multidisciplinary
Badreah A. Al Jahdaly, Najlaa S. Al-Radadi, Ghada M. G. Eldin, Albandary Almahri, M. K. Ahmed, Kamel Shoueir, Izabela Janowska
Summary: Selenium nanoparticles fabricated using a green microwave technique show excellent antioxidant and degradation properties, efficiently removing dyes from aqueous solutions and exhibiting strong antibacterial activity. However, the cytotoxicity on human fibroblasts cell lines is a concern.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Biotechnology & Applied Microbiology
Shraddha Pandey, Nikee Awasthee, Anusmita Shekher, Lal Chand Rai, Subash Chandra Gupta, Santosh Kumar Dubey
Summary: The study demonstrated successful extracellular biosynthesis of SeNPs using cyanobacterium Anabaena sp. PCC 7120, showcasing potential applications in medicine and environmental bioremediation.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Yidan Wang, Yonghe Yu, Yuhua Duan, Qin Wang, Xin Cong, Yi He, Chao Gao, Muhammad Hafeez, Saad Jan, Syed Majid Rasheed, Shuiyuan Cheng, Zhangqian Wang
Summary: Selenium nanoparticles have higher bioactivity and biosafety properties. Bacillus paralicheniformis Y4 exhibits selenite tolerance and the ability to produce stable selenium nanoparticles while enhancing carboxymethyl cellulase activity. This study suggests the potential of using B. paralicheniformis Y4 for ecological removal of selenite and production of selenium nanoparticles as feed additives.
Article
Biotechnology & Applied Microbiology
Pei Liu, Haiyu Long, Han Cheng, Mengdi Liang, Zhengwei Liu, Zhenlian Han, Zhen Guo, Hao Shi, Min Sun, Shuai He
Summary: In this study, the bacterium Bacillus paramycoides 24522 was used to synthesize selenium nanoparticles. The growth conditions and efficiency of sodium selenite reduction were optimized, resulting in the production of stable red-colored selenium nanoparticles with high antibacterial and antioxidant capabilities. These findings suggest the potential applications of selenium nanoparticles in addressing selenium pollution and in the fields of environment and biotechnology.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Nutrition & Dietetics
Linbao Ji, Lian Zhang, Hu Liu, Jiakun Shen, Yu Zhang, Lin Lu, Xiujun Zhang, Xi Ma
Summary: B.subtilis M6 shows probiotic potential in improving intestinal health through altering gut microbiota.
FRONTIERS IN NUTRITION
(2022)
Article
Chemistry, Multidisciplinary
Jesus Zamora-Pineda, Olga Kalinina, Barbara A. Osborne, Katherine L. Knight
Summary: This study summarizes the active molecules from probiotic bacteria that affect immunity and have health benefits, with a particular focus on the probiotic Bacillus subtilis and its active molecule, exopolysaccharide (ESPBs).
APPLIED SCIENCES-BASEL
(2022)
Article
Microbiology
Laura M. Brutscher, Claudia Borgmeier, Sean M. Garvey, Jessica L. Spears
Summary: In this study, the safety of Bacillus subtilis BS50 was assessed through preclinical analyses. The results showed that BS50 genome does not encode any known toxins, is susceptible to commonly prescribed antibiotics, and does not have any negative effects on the viability and permeability of human intestinal epithelial cells. Therefore, BS50 has a preclinical safety profile similar to commercial probiotic strains and poses little risk to human health.
Article
Food Science & Technology
Lin Ya Eunis Keong, Mingzhan Toh, Yuyun Lu, Shao-Quan Liu
Summary: This study explored the feasibility of using probiotic Bacillus spp. for solid-state fermentation of soybean residue (okara) to modify its nutritional value and flavor property. The results showed that Bacillus subtilis R0179 significantly converted okara insoluble dietary fiber into soluble dietary fiber and increased the amounts of sugars and amino acids in okara. The fermented okara by Bacillus subtilis R0179 also had a characteristic cheesy flavor. Overall, this study suggests that probiotic bacilli can transform okara with enhanced nutritional value and flavor property, thus increasing its usage in food.
Article
Microbiology
Shahneela Mazhar, Ekaterina Khokhlova, Joan Colom, Annie Simon, John Deaton, Kieran Rea
Summary: Bacillus subtilis DE111((R)) is a safe and well-tolerated commercially available spore-forming probiotic that has been clinically proven to support a healthy gut microbiome and promote digestive and immune health in both adults and children. This study aimed to explore the functional properties of DE111((R)) through in vitro functional assays and genome analysis. The findings demonstrated the genetic and phenotypic ability of DE111((R)) to survive harsh gastric transit and provide health benefits to the host.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Thirumalaivasan Ramachandran, Devaprakash Manoharan, Sivakumar Natesan, Shyam Kumar Rajaram, Ponmurugan Karuppiah, Mohammed Rafi Shaik, Mujeeb Khan, Baji Shaik
Summary: In this research, selenium nanoparticles (SeNPs) were synthesized and conjugated with bacterial exopolysaccharide (EPS). The SeNPs-EPS conjugates exhibited enhanced antioxidant activity and improved growth and survival rates in experimental Artemia nauplii.
Article
Food Science & Technology
Felix Kofi Agbeko Kuebutornye, Yishan Lu, Zhiwen Wang, Jan Mraz
Summary: This study proves the probiotic traits of Bacillus velezensis TPS3N, Bacillus subtilis TPS4, and Bacillus amyloliquefaciens TPS17, which were isolated from the gut of Nile tilapia, through genomic analysis. Gene clusters responsible for acid and bile tolerance, adhesion, and colonization of the host's gut were identified. Gene markers for bacteriocins and secondary metabolites were also found, suggesting their potential use as alternatives to traditional antibiotics. In addition, the isolates were shown to enhance the host's immunity, aid in digesting complex feed ingredients, and possess genes for synthesizing vitamins and amino acids. This study provides valuable insights into these isolates and opens avenues for genetic manipulations to enhance their characteristics.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Food Science & Technology
Nawazish Ali, Zhengjun Pang, Fenghuan Wang, Baocai Xu, Hesham R. El-Seedi
Summary: Biosurfactants are a group of biomolecules with distinct surface and emulsifying ability, produced by various fungi, yeast, and bacteria. Bacillus lipopeptides, a type of biosurfactant, have diverse biological activities and find applications in food, healthcare, and agriculture.
JOURNAL OF FOOD QUALITY
(2022)
Article
Chemistry, Applied
Shujuan Meng, Yadong Wang, Jing Mu, Zhengjun Pang, Fenghuan Wang, Yonghong Liao
Summary: Surfactant-like peptides are small molecule short peptides designed by imitating natural lipid molecules, with advantages such as environmentally friendly, biodegradable, bacteriostatic, and nutritional properties. In this study, three novel surfactant-like peptides were successfully synthesized using biological methods and found to have good surface activity and low critical micelle concentration. Additionally, these peptides are pH-responsive and can self-assemble to form nanostructures in different solutions.
JOURNAL OF SURFACTANTS AND DETERGENTS
(2022)
Article
Chemistry, Applied
Asad Ullah, Malik Wajid Hussain Chan, Sadar Aslam, Ali Khan, Qamar Abbas, Shamsher Ali, Meher Ali, Abid Hussain, Zulfiqar Ali Mirani, Syed Sibt-e-Hassan, Mohib Reza Kazmi, Shaukat Ali, Shafqat Hussain, Abdul Majeed Khan
Summary: Sudan dyes in branded and non-branded spices were investigated in Karachi, Pakistan. The non-branded spices had higher concentrations of Sudan dyes than the maximum limits, while no Sudan dyes were found in the branded spices.
FOOD ADDITIVES & CONTAMINANTS PART B-SURVEILLANCE
(2023)
Article
Biochemistry & Molecular Biology
Muhammad Naveed, Yadong Wang, Xian Yin, Malik Wajid Hussain Chan, Sadar Aslam, Fenghuan Wang, Baocai Xu, Asad Ullah
Summary: In this study, a multi-step methodology was used to purify lysozyme, including salt precipitation, dialysis, and ultrafiltration. The antimicrobial activity of the purified lysozyme was found to be higher against gram-positive bacteria compared to gram-negative bacteria. The composition of the purified lysozyme was determined using LC-MS, and two peptides related to bactericidal activity were identified. Rating: 6.5 out of 10.
Article
Green & Sustainable Science & Technology
Yadong Wang, Haoran Yu, Qing Hu, Yanpeng Huang, Ximing Wang, Yuanhao Wang, Fenghuan Wang
Summary: CO2 hydrogenation to methanol process converts CO2 emissions into different chemicals using H2 produced from green energy. However, current catalysts suffer from low CO2 conversion, methanol selectivity, and deactivation. This study presents a Cu/CeZrOx solid solution catalyst prepared with ultrasound and a microimpinging stream reactor, which exhibits higher methanol production, CO2 conversion, and stability. The catalyst's superior performance is attributed to oxygen vacancies and a higher ratio of Cu+. This work provides a novel catalyst preparation method and deepens understanding of high-performance catalyst development.
Article
Biotechnology & Applied Microbiology
Xian Yin, Yu Zhou, Hulin Yang, Yonghong Liao, Tengbo Ma, Fenghuan Wang
Summary: This study successfully engineered Bacillus subtilis 168 for the microbial production of SeMCys at a titer of 18.4 μg/L, providing a good starting point for achieving industrial-scale production of SeMCys.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Asad Ullah, Muhammad Samee Haider, Malik Wajid Hussain Chan, Sadar Aslam, Lakht-e-Zehra, Omer Mukhtar Tarar, Nida Saleem, Victoria Arija
Summary: This study investigated the effects of temperature on the fatty acids profile and degree of unsaturation in Oreochromis niloticus. The results showed that temperature variations during the study period were significant and had an impact on the fatty acid composition. The degree of unsaturation of fatty acids increased with decreasing temperature. The nutritional quality of O. niloticus was found to be high, with recommended values for lipid quality indices.
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Jin Li, Sai Wen, Baoyuan Zhang, Fenghuan Wang
Summary: Selenium (Se) is a crucial nutrient in human physiology and immune function, and supplementing bioavailable Se can be beneficial for human health. The Antrodia camphorata mushroom, native to Taiwan, can assimilate and transform sodium selenite into organic selenium. The study found that the organic selenium content accounted for 88.1% of the total selenium content in selenium-enriched A. camphorata mycelia, and selenium-enriched polysaccharide was the main form of Se present in the mushroom. The selenium-enriched polysaccharides also showed a significant improvement in antioxidant capacity.
Article
Biochemistry & Molecular Biology
Asad Ullah, Zulfiqar Ali Mirani, Si Binbin, Fenghuan Wang, Malik Wajid Hussain Chan, Sadar Aslam, Liao Yonghong, Najmul Hasan, Muhammad Naveed, Shafqat Hussain, Zakia Khatoon
Summary: The present study investigated the antibacterial and anti-biofilm activity of selenium nanoparticles (SeNPs) produced by Bacillus subtilis BSN313. The SeNPs showed significant antibacterial activity against P. aeruginosa ATCC 9027, S. typhi ATCC 14028, and S. aureus ATCC 25923 when using the agar overlay method. Atomic force spectroscopy results demonstrated the disintegrating effect of SeNPs against Gram-negative and Gram-positive pathogens. The SeNPs also exhibited significant anti-biofilm activity against biofilm-producing pathogenic strains. Rating: 8/10.
PROCESS BIOCHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Xian Yin, Meiyi Zhao, Yu Zhou, Hulin Yang, Yonghong Liao, Fenghuan Wang
Summary: This study reports the significance of methylmethionine as a methyl donor for SeMCys production in B. subtilis, and highlights the importance of enhanced precursor supply in facilitating SeMCys synthesis. The results represent the highest SeMCys production to date and provide insights into Se metabolism.
MICROBIAL CELL FACTORIES
(2023)
Article
Biotechnology & Applied Microbiology
Zhengjun Pang, Yuanzi Li, Yu Shang, Nawazish Ali, Fenghuan Wang, Dianwei Zhang, Yonghong Liao
Summary: In this study, the composition of the medium for lichenysin production by Bacillus licheniformis was optimized using a factorial design, resulting in an increased titer of lichenysin.
FERMENTATION-BASEL
(2022)