Article
Microbiology
So-Young Ham, Han-Shin Kim, Min Jee Jo, Jeong-Hoon Lee, Youngjoo Byun, Gang-Jee Ko, Hee-Deung Park
Summary: The combination of 6-gingerol analog and tobramycin showed stronger inhibitory effects on biofilm formation and virulence factor production compared to individual treatments in P. aeruginosa infections. This combined treatment reduced infectivity without inducing cytotoxic effects in human lung epithelial cells. The adjuvant effects of this combination may lead to improved therapeutic development in chronic infections.
MICROBIOLOGY SPECTRUM
(2021)
Article
Respiratory System
Luca Cavinato, Francesco R. Luly, Valentina Pastore, Daniele Chiappetta, Gloria Sangiorgi, Eva Ferrara, Pia Baiocchi, Giuseppe Mandarello, Giuseppe Cimino, Paola Del Porto, Fiorentina Ascenzioni
Summary: Cystic fibrosis (CF) is characterized by chronic bacterial lung infection and inflammation due to mutations in CF transmembrane conductance regulator (CFTR). Monocytes and monocyte-derived macrophages in CF have been shown to have defective phagocytosis and antimicrobial activity against relevant pathogens. This study evaluated the effect of CFTR triple modulator therapy on CF monocyte activity against Pseudomonas aeruginosa. The results showed that CF monocytes exhibited improved phagocytic and microbicidal activity, reduced oxidative burst, and decreased inflammatory response after ETI therapy.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Infectious Diseases
Katie A. Parsels, Keri A. Mastro, Jeffrey M. Steele, Stephen J. Thomas, Wesley D. Kufel
Summary: Cefiderocol is a novel siderophore cephalosporin that has shown activity against clinically relevant MDR Gram-negative bacteria. FDA-approved indications include complicated urinary tract infections and hospital-acquired bacterial pneumonia. Clinical trials have demonstrated non-inferiority to standard treatments, but higher all-cause mortality was observed in certain cases.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Donghoon Kang, Natalia V. Kirienko
Summary: Pseudomonas aeruginosa is a multidrug-resistant pathogen that causes acute infections in immunocompromised patients, using the siderophore pyoverdine to facilitate infection and virulence. Inhibiting pyoverdine production can reduce pathogen virulence and may be a promising therapeutic target.
Article
Biochemistry & Molecular Biology
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Agnieszka Mikucka
Summary: This study assessed the susceptibility and virulence factor gene frequency of clinical P. aeruginosa strains causing bloodstream infections. The results showed that these strains were resistant to multiple antibiotics and had diverse compositions of virulence factor genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Weijun Xiu, Lili Ren, Huayu Xiao, Yue Zhang, Dou Wang, Kaili Yang, Siyu Wang, Lihui Yuwen, Xiao Li, Heng Dong, Qiang Li, Yongbin Mou, Yuqian Zhang, Zhaowei Yin, Bin Liang, Yu Gao, Lianhui Wang
Summary: An ultrasound-responsive catalytic micro-bubble is developed to combat chronic lung infections caused by Pseudomonas aeruginosa biofilms. These microbubbles physically disrupt the biofilm structure and enhance the penetration of nanoparticles and antibiotics. The nanoparticles chemically degrade the biofilm matrix and kill bacteria, while also activating the immune response. This strategy efficiently treats chronic lung infections by combining physical/chemical/antibiotic biofilm elimination and immune activation.
Article
Infectious Diseases
Lorenz Leitner, Aleksandre Ujmajuridze, Nina Chanishvili, Marina Goderdzishvili, Irina Chkonia, Sophia Rigvava, Archil Chkhotua, Giorgi Changashvili, Shawna McCallin, Marc P. Schneider, Martina D. Liechti, Ulrich Mehnert, Lucas M. Bachmann, Wilbert Sybesma, Thomas M. Kessler
Summary: This study aimed to determine the effectiveness of intravesical bacteriophage therapy in treating UTIs. Findings showed that bacteriophage therapy was non-inferior to standard antibiotic treatment, but not superior to placebo bladder irrigation in terms of efficacy or safety. The safety profile of bacteriophages appears to be favorable, suggesting potential for further large-scale clinical studies on their role in UTI treatment.
LANCET INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Jiarong Zhou, Nishta Krishnan, Zhongyuan Guo, Christian J. Ventura, Maya Holay, Qiangzhe Zhang, Xiaoli Wei, Weiwei Gao, Ronnie H. Fang, Liangfang Zhang
Summary: This study investigates the effectiveness of a biomimetic nanotoxoid vaccine in protecting immunodeficient animals from Pseudomonas aeruginosa infection. The nanotoxoids utilize a macrophage membrane coating to sequester and present bacterial virulence factors, providing rapid and long-lasting immunity. The nanovaccine can be administered through multiple routes and effectively protects against lethal infections in pneumonia and septicemia models.
Article
Infectious Diseases
Emilie Charpentier, Ludovic Doudet, Ingrid Allart-Simon, Marius Colin, Sophie C. Gangloff, Stephane Gerard, Fany Reffuveille
Summary: The combination of non-antimicrobial molecules with antibiotics can significantly reduce bacterial biofilm formation, potentially offering a solution to combat antibiotic resistance emergence.
Article
Multidisciplinary Sciences
Thomas F. Durand-Reville, Alita A. Miller, John P. O'Donnell, Xiaoyun Wu, Mark A. Sylvester, Satenig Guler, Ramkumar Iyer, Adam B. Shapiro, Nicole M. Carter, Camilo Velez-Vega, Samir H. Moussa, Sarah M. McLeod, April Chen, Angela M. Tanudra, Jing Zhang, Janelle Comita-Prevoir, Jan A. Romero, Hoan Huynh, Andrew D. Ferguson, Peter S. Horanyi, Stephen J. Mayclin, Henry S. Heine, George L. Drusano, Jason E. Cummings, Richard A. Slayden, Ruben A. Tommasi
Summary: The study presents a rational design strategy for developing new antibiotics to combat infections caused by drug-resistant Gram-negative pathogens. The inhibitors are able to overcome multiple mechanisms of resistance and show potential clinical efficacy against superbugs.
Article
Pharmacology & Pharmacy
Davoodbasha MubarakAli, Kannappan Arunachalam, Murugan Lakshmanan, Bazigha Badar, Jung-Wan Kim, Sang-Yul Lee
Summary: Biofilm-related nosocomial infections can cause various life-threatening infections. In this study, hydroxyapatite nanoparticles (HANPs) were synthesized using a co-precipitation method followed by microwave treatment, and their ability to inhibit the biofilm formation of Pseudomonas aeruginosa ATCC 10145 was investigated. The results showed that the synthesized HANPs effectively inhibited biofilm formation, suggesting their potential use in controlling biofilm-related infections caused by Pseudomonas aeruginosa.
Review
Immunology
Pamella Constantino-Teles, Albane Jouault, Lhousseine Touqui, Alessandra Mattos Saliba
Summary: Pseudomonas aeruginosa, an opportunistic pathogen, causes respiratory infections by utilizing various virulence factors, such as bacterial lipids and enzymes, and by interacting with host lipids. These mechanisms play important roles in the establishment and persistence of P. aeruginosa in the lungs, as well as in modulating host immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Engineering, Environmental
Panlin Wang, Jindi Zhao, Yongqiang Ruan, Xinyi Cai, Jin Li, Lihui Zhang, He Huang
Summary: Two strains of Pseudomonas aeruginosa were able to survive with polypropylene as the sole source of carbon, leading to weight loss and changes in the surface structure of PP.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Review
Infectious Diseases
Dana Holger, Razieh Kebriaei, Taylor Morrisette, Katherine Lev, Jose Alexander, Michael Rybak
Summary: Pseudomonas aeruginosa is a common pathogen causing healthcare-associated diseases and is highly resistant to many antibiotics, posing challenges in treatment. Bacteriophages, as viruses targeting and infecting bacterial cells, offer promise as an alternative therapy for multidrug-resistant P. aeruginosa infections. Further studies on phage pharmacokinetics and pharmacodynamics are needed to guide successful phage therapy for these infections.
Article
Immunology
Jiuxin Qu, Zhao Cai, Yumei Liu, Xiangke Duan, Shuhong Han, Jihong Liu, Yuao Zhu, Zhaofang Jiang, Yingdan Zhang, Chao Zhuo, Yang Liu, Yingxia Liu, Lei Liu, Liang Yang
Summary: The study found that a strong biofilm-forming Pseudomonas aeruginosa strain collected from a severe COVID-19 patient displayed specific epigenetic markers, which might enhance its antibiotic resistance and in vivo colonization ability in COVID-19 patients.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Engineering, Biomedical
Daniel Sanchez-Guzman, Pierre Le Guen, Berengere Villeret, Nuria Sola, Remi Le Borgne, Alice Guyard, Alix Kemmel, Bruno Crestani, Jean-Michel Sallenave, Ignacio Garcia-Verdugo
Article
Multidisciplinary Sciences
Juliette Simonin, Emmanuelle Bille, Gilles Crambert, Sabrina Noel, Elise Dreano, Aurelie Edwards, Aurelie Hatton, Iwona Pranke, Berengere Villeret, Charles-Henry Cottart, Jean-Patrick Vrel, Valerie Urbach, Nesrine Baatallah, Alexandre Hinzpeter, Anita Golec, Lhousseine Touqui, Xavier Nassif, Luis J. V. Galietta, Gabrielle Planelles, Jean-Michel Sallenave, Aleksander Edelman, Isabelle Sermet-Gaudelus
SCIENTIFIC REPORTS
(2019)
Article
Immunology
Berengere Villeret, Brigitte Solhonne, Marjolene Straube, Flora Lemaire, Aurelie Cazes, Ignacio Garcia-Verdugo, Jean-Michel Sallenave
FRONTIERS IN IMMUNOLOGY
(2020)
Review
Immunology
Jean-Michel Sallenave, Loic Guillot
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Berengere Villeret, Reem Ghinnagow, Saade Kheir, Maelys Born-Bony, Jay K. Kolls, Ignacio Garcia-Verdugo, Jean-Michel Sallenave
Summary: This study reveals the importance of lymphocytes in fighting against Pseudomonas aeruginosa infection. The bacterium down-regulates IL-23 and IL-22 protein accumulation in the lungs, but up-regulates their RNA production. Adenovirus-mediated over-expression of IL-1, IL-23, and IL-7 can rescue mice from Pseudomonas aeruginosa-induced lethality, suggesting its potential value in locally immunosuppressed individuals like cystic fibrosis patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lisa Prince, Lydie Martin-Faivre, Berengere Villeret, Daniel Sanchez-Guzman, Pierre Le Guen, Jean-Michel Sallenave, Ignacio Garcia-Verdugo
Summary: Eosinophils play a crucial role in inducing a complete vaccine response, promoting antibody secretion in the early stage of the immune response. They migrate to the lungs and secrete cytokines to activate B cells, promoting antibody production. However, the absence of eosinophils does not impair late immune responses due to a compensating mechanism involving the Th17 pathway. This study reveals a novel role for eosinophils in lung mucosal vaccination, accelerating early immune responses while regulating IgA production in the late stages.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Oncology
Irati Garmendia, Aditi Varthaman, Solenne Marmier, Mahmud Angrini, Ingrid Matchoua, Aurelie Darbois-Delahousse, Nathalie Josseaume, Pierre-Emmanuel Foy, Lubka T. Roumenina, Naira Naouar, Maxime Meylan, Sophie Siberil, Jules Russick, Pierre-Emmanuel Joubert, Karen Leroy, Diane Damotte, Audrey Mansuet-Lupo, Marie Wislez, Marco Alifano, Laurie Menger, Ignacio Garcia-Verdugo, Jean-Michel Sallenave, Olivier Lantz, Florent Petitprez, Isabelle Cremer
Summary: We found that acute influenza A virus infection has a significant impact on the tumor microenvironment and clinical outcomes of patients with non-small cell lung cancer. Influenza virus can infect both tumor and immune cells, resulting in long-term protumoral effects. Infection with influenza virus alters the transcriptional profile of the tumor microenvironment and is associated with poor survival in patients with lung adenocarcinoma.
CANCER IMMUNOLOGY RESEARCH
(2023)
Meeting Abstract
Pediatrics
S. Kheir, B. Villeret, I Garcia-Verdugo, J. Sallenave
PEDIATRIC PULMONOLOGY
(2020)
Meeting Abstract
Pediatrics
J. Sallenave, B. Villeret, B. Solhonne, M. Straube, A. Cazes, I Garcia-Verdugo
PEDIATRIC PULMONOLOGY
(2019)