Article
Biochemistry & Molecular Biology
Hung-Chun Liao, Wan-Ling Wu, Chen-Yi Chiang, Min-Syuan Huang, Kuan-Yin Shen, Yu-Ling Huang, Suh-Chin Wu, Ching-Len Liao, Hsin-Wei Chen, Shih-Jen Liu
Summary: During the COVID-19 pandemic, global mass vaccination is crucial for achieving herd immunity. The study shows that the S-Trimer vaccine, when adjuvanted with SWE, induces strong antibody and cellular immune responses, providing neutralizing antibodies against the original and variant strains of SARS-CoV-2. Additionally, the SWE adjuvant allows for a lower vaccine dosage while maintaining effectiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Galit Alter, Jingyou Yu, Jinyan Liu, Abishek Chandrashekar, Erica N. Borducchi, Lisa H. Tostanoski, Katherine McMahan, Catherine Jacob-Dolan, David R. Martinez, Aiquan Chang, Tochi Anioke, Michelle Lifton, Joseph Nkolola, Kathryn E. Stephenson, Caroline Atyeo, Sally Shin, Paul Fields, Ian Kaplan, Harlan Robins, Fatima Amanat, Florian Krammer, Ralph S. Baric, Mathieu Le Gars, Jerald Sadoff, Anne Marit de Groot, Dirk Heerwegh, Frank Struyf, Macaya Douoguih, Johan van Hoof, Hanneke Schuitemaker, Dan H. Barouch
Summary: The Ad26.COV2.S vaccine has shown clinical efficacy against symptomatic COVID-19, including the B.1.351 variant, but there is uncertainty regarding its immunogenicity against SARS-CoV-2 variants. The study found that neutralizing antibody responses were reduced against the B.1.351 and P.1 variants, while non-neutralizing antibody responses and T cell responses were largely preserved against SARS-CoV-2 variants.
Review
Biochemistry & Molecular Biology
Mandeep Kaur, Akanksha Sharma, Santosh Kumar, Gurpal Singh, Ravi P. Barnwal
Summary: Globally, SARS-CoV-2 poses a threat to life and economy, prompting researchers to search for a cure. This review aims to summarize the functional roles of different domains of the SARS-CoV-2 genome and highlight the importance of finding potential drugs/vaccines.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Clinical Neurology
Nicol G. M. Oonk, Anne Rozemarijn Ettema, Heleen van Berghem, Jabke J. de Klerk, Joyce P. M. van der Vegt, Matthijs van der Meulen
Summary: This study describes three cases of neurological symptoms after SARS-CoV-2 vaccination and proposes possible pathophysiological mechanisms. Thunderclap headache may be related to the activation of immunoinflammatory mediators or fever, while the progression of post-infectious myoclonus may be associated with auto-immune mediated crossreaction. Further research is needed to understand the pathophysiological mechanisms of the SARS-CoV-2 vaccine and its association with neurological symptoms.
NEUROLOGICAL SCIENCES
(2022)
Article
Immunology
Pedro J. Alcolea, Jaime Larraga, Daniel Rodriguez-Martin, Ana Alonso, Francisco J. Loayza, Jose M. Rojas, Silvia Ruiz-Garcia, Andres Louloudes-Lazaro, Ana B. Carlon, Pedro J. Sanchez-Cordon, Pablo Nogales-Altozano, Natalia Redondo, Miguel Manzano, Daniel Lozano, Jesus Palomero, Maria Montoya, Maria Vallet-Regi, Veronica Martin, Noemi Sevilla, Vicente Larraga
Summary: Researchers presented a promising DNA vaccine candidate, pPAL-Sfs + pPAL-N, which demonstrated strong protective effects in mice, fully controlling viral replication and inducing potent humoral and cellular immune responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Eva Fisher, Laura Padula, Kristin Podack, Katelyn O'Neill, Matthew M. Seavey, Padmini Jayaraman, Rahul Jasuja, Natasa Strbo
Summary: This study leveraged cellular heat shock chaperone protein, gp96, to deliver SARS-CoV-2 protein S to the immune system, inducing cell-mediated immune responses. The vaccine platform effectively stimulated a robust cellular immune response against protein S, showing promising translational data for potential use in humans against SARS-CoV-2 antigen presentation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Neil C. Dalvie, Sergio A. Rodriguez-Aponte, Brittany L. Hartwell, Lisa H. Tostanoski, Andrew M. Biedermann, Laura E. Crowell, Kawaljit Kaur, Ozan S. Kumru, Lauren Carter, Jingyou Yu, Aiquan Chang, Katherine McMahan, Thomas Courant, Celia Lebas, Ashley A. Lemnios, Kristen A. Rodrigues, Murillo Silva, Ryan S. Johnston, Christopher A. Naranjo, Mary Kate Tracey, Joseph R. Brady, Charles A. Whittaker, Dongsoo Yun, Natalie Brunette, Jing Yang Wang, Carl Walkey, Brooke Fiala, Swagata Kar, Maciel Porto, Megan Lok, Hanne Andersen, Mark G. Lewis, Kerry R. Love, Danielle L. Camp, Judith Maxwell Silverman, Harry Kleanthous, Sangeeta B. Joshi, David B. Volkin, Patrice M. Dubois, Nicolas Collin, Neil P. King, Dan H. Barouch, Darrell J. Irvine, J. Christopher Love
Summary: Subunit vaccines based on recombinant proteins are crucial tools for controlling COVID-19 due to their low manufacturing cost, scalability, and proven safety and efficacy. Engineered RBD variants exhibit high manufacturability and immunogenicity, showing enhanced immune responses after a single dose and cross-reactivity to new variants of concern.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Chia-En Lien, Tsun-Yung Kuo, Yi-Jiun Lin, Wei-Cheng Lian, Meei-Yun Lin, Luke Tzu-Chi Liu, Jinyi Cheng, Yu-Chi Chou, Charles Chen
Summary: The CpG 1018-adjuvanted S-2P subunit vaccine MVC-COV1901 induced neutralizing antibodies against SARS-CoV-2 variants of concern in both rats and humans, with higher doses leading to stronger immune responses. Despite significant neutralization abilities against the D614G and Alpha variants, neutralizing titers against the Beta variant were notably reduced in vaccinated subjects. Additional doses, especially in rats, were able to partially compensate for this reduction in neutralization against the Beta variant.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Immunology
Antonella Scaglione, Silvana Opp, Alicia Hurtado, Ziyan Lin, Christine Pampeno, Maria G. Noval, Sara A. Thannickal, Kenneth A. Stapleford, Daniel Meruelo
Summary: This study introduces a novel and highly effective vaccine approach that provides long-term protective immunity by activating T cells. The vaccine shows strong immune protection against coronavirus infection in transgenic mice. The results suggest that this vaccine may be effective against challenging variants and could serve as a platform for developing a broader spectrum pancoronavirus vaccine.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Lais Canniatti Brazaca, Amanda Hikari Imamura, Nathalia Oezau Gomes, Mariana Bortholazzi Almeida, Desiree Tamara Scheidt, Paulo A. Raymundo-Pereira, Osvaldo N. Oliveira Jr, Bruno Campos Janegitz, Sergio Antonio Spinola Machado, Emanuel Carrilho
Summary: This study reports the development of a low-cost immunosensor based on gold-modified screen-printed carbon electrodes for the fast and sensitive determination of S proteins from SARS-CoV and SARS-CoV-2. The immunosensor showed high selectivity, sensitivity, and a low limit of detection, allowing for direct determination of target proteins in saliva samples. The immunoplatform presented in this study has the potential for mass testing in point-of-care settings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Ehsan Mostafavi, Sana Eybpoosh, Mohammad Karamouzian, Malahat Khalili, Saiedeh Haji-Maghsoudi, Mostafa Salehi-Vaziri, Ali Khamesipour, Tahmineh Jalali, Mehran Nakhaeizadeh, Hamid Sharifi, Yasaman Mansoori, Fariba Keramat, Samad Ghodrati, Mostafa Javanian, Delaram Doroud, Mir Davood Omrani, Hassan Asadi, Mohammad Hassan Pouriayevali, Roya Ghasemian, Hossein Farshidi, Morteza Pourahmad, Iman Ghasemzadeh, Leila Mounesan, Maryam Darvishian, Mohamad Reza Mirjalili, Maria Eugenia Toledo-Romani, Carmen Valenzuela-Silva, Vicente Verez-Bencomo, Mohammad Mehdi Gouya, Hamid Emadi-Koochak, Ali Akbar Haghdoost, Alireza Biglari
Summary: This article evaluates the efficacy and safety of FINLAY-FR-2 and FINLAY-FR-1A vaccines in the Iranian population. The results show that both vaccines have acceptable vaccine efficacy against COVID-19 infection and are safe to administer. Therefore, Soberana may be a choice for mass vaccination, especially in resource-limited settings.
Article
Immunology
Wonsuck Yoon, Yongsung Park, Seunghyun Kim, Iel Soo Bang
Summary: This study evaluated the potential of an oral bacteria-based vaccine as a safe and effective platform for large-scale, long-term immunization. Engineered Salmonella cells expressing viral proteins related to COVID-19 were used to develop an oral vaccine candidate, which showed promising results in promoting antigen-specific antibody production and inducing cellular immunity. These findings suggest that Salmonella strains could be a valuable platform for developing an oral vaccine alternative to combat COVID-19 and future infectious disease outbreaks.
Article
Multidisciplinary Sciences
Yi-Jiun Lin, Meei-Yun Lin, Ya-Shan Chuang, Luke Tzu-Chi Liu, Tsun-Yung Kuo, Charles Chen, Shyamala Ganesan, Ali Fattom, Vira Bitko, Chia-En Lien
Summary: Intramuscular vaccines do not address the issue of persistent viral carriage and transmission, but intranasal booster vaccines following intramuscular vaccination can promote protective immunity against viral infection and disease.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Lu Zhang, Ruifang Cao, Tiantian Mao, Yuan Wang, Daqing Lv, Liangfu Yang, Yuanyuan Tang, Mengdi Zhou, Yunchao Ling, Guoqing Zhang, Tianyi Qiu, Zhiwei Cao
Summary: Since the outbreak of SARS-CoV-2, concerns regarding antigenicity have been persistent due to emerging mutants. A platform named SAS has been developed to predict the resistant effect of emerging variants and the dynamic coverage of SARS-CoV-2 antibodies among circulating strains, providing valuable insights for vaccine effectiveness and epidemiological strategies. The SAS prediction results have shown good consistency with experimental tests, offering a potential tool to complement targeted immune-binding experiments and track the emergence of antigenic variants.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Immunology
Seyede Atefe Hosseini, Fatemeh Zahedipour, Hamed Mirzaei, Reza Kazemi Oskuee
Summary: Since September 2020, there have been over 28 million cases of COVID-19 worldwide, with many countries now facing a second wave of the outbreak. The urgent need for a potent vaccine against SARS-CoV-2 is evident, and institutions and companies in many countries are actively working on vaccine research programs and progress. Challenges in vaccine development include limited knowledge about the virus, inducing immune responses, animal models, and ensuring safety and efficacy criteria are met.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Xin Wang, Di Zhao, Narasimha Murthy Beeraka, Spandana Tatineni, Chiriki Devi Sri, Veera Venkata Nishanth, Chinnappa Apatira Uthiah, Zonunsiami Leihang, Kavya Sugur, Junqi Liu, Vladimir Nikolaevich Nikolenko, Ruitai Fan
Summary: This review discusses the role of epigenetics in cardiometabolic diseases and insulin signaling, and explores the frontier research on the development of epigenetic biomarkers and chromatin-based therapies. The study highlights a range of epigenetic alterations that are associated with oxidative stress, insulin signaling, metabolic cardiomyopathy, and endothelial dysfunction, which can regulate gene transcription and affect inflammation, lipogenesis, and adipogenesis. Furthermore, several drugs show potential as epigenetic modulators. Unraveling cell-specific epigenetic information is crucial for individual risk assessment and the development of chromatin-modifying therapies in patients with cardiometabolic diseases.
CURRENT MEDICINAL CHEMISTRY
(2023)
Review
Obstetrics & Gynecology
Viktor V. Smirnov, Narasimha M. Beeraka, Dmitry Yu Butko, Vladimir N. Nikolenko, Sergey A. Bondarev, Evgeniy E. Achkasov, Mikhail Y. Sinelnikov, P. R. Hemanth Sinelnikov
Summary: This study aims to investigate the impact of endocrine variables, hyperandrogenism, insulin resistance, oxidative stress, and dietary conditions on polycystic ovarian syndrome (PCOS), as well as to discuss the role of epigenetic factors in PCOS conditions. The comparative therapeutic effects of various PCOS therapies and the importance of metabolic/endocrine variables in PCOS were described. The study also discussed the efficacy of GLP-1 agonists, insulin sensitizers, and diet/exercise regimens in enhancing fertility outcomes in overweight or obese females with PCOS. Furthermore, the study highlighted the significance of DNA methylation as epigenetic changes and the potential for developing chromatin-modifying therapies against PCOS through screening molecular targets.
REPRODUCTIVE SCIENCES
(2023)
Correction
Obstetrics & Gynecology
Viktor V. Smirnov, Narasimha M. Beeraka, Dmitry Yu Butko, Vladimir N. Nikolenko, Sergey A. Bondarev, Evgeniy E. Achkasov, Mikhail Y. Sinelnikov, P. R. Hemanth Vikram
REPRODUCTIVE SCIENCES
(2023)
Review
Oncology
Junqi Liu, Jin Zhang, Narasimha M. M. Beeraka, Kuo Chen, Mikhail Y. Y. Sinelnikov, Prasath Manogaran, Gurupadayya Bannimath, Vladimir N. N. Nikolenko, Ruitai Fan
Summary: miRNAs play a significant role in cancer proliferation, mitigation, and drug resistance. Nanocarriers carrying tumor-targeting miRNAs are a promising strategy to modulate stemness and overcome the limitations of low penetrability, minimal stability, and nonspecificity. Specific nanocarrier formulations with specific miRNAs can efficiently deliver therapeutic effects in melanomas by targeting tumor properties.
PIGMENT CELL & MELANOMA RESEARCH
(2023)
Review
Microbiology
Siva Dallavalasa, SubbaRao V. Tulimilli, Janhavi Prakash, Ramya Ramachandra, SubbaRao V. Madhunapantula, Ravindra P. Veeranna
Summary: The impact of COVID-19 on people with diabetes is still being researched, but there is emerging evidence that diabetes patients are more susceptible to the virus and experience more severe symptoms. The interaction between COVID-19 and diabetes is bidirectional, with the virus increasing viral entry into cells and reducing immunity in diabetes patients. Diabetes is a common complication in COVID-19 patients, leading to serious consequences such as acute respiratory distress syndrome and multi-organ failure. Further studies are needed to confirm diabetes as a complication of COVID-19.
Article
Biochemistry & Molecular Biology
Tenzin Adon, Dhivya Shanmugarajan, Hissana Ather, Shaik Mohammad Asif Ansari, Umme Hani, SubbaRao V. V. Madhunapantula, Yogish Kumar Honnavalli
Summary: CDK4/6 and aromatase are key targets for breast cancer treatment. Resistance to aromatase inhibitors can occur through alternative signaling pathways. This study aims to identify molecules that can inhibit both CDK4/6 and aromatase using computational chemistry techniques. Through screening and docking, four potential candidates were selected and their stability and effectiveness were further confirmed through molecular dynamics simulations and calculations. These computational approaches provide potential for developing dual inhibitors for breast cancer therapy.
Article
Oncology
Xin Wang, Qianqian Lou, Tianli Fan, Qing Zhang, Xiangxiang Yang, Hongtao Liu, Ruitai Fan
Summary: Increasing evidence has shown that Ctr1 plays an important role in regulating cisplatin uptake in various tumors. This study aimed to investigate its role in mediating cisplatin sensitivity in ESCC cells. Ctr1 expression was higher in ESCC tissues and cells compared to normal tissues. Furthermore, Ctr1 depletion reduced the suppressive effects of cisplatin on proliferation, migration, invasion, and apoptosis in ESCC cells, while Ctr1 upregulation combined with cisplatin exerted synergistic effects on these cellular processes.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Chemistry, Analytical
P. R. Hemanth Vikram, Narasimha M. M. Beeraka, Hitesh B. B. Patel, Pramod Kumar
Summary: In this study, a novel, simple, sensitive, accurate, cost-effective, precise, and robust RP-HPLC-DAD method was developed to quantify L-lysine hydrochloride in bulk drug substances and multivitamin oral suspension. The method does not require sample preparation and pretreatment. The analysis can be performed with an available C8 column and HPLC-UV detector, proving its cost-effectiveness compared to currently available methods for L-lysine-based products analysis.
ACCREDITATION AND QUALITY ASSURANCE
(2023)
Article
Medicine, Research & Experimental
Hitesh Kumar, Pallavi Chand, Shantanu Pachal, Sahid Mallick, Rupshee Jain, SubbaRao V. Madhunapantula, Vikas Jain
Summary: Fisetin-loaded nanostructured lipid carriers (NLCs) were developed and optimized for better efficacy against metastatic melanoma. The NLCs showed suitable particle size, stability, sustained drug release, and significantly enhanced antitumor activity in cellular and animal experiments.
MOLECULAR PHARMACEUTICS
(2023)
Article
Oncology
Xiaohong Guo, Li Wang, Narasimha M. Beeraka, Chunying Liu, Xiang Zhao, Runze Zhou, Huiming Yu, Ruitai Fan, Junqi Liu
Summary: This study analyzed the incidence trend of retinoblastoma from 2004 to 2015 and constructed a nomogram to predict overall survival in children.
Article
Multidisciplinary Sciences
Hao Gu, Tingxuan Li, Narasimha M. Beeraka, Yufei Zheng, Xintan Zhang, Ruixia Song, Runze Zhou, Xiaoyan Wang, Olga Sukocheva, Ruitai Fan, Junqi Liu
Summary: By classifying HPV-negative HNSCCs based on cell cycle-related genes, this study identified two subtypes with differences in cell cycle activity, tumor microenvironment, N stage, pathological grade, prognosis, and treatment response. The findings provide new insights for personalized anti-cancer treatment.
Article
Oncology
Prashanth Kumar M. Veeresh, Chaithanya G. Basavaraju, Siva Dallavalasa, Preethi G. Anantharaju, Suma M. Natraj, Olga A. Sukocheva, SubbaRao V. Madhunapantula
Summary: The burden of breast cancer is increasing rapidly, and it is important to develop better treatment strategies. Recent studies have found that low levels of vitamin D may be effective in reducing the cancer burden. However, the molecular mechanisms by which vitamin D induces cancer cell death are not fully understood. This study showed that vitamin D inhibited the proliferation of breast cancer cell lines in vitro and slowed down the growth of breast cancer in mice. The cytotoxic effects of vitamin D were associated with growth inhibition, induction of apoptosis, cell cycle arrest, and inhibition of angiogenesis.
Article
Medicine, General & Internal
Ruitai Fan, Yufei Zheng, Runze Zhou, Narasimha M. Beeraka, Olga A. Sukocheva, Ruiwen Zhao, Shijie Li, Xiang Zhao, Chunying Liu, Song He, P. A. Mahesh, B. M. Gurupadayya, Vladimir N. Nikolenko, Di Zhao, Junqi Liu
Summary: This study evaluated the trial registration status on the Chinese Clinical Trial Registry (ChiCTR). The results showed a consistent upward trend in clinical trial registrations, with a focus on oncological and cardiovascular diseases and public funding as the major source of funding.
FRONTIERS IN MEDICINE
(2023)
Article
Materials Science, Biomaterials
Sharon George, Asha Srinivasan, SubbaRao V. Tulimilli, SubbaRao V. Madhunapantula, Shajesh Palantavida
Summary: Photothermal therapy utilizes photothermal agents and the use of nanoparticle agents is advantageous. Common nanophotothermal agents have high conversion efficiencies and heating rates, but bulk temperature methods do not represent their nanoscale temperatures. This study reports on the fabrication of self-limiting hyperthermic nanoparticles that can induce hyperthermia and measure temperature ratiometrically. These nanoparticles utilize a plasmonic core for hyperthermia and fluorescent FRET pairs for temperature sensing.
JOURNAL OF MATERIALS CHEMISTRY B
(2023)
Article
Chemistry, Multidisciplinary
Prem K. Sreenivasan, Bhojraj Nandlal, Ganganna Aruna, SubbaRao V. Madhunapantula
Summary: This investigation demonstrates the use of biochemical quantitation and a rapid visual test for sialic acid as a measure of oral health. The results show that salivary sialic acid levels correlate with clinical status and can be used for screening and monitoring oral health.
APPLIED SCIENCES-BASEL
(2023)
