Journal
MOLECULAR BIOLOGY REPORTS
Volume 48, Issue 5, Pages 3979-3989Publisher
SPRINGER
DOI: 10.1007/s11033-021-06398-7
Keywords
miR-9-5p; Galectin-3; Oral cavity cancer; MMP; AKT; γ -catenin
Categories
Funding
- Cancer Australia Grant [APP1145657]
- NHMRC [APP 2002576]
- National Institutes of Health [1R21EB030349-01]
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The study found that overexpression of miR-9-5p in oral cavity cancer leads to increased apoptosis, reduced cell proliferation and migration, and increased secretion and export of Galectin-3. MiR-9-5p may modulate Galectin-3 via the AKT/gamma-catenin pathway. The positive correlation between the expression levels of miR-9-5p and secreted Galectin-3 in saliva highlights their importance in the progression of oral cavity cancer.
Oral cavity cancer (OCC) is the predominant subtype of head and neck cancer (HNC) and has up to 50% mortality. Genome-wide microRNA (miR) sequencing data indicates overexpression of miR-9-5p in HNC tumours, however, the biological role of miR-9-5p in OCC is complex; it can either act as a tumour suppressor or an oncomir, regulating many target genes at the post-transcriptional level. We have investigated the overexpression of miR-9-5p in three OCC cell lines. We have evaluated its expression levels and Galectin-3 as potential biomarkers in saliva samples collected from controls and OCC patients. We found that over expression of miR-9-5p in OCC cell lines resulted in a significant reduction in cell proliferation and migration, and an increase in apoptosis, which was paralleled by an increase in Galectin-3 secretion and export of Galectin-3 protein. Our data are consistent with miR-9-5p being a modulator of Galectin-3 via the AKT/gamma-catenin pathway. In addition, the positive correlation between the levels of miR-9-5p expression and secreted Galectin-3 in saliva reflects a similar relationship in vivo, and supports the utility of their integrative evaluation in OCC. Our findings indicate that both miR-9-5p and Galectin-3 are critical biomolecules in the progression of OCC.
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