Journal
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
Volume 127, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.msec.2021.112202
Keywords
Tilapia skin; Decellularization; Wound healing; Degradation; Re-epithelialization
Categories
Funding
- National Major Research Program of China [2016YFA0201702, 2016YFA0201700]
- Fundamental Research Funds for the Central Universities [2232019A307]
- National Natural Science Foundation of China [31771023]
- Science and Technology Commission of Shanghai Municipality [19441902600, 20DZ2254900, 20S31900900]
- Sino German Science Foundation Research Exchange Center [M0263]
- Key Research and Development Program of Shandong Province [2019JZZY011103]
- Yantai Double Hundred Talent Plan
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The tilapia-skin acellular dermal matrix (TS-ADM) was prepared through alkaline decellularization and gamma-irradiation, effectively removing nuclear components and exhibiting superior morphology, thermal stability, degradability, and water vapor transmission compared to commercial porcine acellular dermal matrix (DC-ADM). TS-ADM showed enhanced degradation both in vitro and in vivo, and significantly promoted granulation growth, collagen deposition, angiogenesis, and re-epithelialization in rat and mini-pig skin wound healing experiments, possibly due to the high expression of TGF-beta 1, alpha-SMA, and CD31.
Acellular Dermal Matrix (ADM) is mainly made with human or porcine skins and has the risk of zoonotic virus transmission. The fish skin-derived ADM could overcome the shortcoming. Fish skin acellular matrix has been used as wound dressing, but there is few systematic studies on tilapia-skin acellular dermal matrix (TS-ADM). In the present study, a novel TS-ADM was made by an alkaline decellularization process and gamma-irradiation. The physical properties, biocompatibility, pre-clinical safety and wound healing activity of TS-ADM were systematically evaluated for its value as a functionally bioactive wound dressing. Histopathological analysis (hematoxylin and eosin staining, 4,6-diamidino-2-phenylindole (DAPI) staining) and DNA quantification both proved that the nuclear components of tilapia skin were removed sufficiently in TS-ADM. Compared to the commercial porcine acellular dermal matrix (DC-ADM), TS-ADM has distinctive features in morphology, thermal stability, degradability and water vapor transmission. TS-ADM was more readily degradable than DC-ADM in vitro and in vivo. In both rat and mini-pig skin wound healing experiments, TS-ADM was shown to significantly promote granulation growth, collagen deposition, angiogenesis and re-epithelialization, which may be attributed to the high expression of transforming growth factor-beta 1 (TGF-beta 1), alpha-smooth muscle actin (alpha-SMA) and CD31. Herein, the novel TS-ADM, used as a low-cost bioactive dressing, could form a microenvironment conducive to wound healing.
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