Article
Plant Sciences
Younes Dellero, Solenne Berardocco, Cecilia Berges, Olivier Filangi, Alain Bouchereau
Summary: The estimation of metabolic fluxes in photosynthetic organisms is a significant challenge, especially with the development of C-13-Metabolic Flux Analysis. To accurately measure Carbon Isotopologue Distribution and minimize uncertainties for the calculated fluxes, the accuracy of GC-MS at the isotopologue level needs to be evaluated. This study developed a workflow to validate GC-MS measurements of Carbon Isotopologue Distributions in plant metabolites by using standardized extracts and investigated the light/dark regulation of the TCA cycle in Brassica napus leaf discs. The findings showed the cyclic operation of the TCA cycle under both light and dark conditions, with interesting contributions of different pathways and reassimilation of carbon derived from mitochondrial enzymes.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Ahmed Elshobaky, Cathrine Lillo, Kristian Persson Hoden, Amr R. A. Kataya
Summary: Protein phosphatase 2A (PP2A) is a conserved serine/threonine phosphatase complex that plays a role in substrate specificity and localization in plants. In this study, the interactions between the PP2A-B'& zeta; subunit and enzymes related to mitochondrial energy flow were investigated. Through experiments in tobacco and onion cells, interactions were confirmed between the B'& zeta; subunit and several Krebs cycle proteins. Putative interacting candidates were also deduced from phosphoproteome analysis, shedding light on the role of PP2A in regulating energy metabolism in mitochondria.
Article
Endocrinology & Metabolism
Chris E. Shannon, Mukundan Ragavan, Juan Pablo Palavicini, Marcel Fourcaudot, Terry M. Bakewell, Ivan A. Valdez, Iriscilla Ayala, Eunsook S. Jin, Muniswamy Madesh, Xianlin Han, Matthew E. Merritt, Luke Norton
Summary: Insulin resistance and altered hepatic mitochondrial function are key features of type 2 diabetes (T2D) and non-alcoholic fatty liver disease (NAFLD). This study found that insulin sensitization with pioglitazone can mitigate mitochondrial dysfunction in the liver, which is independent of changes in hepatic lipid accumulation. The research highlights potential therapeutic targets for T2D and NAFLD, emphasizing the benefits of insulin sensitization in managing obesity-related diseases.
MOLECULAR METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Tianyang Lan, Kang Zhang, Feifei Lin, Qifu He, Shenghui Wu, Zhiming Xu, Yong Zhang, Fusheng Quan
Summary: MICU1 modulators enhance mitochondrial activity, pyruvate metabolism, and developmental competence of thawed oocytes after vitrification. Vitrification leads to adverse effects on mitochondrial function, but these effects can be mitigated by MICU1 activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Ella F. Whittle, Madison Chilian, Ehsan Ghayoor Karimiani, Helga Progri, Daniela Buhas, Melis Kose, Rebecca D. Ganetzky, Mehran Beiraghi Toosi, Paria Najarzadeh Torbati, Reza Shervin Badv, Ivan Shelihan, Hui Yang, Houda Zghal Elloumi, Sukyeong Lee, Yalda Jamshidi, Alan M. Pittman, Henry Houlden, Erika Ignatius, Shamima Rahman, Reza Maroofian, Wan Hee Yoon, Christopher J. Carrol
Summary: This study aimed to identify the genetic cause of a novel autosomal recessive neurodevelopmental disorder characterized by global developmental delay, movement disorder, and metabolic abnormalities. Through clinical characterization and genetic analysis, the researchers identified three novel homozygous variants in the OGDH gene. Functional studies demonstrated that these variants interfered with the structure and function of the OGDH protein, leading to the observed neurodevelopmental disorder. This research highlights the importance of studying genetic causes of neurodevelopmental disorders for understanding their underlying mechanisms and developing targeted therapies.
GENETICS IN MEDICINE
(2023)
Article
Microbiology
Lea Sundermeyer, Jan-Gerrit Folkerts, Benita Lueckel, Christina Mack, Meike Baumgart, Michael Bott
Summary: This study demonstrates a unique localization of an enzyme complex in actinobacteria, the PDH-ODH complex. The complex is found to be localized at distinct spots at the cell poles and also at mid-cell. The localization is likely caused by nucleoid exclusion. The composition of the complex differs from that in non-actinobacterial cells.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Konstantin S. Plokhikh, Semen V. Nesterov, Yuriy M. Chesnokov, Anton G. Rogov, Roman A. Kamyshinsky, Aleksandr L. Vasiliev, Lev S. Yaguzhinsky, Raif G. Vasilov
Summary: In this study, cryo-electron tomography was used to investigate the localization of 2-oxoacid dehydrogenase complexes (OADCs) in cardiac mitochondria and their interaction with respirasomes. The results showed that OADCs were localized in densely packed lamellar cristae of cardiac mitochondria and in ruptured mitochondrial samples. This study highlights the importance of understanding mitochondrial complex coupling.
Article
Microbiology
Lea Sundermeyer, Jan-Gerrit Folkerts, Benita Lueckel, Christina Mack, Meike Baumgart, Michael Bott
Summary: This study provides an example of a unique enzyme complex, the hybrid pyruvate/2-oxoglutarate dehydrogenase complex (PDH-ODH), found in actinobacteria, with a spatially defined localization. The complex was observed to be co-localized in distinct spots at the cell poles and additional spots at mid-cell. These findings support the presence of the hybrid complex and suggest a similar localization in other actinobacteria.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cardiac & Cardiovascular Systems
Qinfeng Li, Chao Li, Abdallah Elnwasany, Gaurav Sharma, Yu A. An, Guangyu Zhang, Waleed M. Elhelaly, Jun Lin, Yingchao Gong, Guihao Chen, Meihui Wang, Shangang Zhao, Chongshan Dai, Charles D. Smart, Juan Liu, Xiang Luo, Yingfeng Deng, Lin Tan, Shuang-Jie Lv, Shawn M. Davidson, Jason W. Locasale, Philip L. Lorenzi, Craig R. Malloy, Thomas G. Gillette, Matthew G. Vander Heiden, Philipp E. Scherer, Luke I. Szweda, Guosheng Fu, Zhao V. Wang
Summary: PKM1 plays a crucial role in maintaining a homeostatic response in the heart under hemodynamic stress, protecting against cardiac dysfunction and fibrosis caused by pressure overload. Furthermore, PKM1 is essential for enhancing glycolytic flux, mitochondrial respiration, and ATP production under pressure overload at a mechanistic level.
Review
Medicine, Research & Experimental
Russel J. Reiter, Ramaswamy Sharma, Carmen Rodriguez, Vanesa Martin, Sergio Rosales-Corral, Debora Aparecida Pires de Campos Zuccari, Luiz Gustavo de Almeida Chuffa
Summary: This paper explores the relationship between endogenous melatonin and solid tumors, highlighting the potential of using melatonin to influence cancer cell metabolism. It suggests that solid tumors exhibit a healthier metabolic state at night, potentially reducing proliferation and invasiveness. The paper also proposes using melatonin in a time-sensitive treatment paradigm and combining it with traditional chemotherapy for improved outcomes in cancer treatment.
Review
Oncology
Ling-Chu Chang, Shih-Kai Chiang, Shuen-Ei Chen, Mien-Chie Hung
Summary: This review describes the molecular interactions, regulation, and disease relationships of OGDHc, a key enzyme in the tricarboxylic acid (TCA) cycle. Dysregulation of OGDHc can disrupt cellular redox homeostasis and lead to the accumulation of oncometabolites in cancer development. The potential of OGDHc as a therapeutic target for cancer treatment is discussed.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Theodoros Eleftheriadis, Georgios Pissas, Spyridon Golfinopoulos, Maria Efthymiadi, Vassilios Liakopoulos, Ioannis Stefanidis
Summary: Inhibition of malate dehydrogenase-2 (MDH-2) protects renal cells from death or senescence caused by ischemia-reperfusion injury. MDH-2 inhibition reduces oxidative stress and inflammation, and maintains cell proliferation capacity.
Article
Biochemistry & Molecular Biology
Ayano Komine-Abe, Naoko Kondo, Shosei Kubo, Hisashi Kawasaki, Makoto Nishiyama, Saori Kosono
Summary: In Corynebacterium glutamicum, acetylation of the lysine residue at position 391 on CgE2 was found to play a critical role in the assembly of PDH and ODH complexes, affecting their activities. Substitution of Lys391 with arginine in the CgE2-K391R variant strain led to increased l-glutamate production and reduced pyruvate accumulation, suggesting potential advantages for enhancing production efficiency.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Article
Biology
Ioannis Skalidis, Fotis L. Kyrilis, Christian Tueting, Farzad Hamdi, Toni K. Traeger, Jaydeep Belapure, Gerd Hause, Marta Fratini, Francis J. O'Reilly, Ingo Heilmann, Juri Rappsilber, Panagiotis L. Kastritis
Summary: This study reveals the organization, 3D architecture, and molecular function of a thermophilic, eukaryotic, native OGDHc in its active state. The results demonstrate various structural adaptations of the OGDHc core and the presence of a flexible subunit connecting E2o and E3. This research provides a blueprint for structure-function studies of complex mixtures of medical and biotechnological value.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Valeria Scalcon, Alessandra Folda, Maria Giovanna Lupo, Federica Tonolo, Naixuan Pei, Ilaria Battisti, Nicola Ferri, Giorgio Arrigoni, Alberto Bindoli, Arne Holmgren, Lucia Coppo, Maria Pia Rigobello
Summary: This study reveals the specific role of mitochondrial Grx2 in the regulation of cell metabolism and highlights an unknown association between Grx2 and metabolic dysfunction-associated fatty liver disease (MAFLD).
Article
Biochemistry & Molecular Biology
Adrian Young, Robert Gill, Ryan J. Mailloux
CHEMICO-BIOLOGICAL INTERACTIONS
(2019)
Article
Biochemistry & Molecular Biology
Ibrahim Dogar, Sarah Dixon, Robert Gill, Adrian Young, Sarah Mallay, Catherine Oldford, Ryan J. Mailloux
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Adrian Young, Catherine Oldford, Ryan J. Mailloux
Review
Biochemistry & Molecular Biology
Ryan J. Mailloux
Article
Cardiac & Cardiovascular Systems
Malak Almutairi, Keshav Gopal, Amanda A. Greenwell, Adrian Young, Robert Gill, Hanin Aburasayn, Rami Al Batran, Jadin J. Chahade, Manoj Gandhi, Farah Eaton, Ryan J. Mailloux, John R. Ussher
Summary: The study demonstrates that liraglutide indirectly augments myocardial glucose oxidation, potentially contributing to improved cardiovascular outcomes in people with type 2 diabetes.
CANADIAN JOURNAL OF CARDIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Ryan J. Mailloux
Article
Biochemistry & Molecular Biology
Jonathan Hirschenson, Ryan J. Mailloux
Summary: The study demonstrates that glutathionylation can enhance ROS production by mitochondria, with different effects depending on the oxidizing sources and inhibitors used.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ryan J. Mailloux
Summary: Mitochondria primarily produce superoxide and hydrogen peroxide, with the latter being considered the main ROS emitted into the cell. While H2O2 is now recognized as a central component in redox signaling, it has been suggested that superoxide may also act as a signal in mammalian cells.
Article
Biochemistry & Molecular Biology
Megan Letourneau, Kevin Wang, Ryan J. Mailloux
Summary: The study demonstrates that protein S-glutathionylation can inhibit ROS production by cytoplasmic and mitochondrial enzymes, with glutathionylation reactions being enzymatically mediated by GST. Additionally, it was found that bacterial XO is also a target for glutathionylation.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Jonathan Hirschenson, Emiliano Melgar-Bermudez, Ryan J. Mailloux
Summary: Mitochondrial uncoupling proteins (UCP) 1-3 have multiple physiological functions, and most studies suggest that they play a role in preventing oxidative stress. The exact mechanism is still unclear, but it may involve proton return to the matrix and other functions such as Ca2+ transport.
Article
Biochemistry & Molecular Biology
Kevin Wang, Jonathan Hirschenson, Amanda Moore, Ryan J. Mailloux
Summary: This study found that the glutathionylation of mitochondrial complex I can lead to increased production of ROS during reverse electron transfer from the ubiquinone pool. This has implications for understanding the relationship between mitochondrial glutathionylation reactions and oxidative stress induction in various pathologies.
Review
Cell Biology
Ryan J. J. Mailloux, Cathryn Grayson, Olivia Koufos
Summary: Protein S-glutathionylation has been proposed as a regulator of cell metabolism for four decades. This redox-sensitive covalent modification serves as a cell-wide signaling platform and plays a key role in embryonic development and regulation of physiological functions. It functions as a feedback loop for inhibiting mitochondrial hydrogen peroxide (H2O2) production and desensitizing mitochondrial redox signals.
Article
Biochemistry & Molecular Biology
Richard B. B. Richardson, Ryan J. J. Mailloux
Summary: This article discusses the interactions between circadian biorhythms, oxidative stress, metabolism, and thermoregulation. Posttranslational modifications of proteins and mitochondrial repair play a crucial role in the sleep-wake cycles and the health of the nucleus and mitochondria. The hypothesis adds to the understanding of mitochondrial regulation and the pathogenesis of various diseases.
Article
Biochemistry & Molecular Biology
Robert Gill, Sarah Mallay, Adrian Young, Ryan J. Mailloux
Article
Biochemistry & Molecular Biology
Sarah Mallay, Robert Gill, Adrian Young, Ryan J. Mailloux
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)