Article
Agriculture, Multidisciplinary
Xianlin Han, Hongping Ye
Summary: Although triglycerides play important roles in cellular growth, metabolism, and function, accurately identifying their molecular species and quantities remains a challenge.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Immunology
Wenqian Zhang, Hongjun Zhao, Pei Du, Haobo Cui, Shuang Lu, Zhongyuan Xiang, Qianjin Lu, Sujie Jia, Ming Zhao
Summary: This study identified biomarkers associated with different clinical phenotypes in SLE patients, which could facilitate a more precise diagnosis and assessment of disease progression in SLE.
CLINICAL IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Kimberly C. Fabijanczuk, James W. Hager, Scott A. McLuckey
Summary: Conventional electrospray ionization (ESI) of mixtures can complicate the analysis of individual components due to the overlap of singly and multiply charged analyte species. To address this challenge, a gas-phase approach was employed to separate singly charged ions from a population of ions of mixed charge states. The method allows for improved signal-to-noise ratio and retention of spatially separated ions within a mass spectrometer.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Michal A. Surma, Mathias J. Gerl, Ronny Herzog, Jussi Helppi, Kai Simons, Christian Klose
Summary: Lipidomics plays a crucial role in research, revealing the uniqueness of lipid compositions in different cell types and organs through studies based on mice. This study on mouse lipidomes provides insights for experimental design and interpretation of lipidomic datasets.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Shanshan Huang, Dong Zhang, Qian Wang, Bo Shang, Jianlei Liu, Xiaoting Xing, Yu Hong, Xiaoliang Duan, Hui Sun
Summary: This study investigated the changes in phospholipid molecular species in four varieties of brown rice during accelerated aging. The results showed that the content of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylglycerol (PG) gradually decreased in japonica rice, but showed no difference in indica rice during accelerated aging. The findings provide insights into the impact of accelerated aging on phospholipids of brown rice.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Cell Biology
Nai-Kui Liu, Ling-Xiao Deng, Miao Wang, Qing-Bo Lu, Chunyan Wang, Xiangbing Wu, Wei Wu, Ying Wang, Wenrui Qu, Qi Han, Yongzhi Xia, Baylen Ravenscraft, Jin-Lian Li, Si-Wei You, Peter Wipf, Xianlin Han, Xiao-Ming Xu
Summary: Alterations in cardiolipin (CL), a family of mitochondrion-specific phospholipids, have been found to play a crucial role in mitochondrial dysfunction and neuronal death following spinal cord injury (SCI). CL changes in the early stage of SCI lead to decreased abundance of CL species and oxidative stress, resulting in mitochondrial dysfunction, neuronal death, tissue damage, and motor deficits. Pharmacologic inhibition of CL alterations with a mitochondria-targeted scavenger can protect against these effects.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Jun Fu, Jixun Li, Yuzhen Sun, Shu Liu, Fengrui Song, Zhongying Liu
Summary: This study investigated the therapeutic effects and mechanism of action of the polysaccharide SCP2 from Schisandra chinensis on AD rats. The results showed that SCP2 improved cognitive dysfunction, oxidative damage, and A beta deposition in the hippocampus. Metabolomics analysis revealed that SCP2 reversed the metabolic profile disorder in AD rats, identifying key metabolites involved in fatty acid metabolism. These findings provide new insights into the potential of SCP2 for treating AD and lay a foundation for future structure-activity studies.
Article
Cell Biology
Edilburga Reyes-Jimenez, Alma Aurora Ramirez-Hernandez, Jovito Cesar Santos-Alvarez, Juan Manuel Velazquez-Enriquez, Socorro Pina-Canseco, Rafael Baltierrez-Hoyos, Veronica Rocio Vasquez-Garzon
Summary: Pulmonary fibrosis is a chronic progressive disease with high incidence rates worldwide. Oxidative stress, characterized by overproduction of 4-HNE, plays an important role in the pathogenesis of pulmonary fibrosis, with complex cellular and molecular mechanisms involved.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Chemistry, Analytical
David S. Gertner, Jake P. Violi, David P. Bishop, Matthew P. Padula
Summary: Due to the complexity of lipids, in silico generated spectral libraries have become crucial for identifying lipid species in mass spectral data. However, many existing libraries have limitations in usability and representativeness. To address this issue, we introduce Lipid Spectrum Generator, an open-source tool specifically designed for aiding lipid identification in liquid chromatography-tandem mass spectrometry analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Immunology
Maren Rudolph, Yuting Wang, Theresa Simolka, Emilie Huc-Claustre, Lingyun Dai, Gijsbert Grotenbreg, Gurdyal Singh Besra, Anna Shevchenko, Andrej Shevchenko, Sebastian Zeissig
Summary: Researchers have developed a novel system to analyze CD1d-bound lipids in mammalian cells and have gained new insights into the spectrum of CD1d-associated lipids, with important functional implications for NKT cell activation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lei Lei, Jun Yang, Jianan Zhang, Guodong Zhang
Summary: Oxidative stress is a significant contributor to the pathogenesis of colorectal cancer, with the lipid peroxidation product EKODE playing a crucial role in colonic inflammation and colon tumorigenesis.
Review
Pharmacology & Pharmacy
Yeqing Hu, Qiguo Wu, Yulin Wang, Haibo Zhang, Xueying Liu, Hua Zhou, Tao Yang
Summary: This review summarizes the mechanisms of TP-induced hepatotoxicity, involving factors such as CYP450s, P-glycoprotein (P-gp), oxidative stress, excessive autophagy, apoptosis, metabolic disorders, immunity, and the gut microbiota. These new findings contribute to a comprehensive understanding of TP-induced hepatotoxicity and detoxification.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Applied
Chuan-hai Tu, Xue-er Qi, Shan-shan Shui, Hui-min Lin, Soottawat Benjakul, Bin Zhang
Summary: The study showed that the oxidative effects of hydroxyl radical significantly increased the peroxide value and thiobarbituric acid index in shrimp muscle, altering the lipidomics profiles. Certain lipids could serve as promising biomarkers for distinguishing fresh and oxidation-treated shrimp products.
Article
Biochemical Research Methods
Lan Wang, Minghui Zhu, Yan Li, Peishuo Yan, Zhongzheng Li, Xiuping Chen, Juntang Yang, Xin Pan, Huabin Zhao, Shenghui Wang, Hongmei Yuan, Mengxia Zhao, Xiaogang Sun, Ruyan Wan, Fei Li, Xiaobo Wang, Hongtao Yu, Ivan Rosas, Chen Ding, Guoying Yu
Summary: This study identified specific proteins and patterns associated with IPF's clinical parameters using serum proteomic dataset analysis. Ageing was found to be a critical risk factor for IPF, and expression of LDHA and CCT6A correlated with elevated serum lactic acid levels in IPF patients. A combination biomarker accurately distinguished IPF patients from healthy individuals. These findings provide important insights for the diagnosis and treatment decisions of IPF.
MOLECULAR & CELLULAR PROTEOMICS
(2023)
Article
Biochemistry & Molecular Biology
Jianya Xu, Huafeng Fang, Ying Chong, Lili Lin, Tong Xie, Jianjian Ji, Cunsi Shen, Chen Shi, Jinjun Shan
Summary: This study aimed to examine the teratogenicity of Cyclophosphamide (CP) on pregnant rats. It was found that CP treatment led to the accumulation of oxidized lipids in the amniotic fluid and a decrease in certain metabolites. This suggests that CP may exert toxic effects on pregnant rats through oxidative stress.
Article
Immunology
Jia Zhou, Feilong Lu, Shan Li, Meijuan Xie, Haimei Lu, Zhijun Xie, Dehong Wu, Shuang Wang, Chengping Wen, Zheng-Hao Xu
INFLAMMOPHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Huiqing Lv, Changfeng Hu, Zhijun Xie, Ping Wang, Xuezhi Chen, Chengping Wen
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Long Xu, Changfeng Hu, Yongguo Liu, Siming Li, Walter Vetter, Huiyong Yin, Yonghua Wang
JOURNAL OF LIPID RESEARCH
(2020)
Article
Pharmacology & Pharmacy
Qianqian Li, Jia Zhou, Dingyi Zhang, Xiafeng Zhang, Zhenghao Xu, Dehong Wu
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Chemistry, Analytical
Changfeng Hu, Miao Wang, Qiao Duan, Xianlin Han
ANALYTICA CHIMICA ACTA
(2020)
Review
Spectroscopy
Changfeng Hu, Wenqing Luo, Jie Xu, Xianlin Han
Summary: Lipid research is gaining more attention, and mass spectrometry-based lipidomics approaches are powerful tools for analyzing cellular lipidomes. However, artifacts generated from in-source fragmentation can cause problems in lipidomics analysis, leading to misannotation and false identification. This article summarizes the manifestations of ion source-generated artifacts in three representative lipidomics approaches and discusses strategies to avoid/reduce these artifacts.
MASS SPECTROMETRY REVIEWS
(2022)
Article
Biochemical Research Methods
Jiajia Liu, Dingyi Zhang, Keer Wang, Zhengfu Li, Zhaochun He, Dehong Wu, Zhenghao Xu, Jia Zhou
Summary: Metabolomics analysis using GC/MS revealed metabolic alterations in MRL/lpr mice at different stages of SLE development, primarily involving pathways such as the TCA cycle, glycolysis, and butanoate metabolism. These findings could serve as a reference for studying the mechanism of SLE and assessing the pathological stage and severity of the disease.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Changfeng Hu, Jida Zhang, Suzhen Hong, Haichang Li, Lu Lu, Guanqun Xie, Wenqing Luo, Yu Du, Zhijun Xie, Xianlin Han, Chengping Wen
Summary: The close relationships among oxidative stress, dyslipidaemia, inflammation, and autoimmune response in SLE patients contribute to dysfunction and dysregulation of immunocytes. Aberrant lipid metabolism in immunocytes, induced by oxidative stress, may lead to increased production of IgG autoantibodies and cytokines in the early stages of the disease. Treatment with a natural antioxidant can correct the aberrant lipid metabolism and reduce autoimmune reactions, suggesting a potential drug treatment for SLE with fewer adverse effects.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Changfeng Hu, Yu Du, Xiaofen Xu, Haichang Li, Qiao Duan, Zhijun Xie, Chengping Wen, Xianlin Han
Summary: The study found that in the development of LN, there were always ectopic fat deposition and abnormal lipid metabolism in the kidneys, related to oxidative stress. Anti-inflammatory FAHFA species in kidney tissues could largely reflect the severity of LN and could be a potential early biomarker. Treatment with GCs could prevent LN progression but worsen lipid metabolism aberrances, especially with long-term use.
Article
Immunology
Huawei Zhao, Jia Zhou, Haimei Lu, Anran Xi, Mengxian Luo, Keer Wang, Hongjie Lv, Huijuan Wang, Ping Wang, Jing Miao, Zhenghao Xu
Summary: The study found that pretreatment with azithromycin exacerbated TMA-induced AD-like symptoms, promoting the development of ear thickening and the severity of AD symptoms. Azithromycin pretreatment also promoted the infiltration of neutrophil-like cells, T cells, and mast cells in the ear skin. In addition, azithromycin pretreatment increased the levels of IL-4, IL-6, and IL-17A in the ear skin, but increased serum TNF-alpha and IL-6. Furthermore, azithromycin pretreatment altered the composition of gut microbiota.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jida Zhang, Lu Lu, Xiaoyu Tian, Kaili Wang, Guanqun Xie, Haichang Li, Chengping Wen, Changfeng Hu
Summary: Cardiac involvement is a common symptom in systemic lupus erythematosus (SLE) and contributes to mortality. The exact pathological mechanism for cardiac involvement in lupus is not well understood. Lipid metabolism abnormalities and inflammation are associated with cardiovascular disease (CVD) development. This study quantitatively analyzed cellular lipids in cardiac tissue of lupus-prone mice and found that lipid alterations induced by inflammation were present even before lupus onset, which could further accelerate CVD. Treatment with glucocorticoids (GCs) delayed SLE progression and partially corrected lipid abnormalities. Medications with better anti-inflammatory effects may be useful for treating premature CVD in SLE.
Editorial Material
Gastroenterology & Hepatology
Wu-Meng Jin, Si-Yue Song, Xiao-Fen Xu, Cheng-Ping Wen, Chang-Feng Hu
HEPATOBILIARY & PANCREATIC DISEASES INTERNATIONAL
(2022)
Article
Pharmacology & Pharmacy
Yitian Zhu, Luyun Zhang, Xiafeng Zhang, Dehong Wu, Leiming Chen, Changfeng Hu, Chengping Wen, Jia Zhou
Summary: This study investigates the therapeutic efficacy of Tripterygium wilfordii glycosides (TWG) and its effect on lipid metabolism in rheumatoid arthritis (RA). The results show that TWG effectively ameliorates arthritis in a CIA rat model and corrects lipid abnormalities associated with arthritis severity and IL-1β level. These findings provide new insights into the role and mechanism of TWG in the treatment of RA.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Chemistry, Applied
Changfeng Hu, Jingyi Song, Wumeng Jin, Wenqiang Wang, Hongjin Bai, Cuiyun Wu, Lirong Shen
Summary: Walnut oil, rich in polyunsaturated fatty acids, has many health benefits. The synthesis and accumulation of walnut oil in the embryo development process may be influenced by a special mechanism for triacylglycerol biosynthesis. Shotgun lipidomics analysis revealed that triacylglycerol synthesis in walnut kernels occurred before 84 days after flowering and was enhanced between 84 and 98 days. Acyl editing through phosphatidylcholine played a role in the flux of fatty acids for triacylglycerol synthesis. Therefore, triacylglycerol biosynthesis in walnut kernels is directly linked to lipid metabolism.
Article
Engineering, Environmental
Mingying Liu, Ju Mu, Miao Wang, Changfeng Hu, Jinjun Ji, Chengping Wen, Dayi Zhang
Summary: Microplastics (MPs) accumulation and its impacts on lipid metabolism in mouse liver were investigated in this study using lipidomics and Raman spectroscopy. The results showed that polypropylene-MPs induced lipid droplet accumulation in the liver and significantly altered lipid metabolic profiles, including triglycerides, fatty acids, free fatty acids, and lysophosphatidylcholine. The presence of polypropylene-MPs also affected membrane properties and cellular lipid storage and oxidation regulation. The findings provide insights into the toxicities and risks of polypropylene-MPs to mammals.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)