4.7 Article

Effect of red light on the composition of metabolites in tea leaves during the withering process using untargeted metabolomics

Journal

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 102, Issue 4, Pages 1628-1639

Publisher

WILEY
DOI: 10.1002/jsfa.11500

Keywords

tea; red light withering; non-volatile compounds; metabolomics; amino acids; theaflavins

Funding

  1. earmarked fund for China Agriculture Research System [CARS-19]
  2. Zhejiang Province agricultural major technology cooperative extension project [2020XTTGCY02-03]
  3. Sichuan Province Science and Technology Planning Project [2018NZ0031]
  4. Central Public Interest Scientific Institution Basal Research Fund [1610212018002]

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This study comprehensively analyzed the metabolites in withered tea leaves under red light and dark conditions, revealing that amino acids, catechins, and dimeric catechins are most affected by red light treatment. The results provide insight into the dynamic effects of red light on tea withering and its application in quality control during tea processing.
BACKGROUND Red light withering significantly improves the sensory flavor qualities of tea, although changes in metabolites during this process have not been systematically studied until now. The present study comprehensively analyzes metabolites in withered tea leaves at 2-h intervals up to 12 h under red light (630 nm) and dark conditions using ultra performance liquid chromatography-high resolution mass spectrometry (untargeted metabolomics). RESULTS Ninety-four non-volatile compounds are identified and relatively quantified, including amino acids, catechins, dimeric catechins, flavonol glycosides, glycosidically-bound volatiles, phenolic acids and nucleosides. The results show that amino acids, catechins and dimeric catechins are most affected by red light treatment. Ten free amino acids, theaflavins and theasinensin A increase after red light irradiation, whereas epigallocatechin gallate and catechin fall. CONCLUSION The present study provides a comprehensive and systematic profile of the dynamic effects of red light on withering tea and a rationale for its use in tea processing quality control. (c) 2021 Society of Chemical Industry.

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