Article
Chemistry, Multidisciplinary
Ouyang Dan, Chuanzhe Wang, Chao Zhong, Juan Lin, Gang Xu, Guane Wang, Zian Lin
Summary: In this study, novel LDI-MS substrates were designed to enhance the sensitivity of mass signals and achieve high accuracy diagnosis of different saccharides and central precocious puberty (CPP).
Article
Chemistry, Analytical
Manxi Yang, Daisy Unsihuay, Hang Hu, Frederick Nguele Meke, Zihan Qu, Zhong-Yin Zhang, Julia Laskin
Summary: Mass spectrometry imaging (MSI) is a powerful technique for mapping the spatial distribution of proteins in biological tissues. In this study, we demonstrate proteoform mapping in biological tissues using nano-DESI MSI, achieving a spatial resolution down to 7 μm. We have improved the sensitivity of nano-DESI MSI experiments by optimizing the design of the capillary-based probe and the thickness of the tissue section. These developments represent a new step in MSI-based spatial proteomics and complement targeted imaging modalities used for studying biological systems.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Lanaia Itala Louzeiro Maciel, Ricardo Alves Bernardo, Rafael Oliveira Martins, Almir Custodio Batista Jr, Joao Victor Ataide Oliveira, Andrea Rodrigues Chaves, Boniek Gontijo Vaz
Summary: The imaging of biological tissues using mass spectrometry imaging (MSI) techniques allows for the visualization of the distribution of various metabolites, drugs, lipids, and glycans in complex samples. MSI methods like DESI-MSI and MALDI-MSI provide high sensitivity and the ability to evaluate/visualize multiple analytes in a single sample, overcoming limitations of classical microscopy techniques. This review discusses the application of DESI-MSI and MALDI-MSI in evaluating exogenous and endogenous molecules in biological samples, providing technical insights and recent research findings.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Food Science & Technology
Qi Wang, Xiaohui Li, Hongping Wang, Simeng Li, Chen Zhang, Xueying Chen, Jing Dong, Hua Shao, Jing Wang, Fen Jin
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) method was developed to obtain dynamic images of forchlorfenuron in oriental melons. The majority of forchlorfenuron was found in the exocarp and mesocarp regions of the melons and decreased within two days after application. The degradation rate obtained by MALDI-MSI was reliable and comparable to HPLC-MS/MS, indicating its practicability for pesticide degradation study.
Article
Chemistry, Applied
Hirofumi Enomoto, Shiro Takeda, Hajime Hatta
Summary: This study utilized MALDI-MSI to investigate the distribution of PI molecular species in pork chop, revealing tissue-specific patterns with varying compositions of PI molecular species related to fatty acid profiles. This suggests a potential association between individual pork tissues and the protective effects of PI molecular species against pregnancy and cancer-related diseases.
JOURNAL OF OLEO SCIENCE
(2021)
Article
Multidisciplinary Sciences
Hua Zhang, Lin Zhao, Jingjing Jiang, Jie Zheng, Li Yang, Yanyan Li, Jian Zhou, Tianshu Liu, Jianmin Xu, Wenhui Lou, Weige Yang, Lijie Tan, Weiren Liu, Yiyi Yu, Meiling Ji, Yaolin Xu, Yan Lu, Xiaomu Li, Zhen Liu, Rong Tian, Cheng Hu, Shumang Zhang, Qinsheng Hu, Yangdong Deng, Hao Ying, Sheng Zhong, Xingdong Zhang, Yunbing Wang, Hua Wang, Jingwei Bai, Xiaoying Li, Xiangfeng Duan
Summary: As cancer is increasingly seen as a metabolic disorder, serum metabolite profiling is proposed as a viable method for detecting cancer. In this study, a machine learning model using mass spectrometry-based liquid biopsy data is reported for pan-cancer screening and classification. The Multiplexed Nanomaterial-Assisted LDI for Cancer Identification (MNALCI) achieves high sensitivity and specificity for distinguishing cancers from healthy controls and accurately identifying the tissue of origin of tumors. This high throughput assay shows promising potential for non-invasive cancer diagnosis.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Xian-Na Wang, Bin Li
Summary: Gold nanoparticles/thiol-beta-cyclodextrin-functionalized TiO2 nanowires were prepared to enhance the performance of SALDI MS and MSI, showing superior detection sensitivity and repeatability.
ANALYTICAL CHEMISTRY
(2022)
Review
Plant Sciences
Patrick J. Horn, Kent D. Chapman
Summary: Mass spectrometry imaging (MSI) is a valuable analytical technique used to investigate the spatial distribution of molecules within biological systems. In the realm of plant science, MSI has been increasingly employed to explore the distribution patterns of diverse metabolites and phytochemicals, facilitating the understanding of their metabolic pathways and functional roles.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Biochemistry & Molecular Biology
Andreas Mund, Andreas-David Brunner, Matthias Mann
Summary: Mass spectrometry-based proteomics is a powerful technology for quantifying proteins in cells or tissues. Single-cell transcriptomes are influenced by stochastic noise due to low transcript numbers, whereas single-cell proteomes appear to be more complete. With the combination of imaging and spatial transcriptomics, the spatial organization of cells in tissues can be studied alongside ultra-sensitive proteomics.
Article
Biochemistry & Molecular Biology
Sangwon Cha, Gyouwoong Jun, Yougyeong Park, Sung Jun An, Donghoon Lee
Summary: Nanospray desorption electrospray ionization (nano-DESI) was used to analyze the metabolite fingerprints on Hypericum leaves. The intensity changes of key metabolites over time were observed through full mass scan experiments and monitored using selected ion monitoring and pseudo-selected reaction monitoring experiments. The correlation between the time-dependent changes in the metabolite fingerprints and depth-wise metabolite distribution was examined by removing the surface layers of the leaves multiple times.
Article
Chemistry, Analytical
Xufang Hu, Zongping Wang, Haolin Chen, An Zhao, Nianrong Sun, Chunhui Deng
Summary: An advanced metabolic analysis tool based on highly porous metal oxides derived from metal-organic frameworks (MOFs) is developed in this study. The tool successfully differentiates and stages renal cell carcinoma (RCC), as well as identifies the size of tumors. This achievement shows promising prospects for designer matrix-based analytical tools in disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Yukun Liu, Xiaofei Nie, Jilong Wang, Zhenqi Zhao, Zhimei Wang, Fang Ju
Summary: Flavonoids in litchi seeds have broad-spectrum antiviral and antitumor activities, especially against breast and liver cancers. MALDI-MSI was used to detect and image the distribution of flavonoids in litchi seed tissue sections for the first time, revealing their heterogeneous distribution and potential for extraction.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Ge Ma, Xiaoyong Zhao, Mengzhe Guo, Yaqin Liu, Keqiang Shi, Cheng Guo, Yuanjiang Pan
Summary: In this study, a matrix glycosylation strategy was proposed to improve the sensitivity and signal reproducibility of MALDI MS analysis. The glycosylated matrices, especially 6-GAQ, exhibited remarkably-improved performance in detecting metabolites and imaging small molecules in complex samples. The glycosylation strategy showed great potential in the field of mass spectrometry.
ANALYTICA CHIMICA ACTA
(2022)
Review
Chemistry, Analytical
Katerina V. Djambazova, Jacqueline M. Van Ardenne, Jeffrey M. Spraggins
Summary: Imaging mass spectrometry allows for the untargeted mapping of biomolecules directly from tissue sections, with widespread clinical applicability in disease diagnosis and classification. This review summarizes the two most widely employed IMS technologies and their advancements in biomedical applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Microbiology
Anil K. Persad, Hanan A. Fahmy, Nicholas Anderson, Jing Cui, Zeynal Topalcengiz, Saharuetai Jeamsripong, Patrick M. Spanninger, Robert L. Buchanan, Kalmia E. Kniel, Michele T. Jay-Russell, Michelle D. Danyluk, Gireesh Rajashekara, Jeffrey T. LeJeune
Summary: This study demonstrates that MALDI-TOF is a viable alternative for the rapid identification and differentiation of Salmonella serovars.
Article
Energy & Fuels
Limin Ren, Yehua Han, Yahe Zhang, Yanfen Zhang, Xianghai Meng, Quan Shi
Article
Energy & Fuels
Tingting Cheng, Yehua Han, Yanfen Zhang, Chunming Xu
Article
Energy & Fuels
Meng Wang, Suoqi Zhao, Limin Ren, Yehua Han, Chunming Xu, Keng H. Chung, Quan Shi
Review
Energy & Fuels
Yehua Han, Yanfen Zhang, Chunming Xu, Chang Samuel Hsu
Article
Chemistry, Physical
Jun Han, Xingyu Tang, Yida Wang, Yajie Wang, Yehua Han, Xiaohuan Lin, Xiao Dong, Hyun Hwi Lee, Haiyan Zheng, Kuo Li, Ho-kwang Mao
JOURNAL OF PHYSICAL CHEMISTRY C
(2019)
Review
Chemistry, Analytical
Yinghao Wang, Yehua Han, Wenya Hu, Dali Fu, Gang Wang
JOURNAL OF SEPARATION SCIENCE
(2020)
Article
Biochemical Research Methods
Na Li, Jing Dong, Chenglong Dong, Yehua Han, Huwei Liu, Fuyou Du, Honggang Nie
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2020)
Article
Chemistry, Analytical
Yanfen Zhang, Yehua Han, Jianxun Wu, Yinghao Wang, Jiayi Li, Quan Shi, Chunming Xu, Chang Samuel Hsu
Summary: Thiophene compounds play a crucial role in petroleum desulfurization, and APCI coupled with UHRMS and TIMS have been effectively utilized to determine the composition and structural information of these compounds, offering new insights for desulfurization studies.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Yanfen Zhang, Yehua Han, Wenya Hu, Qiong Pan, Zhanfang Liu, Guannan Ling, Quan Shi, Rui Weng
Summary: Utilizing DAGs ions as marker indicators for edible oil classification through UHRMS technology was proven to be more effective than using TAGs ions in chemometric analyses. This method can also be applied for the characterization of blend oils and gutter oils.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Physical
Peijie Zhang, Xingyu Tang, Chunfang Zhang, Dexiang Gao, Xuan Wang, Yajie Wang, Wenhan Guo, Ruqiang Zou, Yehua Han, Xiaohuan Lin, Xiao Dong, Kuo Li, Haiyan Zheng, Ho-kwang Mao
Summary: This study discovered a double hydrogen transfer process in solid 2-butyne under high pressure, which initiated polymerization. The process involved intermolecular CH...π interactions via an aromatic Hückel six-membered ring intermediate state. This finding provides important insights into hydrogen transfer in solid-state hydrocarbons.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Engineering, Chemical
Yinghao Wang, Guangyou Zhu, Meng Wang, Jianxun Wu, Dali Fu, Qingqing Xie, Quan Shi, Chunming Xu, Yehua Han
Summary: Thiaethanodiamondoids (1-7 cages) and higher ethanodiamondoids (4-7 cages), two categories of diamondoid compounds, were discovered and characterized for the first time. The conformers of these compounds were identified as well. This discovery was made possible through the innovative multi-dimensional mass spectrometry (MS) strategy, which also has potential for the MS characterization of unreported natural products in complex matrices.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Chemistry, Analytical
Wenya Hu, Jialin Niu, Ruoning Bao, Chenglong Dong, Habtegabir Sara Girmay, Chunming Xu, Yehua Han
Summary: Petroleum olefins, important feedstocks for the chemical industry, are challenging to analyze. This study developed a new method to selectively analyze olefins by converting them into oxetane compounds and using mass spectrometry. Additionally, a scheme was created to differentiate different types of unsaturated alkanes in complex petroleum samples.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Meixian Liu, Junliang Liu, Xianmo Gu, Peng Kong, Yehua Han, Xing Wang, Zhanfeng Zheng
Summary: During the photomineralization of phenolic pollutants using carbon nitride, the co-modification of hydroxyl groups and carbon vacancies can enhance the in-situ mineralization of phenol and prevent the formation of refractory polymers.
JOURNAL OF CATALYSIS
(2023)
Article
Chemistry, Analytical
Wenya Hu, Honggang Nie, Yinghao Wang, Na Li, Shuangshuang Di, Qiong Pan, Jikun Liu, Yehua Han
Summary: A matrix-assisted laser desorption/ionization mass spectrometry imaging method was developed to study the migration and transformation of metabolites in the xylem during heartwood formation and after mechanical injury. The method successfully identified different types of metabolites and revealed their migration and transformation mechanisms.
