4.7 Article

Rice SPL10 positively regulates trichome development through expression of HL6 and auxin-related genes

Journal

JOURNAL OF INTEGRATIVE PLANT BIOLOGY
Volume 63, Issue 8, Pages 1521-1536

Publisher

WILEY
DOI: 10.1111/jipb.13140

Keywords

auxin; complementary genes; hairy leaves; Oryza sativa; OsWOX3B

Funding

  1. National Science Foundation of China [31271689]
  2. Ministry of Science and Technology of China [2016YFD0100101-09]

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Trichomes play essential roles in plant defense against various stresses, with OsSPL10 and OsWOX3B identified as key regulators of trichome development in rice. OsSPL10, a transcriptional regulator, controls trichome development primarily through allelic variation in its promoter region. It also affects the expression of other genes related to trichome development and influences feeding behavior of herbivores.
Trichomes function in plant defenses against biotic and abiotic stresses; examination of glabrous lines, which lack trichomes, has revealed key aspects of trichome development and function. Tests of allelism in 51 glabrous rice (Oryza sativa) accessions collected worldwide identified OsSPL10 and OsWOX3B as regulators of trichome development in rice. Here, we report that OsSPL10 acts as a transcriptional regulator controlling trichome development. Haplotype and transient expression analyses revealed that variation in the approximately 700-bp OsSPL10 promoter region is the primary cause of the glabrous phenotype in the indica cultivar WD-17993. Disruption of OsSPL10 by genome editing decreased leaf trichome density and length in the NIL-HL6 background. Plants with genotype OsSPL10(WD-17993)/HL6 generated by crossing WD-17993 with NIL-HL6 also had fewer trichomes in the glumes. HAIRY LEAF6 (HL6) encodes another transcription factor that regulates trichome initiation and elongation, and OsSPL10 directly binds to the HL6 promoter to regulate its expression. Moreover, the transcript levels of auxin-related genes, such as OsYUCCA5 and OsPIN-FORMED1b, were altered in OsSPL10 overexpression and RNAi transgenic lines. Feeding tests using locusts (Locusta migratoria) demonstrated that non-glandular trichomes affect feeding by this herbivore. Our findings provide a molecular framework for trichome development and an ecological perspective on trichome functions.

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